Profile Of Wistar Rats Research Articles

Effects of Intermittent Fasting and Sex on Blood Glucose, Free Fatty Acids, and Lipid Profile in Wistar Rats

Intermittent fasting (IF) has been reported to improve metabolic health through its effect on glucose and lipid profile. However, there is limited information on the effects of Intermittent Fasting on free fatty acids and sex related differences. This study was designed to investigate the effects of Intermittent Fasting (IF) on blood glucose, free fatty acids, lipid profile in both male and female Wistar rats and sex related differences. Twenty Wistar rats of both sexes weighing between 170-200g were used in the study. The animals were grouped into four (4) groups (n=5) per group. Groups 1 and 11 were male and female control groups respectively fed <i>ad libitum</i> with feed and water while groups 111 and 1V were male and female intermittent fasting groups fasted for 16 hours and fed for 8 hours for 28 days respectively. They had free access to water for 24 hours. At the end of 28 days fasting, blood glucose, free fatty acids and lipid profile were determined. The results of the study showed that intermittent fasting was associated with a significant increase in free fatty acids in female rats (p< 0.05) but a non- significant increase in male rats. In both female and male rats, intermittent fasting was associated with a significant decrease (p< 0.05) ins blood glucose, total cholesterol, and low-density lipoprotein, but a non-significant decrease in triglyceride compared with control groups in both male and female rats. In conclusion, Intermittent fasting may help to improve blood glucose and maintain a balanced lipid profile in both males and females. However, Intermittent fasting may also raise the level of free fatty acids and the effect might be more pronounced in females.

Subchronic oral toxicity assessment of Ayurvedic herbo-metallic formulation Mahalaxmi Vilas Rasa in Wistar rats

Abstract BACKGROUND: Mahalaxmi Vilas (MV) Rasa, a revered Ayurvedic herbo-metallic formulation, holds historical significance for its therapeutic potential. In light of its traditional use, a rigorous evaluation of its safety profile in Wistar rats is imperative for establishing its evidence-based safety. This study aims to contribute valuable insights to the scientific community and healthcare practitioners, facilitating informed decision-making regarding the integration of MV into contemporary medical practices. The findings hold relevance for advancing our understanding of Ayurvedic formulations in the context of safety and efficacy, fostering a bridge between traditional wisdom and modern scientific scrutiny. METHODS: In the present study, a total of 24 Wistar rats were randomly divided into four groups as one control vehicle and three dose levels of the test drug MV Rasa. The test drug was made into suspension in a vehicle (honey-to-water ratio, 2:3) and administered orally once a day for 90 consecutive days in three dose levels (51.36, 205.44, and 513.6 mg/kg). Clinical observations, body weight changes, and food and water consumption were noted during the study. Then, on the 91st day, rats were sacrificed humanely, and parameters, such as hematological, biochemical, relative organ weight, and histopathological changes, were studied. RESULTS: There was a significant decrease in water intake in high-dose group rats (513.6mg/kg) in the weeks 1, 3, and 4, as compared with control group rats, the observed effect was not treatment related. In addition, MV (51.36, 205.44, and 513.6 mg/kg) produced no significant alterations in any of the hematological and biochemical parameters, except for a significant increase in direct bilirubin in the moderate dose group (205.44 mg/kg). However, no considerable cellular changes were found in the organs analyzed. CONCLUSION: MV had no toxic effect in Wistar rats during this repeated dose oral toxicity (90 days) study, even at 10 times the therapeutic dose (513.6 mg/kg). This was evidenced by their normal behavioral, hematological, biochemical, and histopathological examinations.

