Preparation Of Zinc Oxide Nanoparticles Research Articles

Preparation of Zinc Oxide Nanoparticles and the Evaluation of their Antibacterial Effects.

Nosocomial bacterial infections have become increasingly challenging due to their inherent resistance to antibiotics. The emergence of multidrug-resistant bacterial strains in hospitals has been attributed to the extensive and varied use of antibiotics, further exacerbating the problem of antibiotic resistance. Metal nanomaterials have been widely studied as an alternative solution for eradicating antibiotic-resistant bacterial cells. Metallic nanoparticles attack bacterial cells through various mechanisms, such as the release of antibacterial ions, generation of reactive oxygen species, or physical disruption, against which bacteria cannot develop resistance. Among the actively researched antimicrobial metal nanoparticles, zinc oxide nanoparticles, which are FDA-approved, are known for their biocompatibility and antibacterial properties. In this study, we focused on successfully developing a precipitation method for synthesizing zinc oxide nanoparticles, analyzing the properties of these nanoparticles, and conducting antimicrobial tests. Zinc oxide nanoparticles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS), ultraviolet/visible spectroscopy, and X-ray diffraction (XRD). Antibacterial tests were conducted using the broth microdilution test with the multidrug-resistant strains of methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. This study demonstrated the potential of zinc oxide nanoparticles in inhibiting the proliferation of antibiotic-resistant bacteria.

Preparation of zinc oxide nanoparticles using Citrus sinensis fruit peel aqueous extract and its antimicrobial study

Preparation of zinc oxide nanoparticles using Citrus sinensis fruit peel aqueous extract and its antimicrobial study

Colchicine-capped ZnO nanoparticles: Elucidation of their cytotoxicity potential against MDA-MB 231 cancer cells

This research descibes a pioneering approach aimed at preparing zinc oxide nanoparticles (ZnO-NPs) with colchicine as the reducing and capping agent. Colchicine-loaded ZnO-NPs (CHZnO-NPs) were prepared by adding colchicine to the zinc sulfate heptahydrate solution. The CHZnO-NPs formulation was then characterized to determine the morphology, size, crystallinity, elemental composition and vibrational properties. Upon characterization, CHZnO-NPs were studied for their cytotoxic effect against the breast cancer cell line (MDA-MB-231). The successful biosynthesis of CHZnO-NPs was initially confirmed visually by the changes in the mixture color, from light-yellow to white cloudy. The best CHZnO-NPs formulation selected was F3, which possessed 10 mg/mL of colchicine. Formulation (F3) had the smallest mean particle diameter of 43.77 nm and the lowest zeta potential of −19.60 mV. It also had 92.21 ± 0.012 % encapsulation efficiency and 20.86 ± 0.005 % drug loading. Formulation (F3) displayed a hexagonal wurtzite structure with irregular morphology. The observation of colchicine peaks on the FTIR spectra of F3 proved the role of colchicine as a reducing and capping agent during the synthesis of ZnO-NPs. Besides, the in-vitro cell cytotoxicity study on the MDA-MB-231 cell line revealed a significant reduction in cell proliferation at the concentration of 25 μg of colchicine and F3. Further, studies on the cellular migration potential also demonstrated concentration-dependent activity. Overall, CHZnO-NPs were shown to be successfully synthesized via an environmental-friendly procedure and colchicine acted as a capping agent to regulate the particle size, and aggregation, in addition to its anticancer properties.

Antibacterial and photocatalytic effects of newly synthesized zinc oxide nanoparticles derived from Mongolian honey

Development of bio-compatible, bio-safe and environmentally friendly nanoparticles is a matter of urgency for research in the field of nanotechnology. In this study, we aimed to prepare zinc oxide nanoparticles from Mongolian honey as raw material and to determine its biological activities. Honey-based zinc oxide nanoparticles were obtained by green synthesis method, and their characteristics and biological activities were evaluated. Developed zinc oxide nanoparticles from Khentii honey and Selenge honey were at a size of 16.02 nm and 95.23 nm, respectively. A characteristic band of Khentii honey-based zinc oxide nanoparticles was observed at 466 cm–1 and a band of Selenge honey-based zinc oxide nanoparticles was also observed at 434 cm–1. Antibacterial and photocatalytic effects were detected for the developed nanoparticles. The study suggested that newly synthesized honey-based zinc oxide nanoparticles might be an effective tool against bacterial infection.

