In vitro Seed Germination Studies in Barley Exposed to Zinc Oxide Nanoparticles

Abstract

Background: Nanotechnology is an emerging field that could lead to novel applications in the fields of biotechnology and agriculture. The present study was conducted to test the efficacy of zinc oxide (ZnO) nanoparticles on the germination of barley seeds. Methods: The current study used a chemical method to prepare ZnO nanoparticles. An absorption band at 322nm using UV/Visible spectroscopy was obtained due to the formation of ZnO nanoparticles. The seeds were given 100, 200, and 500 ppm of ZnO nanoparticles. Seeds in the control group were given distilled water. Zinc was also given as zinc nitrate (0.1M) to the seeds of the zinc- treated group. Results: ZnO nanoparticles at higher concentrations were found to be toxic to plants, whereas their lower concentrations boosted the yield and growth of the plant. The present study showed that ZnO nanoparticles have a significant impact on the seed germination potential and could provide an alternative source for fertiliser or growth enhancers that may improve sustainable agriculture.