Reproduction In Goats Research Articles

Developmental programming of reproduction in sheep and goat: Association of fraternity size and sex ratio with reproductive performance of ewes and does at the first pregnancy

Various prenatal factors including the number of littermates (fraternity size) and exposure to male littermate (fraternity sex ratio) during fetal period have been reported to influence postnatal fertility in the mammals. The present research was conducted to study the association of fraternity size and sex ratio with reproductive performance of nulliparous ewes and does. To this end, data associated with number of littermates, exposure to male littermate, birth weight, age at first pregnancy, as well as litter size, sex ratio of offspring, litter weight, and birth weight of female and male offspring after the first parturition retrieved from the database of sheep (n = 536 Romane and 289 Blanche du Massif Central ewes) and goat (n = 174 Alpine and 267 Saanen does) flocks. Fraternity size was negatively associated with birth weight of ewes and does (P < 0.05). Exposure to male littermate during fetal period was associated with younger age at first pregnancy and larger litter size in the does (P < 0.05), but not in the ewes (P > 0.05). Exposure to male littermate during fetal period was positively associated with the odds of male-biased litters in the ewes and does (P < 0.05). Fraternity size was positively associated with litter weight in the does (P < 0.05), but not in the ewes (P > 0.05). In conclusion, the present study showed that the number and sex of littermates during fetal period could impact postnatal reproduction of ewes and does. In this context, some associations, particularly those related to exposure to male littermate during fetal period, were only observed in does, which implicates that the effect of androgens on developmental programming of reproduction may be species-specific.

Transcriptome analysis reveals miRNA expression profiles in hypothalamus tissues during the sexual development of Jining grey goats

BackgroundExploring the physiological and molecular mechanisms underlying goat sexual maturation can enhance breeding practices and optimize reproductive efficiency and is therefore substantially important for practical breeding purposes. As an essential neuroendocrine organ in animals, the hypothalamus is involved in sexual development and other reproductive processes in female animals. Although microRNAs (miRNAs) have been identified as significant regulators of goat reproduction, there is a lack of research on the molecular regulatory mechanisms of hypothalamic miRNAs that are involved in the sexual development of goats. Therefore, we examined the dynamic changes in serum hormone profiles and hypothalamic miRNA expression profiles at four developmental stages (1 day (neonatal, D1, n = 5), 2 months (prepubertal, M2, n = 5), 4 months (sexual maturity, M4, n = 5), and 6 months (breeding period, M6, n = 5)) during sexual development in Jining grey goats.ResultsTranscriptome analysis revealed 95 differentially expressed miRNAs (DEMs) in the hypothalamus of goats across the four developmental stages. The target genes of these miRNAs were significantly enriched in the GnRH signalling pathway, the PI3K-Akt signalling pathway, and the Ras signalling pathway (P < 0.05). Additionally, 16 DEMs are common among the M2 vs. D1, M4 vs. D1, and M6 vs. D1 comparisons, indicating that the transition from D1 to M2 represents a potentially critical period for sexual development in Jining grey goats. The bioinformatics analysis results indicate that miR-193a/miR-193b-3p-Annexin A7 (ANXA7), miR-324-5p-Adhesion G protein-coupled receptor A1 (ADGRA1), miR-324-3p-Erbb2 receptor tyrosine kinase 2 (ERBB2), and miR-324-3p-Rap guanine nucleotide exchange factor 3 (RAPGEF3) are potentially involved in biological processes such as hormone secretion, energy metabolism, and signal transduction. In addition, we further confirmed that miR-324-3p targets the regulatory gene RAPGEF3.ConclusionThese results further enrich the expression profile of hypothalamic miRNAs in goats and provide important insights for studying the regulatory effects of hypothalamic miRNAs on the sexual development of goats after birth.

Pregnancy Establishment and Diagnosis in Livestock.

This comprehensive review explores the complex processes of reproduction, pregnancy establishment, and pregnancy diagnostic methods in cattle, sheep, goats, swine, horses, and camelids. It provides an overview of the history of pregnancy detection and an in-depth exploration of the physiology of pregnancy in livestock. The detection of conceptus tissue and fluids, conceptus-produced hormones, and maternal responses to conceptus signals, crucial for pregnancy diagnosis, are also discussed in detail, as are emerging methods for pregnancy diagnosis in livestock species. Overall, this review emphasizes the direct impact of pregnancy diagnosis and efficient pregnancy management for profitability of livestock enterprises.