Fast and efficient removal of metronidazole from aqueous solution using graphene oxide (GO) supported nitrogen (N) doped zinc oxide (ZnO) nanoparticles

This work focused on the preparation of ZnO nanoparticles through modifications by nitrogen incorporation (N-ZnO NPs) and thereafter immobilized them on graphene oxide (GO) nanosheets to form GO-N-ZnO nanocomposites, followed by its application for the removal of metronidazole (MNZ) antibiotic from aqueous solution by effective adsorption and visible light driven photocatalysis. The structural, functional, surface morphology and optical properties of the synthesized samples were investigated by numerous techniques. In the comparative study of catalytic degradation of MNZ, GO-N-ZnO demonstrates 96.6% removal efficiency from aqueous solution by adsorption and photocatalysis under visible light irradiation and corresponding degradation kinetics have been studied. Thereafter, effects of initial concentration of MNZ and GO-N-ZnO catalyst dosages have been investigated. Scavengers test reveals that superoxide radicals are responsible for such fast improved degradation. A possible explanation is provided for such fast improved degradation of MNZ by GO-N-ZnO catalyst. The reusability and stability tests demonstrate that as prepared GO-N-ZnO can be used up to fourth consecutive cycles with satisfactory removal efficiency (88.4%) and retains its crystal structure. Finally, microbiological assay showed that the degraded MNZ aqueous solution has diminished capacity to inhibit bacterial growth compared to the control MNZ solution. These results describe the potential of environmental-friendly GO-N-ZnO catalyst for remediation and long-term reusability.

Green route to prepare zinc oxide nanoparticles using Moringa oleifera leaf extracts and their structural, optical and impedance spectral properties

This article investigates biosynthesis of zinc oxide (ZnO) nanoparticles (NPs) from Moringa oleifera leaves extract using an eco-friendly preparation method. The crystalline structure, optical properties, morphology and impedance characteristics of ZnO NPs were analyzed using impedance spectroscopy, powder X-ray diffraction (XRD), scanning electron microscopy, Fourier transform infrared spectroscopy (FTIR) and ultraviolet spectroscopy (UV-vis). The powder XRD pattern confirmed the crystallinity of the prepared samples as well as enabled determining their crystallite size and pure phase portion. The FTIR study confirmed the presence of functional groups responsible for reduction metal ions into ZnO NPs. UV-vis absorption spectra contained the absorption peak corresponding to ZnO NPs. Impedance spectroscopy of the prepared ZnO NPs revealed the grain boundaries in them and confirmed their semiconducting nature.

Plant phytochemicals-mediated synthesis of zinc oxide nanoparticles with antimicrobial, pharmacological, and environmental applications.

Nanotechnology is a fast-growing field with large number of applications. Therefore, the current study, was designed to prepare Zinc Oxide nanoparticles (ZnO NPs) from A. modesta leaves extract through a cost-effective method. The prepared NPs were characterized through UV-Vis Spectroscopy (UV-Vis), Dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD), scanning electron microscope (SEM), and energy dispersive X-ray (EDX). The XRD and DLS analysis revealed the hexagonal nanocrystalline nature of ZnO NPs. The FTIR results displayed multiple fictional groups and UV results confirmed its optical properties. The average size of the NPs was 68.3 nm with a band gap of 2.71 eV. The SEM images divulge a clover leaf shape of ZnO NPs. The EDX spectrum revealed the presence of zinc and oxygen. The prepared NPs showed excellent biomedical application. The highest antileishmanial activity was 68%, anti-inflammatory activity was 78%, total antioxidant capacity (TAC) was 79.1%, antibacterial potential (ZOI) 22.1 mm, and highest growth inhibition of 85 ± 2.1% against A. rabiei. The adsorption efficiency of 85.3% within 120 min was obtained. Conclusively ZnO NPs have shown potential biomedical and environmental applications and ought to be the more investigated to enhance their practical use.