Toxicological Effect of Prolonged Coca-Cola and Bullet Soft Drinks Consumption on Male Hormonal and Testicular Oxidative Profile in Wistar Rats

This study examines the effects of prolonged consumption of soft and bullet drinks on different sex hormones i.e. luteinizing hormones (LH), Follicle Stimulating Hormone (FSH), Testosterone (T), testicular antioxidative parameters ie Malondialdehyde (MDA), superoxide dismutase (SOD)and the histopathological effects of soft drinks consumption on the testis. Thirty five (35) male wistar rats were employed in this study for seven weeks.Group1 (control) was giving normal feeds and water ad libitum, group2 was administered 1.2ml coca cola drinks,Group3 double dose (2.4ml) coca cola drinks,Gruop4 (0.6ml) bullet drinks while 1.2ml sugar solution was given to group5 including normal feeds and water ad libitum.The results from this study shows significant decrease in superoxide dismutase mean values among the test groups compared with control while in Malondialdehyde there was an increased significantly in group4 administered bullet drinks (13.60µg/mg), group2 mean values administered 1.2ml coca cola drinks was (7.50 µg/mg), group3 administered double dose of 2.4ml Coca Cola drinks daily was (6.60µg/mg) while gruop5 mean values administered 1.2ml sugar solution daily was (5.00 µg/mg) compared with the control group of (2.60 µg/mg) mean significant values of <0.05. There was a significant increase in the mean reproductive hormones mean values among the test groups compared with the control group. except the testosterone mean value in group5 administered sugar solution that has lower value than the control group. Findings from this study have shown the toxicological adverse effect of prolonged consumption of coca cola and bullet soft drinks on testicular pathophysiology and significant increase in male reproductive hormones signaling testicular damaged which can result in infertility among males.

Toxicological Effect of Prolonged Coca-Cola and Bullet Soft Drinks Consumption on Male Hormonal and Testicular Oxidative Profile in Wistar Rats

Toxicological Effect of Prolonged Coca-Cola and Bullet Soft Drinks Consumption on Male Hormonal and Testicular Oxidative Profile in Wistar Rats

Long term excessive zinc supplementation in diet induced alteration in serum lipids, hormones and minerals profile in wistar rats and has carry over effect in their F1 generation rats

The use of the zinc in various areas such as animal husbandry, agricultural techniques and multivitamin supplements has increased in recent decades and will increase over time. In this study, the impact of Zn overload for long period in the body, and their carry-over effect on the next generation is determined. Three groups of rats were fed on basal diets having 20 mg Zn in one kg diet (group-1), control group, 50 mg Zn in one kg diet (Group-2) and 80 mg Zn in one kg diet (Group-3) for 180 days. The F1- generation rats obtained from each group of parent rats were fed only on rat pellet diet. The investigation revealed that body weight rise with increased concentration of Zn in parent and F1-generation rats. The blood lipid profile in serum showed an increase in triglycerides, cholesterol, very-low-density lipoprotein (VLDL) cholesterol whereas lowered level of high-density Lipoprotein (HDL) cholesterol levels was recorded in both generation of rats. A significant increase in serum Insulin, HbA1c, and C-reactive protein level was recorded in group-2 and 3 rats while no significant changes in the FI- generation of rats. In F1- rats, Zn showed increase in its level while decreased level of Mn and Cu in the kidney and liver of group-F1-2 and F1-3 was observed in comparison to their control counterparts. The results of the study suggest long term treatment of diet rich in Zn induced alteration in serum lipids, hormones and profile of minerals and has carry over effect to their F1- generations.

Thallium Induced Sub-chronic Toxicity Profile in Wistar Rats

Thallium (Tl) is one of the most toxic heavy metals affecting both humans and animals, yet is one of the least studied for its toxicological effects. A total of 40 male Wistar rats were randomly divided into four Groups with 10 rats in each Group. Group I received distilled water only and served as a control. Group II, III, and IV received thallium sulfate (I) at 0.48, 0.93, and 1.33 mg/kg body weight, respectively, in drinking water for 90 days. In Group IV rats, lethargy, dehydration, weakness, mild diarrhea, weight loss and 100% morality in male Wistar rat were noted. Group II and III rats showed significant reduction in cholesterol, phosphorus, triglyceride level, and absolute and relative spleen weight. Microscopically, thallium sulfate (I) found to cause lymphoid depletion in white pulp of spleen in all treatment Groups. In kidney of Group IV rats, tubular necrosis, cytoplasmic vacuolation or clearing of tubules, cast, and thrombosis were found. Dose dependent hyperkeratosis was found in non-glandular stomach in all three treated Groups. In conclusion, spleen, kidney and stomach were primarily affected organs in sub-chronic thallium toxicity.