Analysis of goat PPP6C mRNA profile, detection of genetic variations, and their associations with litter size

The Protein Phosphatase 6 Catalytic Subunit (PPP6C) is evolutionarily a conserved gene in eukaryotes known to play a significant role in mammalian reproduction. This study aimed to investigate expression patterns of PPP6C and explore its association with litter size in Shaanbei white cashmere (SBWC) goats. Initially, we determined the mRNA expression levels of PPP6C in both male and female goats across multiple tissues. The results showed that PPP6C mRNA was expressed in multiple tissues, with higher levels in the testis and fallopian tubes, suggesting its involvement in goat reproduction. Additionally, we identified a novel 19 bp InDel within the PPP6C gene in a population of 1030 SBWC goats, which exhibited polymorphism. Statistical analysis revealed a significant association between the19 bp InDel mutation and litter size (P < 0.05). Subsequent, bioinformatics analysis, including linkage disequilibrium (LD) block and selective scanning, highlighted the linkage tendency among most InDel loci did not stand out within B-8 block, there were still some InDel loci linked to the 19 bp within a relatively narrow region. Furthermore, comparative analysis with Bezoars, these selective signals all indicated that this gene was under higher selection pressure, implying that the 19 bp InDel locus within the PPP6C is potentially associated with domesticated traits, particularly in relation to litter size. The results of the present study suggest that the PPP6C is a vital candidate gene affecting prolificacy in goats, with implications for selective breeding programs for goat breeds.

The Identification of RPL4 as a Hub Gene Associated with Goat Litter Size via Weighted Gene Co-Expression Network Analysis.

Reproduction in goats is a highly complex and dynamic process of life regulation, involving coordinated regulation from various aspects such as central nervous system regulation, reproductive system development, oocyte maturation, and fertilized egg development. In recent years, researchers have identified numerous genes associated with goat reproductive performance through high-throughput sequencing, single-cell sequencing, gene knockout, and other techniques. However, there is still an urgent need to explore marker genes related to goat reproductive performance. In this study, a single-cell RNA sequencing dataset of oocytes (GSE136005) was obtained from the Gene Expression Omnibus (GEO) database. Weighted Gene Co-expression Network Analysis (WGCNA) was utilized to identify modules highly correlated with goat litter size. Through gene function enrichment analysis, it was found that genes within the modules were mainly enriched in adhesive junctions, cell cycle, and other signaling pathways. Additionally, the top 30 hub genes with the highest connectivity in WGCNA were identified. Subsequently, using Protein-Protein Interaction (PPI) network analysis, the top 30 genes with the highest connectivity within the modules were identified. The intersection of hub genes, key genes in the PPI network, and differentially expressed genes (DEGs) led to the identification of the RPL4 gene as a key marker gene associated with reproductive capacity in goat oocytes. Overall, our study reveals that the RPL4 gene in oocytes holds promise as a biological marker for assessing goat litter size, deepening our understanding of the regulatory mechanisms underlying goat reproductive performance.

Insemination timing influences reproductive performance rather than eCG (Equine Chorionic Gonadotropin) or synchronisation protocol in Murciano-Granadina does

Counteracting goat reproduction seasonality and ensuring acceptable fertility rates are critical points of the success of selection of synchronisation protocols. The present study aims to investigate the factors that affect the performance of specific protocols to increase the probability of pregnancy results in the Murciano-Granadina breed within the official breeding program and way in which such factors interrelate. This study evaluated 11,008 artificial inseminations carried out between 2010 and 2013, using three different synchronisation protocols and eCG combinations. A categorical regression model (CATREG) was used to determine the impact of farm, doe age, birth number, kidding type, kid number, number of alive and dead born kids, synchronisation protocol, kidding month, type of semen, kidding season, eCG concentration, and their inter-actions. Results indicated that all variables, except for the number of alive and dead kids and semen type, significantly (p < 0.05) contributed to explaining the variability in pregnancy rates. Conclusively, rather than the role of eCG levels and artificial insemination (AI) protocol separately, Farm x eCG x AI Protocol interaction and the multifactorial influences on pregnancy rates should be considered when selecting does for insemination to maximise the outcomes of reproductive performance.

