Genetic diversity among Listeria monocytogenes isolates recovered from livestock food products (milk and meat products), vegetables and human clinical cases with bad obstetrics history were studied. A total of 259 samples were screened for isolation, identification and characterization of L. monocytogenes by serotyping PCR and RAPD profiling. In the present investigation, 11.1% prevalence of L. monocytogenes has been recorded. Of these, 5 (19.2%) isolates were from vegetables, 14 (17.5%) from milk, 2 (9.5%) from milk products, 3 (5.7%) from meat, 1 (8.3%) meat products and 4 (5.8%) from humans clinical cases with bad obstetrics history. Serotyping PCR study revealed presence of serotype 1/2b and 4b. RAPD profiling resulted into 5 clusters indicating five major patterns of L. monocytogenes. All clusters included the isolates from various sources. However, cluster 3 and 5 included isolates from human and milk origin, respectively. RAPD pattern 4 and 5 included the isolates belonging to 1/2b or 1/2b and 4b serovar, whereas, other pattern, included L. monocytogenes belonging to various serovars. Prevalence of L. monocytogenes in different food samples signifies its potential as food born pathogen and RAPD-PCR offers greater discrimination of strains.