Abstract
Determination of genetic variation is important to the plant breeders for development of high yielding variety. The aim of the current study was to investigate the genetic diversity of nine tamarind cultivars, out of nine four flowering cultivars using random amplified polymorphic DNA (RAPD) markers. Ten Random amplified polymorphic DNA (RAPD) primers were used to assess the genetic diversity in four flowering cultivars and five non-flowering of tamarind trees. The average genetic similarity level among the four flowering cultivars and five non-flowering accessions grouped into six clusters groups at 0.76%. RAPD profiles of all the tamarind were compared and a total of 58 scorable bands were produced with seven primers ranging from one for OPG-13 to twelve for OPA-R15. Genotypes which were morphological closely related were found to be unrelated at the molecular level. A sizeable amount of intrapopulation diversity recorded in the present study which can be utilized in hybridization programmes to efficiently introgress the desirable trait of interest.SAARC J. Agri., 13(1): 27-36 (2015)
Highlights
Tamarind (Tamaridus indica L.) is a dicotyledonous perennial tree belongs to the family Fabaceae (Leguminaceae) and sub family Caesalpinioideae
Random amplified polymorphic DNA (RAPD) analysis carried out on all the accessions produced a large number of distinct fragments for each primer
Genetic diversity based on RAPD studies of the ten primers used to screen RAPD diversity of T. indica cultivars; seven primers were found to produce distinct polymorphic bands
Summary
Tamarind (Tamaridus indica L.) is a dicotyledonous perennial tree belongs to the family Fabaceae (Leguminaceae) and sub family Caesalpinioideae. It is a diploid species with a chromosome number 2n=26 (Purseglove, 1987). The species has a wide geographical distribution in the subtropics and semiarid tropics and is cultivated in numerous regions. It is mostly self sown or sown with seeds of unknown parentage, which result in wide variation among seedling progenies. Owing to its wide geographical distribution and adaptability to different agro climatic zones, large genetic diversity is present in the seedling population. Due to cross pollination and predomination of seed propagation over a large period of time, it gives immense opportunity to locate elite trees having desirable horticultural traits
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