Identification of genomic characteristics and selective signals in Guizhou black goat

BackgroundGuizhou black goat is one of the indigenous black goat breeds in the southwest region of Guizhou, China, which is an ordinary goat for mutton production. They are characterized by moderate body size, black coat, favorite meat quality with tender meat and lower odor, and tolerance for cold and crude feed. However, little is known about the genetic characteristics or variations underlying their important economic traits.ResultsHere, we resequenced the whole genome of Guizhou black goat from 30 unrelated individuals breeding in the five core farms. A total of 9,835,610 SNPs were detected, and 2,178,818 SNPs were identified specifically in this breed. The population structure analysis revealed that Guizhou black goat shared a common ancestry with Shaanbei white cashmere goat (0.146), Yunshang black goat (0.103), Iran indigenous goat (0.054), and Moroccan goat (0.002). However, Guizhou black goat showed relatively higher genetic diversity and a lower level of linkage disequilibrium than the other seven goat breeds by the analysis of the nucleotide diversity, linkage disequilibrium decay, and runs of homozygosity. Based on FST and θπ values, we identified 645, 813, and 804 selected regions between Guizhou black goat and Yunshang black goat, Iran indigenous goat, and cashmere goats. Combined with the results of XP-EHH, there were 286, 322, and 359 candidate genes, respectively. Functional annotation analysis revealed that these genes are potentially responsible for the immune response (e.g., CD28, CD274, IL1A, TLR2, and SLC25A31), humility-cold resistance (e.g., HBEGF, SOSTDC1, ARNT, COL4A1/2, and EP300), meat quality traits (e.g., CHUK, GAB2, PLAAT3, and EP300), growth (e.g., GAB2, DPYD, and CSF1), fertility (e.g., METTL15 and MEI1), and visual function (e.g., PANK2 and NMNAT2) in Guizhou black goat.ConclusionOur results indicated that Guizhou black goat had a high level of genomic diversity and a low level of linkage disequilibrium in the whole genome. Selection signatures were detected in the genomic regions that were mainly related to growth and development, meat quality, reproduction, disease resistance, and humidity-cold resistance in Guizhou black goat. These results would provide a basis for further resource protection and breeding improvement of this very local breed.

Optimization of Sustainable Goat Farm Productivity Improvement through Complete Feed Introduction Technology in Kaliploso Village, Banyuwangi

Increasing the level of the community economy through the livestock sector is increasingly in demand. The livestock sector is experiencing an increase in interest in one of them is goat farming. Not much different from the success factor of increasing the productivity of livestock, the success of goat livestock is also influenced by several factors including genetics, feed, and maintenance management. The role of farmers is necessary to achieve the desired level of productivity success. Breeders in the group Banyuwangi Goat Breeder must be prepared to be a breeder who is aware of the mechanism of raising a good right. Therefore, the education of farmers to be related to the way or technique of raising goats is good and right, goat cultivation management in the form of types of goats, goat reproduction, goat health to the manufacture of complete feed that is right must be understood by farmers. The purpose of this activity is to improve farmers ' understanding of the importance of understanding the basic aspects of breeding including genetic, feed, and maintenance management. In addition to aiming to improve farmers ' understanding of goat breeding techniques, this extension is expected to also be a solution in solving economic and social problems, especially in the field of goat farming, and independently farmers can make complete feed independently. However, the sustainability of coaching is expected to improve the quality of farmers in raising livestock and as a mechanism for evaluating the complete feed until the formation of a commercial and viable complete feed. Feed model as a source of livestock nutrition.