ELECTROCHEMICAL PERFORMANCE OF ZINC OXIDE NANOPARTICLES PREPARED VIA GREEN SYNTHESIS ROUTE USING CHROMOLAENA ODORATA LEAVES EXTRACT AS POTENTIAL ANODE MATERIAL IN SODIUM-ION BATTERY

A paradigm shift in the preparation of nanoparticles from a chemical synthetic route to the green synthetic method using plant extractives has gained much impetus. This study used Chromolaena odorata leaf extract to prepare zinc oxide nanoparticles (ZnO-NPs). The ZnO-NPs were characterised by spectroscopic, optical and thermal techniques. Thermogravimetric Analysis (TGA) revealed no significant weight loss observed after 550°C for precursor powder which was further calcined using a furnace at 800°C and 900°C to obtain ZnO-NPs. Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy analysis indicated Zn-O stretching between 400 cm-1 to 500 cm-1. UV-Vis spectra show a strong absorption band at 388 nm for both calcination temperatures. Scanning Electron Microscopy (SEM) images showed the irregular shapes of the samples at different calcinations. The X-ray diffraction (XRD) diffractograms showed that the synthesised ZnO-NPs are in a hexagonal wurtzite structure. Studies of the electrochemical performance showed that ZnO-NPs made with Chromolaena odorata extract and calcined at 800oC have better cyclability than ZnO-NPs calcined at 900oC. Due to better cyclability, these ZnO-NPs can be applied as anode material in sodium-ion batteries.

Fabrication, Characterization and In Vitro Cell Cytotoxicity of ZnO Nanoparticles on MDA‐MB 231 Breast Cancer Cell Line

AbstractThe intention of this study was to prepare zinc oxide nanoparticles (ZnO‐NPs) via bottom‐up approach and evaluate their physiochemical, antimicrobial and anticancer properties. ZnO‐NPs were first prepared by the direct precipitation method with zinc sulphate heptahydrate, followed by characterization tests using sophisticated analytical and imaging instruments. Upon characterization, ZnO‐NPs were studied for their antimicrobial activities against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa as well as their anticancer activity against breast cancer cell line (MDA‐MB 231). The ZnO‐NPs possessed a mean particle diameter of 225.2 nm and a zeta potential of −15.8 mV. A sharp absorption peak of ZnO was recorded at 354 nm via UV‐Visible spectroscopy. Hexagonal wurtzite shape of ZnO‐NPs has been observed and confirmed by Scanning Electron Microscopy (SEM) and X‐Ray diffraction (XRD). ZnO‐NPs exhibited excellent antimicrobial activity against the Gram‐positive bacteria, Staphylococcus aureus. In an in vitro cell cytotoxicity study, ZnO‐NPs showed a dose‐dependent relationship against MDA‐MB 231 breast cancer cells. It was observed that as the concentration of ZnO‐NPs increased (0.25 μg to 35 μg), there was a sharp reduction in the proliferation rate of the cancer cell line. Overall, our study reported successful synthesis of ZnO‐NPs, which can be efficient antibacterial and anticancer agents.

