Pure Grade Research Articles

Impurities in commercial titanium dental implants – A mass and optical emission spectrometry elemental analysis

ObjectiveTitanium (Ti) is considered bioinert and is still regarded as the “gold standard” material for dental implants. However, even ‘commercial pure’ Ti will contain minor fractions of elemental impurities. Evidence demonstrating the release of Ti ions and particles from ‘passive’ implant surfaces is increasing and has been attributed to biocorrosion processes which may provoke immunological reactions. However, Ti observed in peri-implant tissues has been shown to be co-located with elements considered impurities in biomedical alloys. Accordingly, this study aimed to quantify the composition of impurities in commercial Ti dental implants. MethodsFifteen commercial titanium dental implant systems were analyzed using inductively coupled plasma-mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES). ResultsThe elemental composition of implants manufactured from commercially pure grades of Ti, Ti-6Al-4V, and the TiZr alloy (Roxolid) conformed to the respective ISO/ASTM standards or manufacturers´ data (TiZr/Roxolid). However, all implants investigated included exogenous metal contaminants including Ni, Cr, Sb, and Nb to a variable extent. Other contaminants detected in a fraction of implants included As and the radionuclides U-238 and Th-232. SignificanceAlthough all Ti implant studies conformed with their standard compositions, potentially allergenic, noxious metals and even radionuclides were detected. Since there are differences in the degree of contamination between the implant systems, a certain impurity fraction seems technically avoidable. The clinical relevance of these findings must be further investigated, and an adaptation of industry standards should be discussed.

Rotational Load Fatigue Performance of Titanium vs Titanium-Zirconium Implant-Abutment Connections.

Titanium-zirconium (Ti-Zr) alloy has been developed to strengthen the implant body, but clinically relevant information is still limited. The aim of this in vitro study was to compare the rotational load fatigue performance of implant-abutment connections in narrow-diameter (3.3-mm) and regular-diameter (4.1-mm) implants made with commercially pure grade 4 titanium alloy (CPTi-G4) and Ti-Zr. Narrow-diameter (N) and regular-diameter (R) implants with CPTi-G4 (Ti) or Ti-Zr (Tz) materials were tested. This resulted in four test groups: NTi, NTz, RTi and RTz. Five specimens were made for each group (n = 5). Abutments used were milled from titanium-aluminum-niobium alloy abutment blanks. A rotational load fatigue machine applied a sinusoidally varying load at an angle of 45 degrees to produce an effective bending moment of 35 Ncm at a frequency of 14 Hz in air at 20°C. The number of cycles to failure was recorded. The upper limit was set as 5 million cycles. Results were evaluated using analysis of variance (ANOVA) and Tukey post hoc tests. Failure locations and patterns were evaluated with scanning electron microscope (SEM). All regular-diameter test groups reached the upper limit of 5 million cycles without failure. All narrow-diameter test groups failed within the range of 402,530 cycles to 3,374,353 cycles. It could be observed that NTz showed a higher mean cycle count as compared to NTi. NTi test group recorded two implants damaged, one implant fracture, five abutment fractures, and four screw fractures. NTz test group showed only abutment fractures at the level of implant platform, with no damage to the implant bodies. Significant difference was found between implants of different diameters. There was no significant difference between implants of different materials. Regular-diameter implants performed significantly better than narrow-diameter implants, regardless of material, while no significant difference in cyclic load to failure was found between groups of different alloys. All NTz failures were at the abutment only, without damage to the implant. This failure pattern can potentially be clinically advantageous in terms of retrieval and subsequent replacement of a failed prosthesis.

Estimated Fatigue Life of Recovery Reclaimed Asphalt Pavement Binder

It is necessary to investigate the physical, chemical characterization and amount of RAP binder in asphalt pavement containing large amount of RAP in order to select suitable pure asphalt binder grade to blending. This research aims to investigate the rheological characteristics and estimation fatigue life of the asphalt binder extraction from two years overlay asphalt pavement. The asphalt was taking from the process of extraction and recovery. Dynamic shear rheometer (DSR) test was conducted on the recovered asphalt to calculate the different variables, complex, elastic and viscous shear modulus, complex viscosity and the phase angle. The main pavement failure modes, rutting and fatigue cracking were addressed by these output parameters of DSR. Fatigue life is related to the energy absorbed during repeated load to pavement layer. The test results indicated that the asphalt extracted from the selected sections were stiff enough to resist rutting and fatigue failure Keywords: Reclaimed asphalt pavement binder, fatigue criteria, VECD, dissipated energy, energy stiffness ratio.