Comprehensive analyses of 435 goat transcriptomes provides insight into male reproduction

A systematic analysis of genes related to reproduction is crucial for obtaining a comprehensive understanding of the molecular mechanisms that underlie male reproductive traits in mammals. Here, we utilized 435 goat transcriptome datasets to unveil the testicular tissue-specific genes (TSGs), allele-specific expression (ASE) genes and their uncharacterized transcriptional features related to male goat reproduction. Results showed a total of 1790 TSGs were identified in goat testis, which was the most among all tissues. GO enrichment analyses suggested that testicular TSGs were mainly involved in spermatogenesis, multicellular organism development, spermatid development, and flagellated sperm motility. Subsequently, a total of 95 highly conserved TSGs (HCTSGs), 508 middle conserved TSGs (MCTSGs) and 42 no conserved TSGs (NCTSGs) were identified in goat testis. GO enrichment analyses suggested that the HCTSGs and MCTSGs has a more important association with male reproduction than NCTSGs. Additionally, we identified 644 ASE genes, including 88 tissue-specific ASE (TS-ASE) genes (e.g., FSIP2, TDRD9). GO enrichment analyses indicated that both ASE genes and TS-ASE genes were associated with goat male reproduction. Overall, this study revealed an extensive gene set involved in the regulation of male goat reproduction and their dynamic transcription patterns. Data reported here provide valuable insights for a further improvement of the economic benefits of goats as well as future treatments for male infertility.

The role of GnRH in Tibetan male sheep and goat reproduction.

The hypothalamic-pituitary-gonadal (HPG) axis connects the hypothalamus, pituitary gland, and gonads. The regulation of reproductive processes includes integrating various factors from structural functions and environmental conditions in the HPG axis, with the outcome indication of these processes being the pulsatile secretion of gonadotropin-releasing hormone (GnRH) from the hypothalamus. These factors include feed consumption and nutritional condition, sex steroids, season/photoperiod, pheromones, age, and stress. GnRH pulsatile secretion affects the pattern of gonadotropin secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which then regulates both endocrine function and gamete maturation in the gonads. This regulates gonadotropins and testosterone (T) production. There is evidence that in males, GnRH participates in a variety of host behavioural and physiological processes such as the release of reproductive hormones, progression of spermatogenesis and sperm function, aggressive behaviour, and physiological metabolism. GnRH activates receptors expressed on Leydig cells and Sertoli cells, respectively to stimulate T secretion and spermatogenesis in the testis. Photoperiod affects the reproductive system of the hypothalamic-pituitary axis via rhythmic diurnal melatonin secretion. Increased release of melatonin promotes sexual activity, GnRH production, LH stimulation, and T production. This induces testicular functions, spermatogenesis, and puberty. GnRH reduces the release of LH by the pituitary through the cascade effect and decreases plasma concentration of T. Gut microbiota maintain sex steroid homeostasis and may induce reduction in reproduction productivity. Recently, findings of kisspeptin-neurokinin-dynorphin neuronal network in the brain have resulted in fast advances in how GnRH secretion is controlled. Emerging studies have also indicated that other neuropeptide analogues could be used in control reproduction procedures in various goat and sheep breeds. The Tibetan male sheep and goats reproduce on a seasonal basis and have high reproductive performance. This is a review for the role of GnRH in Tibetan male sheep and goats reproduction. This is intended to enhance reproductive knowledge for understanding the key roles of GnRH relating to male reproductive efficiency of Tibetan sheep or goats.

Transcriptome sequencing reveals the effects of circRNA on testicular development and spermatogenesis in Qianbei Ma goats.

Circular RNAs (circRNAs) play an important role in regulating the mammalian reproductive system, especially testicular development and spermatogenesis. However, their functions in testicular development and spermatogenesis in the Qianbei Ma goat, the Guizhou endemic breed are still unclear. In this study, tissue sectioning and circRNAs transcriptome analysis were conducted to compare the changes of morphology and circular RNAs gene expression profile at four different developmental stages (0Y, 0-month-old; 6Y, 6-month-old; 12Y, 12-month-old; 18Y, 18-month-old). The results showed that the circumferences and area of the seminiferous tubule gradually increased with age, and the lumen of the seminiferous tubule in the testis differentiated significantly. 12,784 circRNAs were detected from testicular tissues at four different developmental stages by RNA sequencing, and 8,140 DEcircRNAs (differentially expressed circRNAs) were found in 0Y vs. 6Y, 6Y vs. 12Y, 12Y vs. 18Y and 0Y vs. 18Y, 0Y vs. 12Y, 6Y vs. 18Y Functional enrichment analysis of the source genes showed that they were mainly enriched in testicular development and spermatogenesis. In addition, the miRNAs and mRNAs associated with DECircRNAs in 6 control groups were predicted by bioinformatics, and 81 highly expressed DECircRNAs and their associated miRNAs and mRNAs were selected to construct the ceRNA network. Through functional enrichment analysis of the target genes of circRNAs in the network, some candidate circRNAs related to testicular development and spermatogenesis were obtained. Such as circRNA_07172, circRNA_04859, circRNA_07832, circRNA_00032 and circRNA_07510. These results will help to reveal the mechanism of circRNAs in testicular development and spermatogenesis, and also provide some guidance for goat reproduction.

