Abstract
The aim of this study was to apply RAPD technique to analyze the genetic variability among the Iranian CA-MRSA isolates.The RAPD amplification was implemented on 25 strains isolated from the anterior nares of 410 healthy children using four randomly selected oligonucleotide primers from the stocks available in our laboratory, including the primers 1254, GE6, OLP6 and OLP13 from our stock. The amplified PCR products were detected on a 1.5% agarose gel and subjected to further analysis to establish the band profiles and genetic relationships using the Gel Compar® program.The Iranian CA-MRSA isolates produced distinct RAPD patterns which varied based on the primer used, however, the primer 1254 revealed highly polymorphic patterns consisting 5 discernable RAPD types (RT), “RT1” (12, 48%), “RT2” (8, 32%), “RT3” (3, 12%), and “RT4 and RT5”, (a single RAPD type each, 4%). Phylogenetic analysis based on RAPD profiles divided most of the CA-MRSA isolates into 2 distinct but related RAPD clusters, a small group and two single unrelated RAPD types.This study shows that the simple and cost-effective but rather difficult to optimize RAPD fingerprinting could be used to evaluate genetic and epidemiological relationships of CA-MRSA isolates on condition that the patterns are obtained from carefully optimized laboratory tests.
Highlights
Methicillin resistant S. aureus (MRSA) spread is no longer limited to the hospitals, but has emerged in communities to infect an increasing number of people and in particular children (Green et al, 2012)
The Iranian community associated-methicillin resistant S. aureus (CA-MRSA) isolates produced distinct random amplified polymorphic DNA (RAPD) patterns which varied based on the primer used, the primer 1254 revealed highly polymorphic patterns consisting 5 discernable RAPD types (RT), “RT1” (12, 48%), “RT2” (8, 32%), “RT3” (3, 12%), and “RT4 and RT5”
This study shows that the simple and cost-effective but rather difficult to optimize RAPD fingerprinting could be used to evaluate genetic and epidemiological relationships of CA-MRSA isolates on condition that the patterns are obtained from carefully optimized laboratory tests
Summary
Methicillin resistant S. aureus (MRSA) spread is no longer limited to the hospitals, but has emerged in communities to infect an increasing number of people and in particular children (Green et al, 2012). Molecular techniques have been extensively developed for the last 30 years to study the genetic diversity of S. aureus strains and in particular MRSA strains (Mehndiratta & Bhalla, 2012; Vivoni & Moreira, 2005). These techniques have been validated in order to distinguish S. aureus strains for local epidemiologic or outbreak investigation purposes. The molecular approaches such as PCR (Rep-PCR), using primers that target repetitive extragenic palindromic DNA regions (Vecchio et al, 1995), multilocus enzyme electrophoresis (MLEE), a www.ccsenet.org/gjhs
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