In vitro Seed Germination Studies in Barley Exposed to Zinc Oxide Nanoparticles

Background: Nanotechnology is an emerging field that could lead to novel applications in the fields of biotechnology and agriculture. The present study was conducted to test the efficacy of zinc oxide (ZnO) nanoparticles on the germination of barley seeds. Methods: The current study used a chemical method to prepare ZnO nanoparticles. An absorption band at 322nm using UV/Visible spectroscopy was obtained due to the formation of ZnO nanoparticles. The seeds were given 100, 200, and 500 ppm of ZnO nanoparticles. Seeds in the control group were given distilled water. Zinc was also given as zinc nitrate (0.1M) to the seeds of the zinc- treated group. Results: ZnO nanoparticles at higher concentrations were found to be toxic to plants, whereas their lower concentrations boosted the yield and growth of the plant. The present study showed that ZnO nanoparticles have a significant impact on the seed germination potential and could provide an alternative source for fertiliser or growth enhancers that may improve sustainable agriculture.

Preparation of dual-use GPTES@ZnO photocatalyst from waste warm filter cake and evaluation of its synergic photocatalytic degradation for air-water purification

Organic pollutants are the most critical threats to the health of air and water resources. On this basis, fabricating a photocatalytic acrylic film with dual-use (i.e. removing benzene from air and MB/MO dyes from water) was aimed in this research. For this purpose, waste warm filter cake (WWFC) was used to extract zinc from it. Zinc element was separated from WWFC by a basic leaching method and acidified to prepare zinc oxide nanoparticles. In the following, a simple hydrothermal method was used to increase the surface functionality of the extracted ZnO nanoparticles in order to establish active reaction sites for reaction to silane coupling agent and increase in the holes that were prepared during photo-excitation. Thereafter, the nanoparticles were modified with 3-glycidoxypropyltriethoxysilane (GPTES) at different concentrations. The band gap of the modified nanoparticles decreased from 3.25 to 3.1 eV by surface modification. The photocatalytic performance of ZnO nanoparticles was assessed by degradation of MB and MO aqueous solution (50 ppm) under simulated UV/Visible irradiations. MB and MO were degraded 91 and 60% under UV light and 65 and 50% under visible light after 150 min of irradiation. The photo degradation rate increased after adding carboxy methyl cellulose (CMC) surfactant to methylene blue and adding cocamide-dea (CDE-G) surfactant to methyl orange. The results confirmed that the green surfactants improve the dispersion and surface interaction of the modified nanoparticles in the dyes solution and cause more electron charge transfer which creates effective photocatalytic sites. The prepared nanocomposite films were placed in a photo-reactor to remove gaseous benzene from air under UV/visible irradiation. Gas chromatography (GC) results showed that the modified nanoparticles removed up to 35.25 and 20.34% of benzene from air. Colorimetric analysis (ΔE*) showed that the acrylic film contained modified nanoparticles degraded 91 and 82% of MB, and 85 and 76% of MO under UV/visible lights, respectively. In the end, it can be said that these photocatalytic films are able to remove environmental pollution in air and water.

Plant mediated green synthesis of zinc oxide nanoparticles using Citrus jambhiri lushi leaves extract for photodegradation of methylene blue dye

Due to the discharge of organic and inorganic contaminants into the water, the textile industry is the main cause of water pollution. Nanoparticles have been synthesized in recent years to filter out such textile pollutants from water. In the current study, an efficient and facile method, i.e., the green method, has been used to prepare zinc oxide nanoparticles (ZnO NPs) by utilizing Citrus jambhiri lushi leaves extract. The morphological, optical, and structural properties were investigated by scanning electron microscopy, UV–Vis spectroscopy and x-ray diffraction technique. Results affirmed that ZnO NPs possessed the bandgap and particle size in the range of 3.00–3.05 eV and 10–35 nm, correspondingly. Furthermore, ZnO NPs demonstrated effective photodegradation of methylene blue dye in just 120 min, making them an excellent photocatalyst for removing textile pollutants from water.