Diluent pH affects sperm motility via GSK3 α/β-hexokinase pathway for the efficient enrichment of X-sperm to increase the female kids rate of dairy goats

Dairy goats are the goats bred with the ability to produce large quantities of milk, and the increase of the female kid rate of breeding dairy goats is beneficial for milk production and economic benefits of dairy goat farms. Our previous study revealed that regulating the pH of dairy goat semen diluent to 6.2 or 7.4 respectively, the proportion of X chromosome bearing sperm (X-sperm) in the up and down layers of the tube after incubation was significantly higher than that of Y chromosome bearing sperm (Y-sperm) i.e. enriched X-sperm. In this study, fresh dairy goat semen collected in different seasons was diluted in different pH solutions to calculate the number and rate of X-sperm and to measure the functional parameters of enriched sperm. The artificial insemination experiments were performed with enriched X-sperm. The mechanisms of regulating the pH of diluent affecting sperm enrichment were further studied. The results showed that the proportion of enriched X-sperm in pH 6.2 and 7.4 diluents of sperm collected in different seasons showed no significantly different, but were significantly higher than that of the control group (pH 6.8). The in vitro functional parameters of X-sperm enriched in pH 6.2 and 7.4 diluent solution were not significantly different from those of the control group (P > 0.05). After artificial insemination with X-sperm enriched in pH7.4 diluent, the proportion of female offspring was significantly higher than that of the control group. It was found that the regulating pH of the diluent affected sperm mitochondrial activity and glucose uptake capacity via phosphorylating NF-κB and GSK3α/β proteins. The motility activity of X-sperm was enhanced under acidic conditions and weakened under alkaline conditions, which was conducive to the effective enrichment of X-sperm. This study demonstrated that the number and proportion of X-sperm enriched using pH 7.4 diluent were elevated, and the proportion of female kids was increased. This technology can be used for the reproduction and production of dairy goats in farms at large scales.

On-farm performance evaluation of Maale goats under agro-pastoral management in Southwest Ethiopia

This study was conducted in Maale district Southwest Ethiopia with the objective of evaluating the productive and reproductive performance of Maale goats under agro-pastoral management conditions. Thirty households that have flocks numbers of more than ten were purposely selected and monitored from July 2018 to July 2019. Growth data of birth weight, three month, six month, nine month and yearly weight; birth type and parity were collected. The data were analyzed using the General Linear Model (GLM) procedure of SPSS (21). The overall mean birth weight of the kids was 2.57±0.05 kg (males 2.68±0.07 and females 2.47±0.07 kg). The weaning and six months weight of kids was significantly different (p&lt;0.05) between sexes of kids with 7.66±0.25 and 6.90±0.23 kg for male and female kids, respectively. The average body weight of males and females for six months; nine months and yearly were 11.57±0.30, 10.42±0.28; 15.42±0.27, 14.69±0.25 and 19.03±0.28, 18.43±0.25 kg, respectively. The birth type showed a significant (p&lt;0.05) difference in birth weight. The result further indicated that the weaning weight of single and twin kids was highly significant (p&lt; 0.001) and recorded as 7.66±0.25 and 6.90±0.23 kg, respectively. The overall pre-weaning average daily gain was 51.64±1.89 g day-1 (54.47±2.81 male and 48.93±2.53 g day-1 female). The birth type was significantly (p=0.01) different in pre-weaning growth rate and recorded 57.24±1.63 and 45.24±3.59 g day-1 for single and twin kids, respectively. Twins had higher post-weaning average daily gain than single and recorded as 43.61±1.48 and 40.76 ±0.70 g day-1, respectively. It was concluded that, along with other management interventions, the reproduction and growth performance of Maale goats is reasonably good and suited for community-based breeding strategies and higher performance could be expected. Int. J. Agril. Res. Innov. Tech. 12(2): 7-13, December 2022

Indiscriminate slaughter of pregnant goats for meat in Enugu, Nigeria: Causes, prevalence, implications and ways-out.