An investigation on the effective parameters and optimal design in ECAP-Conform process of commercially pure titanium using statistical and numerical approaches

The Equal Channel Angular Pressing-Conform process has improved the mechanical and functional properties of materials as well as resolves the disadvantages of conventional ECAP. The main goal of this study was investigation of the variable in ECAP-Conform process of pure titanium Grade 2. The design of experiments with full factorial technique was implemented in conjunction with finite element numerical simulation. The equivalent plastic strain, required torque, applied force on the ECAP die, and the warping radius of the product were measured and results were interpreted using analysis of variance. It was found that the ECAP die angles and the rod bending angle have the highest effect on both imposed strain and required torque. Also, the rod bending angle and the rod-die friction had no significant effect on the warping radius. The optimal values were specified for minimizing the required torque, reaction force, and warping radius, and maximizing the imposed strain. Based on the optimal estimated parameters, the minimum values of torque, force, warping radius, and maximum value of equivalent strain were predicted to 8.4 kN.m, 42 kN, 0.36 m, and 1.7, respectively. Also, the response optimizer obtained the results with less than 8% error in comparison with numerical simulation.

Investigation of an Intensified Thermo-Chemical Experimental Set-Up for Hydrogen Production from Biomass: Gasification Process Integrated to a Portable Purification System—Part II

Biomass gasification is a versatile thermochemical process that can be used for direct energy applications and the production of advanced liquid and gaseous energy carriers. In the present work, the results are presented concerning the H2 production at a high purity grade from biomass feedstocks via steam/oxygen gasification. The data demonstrating such a process chain were collected at an innovative gasification prototype plant coupled to a portable purification system (PPS). The overall integration was designed for gas conditioning and purification to hydrogen. By using almond shells as the biomass feedstock, from a product gas with an average and stable composition of 40%-v H2, 21%-v CO, 35%-v CO2, 2.5%-v CH4, the PPS unit provided a hydrogen stream, with a final concentration of 99.99%-v and a gas yield of 66.4%.

Investigation of Surface Roughness in Incremental Sheet Forming of Conical Drawpieces from Pure Titanium Sheets.

The article presents the results of the analysis of the influence of incremental sheet forming process parameters on surface roughness measured on both sides of conical drawpieces made from pure titanium Grade 2 sheets. The experimental plan was created on the basis of a central composite design. The study assumed the variability of feed rate, spindle speed, and incremental step size in the following range: 500–2000 mm/min, 0–600 rpm, and 0.1–0.5 mm, respectively. Two strategies differing in the direction of the tool rotation in relation to the feed direction were also analysed. Analysis of variance is performed to understand the adequacy of the proposed model and the influence of the input parameters on the specific roughness parameter. The sensitivity of the process parameter on the selected surface roughness parameters was assessed using artificial neural networks. It was found that the change in the surface roughness of the inner surface of the drawpiece is not related to the change of surface roughness of the outer side. The morphology of the outer surface of the draw pieces was uniform with a much greater profile height than the inner surface that had interacted with the tool. Taking into account the outer surface of the drawpiece, the direction of tool rotation is also most closely correlated with the parameters Sa, Sz, and Sku. Step size and feed rate provide the highest information capacity in relation to skewness and kurtosis of the inner surface of the drawpiece.

Recent Advances in Production of Ecofriendly Polylactide (PLA)-Calcium Sulfate (Anhydrite II) Composites: From the Evidence of Filler Stability to the Effects of PLA Matrix and Filling on Key Properties.

The melt–mixing of polylactide (PLA) with micro- and/or nanofillers is a key method used to obtain specific end-use characteristics and improvements of properties. So-called “insoluble” CaSO4 (CS) β-anhydrite II (AII) is a mineral filler recently considered for the industry of polymer composites. First, the study proves that AII made from natural gypsum by a specifically thermal treatment is highly stable compared to other CS forms. Then, PLAs of different isomer purity and molecular weights (for injection molding (IM) and extrusion), have been used to produce “green” composites filled with 20–40 wt.% AII. The composites show good thermal and mechanical properties, accounting for the excellent filler dispersion and stability. The stiffness of composites increases with the amount of filler, whereas their tensile strength is found to be dependent on PLA molecular weights. Interestingly, the impact resistance is improved by adding 20% AII into all investigated PLAs. Due to advanced kinetics of crystallization ascribed to the effects of AII and use of a PLA grade of high L-lactic acid isomer purity, the composites show after IM an impressive degree of crystallinity (DC), i.e., as high as 50%, while their Vicat softening temperature is remarkably increased to 160 °C, which are thermal properties of great interest for applications requiring elevated rigidity and heat resistance.