Effect of ethanolic leaf extract of Macaranga spinosa Muell-Arg on selected biomarkers of toxicity in Wistar rats

In Nigeria, plant-based herbal medicines are vital component of traditional medicine (TM) and their use for the maintenance of health and wellbeing has been a common practice. These TMs are freely used without mandatory safety or toxicological evaluation. Thus, it is necessary to identify plants with toxicity potentials among the plants used for therapeutic purposes in the country. This study investigated effect of ethanolic leaf extract of Macaranga spinosa Muell-Arg on selected biomarkers of toxicity in Wistar rats. Fifteen Wistar rats of both sexes weighing 96.8 -250 g were divided into three groups comprising of group 1 (control 0.5ml distilled water); group 2 (500 mg/kg b.w Macaranga spinosa Muell-Arg leaf extract) and group 3 (1000 mg/kg b.w Macaranga spinosa Muell-Arg leaf extract). At the end of one week oral administration, animals were sacrificed; blood and tissues samples were taken for biochemical and histopatological investigations respectively. Phytochemicals present were alkaloids, flavonoids, tannins, triterpeneoid/steroids, carbohydrates, cardenolide, and saponins. Urea, sodium ion, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentration were significantly elevated in group 3; non-significant increase in creatinine, potassium, bicarbonate, chloride ion and alkaline phosphatase (ALP) concentration in group 2 and 3 compared to group 1. Total protein and triglyceride were significantly decreased in group 3 compared to group 1. Albumin, total and conjugated bilirubin were non-significantly decreased in group 2 and 3 when compared to control group 1. Histological examination of kidney tissue showed no change in kidney histology; liver tissue indicated vacuolar and cytoplasm degeneration, and fatty change. Hart tissues showed normal cardiac myocytes. This study has established that ethanolic leaf extract of Macaranga spinosa Muell-Arg can cause hepatoxicity, mild nephrotoxicity and no significant alteration of lipid profile in Wistar rats. Therefore, users of this plant in TM should avoid excessive and prolong use, as it can be toxic to the liver.

Effects of “Reed A Dream” Insecticide on Selected Biomarkers of Kidney, Liver, and Lipid Profile in Wistar Rats

This study investigated the effects of “Reed A Dream” (RD) insecticide on selected biomarkers of kidney, liver, and lipid profile in Wistar rats. Twenty-five (25) rats of both sexes (weighing 50-114 g) were divided into 5 groups, comprising of control (0.5mL water), N-hexane only (0.5mL), RD(50% vol/vol) + N-hexane, RD(25% vol/vol) + N-hexane, and RD (12.5% vol/vol) + N-hexane, also labelled as groups 1-5 respectively. After 2 weeks of oral daily administration, the animals were sacrificed; blood, liver, kidney, and heart tissues were taken for biochemical and histopathological investigations. A non-significant decreased (p≥0.05) in plasma concentration of total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in groups 3-5, a significant decrease (p≤0.05) in albumin (groups 3-5); a non-significant increased in creatinine, gamma glutamyltranspeptidase (GGT) in groups 3-4 was observed, as compared with the control group; Moreover, a significant increased (p≤ 0.05) in urea in groups 3-5 when compared with the control group. Histology of the liver tissue (Group 3) revealed a central vein with mild congestion, while kidney tissues (groups 3-5) showed visible congestion of glomerulus, and heart tissue (group 3-5) showed interstitial deposition of collagens, as compared with the control group. RD insecticide caused acute nephrotoxicity and mild hepatotoxicity in Wistar rats on oral exposure.