The indiscriminate slaughter of pregnant goats (SPGs) undermines meat production and food security especially in developing countries. It also connotes animal cruelty, depletion of goat population and may enhance the spread of zoonotic pathogens inhabiting the female reproductive tract during carcass processing. Consequently, this study determined the causes and prevalence of slaughtering pregnant goats for meat in Enugu, Nigeria. The study also estimated the economic losses associated with SPGs, discussed the negative public health consequences and suggested the ways-out. Structured, validated and pilot-tested questionnaire was used to ascertain the reasons for SPGs for meat among 78 willing and randomly selected respondents. The questionnaire survey was conducted in the form of interview. Pregnancy statuses of the goats slaughtered were ascertained by visual inspection and palpation of the eviscerated and longitudinally incised uteri and the horns for macroscopic evidence of pregnancy. Ages of the dams were estimated by dentition method. Estimation of the gestational age was performed by crown-rump length method. The study lasted for six months, comprised of three months (December to March) during the dry/hot season and another three months (May to August) during the wet/rainy season. Economic loss estimation was based on the current monetary values of a matured (30 kilogram) goat and one kilogram of chevon in Enugu, Nigeria; which was determined through market survey. Pearson's Chi-square test was used to determine whether there were significant (P<0.05) statistical associations between SPGs and age and season. Major reasons adduced for SPGs were: economic hardship (41%), ignorance of the goat's pregnancy status (21%), increased demand for chevon (13%) and feed scarcity during drought (11%). Of the 1,658 does examined during the six months study, 589 (35.5%) were pregnant. The majority (876/1658, 52.8%) of the female goats slaughtered were in their active reproductive age of ≤ 4 years, while 782 (47.2%) were aged > 4 years. Similarly, majority (1007/1658, 60.7%) of the does/nannies were slaughtered during the dry/hot season. A total of 907 foetuses at first (n = 332, 36.6%), second (n = 486, 53.6%) and third (n = 89, 9.8%) trimesters of gestation were recovered from the 589 PGs. Singleton, twin and triplet pregnancies were observed in 312 (53%), 236 (40%) and 41 (7%) PGs, respectively. About ₦34.44 million ($83,390) would have been earned if the foetuses were born alive and raised to maturity. Additionally, 19,136 kg of chevon, valued at ₦47,841, 000 ($115,838), which would have accrued from the wasted foetuses was also lost. Considering the economic, zoonotic and livestock production implications of this work, frantic efforts to reduce SPGs in Enugu, Nigeria is imperative. This could be achieved through advocacy, goat farmers' enlightenment, ante-mortem pregnancy diagnosis, provision of subsidized feed materials during the dry season and strict enforcement of the Nigerian Meat Edict law, which proscribes unapproved slaughter of gravid animals. These measures may improve food safety and security, improve goat reproduction and production, reduce protein malnutrition, limit dissemination of zoonotic pathogens during carcass processing and hence protect public health in Nigeria.

Effect of supplementation of polyunsaturated fatty acid-rich linseed grain on steroid hormones and reproduction in Goats

The effect of polyunsaturated fatty acid (PUFA)- rich linseed grain supplementation was assessed on steroid hormones and reproduction in goats. Twenty-four acyclic and clinically healthy non-descript goats were divided into 4 equal groups. Gr A(control) was supplemented with concentrate without linseed grain while Gr B, C and D were supplemented with concentrate containing 10, 15 and 20% of linseed grain respectively. Goats were supplemented during prebreeding period for 30 days. Blood samples were collected for estimation of serum estradiol and progesterone during pre-breeding and post-oestrus periods. Serum estradiol concentration (pg/ml) was significantly (P<0.05) higher in Gr B (58.79±3.62), C (70.34±5.95) and D (49.48±5.02) compared to control Gr A (38.51±4.03) on 30 days of pre-breeding period. It was significantly (P<0.05) lower in Gr B (9.61±1.45) compared to A (15.61±2.65), C (12.61±1.35) and D (12.61±2.65) on 7 days of post-oestrus period. Serum progesterone levels were comparable among the groups on 30 days of pre- breeding and on 7 days of post- oestrus period. Blood glucose level was comparable on 21 days of supplementation in pre-breeding and 0 day of postoestrus period. Conception (83.33%) and kidding (83.33%) rates were higher in Gr B compared to control Gr A (50.00 and 33.33%). The study suggested that supplementation of PUFA-rich linseed grain in concentrate @ 15% decreased the serum estradiol and increased the serum progesterone during post-oestrus period and improved the conception and kidding rates in goats.