Chondroitin sulfate: are the purity and the structural features well assessed? A review on the analytical challenges

Animal origin chondroitin sulfate is employed as anti-inflammatory drug and food supplement against anti-osteoarthritis, but also as antioxidant, antitumor, anticoagulant, and immune-regulatory agent or as biomaterial in tissue engineering scaffolds and in drug-delivery systems. As its biological properties depend on the structural characteristics, multi-analytical approaches are necessary to correlate specific features of its heterogenic composition to the different bioactivities. This is of paramount importance to assess the efficacy of pharmaceuticals and food supplements, beyond safety quality control. This review would address the issue of chondroitin sulfate characterization according to the Pharmacopeia testing monograph point of view giving an update of the analytical novelties reported in the last ten years that might be employed for the product testing and releasing on the market. Not-instrumental (e.g. colorimetric assays) and instrumental techniques, most of them coupling diverse chromatographic separation methods with spectroscopic and spectrometry detection techniques, mono and bi-dimensional NMR approaches, are compared as tools to evaluate identity, titer, purity grade, monosaccharide and disaccharide composition, averaged molecular weight and viscosity, charge and sulfate content, impurities and related substances including the presence of other glycosaminoglycans.

Mineral and Isotopic Characterization of Wines From the Douro Demarcated Region

ObjectivesIn the 1960s, the French paradox launched the debate around the benefits of wine consumption. Since then several epidemiological studies have evidenced its potential protective cardiovascular effects. Wine’s characteristics are highly dependent on the terroir. This study aims to characterize the profile of 11 minerals and micronutrients (K, Na, Ca, Fe, P, Mg, Mn, Se, Cu, Cr, and Zn) and the 87Sr/86Sr isotopic ratio, and evaluate suitable tracers of authenticity for the Douro Demarcated Region. It will identify the parameters influenced by processing methods or environmental contamination and the ones common to the region, and fundamentally dependent on the soils and climate. MethodsThe sampling plan comprehended the collection of 15 Port wines and 15 Douro wines. All sample preparation occurred in cleanroom facilities, reagents were of high purity and Ultra-pure water grade I was used. Samples were mineralized using closed vessel microwave digestion. For isotopic analysis, a further purification step was required using Sr-selective resin Eichron®. Minerals and micronutrients were determined by inductively coupled plasma optical emission spectrometry (ICP-OES) and ICP-mass spectrometry (ICP-MS), and isotope ratios by multi collector-ICP-MS (MC-ICP-MS). ANOVA was applied to evaluate significant differences between the two types of wine. ResultsResults are within the maximum levels established by the International Organization of Vine and Wine (OIV), when applicable. k was the most abundant element in both types of wine with values ranging from 399 ± 7 to 1458 ± 7 mg/L. Se was the least abundant one presenting levels between the limit of quantification (2.5 μg/L) and 6.2 ± 0.2 μg/L. The statistical analysis showed significant differences between the two types of wine regarding six elements, namely Ca, Na, Zn, Cu, Cr, and Se. ConclusionsSix elements were identified as potential markers for the wines under study. Since all vineyards under study are grown in the same type of geological formation, the soil should not be responsible for these differences. Instead, they may result from agricultural practices, the winemaking process, or the aging of the wines. Funding SourcesThis work was completed under the METROFOOD-PP project frame and received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 871083.

AUSTRAL WRIGHT TITANIUM GRADE 7 (UNS R52400)

Abstract Austral Wright Titanium Grade 7 (UNS R52400) is a corrosion resistant alpha titanium alloy with mechanical properties similar to those of the commercially pure grades. The presence of 0.12–0.25 % palladium in this alloy improves its corrosion resistance, particularly in mineral acids and strong brines. It is used in applications where the medium is mildly reducing or fluctuates between oxidizing and reducing. This datasheet provides information on composition, physical properties, elasticity, and tensile properties. It also includes information on corrosion resistance as well as forming, heat treating, machining, and joining. Filing Code: Ti-86. Producer or source: Austral Wright Metals.