Honey supplemented with Vitamin C prevents dyslipidaemia and oxidative stress induced by exposure to lead acetate in Wistar rats

Objectives: Lead is an environmental toxicant known to cause dyslipidaemia through oxidative stress damage. The therapeutic potential of honey has widely been reported. However, there is a paucity of reports on its effects on lipid profile in Wistar rats exposed to lead. Materials and Methods: The effects of honey on antioxidants and lipid profile indicators were studied in 25 male Wistar rats. The rats were randomly assigned to one of the five groups (n = 5) as follows: Group I served as the control and received deionised water; Group II served as a lead acetate group (40 mg/kg bw) and Groups III, IV and V served as lead acetate groups coadministered with honey (1 mL/kg bw), Vitamin C (100 mg/kg bw) and honey+Vitamin C, respectively. The treatments were orally administered for 28 days. Body and liver weights were determined using an analytical weighing balance. Glucose and lead concentration, superoxide dismutase (SOD), total antioxidant capacity (TAC), malondialdehyde (MDA), and the lipid profile indicators were determined using spectrophotometry. The liver histology was assessed by haematoxylin and eosin staining techniques. Statistical analysis was done using analysis of variance, and the results were expressed as mean ± S.E.M. at P < 0.05. Results: Body weight, SOD, and TAC increased significantly in the treatment groups compared to lead acetate only. However, lead, glucose concentration, MDA, total cholesterol, triglycerides, HDL, and LDL decreased significantly in the treatment groups compared to lead acetate only. Normal histoarchitecture of the liver was seen in the treatment groups compared to lead acetate, which showed areas of inflammation. Conclusion: These findings imply that honey prevents dyslipidaemia which is a risk factor for metabolic diseases.

Synergistic Effect of Ethanolic Extract of Phyllantus Muellerianus and Eremomastax Speciosa Leaves on Lipid Profile of Wistar Rat

The study investigated the synergistic effect of ethanolic extract of Eremomastax speciosa and Phyllantus muellerianusleaves on the lipid profile of Wistar rats. The Wistar rats were assigned into two groups of 10 animals each; the control was given normal rat pellet and water, while the experimental group was given the mixture of the ethanolic plant extracts at 200mg/kg body weight, by orogastric intubations once daily. The animals were fed for 3weeks (21 days) during which anthropometric measurement and data were collected. Twelve hours after the feeding and administration of both plants extract, the animals were anesthetized under chloroform vapor and then dissected. Blood samples were collected from the heart by cardiac puncture into plain tubes and taken to the laboratory for analysis. The phytochemical result indicates that the extract contains a high amount of flavoniods and alkaloids; a moderate amount of saponins, tannins, and polyphenols with a mild amount of glycosides. The result of the study shows a mean significant decrease in the TC level and LDL-C level; an abnormal increase in the levels of TG, VLDL-C, and an abnormal decrease in HDL-C between the experimental and control group at a probability level of p<0.05. The phytochemical result indicates that the plant can be used to manage many health conditions like Stomach troubles, headaches, jaundice, and anemia (Obenet al., 2006). While the overall result of the study reveals that the administration of both extracts has both hypolipidemic effects and hyperlipidemic effects on the lipid profile levels correlate with cardiovascular disease.

Duration of exposure dependent effect of carbamate treated net on serum lipid profile of Wistar rats

The duration of exposure dependent effect of Carbamate treated net on serum lipid profile of female Wistar rats was investigated in this study. Eighteen (18) animals were randomly divided into three (3) study groups with six (6) rats each. Group 1 was designated as the control group while groups 2 and 3 constituted the experimental groups containing rats exposed to Carbamate treated net for 30 and 60 days respectively. The rats were exposed daily to Carbamate treated net by whole body and nose inhalation mode of exposure. The body weight of rats was measured at the commencement of the experiment and at the end of the experiment. The organ weight result obtained indicated that the body weight increased significantly in the rats of the experimental groups when compared to the rats in the control group at p<0.05. The result obtained indicated that the body weight increased significantly in the rats of the experimental groups when compared to the rats in the control group at p<0.05. The total cholesterol (TC), triacylglycerol (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) concentrations in the sera of the rats were determined at the end of the experiment. The result obtained showed that the TC, TG, HDL-C and LDL-C levels increased significantly in the rats of the experimental groups when compared to the rats in the control group at p<0.05. The observation from this study suggests that long-term exposure to Carbamate treated net in household setting may alter the level of the serum lipid profile of the experimental animals, thereby leading to various health complications.