Peripheral neuropathy caused by fenugreek (Trigonella foenum-graecum) straw intoxication in cattle and experimental reproduction in sheep and goats.

Trigonella foenum-graecum (fenugreek) is a legume widely used as a food supplement in humans and less frequently in ruminants. Toxicity has been described sporadically in ruminants grazing mature fenugreek plants or stubble; however, the pathological features are unclear. This report describes a natural outbreak of intoxication in cattle fed fenugreek straw and the experimental reproduction using 8 sheep and 8 goats. Affected cattle presented clinical signs approximately 1 month after consuming the straw and 100 of 400 cattle (25%) were affected, of which 60 of 100 (60%) died or were euthanized. Clinical signs were characterized by proprioceptive positioning defects with abnormal postures and weakness of hindlimbs. Forelimbs were also affected in severely affected animals, and cattle became recumbent. Locomotion was characterized by trembling, and some cattle showed high-stepping movements of their forelimbs and knuckled over in their fetlocks. Experimental intoxication induced clinical signs only in sheep and were similar to cattle, although with signs starting in the forelegs. Gross and microscopic lesions were similar in spontaneous and experimental intoxications. Macroscopic changes corresponded with muscular hemorrhages and edema, mainly surrounding the peripheral nerves. Microscopic examination only demonstrated lesions in the distal peripheral nerves, which included edema, hemorrhages, and Wallerian degeneration. Neurofilament immunohistochemistry revealed altered axon labeling and S100 showed a decrease in myelin intensity and loss of its typical compact arrangement around axons. Biochemical and hematological abnormalities included elevated levels of muscle and liver enzymes and thrombocytopenia. These findings indicate that fenugreek straw induces peripheral neuropathy in cattle and sheep, but not in goats.

Roles of free radicals in reproduction and immune modulations in goats: A review

In the body physiology, gonads (i.e. testes and ovaries) and lymphoid organs are most important tissues in terms of metabolism and dynamicity as functional activities of lymphoid organ and gonad are multistep and energy consuming processes they are always performing a number of biochemical reactions. As a causative effect of the same generation of free radicals are quite obvious. Thus, enormous production of free radicals can limit the proper functional management of lymphoid organ and gonad. We noted significantly high levels of SOD, Catalase, GPx activities and ABTS levels in lymphoid organs and gonads of goats during monsoon and significantly low during winter particularly in lymphoid organs. However, all the parameters were significantly high in gonads during winter only. Malonaldihyde; MDA a marker for lipid peroxidation presented a reverse pattern of activity level than SOD being significantly high during summer but the level was significantly low during monsoon and winter. The glucocorticoid receptor expression was significantly high in spleen and thymus of males during monsoon but the level was significantly high only during monsoon in lymphoid organs of males. All the free radical parameters cumulatively suggest that, monsoon and winter are the seasons of stress for both the sexes of goats as suggested by elevated level of glucocorticoid receptor and thus to counteract the elevated stress level melatonin acted as a “coupler” which not only increased the free radical scavenging enzymes but also scavenged free radicals as an amphipatic free molecules particularly during winter. Winter is the most challenging season for female goats due to “cold stress” as well as “gestational stress” along with energy demanding mega two major events i.e. Maintenance of immunity and gestation is occurring simultaneously.

