Abstract
This study was conducted to determine the polymorphisms of the POU1F1 gene and their relationships with milk yield and components, litter size, birth weight, and weaning weight in goats. For this purpose, a total of 108 Saanen goats from two different farms (Bornova and Manisa) were used as animal materials. Polymorphisms at the exon 6 and the 3 flanking region of the POU1F1 gene were determined by using PCR-RFLP with PstI and AluI restriction enzymes and DNA sequencing analyses. Two alleles and three genotypes were identified by AluI or PstI digestions of the POU1F1 gene. The genotypes frequencies of TT, TC, and CC were 64.8 %, 31.5 % and 3.7 % for the PstI locus; 54.6 %, 31.5 % and 13.9 % for the AluI locus, respectively. T allele frequencies (0.56 and 0.88 for the AluI locus, 0.80 and 0.81 for the PstI locus, respectively) were predominant in both loci at the Bornova and Manisa farms. In terms of POU1F1-AluI and POU1F1-PstI loci, two populations were found to be in Hardy–Weinberg equilibrium. In the POU1F1-AluI locus, significant associations were found between genotypes and lactation milk yield and litter size. Similarly, a significant relationship between genotypes and birth weight in the POU1F1-PstI locus () was determined. The TC and CC genotypes were observed to be higher than the TT genotype for lactation milk yield and litter size at the POU1F1-AluI locus. Birth weight was found to be higher in animals that have the CC genotype at the POU1F1-PstI locus. In conclusion, the POU1F1 gene can be used as a molecular marker for economic features like reproduction, growth, milk content and yield in Saanen goats.
Highlights
Goat populations have increased in recent years in spite of changes in agriculture and technological progress in the world
This study aimed to investigate the polymorphisms of the POU1F1 gene and to evaluate their relationships with some characteristics of reproduction, growth, milk yield and milk components in Saanen goats that are reared inIzmir and Manisa Province
The SNP1 locus at exon 6 had a significant association with hucklebone width (p < 0.05) and the hucklebone width index (p < 0.05) in Guanzhong dairy goats. These results indicate that the POU1F1-AluI locus has significant effects on milk performance and litter size
Summary
Goat populations have increased in recent years in spite of changes in agriculture and technological progress in the world. Demand for goat milk and its products has increased because of an increase in health-conscious consumers. Goat milk provides great advantages to human nutrition such as high digestibility, being antiallergenic, and it has short- and medium-chain length fatty acids (Park et al, 2007; Getaneh et al, 2016). Goat breeding has become popular, and the goat population in Turkey has increased over the last 5 years (FAO, 2016). Goat breeding is carried out in village flocks, plateaus or nomadic flocks in Turkey. In recent years in western Anatolia some entrepreneurs have invested money in intensive farming that provides goat milk to dairy farms.
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