Evaluation of heart rate variability, blood pressure and lipid profile alterations from dual transceiver mobile phone radiation exposure

Electromagnetic fields have been reported to alter electrical activities in the brain and heart. However, there is paucity of information on the potential functional alterations that magnetic fields from mobile phone could cause to the heart. This study investigated heart rate variability (HRV), blood pressure (BP) and lipid profile in Wistar rats exposed to electromagnetic field radiation from a dual transceiver mobile phone (DTrMP). Twenty-one male albino Wistar rats (140-180g) were randomly assigned to two major groups positioned 5m apart as follows: control: no phone (n=7) and treatment group (n=14) continuously exposed to electromagnetic field from Tecno T312 DTrMP 900/1800MHz set in silence mode. Experimental treatment consisted in 10min calls/day, directed to this device for a period of six weeks. Seven animals from the treatment group were allowed to recover for a period of two weeks after exposure. HRV, systolic, diastolic and mean arterial BP were noninvasively investigated, while serum lipid profile and heart tissue nitric oxide (NO) activities were determined using standard procedures. There was significant (p<0.05) increase in systolic, diastolic, mean arterial BP and a decrease in HRV. Serum high density lipoproteins decreased, while total cholesterol, atherogenic indices, and heart NO levels increased significantly in the radiation exposed animals. The alterations observed in exposed animals remained unchanged even after the recovery period. These results suggest that exposure to electromagnetic radiation from dual transceiver mobile phones could be a risk factor to increase in blood pressure.

THE HEMATOLOGIC PROFILE IN THE ACUTE TOXICITY TEST OF COGON GRASS ROOTS ETHANOL EXTRACT IN WISTAR RATS

Objective: This study aimed to investigate the hematologic profile of Wistar rats in the acute toxicity test of Cogon grass roots ethanol extract (CGEE).&#x0D; Methods: Cogon grass roots were dissolved in 70% ethanol. An acute toxicity test was conducted based on The National Agency of Drug and Food Control of the Republic of Indonesia. Five female rats in the treatment group were administered a single high dose of 5000 mg/kg body weight (BW) of CGEE in 200 μl of 0.5% carboxymethyl cellulose (CMC), and the 5 female rats in the control group were administered 200 μl of 0.5% CMC. After 14 d, blood samples were collected, and 18 hematologic parameters were measured with a hematology analyzer. Statistical analyses were performed to compare the parameters between the two groups with the independent t-test for normally distributed data and the Mann Whitney test for non-normally distributed data.&#x0D; Results: None of the hematologic parameters in the treatment group significantly differed from those in the control group after 14 d of observation (P&gt;0.05).&#x0D; Conclusion: A single high dose of 5000 mg/kg BW of CGEE did not change the hematologic profile of Wistar rats. These results indicate that CGEE does not have an acute hemotoxic effect, at least for hematologic parameters.