Biosynthesized ZnO-NPs Using Sea Cucumber (Holothuria impatiens): Antimicrobial Potential, Insecticidal Activity and In Vivo Toxicity in Nile Tilapia Fish, Oreochromis niloticus

In this study, a sustainable and eco-friendly method was used to prepare zinc oxide nanoparticles (ZnO-NPs) using a sea cucumber aqueous extract. Then, ZnO-NPs were characterized by instrumental analysis (UV-vis, HR-TEM, XRD, FT-IR, and DLS) and evaluated for their possible antibacterial, antifungal, and insecticidal activities. Additionally, the toxicity of ZnO-NPs was evaluated in vivo against Nile Tilapia (Oreochromis niloticus). The sea cucumber was collected from the Gulf of Suez (Red Sea) at Al-Ain Al-Sokhna coast in Egypt and identified as Holothuria impatiens. The prepared Hi-ZnO-NPs peaked at 350 nm in UV–Vis spectral analysis. They showed quasi-spherical shaped particles with sizes ranging from 13 nm to 47 nm and a predominate size of 26 nm as indicated by HR-TEM. The XRD pattern of Hi-ZnO-NPs revealed a crystalline phase with an average size of 17.2 nm as calculated by Debye–Scherrer equation. FTIR analysis revealed the possible role of H. impatiens biological molecules in the biosynthesis process of ZnO-NPs. Hi-ZnO-NPs showed a negative zeta potential of −19.6 mV, demonstrating moderate stability. Biosynthesized Hi-ZnO-NPs revealed broad antimicrobial activity against Gram-positive bacteria (S. aureus ATCC 25923 and E. feacalis), Gram-negative bacteria (S. typhi, K. pneumonia and E. coli), and filamentous fungi (Aspergillus niger). Hi-ZnO-NPs demonstrated larvicidal activity against the mosquito, Culex pipiens (LC50 = 2.756 ppm and LC90 = 9.294 ppm), and adulticidal action against the housefly, Musca domestica (LD50 = 4.285 ppm and LD90 = 22.847 ppm). Interestingly, Hi-ZnO-NPs did not show mortality effects against Nile tilapia fish (Oreochromis niloticus), highlighting the potential safety of Hi-ZnO-NPs to highly exposed, non-target organisms. However, histopathological and hematological investigations provided dose-dependent impacts of Hi-ZnO-NPs to Nile tilapia. Overall, data provide an eco-friendly approach for synthesizing novel Hi-ZnO-NPs with multiple biomedical properties and potentially low toxicity to Nile tilapia fish.

Preparation of Zinc Oxide Nanoparticles Assisted by Okra Mucilage and Evaluation of its Biological Activities.

In this study, zinc oxide nanoparticles (ZnO-NPs) were biologically synthesized from Abelmoschus esculentus L. (Okra) mucilage fraction (OM). Analytical techniques were employed to study the formation and properties of OM-ZnO NPs, including their morphology, shape, size distribution, and surface charges. Additionally, OM-ZnO NPs were assessed for their antimicrobial, antioxidant, and cytotoxic properties. UV-visible spectroscopy confirmed the formation of OM-ZnO NPs, evident by the appearance of an SPR peak at 368.8 nm. The FTIR spectroscopy demonstrated that OM functional groups contribute to the formation and stability of the NPs. Micrographs from TEM and SEM showed that OM-ZnO NPs ranged from 15-40 nm in diameter, whereas hydrodynamic diameter and surface charge values obtained from Zeta and DLS were 72.8 nm and 14.6 mv, respectively. XRD analysis indicated the OM-ZnO NPs were crystalline with a wurtzite structure and a crystallite size of 27.3 nm, while EDX revealed a zinc: oxygen ratio of 67.5:34. Further, the OM-ZnO NPs demonstrated significant antimicrobial activity in response to different types of bacteria. In the antioxidant assay, the OM-ZnO NPs scavenged DPPH with 68.6% of the efficiency of ascorbic acid (100%). The present study demonstrated the cytotoxic efficacy of MO-ZnO NPs against MCF7 cells with an IC50 of 43.99 μg/ml. Overall, the green synthesis of ZnO NPs by OM was successful for many biological applications, such as antimicrobial, antioxidant, and anticancer. Moreover, OM-ZnO NPs can be applied as a biologically-derived nanotherapeutic agent.