AUSTRAL WRIGHT TITANIUM GRADE 7 (UNS R52400)

Abstract Austral Wright Titanium Grade 7 (UNS R52400) is a corrosion resistant alpha titanium alloy with mechanical properties similar to those of the commercially pure grades. The presence of 0.12–0.25 % palladium in this alloy improves its corrosion resistance, particularly in mineral acids and strong brines. It is used in applications where the medium is mildly reducing or fluctuates between oxidizing and reducing. This datasheet provides information on composition, physical properties, elasticity, and tensile properties. It also includes information on corrosion resistance as well as forming, heat treating, machining, and joining. Filing Code: Ti-186. Producer or source: Austral Wright Metals.

Fitness accuracy and retentive forces of milled titanium clasp.

Since cast titanium prostheses have many drawbacks, multi-directionally forged titanium grade 2 (MDF) was developed, and the application of the milling process was proposed for improving the titanium clasp. This in vitro study evaluated milled titanium clasps, including MDF titanium. Milling clasps were manufactured with commercially pure (CP) titanium grade 2 (CP 2), grade 4 (CP 4), Ti-6-Al-4V, and MDF. As a control, a CP 2 cast titanium clasp was fabricated in the conventional manner. No porosities and catastrophic failures were observed in the four milled titanium clasps. Fitness accuracy and retentive forces of milled CP 2 and CP 4 tended to be worse, and the milled MDF showed the higher retentive forces (12.45 N) than did cast and milled CP 2 clasps (9.32 N and 4.42 N). Milled titanium clasps can be recommended for longer-term clinical use as compared to cast clasps.

Influence of Electron Beam Welding Parameters on the Microstructure Formation and Mechanical Behaviors of the Ti and Ni Dissimilar Metals Welded Joints

Commercially pure titanium Ti Grade 2, 2 mm in thickness, was welded to 2 mm thick nickel alloy 201 with electron beam welding. Various welding parameters were used to create the butt-welded joints. The innovation herein consists of welding two dissimilar metals that are declared non-weldable. The welding current used for electron beam welding was 40–70 mA and welding speeds were 20–50 mm/s. In this experiment, we tested two offsets of the electron beam, which were 100–300 μm to the nickel side and 200 μm to the titanium side. It was observed that the offset of the beam had no effect on the weld joint’s strength. The samples were subjected to a visual test in which longitudinal and transverse cracks were recorded along the whole weld. Only four samples retained the integrity of the joint. Microstructures of the weld joints were examined by scanning confocal and scanning electron microscopy. Energy dispersive spectroscopy (EDS) analysis confirmed the phase constitution inside the weld regions and the fusion interfaces. Tensile strength and microhardness tests were used to evaluate the mechanical parameters of the Ti/Ni welded joint. The results showed that cracking of brittle Ni–Ti intermetallic phases in electron beam welded joints occurred. The microstructure in the fusion zone’s center part was primarily NiTi and Ti2Ni. No clear correlation was found between heat input or welding parameters—welding current and welding speed—and tensile strength. The strain–tensile strength curve resulted in brittle fracturing. The hardness of the weld zone was five times higher than that of the base metal and heat-affected zone. The amount of heat input into the welded metal is as critical as the large asymmetry in heat transport that controls the process of solidification from each side of the base metal.

Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications.

Zika virus (ZIKV) represents a global human health threat and it is related to severe diseases such as congenital Zika syndrome (CZS) and Guillain-Barré syndrome (GBS). There is no vaccine available nor specific antiviral treatment, so developing sensitive, specific, and low-cost diagnostic tests is necessary. Thus, the objective of this work was to produce the Zika virus envelope protein domain III (ZIKV-EDIII) in Komagataella phaffii KM71H and evaluate its potential for diagnostic applications. After the K. phaffii had been transformed with the pPICZαA-ZIKV-EDIII vector, an SDS-PAGE and Western Blot were performed to characterize the recombinant protein and an ELISA to evaluate the antigenic potential. The results show that ZIKV-EDIII was produced in the expected size, with a good purity grade and yield of 2.58 mg/L. The receiver operating characteristic (ROC) curve showed 90% sensitivity and 87.5% specificity for IgM, and 93.33% sensitivity and 82.76% specificity for IgG. The ZIKV-EDIII protein was efficiently produced in K. phaffi, and it has the potential for diagnostic applications.