Formulation and in vitro / in vivo Evaluation of Novel Biodegradable Microspheres for Treatment of Hormone Responsive Cancers

Objectives: The present work relates with developing long acting controlled release poly(DL-lactide-co-glycolic acid) (PLGA) microspheres of Goserelin acetate for treatment of breast and prostate cancer. Methods: Microspheres were prepared by using biodegradable polymers PLGA 50:50 and PLGA 75:25 employing modified emulsification method by using static mixer. Evaluate the effect of process and formulation variables on the characteristics of microspheres were studied. Particle size distribution, surface morphology, encapsulation efficiency, in vitro drug release and in vivo drug release profile in Wistar rats were investigated. Results: Preparative variables such as concentrations of stabilizer, drug-polymer ratio, stirring rate and ratio of internal to external phases were found to be important factors for the preparation of Goserelin acetate loaded PLGA microspheres. These changes were also reflect in vitro and in vivo drug release profiles. The result indicated that the morphology of Goserelin acetate PLGA microspheres presented as spherical shape with smooth surface, homogenous drug distribution and the particle size distributed from 88.11 to 116.78 μm. In vitro and in vivo release profile showed controlled initial burst release followed by one month sustained release profile of the Goserelin acetate loaded PLGA microspheres. Conclusion: The process and formulation variables could be effectively modified to achieve the desired characteristics and one month sustained release of Goserelin acetate loaded biodegradable polymeric microspheres showed potential for treatment of hormone responsive cancers.

The Effect of Plectranthus amboinicus Lour Spreng Ethanolic Extract on Relative Organ, Body Weights Changes, and Hematology Profile in Wistar Rats Treated with 7,12Dimethylbenz(a)anthracene

This study aims to examine the protective properties of ethanol extract Plectranthus amboinicus (EEP) leaves on body weight gain, the relative weight of liver, kidney, lung, spleen, thymus and haematological profile of rats induced by 7,12Dimethylbenz(a)anthracene (DMBA). Used 25 female rats, divided into five groups namely NC (given Carboxymethyl cellulose (CMC) 1%), PC (given DMBA 20 mg/kg body weight), T1, T2, and T3 were each given DMBA 20 mg/kg body weight once every four days for 32 days and EEP 175, 350, and 700 mg/kg body weight given every day from day 33 to 59. Both the DMBA and EEP are given orally using a gastric gavage. On day 60, rats were killed by neck dislocation, bood collected in EDTA tubes for hematological analysis, rats are dissected to obtain liver, kidney, lung, thymus, and spleen organs. Data were analyzed with one way Analisys of varians (ANOVA). The results of this study indicate that EEP in T1, T2 and T3 treatments has no effect on weight loss compared to PC. There was no effect of EEP on the relative weight of the liver, kidneys, spleen, thymus and lungs. EEP increased the number of erythrocytes, leukocytes and platelets in rats that had DMBA

Capsinoids Supplementation does not Prevent Weight Gain and does not Change Lipid Profile in Wistar Rats Fed a High-Fat Diet

We hypothesized that capsinoids supplementation, a bioactive vanillylamines isolated from chili peppers, would exert protective effects against high-fat diet -induced weight gain via regulation of lipid, glucose and insulin profile. To test our hypothesis, twenty-four male Wistar rats were fed a standard diet, standard diet with capsinoids, high-fat diet and high-fat diet with capsinoids for 6 weeks. Capsinoids dose was 0.18 mg/kg/d. During the experiment, body weight and food intake were evaluated weekly and biochemical analyses were performed at the end of the experimental period. Only high-fat diet with capsinoids presented lower total food intake as compared to standard diet with capsinoids group (~35%). Nevertheless, there were no significant differences in weight gain between the groups. Capsinoids supplementation did not prevent retroperitoneal and epididymal fat gain on high-fat diet with capsinoids, as well did not change brown fat and liver weight. Moreover, no statistical difference was observed for high-fat diet or capsinoids supplementation on blood glucose, insulin and lipid profile. In conclusion, these results suggest that capsinoids supplementation in obesity experimental model of Wistar rats, has limited effects to prevent weight and fat gain, as well as do not regulate metabolic profile.