Development of novel green synthesized Zinc oxide nanoparticles with antibacterial activity and effect on diabetic wound healing process of excisional skin wounds in nursing care during sports training

The current study proposed a green, eco-friendly approach for the preparation of zinc oxide nanoparticles using leaf extract of Nigella sativa plant extract. The synthesized ZnO NPs were characterized using techniques such as FESEM, EDS, UV–Vis, FTIR and XRD. The findings obtained from DLS and FESEM revealed the formation of polydisperse ZnO NPs with an average particle size of 45 nm. Further, antidiabetic studies revealed that the treatment of diabetic mice with ZnO NPs revealed the recovery of glycogen, insulin and blood glucose levels. In addition, the present results also revealed the potential characteristics of fabricated ZnO NPs with better efficacy and low cost than the alternative treatments for diabetes. Further, this work opened the possibilities for future studies, primarily with respect to the development of new drugs for use in diabetic wound care during sports training.

Photocatalytic degradation of hazardous Rhodamine B dye using sol-gel mediated ultrasonic hydrothermal synthesized of ZnO nanoparticles

The present study is focused on the sol-gel ultrasonic hydrothermal synthesis of zinc oxide (ZnO) nanoparticles and its application in the degradation of Rhodamine B (RhB) dye. ZnO nanoparticles were synthesized with varying temperatures at 90 °C, 190 °C and 550 °C. Zinc nitrate hexahydrate [Zn(NO3)2·6H2O] and potassium hydroxide were used to prepare ZnO nanoparticles and investigated using the X-ray diffraction (XRD), field emission scanning electron microscope (FESEM) and diffuse reflectance spectroscopy (DRS) for crystallinity, surface morphology, and band gap, respectively. Results obtained from XRD analysis shows that the synthesized ZnO nanoparticles are of hexagonal structure and crystalline in size ranged from the 21.1–32.41 nm. The effects of temperature show that the crystalline size of ZnO increased with increasing temperature and surface morphology investigated by FESEM. DRS used to calculate the band gap of nanoparticles shows that 3.26 eV at 90 °C, 3.25 eV at 190 °C and 3.23 eV at 550 °C. The optimal condition was determined using the response surface methodology (RSM) based Box Behnken design (BBD) method. We found that the ZnO material synthesized at 90 °C exhibits a smaller size, providing a larger surface area for photocatalytic degradation of the RhB dye. Energy is saved as it was synthesized at low temperatures. The maximum removal efficiency of Rohdamine B (RhB) dye (25 mg/L) obtained were 95% at optimized conditions i.e. pH 7, 2 g/L catalyst dose after 70 min of ultraviolet photocatalytic treatment.

Anticancer effect of zinc oxide nanoparticles prepared by varying entry time of ion carriers against A431 skin cancer cells in vitro.

Although, zinc oxide nanoparticles (ZRTs) as an anti-cancer agent have been the subject of numerous studies, none of the reports has investigated the impact of the reaction entry time of ion-carriers on the preparation of ZRTs. Therefore, we synthesized variants of ZRTs by extending the entry time of NaOH (that acts as a carrier of hydroxyl ions) in the reaction mixture. The anti-proliferative action, morphological changes, reactive oxygen species (ROS) production, and nuclear apoptosis of ZRTs on human A431 skin carcinoma cells were observed. The samples revealed crystallinity and purity by X-ray diffraction (XRD). Scanning electron microscopy (SEM) images of ZRT-1 (5min ion carrier entry) and ZRT-2 (10min ion carrier entry) revealed microtubule like morphology. On prolonging the entry time for ion carrier (NaOH) introduction in the reaction mixture, a relative ascent in the aspect ratio was seen. The typical ZnO band with a slight shift in the absorption maxima was evident with UV-visible spectroscopy. Both ZRT-1 and ZRT-2 exhibited non-toxic behavior as evident by RBC lysis assay. Additionally, ZRT-2 showed better anti-cancer potential against A431 cells as seen by MTT assay, ROS generation and chromatin condensation analyses. At 25μM of ZRT-2, 5.56% cells were viable in MTT test, ROS production was enhanced to 166.71%, while 33.0% of apoptotic cells were observed. The IC50 for ZRT-2 was slightly lower (6μM) than that for ZRT-1 (8μM) against A431 cells. In conclusion, this paper presents a modest, economical procedure to generate ZRT nano-structures exhibiting strong cytotoxicity against the A431 cell line, indicating that ZRTs may have application in combating cancer.