A hybrid batch distillation/membrane process for high purification part 2: Removing of heavy impurities from xenon extracted from natural gas

High-purity xenon of 6 N purity grade and higher is widely used in electronics, space, and medicine. A hybrid distillation/membrane process for the purification of xenon extracted from natural gas by gas hydrate crystallization is studied experimentally and via simulations. Membrane gas separation data for xenon-containing mixtures is measured for polysulfone membrane and polyetherimide/polyimide blend membrane. Parametric analysis and process optimization are performed in Aspen Plus Dynamics™ for a middle-vessel batch distillation column with an integrated hollow fiber membrane unit implemented via Aspen Custom Modeler™. Removal of higher- and lower-boiling impurities such as ethane, carbon dioxide, propane, methane, and krypton is considered to achieve 6 N and 9 N target purity grades. Hybrid distillation/membrane process flowsheets built with a multi-stream passive heat exchanger with either retentate or permeate being returned to the column are analysed. It is shown that the operating conditions and the configuration of the hybrid process with a membrane separation treatment of heavy impurities fraction can be tailored to achieve up to 30% increase in batch productivity for the considered case studies and improve xenon recovery reaching above 99.8% as compared to standalone batch distillation, which is especially critical for higher purity grades.

Study on the Mechanism and Oxidative Stress of Compound Danshen on the Degradation of Cx43 and Improvement of Myocardial Apoptosis in ICM Rats.

In order to investigate the mechanism by which Salvia miltiorrhiza degrades connexin 43 (Cx43) and improves myocardial cell apoptosis and oxidative stress in rats with myocardial ischemia (ICM), male SD rats of pure grade are selected (32 rats), adaptively bred for 1 week, and then bred in SPF medium. They are randomly divided into a normal group, a model group, a low-dose Danshen group, and a high-dose group, with 8 rats in each group. HE staining and immunohistochemistry are performed. Serum samples are detected using kits according to instructions, including nitric oxide (NO) and endothelin-1 (ET-1); malondialdehyde (MDA), ELISA for superoxide dismutase (SOD), and vascular cell adhesion molecules (VCAM-1); western blot for detection of cardiac Cx43 protein expression; immunohistochemistry to detect Cx43 expression; and detection of myocardial ischemia. For ICM rats, the application of high-dose compound Salvia miltiorrhiza can effectively prevent the degradation of Cx43 protein, improve the apoptosis of myocardial cells in rats, and have a certain protective effect on ischemic myocardium in rats.

Plant Responses on Elective Gels for in Vitro Shoot Multiplication and Root Elongation of Pineapple (Ananas Comosus L.)

The quest for alternative matrices for plant tissue culture is a continuing process. Agar is one of the utmost common coagulating agents in plant in vitro clonal propagation. Pure grade agar has high value price and fear of over manipulation of its resources affected searching for low cost options. Therefore, liquid medium supplement with silica gel, glass beads and sands substratum and agar in two steps of micro propagation (seedling proliferation and root induction) were investigated. The shoot multiplication in the cytokinins hormone of 2.5mg/l BA and 0.5mg/l KN supported by 7g/l agar and alternative matrices has been produced 33.1 shoots and 5.3 shoot length in sands and agar. There is none significant difference among shoot number and shoot length on the agar and sands on shoot multiplication and plant, and there is significant difference in glass beads and silica gel compared with sand/agar in the shoot multiplication, and there is highly significance in shoot length in the matrices of plant support. Pineapple root induced in auxins hormone in vitro culture of plant propagation in half strength MS media, 30g/l sugar, 1mg/l NAA and 7g/l agar. The higher root number in sands 10.6 and followed 10 in silica gel and agar supported matrices. The root length also none significant difference among means treatments in agar, glass beads and sands. However, there is a significance difference with silica gel in root length. These silica gel, glass beads and sands used as agar gels to support and aeration plantlets. Keywords: Elective gels, low cost, shoots multiplication and root elongation. DOI: 10.7176/JBAH/12-7-03 Publication date: April 30 th 2022

Synthesis and comparisons of Optical and Gamma Radiation shielding properties for ZnO and SiO2 nanoparticles in PMMA nanocomposites thin films