Frutanos tipo inulina associados à dieta de cafeteria: efeito no perfil cardiometabólico de ratos Wistar

A dieta de cafeteria foi desenvolvida a fim de mimetizar a dieta ocidentalizada, uma vez que esta associa-se a desordens no perfil cardiovascular, como dislipidemias, hiperglicemia, acúmulo de gordura corporal, entre outros. Os frutanos tipo inulina (FTI) são fibras solúveis categorizados como prebióticos. O presente estudo objetivou avaliar os efeitos da dieta de cafeteria associadas aos FTI sobre o perfil cardiometabólico em ratos Wistar. Para tanto, utilizou-se 30 animais. Os FTI purificados, bem como, provenientes da matriz alimentar (yacon em pó), foram adicionados a seus respectivos grupos experimentais na dose de 6%, durante 30 dias. As variáveis analisadas foram: composição centesimal e conteúdo de FTI, ganho de peso (GP), peso relativo do ceco (PRC), peso relativo do fígado (PRF), consumo alimentar, glicemia, triglicerídeos, colesterol total, HDL-c, LDL-c, tecido adiposo subcutâneo e visceral (epididimal, retroperitoneal e mesentérica). Todas as análises seguiram os protocolos padronizados. A análise estatística foi realizada no software Sigma Stat 4.0 e teste de normalidade de Shapiro-Wilk, testes descritivos, ANOVA ou teste de Kruskal-Wallis seguidos dos testes de comparação de médias de Duncan ou Dunn’s. O nível de significância adotado foi de p&lt;0,05. A variável PRC foi estatisticamente maior nos grupos submetidos aos FTI. O grupo que recebeu dieta de cafeteria apresentou valores superiores no GP, VLDL, triglicerídeos, tecido adiposo subcutâneo e epididimal. Não houve diferença estatística nos parâmetros PRF, colesterol total, LDL-c, HDL-c e glicemia. Portanto, através deste trabalho, verificou-se efeitos sistêmicos benéficos dos FTI em parâmetros como: PRC, GP, VLDL, triglicerídeos, tecido adiposo.

Effects of Methanolic Root Extract of Holarrhena floribunda on the Lipid Profile and Sex Hormones in Wistar Rats

Aim: This study aims to determine the effects of methanolic root extract of Holarrehna floribunda on the level of some serum sex hormones, follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, estradiol, progesterone and lipid profile in Wistar rats. &#x0D; Materials and Methods: Twenty- four male and female Wistar rats (150-250 g body weight) were randomly assigned into 4 groups of 6 rats each. Group 1 (control male) took normal rat chow and drinking water. Group 2 (control female) took normal rat chow and drinking water, Group 3(Male test group), was administered with 200 mg/kg of Holarrehna floribunda extract, Group 4(Female test group), was administered with 200 mg/kg of Holarrehna floribunda. The feeding regimens lasted for 5weeks.&#x0D; Results: The values for the lipid profile shows CHOL in Group 1 and Group 3 were 4.02±0.41 mmol/l and 5.75±0.09 mmol/l, Group 2 and Group 4 were 3.87±0.22 mmol/l and 5.80±0.10 mmol/l respectively, TRIG for Group 1 and Group 3 were 1.30±0.05 mmol/l and 2.11±0.15 mmol/l for Groups 2 and 4 were 1.15±0.03 mmol/l and (2.10±0.12 mmol/l,HDL-C for Groups 1 and 3 were 30.01±0.82 mg/dL and 39.88± 1.24mg/dL,for Groups 2 and 4 were 29.98± 0.77 mg/dL and 38.20± 1.83 mg/dL, LDL-C for Groups 1 and 3 were 2.26±0.37 mmol/L and 4.17±0.35 mmol/L respectively, for Groups 2 and 4 were 3.27±0.47 mmol/L and 4.06±0.21 mmol/L respectively, shows significant increase(p&lt;0.05) in HDL-C (mg/dL) in Groups 1,2,3 and 4.The values obtained showed significant increase (p&lt;0.05) in HDL-C (mg/dL) in Groups 1,2,3and 4The serum concentrations of FSH, Estradiol, LH and progesterone in the control Group 2 were 0.38 ±0.07mIU/mL, 7.83±0.35 mIU/mL, 15.50±0.15 Pg/mL and 0.86±0.03 ng/mL respectively, and in test Group 4 were 0.77±0.04mIU/mL, 8.75±0.17 mIU/mL, 21.09±0.79 Pg/Ml 0.33±0.05 ng/mL. Follicle stimulating hormone levels was significantly higher (p&lt;0.05) in test group compared with control. While in Group 1 and 3, the serum concentrations of FSH, LH, and testosterone in control Group 1 were (0.51 ±0.06) mIU/mL, (16.44±0.31) mIU/mL and (8.41±0.50) ng/mL respectively and in test Group 3 were 0.88±0.06 mIU/mL19.88±1.46mIU/mL and10.68±0.64 ng/mL, respectively.&#x0D; Conclusion: The extract improves the level of sex hormones in both the male and female rats. which could enhance reproductive functions in normal rats and those with loss of reproductive function.