Statistical physics modeling and evaluation of adsorption properties of chitosan-zinc oxide nanocomposites for the removal of an anionic dye

The development of rapid methods to synthesize cost-effective adsorbents for the dye removal is an emerging and relevant topic in the field of wastewater purification. This study focuses on the preparation of zinc-oxide nanoparticles (NPs) impregnated chitosan (CS@ZnO) nanocomposite beads via mechanical stirring (MS) and microwave-assisted (MA) routes. The beads were characterized and utilized for the removal of Eriochrome Black-T (EBT) dye. Results showed that the CS@ZnO-MA beads provided the best adsorption capacities for EBT removal (40.9 ± 0.83 mg g−1) at pH 3.5. The best composite (CS@ZnO-MA) can be regenerated 5 times without significantly affecting its adsorption properties and can remove 71 % of EBT dye from a multi-pollutant solution. The novelty of this study relies on the facile preparation and impregnation of the biopolymer with metal-oxide NPs, which render high adsorption capacity, mechanical strength and high specific surface area of the CS to contribute in the removal of dye molecules from textile wastewater. An enhanced statistical physics model revealed that the adsorption mechanism of EBT dye molecules on these composites was multimolecular. The study indicates an effective synthesis route that can be implemented to prepare new adsorbents using CS at a large-scale for water treatment.

A novel green preparation of zinc oxide nanoparticles with Hibiscus sabdariffa L.: photocatalytic performance, evaluation of antioxidant and antibacterial activity

ABSTRACT This study investigates the eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) utilizing an aqueous solution of Hibiscus sabdariffa L. flower extract, which is acts as reducing agent as well as capping agent. The Fourier transform infrared spectroscopy (FTIR) results revealed the presence of flavonoids and phenols in the plant extract, indicating that they were the major agents capable of reducing zinc nitrate salt. According to our x-ray diffraction (XRD) results, ZnO-NPs exhibit a particular phase wurtzite structure. The ZnO-NPs are spherical in shape and have an average size of 15 nm, according to the measurements of electron microscope (SEM) and transmission electron microscope (TEM) measurements. Energy dispersion (EDX) analysis demonstrates that the NPs are mainly composed of zinc and oxygen. The zeta potential of these nanoparticles shows that they are very stable. The antibacterial activity of ZnO-NPs was tested using agar dilutions with a variety of gram-positive and gram-negative microorganisms. According to the research results, ZnO-NPs can be established as an extremely specific antibacterial agent for a wide variety of organisms to prevent bacterial growth. Furthermore, the antioxidant properties of ZnO-NPs were determined using the 2,2 diphenyl-1-picrylhydrazyl hydrate (DPPH) radical scavenging approach, and the IC50 value of 38 μg/mL was measured for ZnO-NPs. Furthermore, the biosynthesized ZnO-NPs showed significant catalytic performance of methyl orange (MO) under UV irradiation. Overall, ZnO-NPs in their produced state have excellent potential in biomedical and wastewater treatment applications. Radical scavengers were used to evaluate the role of radicals in the reaction mechanism.

Facile green preparation of zinc oxide nanoparticles and its effects on in vitro feed degradation, ruminal fermentation, and total gas production.

Facile green preparation of zinc oxide nanoparticles and its effects on in vitro feed degradation, ruminal fermentation, and total gas production.