Abstract
Cadmium, a metal widely used in industrial processes, has been recognized to be a highly toxic and dangerous environmental pollutant. Random amplified polymorphic DNA (RAPD) test is a feasible method to evaluate the toxicity of environmental pollutants on vegetal organisms. Herein, two Iranian ecotypes of Cuminum cyminum (cumin) plantlets following exposure to cadmium (Cd) concentrations of 300–1050 µM for 7 days were screened for DNA genetic alterations by DNA fingerprinting. 10 RAPD primers of 50–70% GC content were found to produce unique polymorphic band profiles on treating cumin seedlings. After Cd treatment, significant changes were observed in RAPD profiles of both ecotypes. These changes included variation in band intensity, disappearance of bands, and appearance of new PCR products in comparison to the control group, and they were dose dependent. These results indicated that genomic template stability (GTS, a qualitative measure reflecting changes in RAPD profiles) was significantly affected at the above Cd concentrations. The GTS index in both ecotypes gradually decreased with an increase in Cd concentrations. These findings suggest that DNA polymorphism detected by RAPD analysis could be a powerful eco-toxicological tool to evaluate the genotoxic effects of cadmium on plants.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