EFEITO BIOLÓGICO DO EXTRATO DE FOLHAS DE OLIVEIRA (OLEA EUROPAEA L.) EM RATOS SUBMETIDOS À DIETA HIPERLIPÍDICA

Objetivo: A fim de explorar as características nutricionais e bioativas da folha de oliveira, este estudo teve como objetivo avaliar o potencial biológico das folhas de oliveira, analisando o efeito do extrato das folhas no perfil glicêmico e lipídico de ratos da linhagem Wistar submetidos à dieta hiperlipídica. Metodologia: O experimento teve duração de 70 dias, onde os animais foram divididos e distribuídos aleatoriamente em 4 grupos de 6 ratos cada, sendo N: dieta normocalórica + água; H: dieta hiperlipídica + água; NE: dieta normocalórica + extrato; e HE: dieta hiperlipídica + extrato. Ao final do experimento foi determinado o efeito do extrato aquoso das folhas de oliveira na glicemia, perfil lipídico e na peroxidação lipídica dos ratos. Resultados: O consumo do extrato de folhas de oliveira pelos ratos promoveu alterações no perfil lipídico e efeito benéfico em relação aos níveis de TBARS. Conclusões: Devido a sua composição química rica em nutrientes e compostos com propriedades bioativas, além de suas consideráveis atividades antioxidante e biológica, as folhas de oliveira, podem ser uma opção como suplemento alimentar com atividade biológica.

PERFIL LIPÍDICO E GLICÊMICO DE RATOS SUBMETIDOS A DIETAS COM FIBRA DA CASCA E TALO DE BANANA

Objetivo: O estudo teve por objetivo avaliar os efeitos da ingestão de dietas contendo a fibra presente na casca e talo da banana, no perfil glicêmico e lipídico de ratos da linhagem Wistar. Metodologia: Estudou-se, durante 65 dias, a influência de dietas contendo fibras provenientes da casca (GFC), talo da banana (GFT) e ambas as fibras (GFCT), em relação à dieta controle (GA), no perfil glicêmico e lipídico de 24 ratos machos da linhagem Wistar, com peso médio inicial de 54,06g, distribuídos de forma equitativa em quatro grupos. O grupo controle (GA) recebeu dieta AIN acrescida de 1% de colesterol cristalino e 0,1% de ácido cólico, e as dietas experimentais foram semelhantes ao grupo controle, com substituição da fonte de fibra. Resultados: Nos grupos de tratamento (GFC, GFT e GFCT), houve aumento dos níveis glicêmicos, de triglicerídeos e redução do colesterol total e LDL-C séricos, em relação ao grupo controle (GA). Os níveis de VLDL-C mostraram-se reduzidos apenas para o grupo contendo fibras provenientes da casca. Conclusões: A inserção de fibra na dieta, proveniente da casca e talo da banana, bem como da mistura de ambos, mostrou efetividade de uso sobre o perfil lipídico e capacidade de manutenção dos níveis de glicemia dos ratos alimentados com dietas contendo fibra oriunda da casca e do talo.DOI: 10.12957/demetra.2018.29559 DOI: 10.12957/demetra.2018.29559