We have used the method of solution casting for producing thin films of PMMA with SiO2 and ZnO composite, with various concentration of the dopant ZnO and SiO2 nanoparticles (NPs) (0.5 wt%, 1 wt% and 2 wt%). In order to prepare the sample, we used a 50µm thick PMMA/ZnO/SiO2 composite thin films (PZSCTFs), while keeping the concentration of SiO2 nanoparticles (NPs) constant at 0.5 wt% the concentration of ZnO (NPs )was varied as 0.5 wt%, 1 wt% and 2 wt% . Further for preparing next specimen just the concentrations of ZnO and SiO2 NPs were reversed. The high peaks of XRD displays the high grade purity of ZnO NPs and amorphous nature of SiO2 NPs. SEM results show that SiO2 is in cluster form and has size below 20nm whereas the ZnO NPs are non-spherical rod like particles of size in range 260nm to 400nm. The outcomes of optical properties shows that the absorption, absorption coefficient, extinction coefficient, reflectance, refractive index, optical conductivity, real and imaginary dielectric constants are directly proportional to the NPs. Whereas the properties like energy bandgap is inversely proportional to the NPs, and for high concentration of ZnO NPs they have a tendency to decrease more. The radiation shielding properties show that PZSCTFs film improved the gamma and UV–radiation shielding ability, are directly proportional to the concentration of NPs and have an greater increasing tendency for high concentration of SiO2 NPs. Dispersion of NPs in PMMA matrix supported to increase the radiation shielding.

Growth of Kaffir Lime (<i>Citrus hystrix</i> DC) Cell Line Derived from Seed Explant After Yeast Elicitation Using Pure and Technical Grade Yeast

The addition of elicitors in kaffir lime (Citrus hystrix DC.) culture is one of strategies for obtaining and increasing the production of secondary metabolites. Saccharomyces cerevisiae is one of the elicitors that can be used to increase secondary metabolites such as terpenoids. However, in its use, the pure cultures of S. cerevisiae are expensive. Therefore, the first objective of this study was to analyze the ability of technical grade (commercial baker’s yeast) to be used as an elicitor and measure the growth of kaffir lime cell line after being elicited by pure and technical grade (commercial baker’s yeast). The second objective is to determine the best time to subculture kaffir lime cell line after elicitation. We observed the morphology and measured the growth curve of pure and technical grade yeast until the 4th subculture generation. Furthermore, we used both grades of yeast for elicitation. Kaffir lime cell suspension was treated with 10 ppm pure grade or 5 ppm and 10 ppm technical grade yeast for 4 days. After elicitation, kaffir lime cell lines were subcultured and their growth was analyzed. The result showed that the morphology and growth curve of technical grade until 4th subculture generations was similar to the pure grade. On the other hand, after elicitation using pure and technical grade yeast and being subcultured, the growth of the elicitated kaffir lime cell line had the same pattern as the control group, but the cell density of the control group was higher than the elicitated group. The initial stationary phase of kaffir lime cell line was on the 17th day which is the best time to subculture. The subculturing process is important to maintain the viability of the kaffir lime cell line.

Superior properties and behaviour of coatings produced on nanostructured titanium by PEO coupled with the EPD process

Surface modification of commercially pure Grade 4 coarse-grained titanium (CG-Ti) and nano-grained titanium (n-Ti) by plasma electrolytic oxidation (PEO) and plasma electrolytic oxidation conjugated with electrophoretic deposition (PEO-EPD) processes is reported in the present study. Two different coatings were developed on each CG-Ti and n-Ti in phosphate-based electrolytes without and with the incorporation of hydroxyapatite (HA) nanoparticles. The phase composition, morphology (surface and cross-sectional), corrosion resistance, surface roughness, and scratch-resistance of the fabricated coatings were thoroughly studied and analysed. The L-929 fibroblast cells were used for assessing the in-vitro cell viability. The L-929 cells cultured on PEO-EPD treated CG-Ti, and n-Ti samples exhibited higher cell growth than PEO treated CG-Ti and n-Ti samples. Among all the PEO and PEO-EPD treated samples, the PEO-EPD treated n-Ti sample showed significantly better corrosion resistance (icorr = 8.85 × 10−7 mA cm−2), lower contact angle (40°), and good adhesion strength (Lc = 29 N), demonstrating the importance of the nanostructuring of the titanium substrate for the properties of the coating. The origin of the discovered enhancement in the properties of the modified PEO coating produced on nanostructured titanium was examined and discussed. After soaking in SBF for 14 days, the PEO-EPD treated sample is wholly covered with apatite layer indicating its good bioactivity