Passive RFID has the advantages of rapid identification of multi-target objects and low implementation cost. It is the most critical technology in the Industrial Internet of Things information-gathering layer and is extensively applied in various industries, such as smart production, asset management, and monitoring. The signal collision caused by the communication between the reader/writer and tags sharing the same wireless channel has caused a series of problems, such as the reduction of the identification efficiency of the reader/writer and the increment of the missed reading rate, thus restricting the further development of RFID. At present, many hybrid anti-collision algorithms integrate the advantages of Aloha and TS algorithms to optimize RFID system performance, but these solutions also suffer from performance bottlenecks. In order to break through such performance bottleneck, based on the ISE-BS algorithm, we combined the sub-frame observation mechanism and the Q value adjustment strategy and proposed two hybrid anti-collision algorithms. The experimental results show that the two algorithms proposed in this paper have obvious advantages in system throughput, time efficiency and other metrics, surpassing existing UHF RFID anti-collision algorithms.