Comparison of Beetal Goats’ Reproductive Effectiveness under Different Management Approaches

The Beetal goat or Lahori goat is a breed from the Punjab region of Pakistan and India. It is used for milk and meat production. It is similar to the Jamnapari goat and the Malabari goat. Beetal goats have been widely used for improvement of local goats throughout the subcontinent. These goats are also adapted to stall feeding, thus are preferred for intensive goat farming. The Beetal goat and the Malabari goats are usually compared for their body characteristics. The objective of the study was to determine the effectiveness of beetal goat reproduction under different management techniques. Based on reproductive effectiveness and growth parameters, the results of two goat production management systems, namely (1) seasonal breeding and (2) year-round breeding, were compared. The study was performed out during a one-year period. The data was collected from two farms for this study, the 1st farm was the private farm known as Noor-Hussain Goat Farm in Raiwind and 2nd farm was Bahadurnagar Research Institute in Okara which is run by the government. Adult goats (n=72+) were collected for this study and their reproductive and productive traits were examined (2-bucks with each flock).

Effects of N Acetylcysteine on the Expression of Genes Associated with Reproductive Performance in the Goat Uterus during Early Gestation.

Simple SummaryThe uterus is an important place for mammals to nurture new life, and improving the physiological function of the uterus is important for improving the reproductive efficiency of mammals. NAC is a small-molecule antioxidant with a positive regulatory effect on mammalian reproductive performance. We found that NAC can alter the expression of uterine genes in goats in early gestation. These DEGs may regulate uterine performance in early pregnancy in goats by participating in signalling pathways related to reproductive regulation, resistance to oxidative stress, immune regulation, angiogenesis and development, cytokines, and cell adhesion. These findings provide a fundamental reference for the modulation of reproductive performance in goats in early gestation by NAC.N acetylcysteine (NAC) affects antioxidation and reactive oxygen species scavenging in the body and thereby promotes embryonic development and implantation and inhibits inflammation. The mechanism through which NAC regulates reproductive performance in the uteri of goats during early gestation remains unclear. In this study, the treatment group was fed 0.07% NAC for the first 35 days of gestation, whereas the control group received no NAC supplementation. The regulatory genes and key pathways associated with goat reproductive performance under NAC supplementation were identified by RNA-seq. RT–qPCR was used to verify the sequencing results and subsequently construct tissue expression profiles of the relevant genes. RNA-seq identified 19,796 genes coexpressed in the control and treatment groups and 1318 differentially expressed genes (DEGs), including 787 and 531 DEGs enriched in the treatment and control groups, respectively. A GO analysis revealed that the identified genes mapped to pathways such as cell activation, cytokine production, cell mitotic processes, and angiogenesis, and a KEGG enrichment analysis showed that the DEGs were enriched in pathways associated with reproductive regulation, immune regulation, resistance to oxidative stress, and cell adhesion. The RT–qPCR analysis showed that BDNF and CSF-1 were most highly expressed in the uterus, that WIF1 and ESR2 showed low expression in the uterus, and that CTSS, PTX3, and TGFβ-3 were most highly expressed in the oviduct, which indicated that these genes may be directly or indirectly involved in the modulation of reproduction in early-gestation goats. These findings provide fundamental data for the NAC-mediated modulation of the reproductive performance of goats during early gestation.

Reproductive Performance of Kacang Goats in Closed Population Areas of Sidoarjo Regency, East Java, Indonesia

The objective of this study was to determine the reproductive performance of Kacang goats in a closed population area of Oro-Oro Farm located in Sawohan Village, Buduran Subdistrict, Sidoarjo Regency. We used 146 Kacang goat with a range of parity from 1 to &gt; 7. Data analysis was carried out using the ANOVA test, Least Significant Difference (LSD) test, coefficient of variation test and descriptive test to measure litter size, pre-weaning mortality, kidding interval (KI), days open (DO) and Kacang goat reproduction index. The results showed that the highest litter size was 1.69 ± 0.63 at parity 6, the lowest pre-weaning mortality at parity &gt; 7 was 11.76%, the shortest kidding interval and days open was at parity 4 for 7.35 ± 1.22 and 2.35 ± 1.22 months, respectively. The best Kacang goat reproduction index was 2.22 at parity 6 with coefficient of variation of 38.06%, 41.08% and 13.36% for litter size (LS), days open (DO), kidding interval (KI). Conclusively, there was a diverse reproductive performance of Kacang goats in the closed population area of Oro-Oro Farm located in Sawohan Village, Buduran Subdistrict, Sidoarjo Regency based on parity 1 to &gt; 7, in which the higher the parity, the higher the reproductive performance. However, parity 5 onwards saw a declining reproductive performance due to weakened livestock conditions.