Precision Resolution Research Articles

Dopamine-integrated all-hydrogel multi-electrode arrays for neural activity recording.

Investigation of brain neural circuits is essential for deciphering the diagnostics and therapeutics of neurodegenerative diseases. The main concerns with traditional rigid metal electrodes include intrinsic mechanical mismatch between sensing electrodes and tissues, unavoidable foreign body responses, and inadequate spatiotemporal resolution, resulting in a deficiency of sensing performance. All-hydrogel neural electrodes with multi-electrode arrays (MEAs) suggest a viable way to modulate the trade-off between tissue-mechanical compliance and excellent spatiotemporal recording capacity, but still face the issues of insufficient conductivity and unstable interlayer bonding. Herein, we constructed a four-layer all-hydrogel neural electrode, by sandwiching a conductive hydrogel layer within two encapsulation hydrogel layers, with a shielding hydrogel layer located on top. We introduce a dual-strategy treatment to induce controllable phase separation in poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) hydrogel, which achieved ultra-high conductivity (up to 4176 S cm-1) comparable to that of metals and precise spatial resolution (∼15 μm) suitable for single neuron recording. In addition, the utilization of polyphenol chemistry mediated adaptive adhesion endowed this neural electrode with flexible and stable interlayer bonding among conductive-encapsulation-shielding layers and the tissue-electrode interface. Consequently, the all-hydrogel neural electrode exhibited a tenfold higher signal-to-noise ratio than a commercial silver electrode, realized the recording of weak neural activity signals within single and multiple neurons in epileptic rats, and applied man-made stimulation to the cerebral cortex of rats during seizures. This work provides a useful tool to understand the development, function and treatment of neurodegenerative diseases.

Development and Validation of Reverse-Phase High-Performance Liquid Chromatography Method for Simultaneous Estimation of Doxorubicin and Clotrimazole.

A reverse-phase high-performance liquid chromatographic (RP-HPLC) method was developed to analyze the simultaneous estimation of doxorubicin and clotrimazole. The method was achieved by Nucleodur C18 column with dimension 250 × 4.6 mm (5 μm) using gradient elution. The mobile phase contained 0.2% formic acid (pH 3.2) and acetonitrile. The flow rate was kept at 1.0 mL/min and detection and quantitation of both drugs (doxorubicin and clotrimazole) were achieved using a photodiode array detector at 276 nm, which was the isosbestic point for both drugs. The proposed method was validated according to the current International Council for Harmonization of Technical Requirements of Pharmaceuticals for Human Use guidelines for specificity, linearity, accuracy, precision, and robustness. The developed method showed a linear response (R2 > 0.999), and was accurate (recoveries 97%-103%), precise (resolution ≤1.0%), sensitive, and specific. Thus, the developed RP-HPLC method for the simultaneous estimation of both drugs was successfully validated and can be utilized for the estimation of these drugs in the formulations being developed.

Potential of Knudsen Effusion Mass Spectrometry (KEMS) for Energy Materials Science

The determination of valuable thermodynamic data for materials at elevated temperatures is a very important issue in Materials Science. This can be achieved by the elucidation of vaporization processes with mass spectrometric techniques.Knudsen Effusion Mass Spectrometry provides the identification of gaseous species present above condensed phases and permits the determination of their partial pressures up to a total pressure of 200 Pa.The key for establishing model calculations using DG minimization tools like among others FactSage, Pandat or Thermocalc is the knowledge of the thermodynamic data of molecules and compounds. This includes the partial pressures of gaseous species, the standard enthalpies and entropies of formation of gaseous and condensed phases as well as their enthalpies and entropies of mixing and the knowledge of phases and chemical compounds which are formed upon reactions.The method will be described and examples and an insight will be given how basic thermodynamic data are derived with the above mentioned method for the incorporation into thermodynamic databases. Examples of investigations carried out in our lab will be given.The number of groups worldwide using this advanced technology has been shrinking during the last decades. Therefore, we are pleased to announce the setup of a completely new designed instrument in our laboratory which can determine data with higher precision and better resolution.The new set up will be presented as well.

In-Operando Detection Method for Lithium Metal Deposition during Fast Charging of Lithium-Ion Batteries Derived from Impedance Modeling

Lithium-ion batteries play a key role in the electrification of the transport sector. However, to replace combustion engines with battery-powered electric drive trains, the fast charging capability is essential. Therefore, it is necessary to identify the optimum operating window for lithium-ion cells. A strict criterion for optimization is to prevent lithium metal deposition at the anode, as this undesired side reaction can pose serious safety risks. An efficient technique to optimize charging strategies while tracking lithium plating is needed, as existing approaches have limitations such as a lack of in-line capability, low time precision, or complex experimental setups. In this study, a simple lithium metal deposition detection method is derived from a three-electrode setup. This in-operando method is applied during fast charging using dynamic electrochemical impedance spectroscopy. To model the acquired impedance spectra, a novel equivalent circuit is developed. The model is used to elucidate characteristic impedance features of anodes with deposited lithium metal. These observed characteristic features are then applied to a simplified measurement method in full cells using a single-frequency signal. The precision and high temporal resolution of both methods are demonstrated through validation. Furthermore, the simplified method can be applied in in-operando applications, making it suitable for diverse research and industrial contexts.

Synthesis of signal formation and processing blocks of diffuse light reflection sensor using FPGA PSoC5 microcontroller

The problem of partial implementing the functionality of the electronic blocks for forming and processing the diffuse light reflection sensor's signal using an FPGA-microcontroller PSoC5 is solved. The topicality of this problem is caused by necessity of precision and high resolution measurement of the difference between, on the one hand, the reference signal of light reflection from the clear sensor's working surface and, on the other hand, the signal of diffuse light reflection from the inspected corroded surface. For this purpose, in particular, a channels' multiplexer, measuring and reference operational amplifiers of the second stage, a delta-sigma ADC with differential inputs, as well as a PWM control and synchronization scheme are implemented on the built into microcontroller FPGA consisting of universal digital blocks (UDB) together with programmed logical devices (PLD). The only components implemented outside the FPGA-microcontroller are the first stage operational amplifiers (in amount of sixteen), the current pulses generator for LED, as well as power supply block. The combination of components synthesized on FPGA with a powerful 32-bit Arm Cortex-M3 core makes it possible to ensure flexibility of settings, to minimize the influence of dark currents of sensitive elements of the sensor's photodiode linear array as well as noises, to align programmatically the sensitivity of the measuring channels and to carry out sensor calibration. Thus, noise mitigation is implemented programmatically by ADC's data collection and averaging in measurement buffer or calibration buffer (for reference signal from clear working surface). Several software routines for mode selection and settings, channels scanning (including measurement and calibration with alignment), as well as for measurement data (difference between the measurement and calibration buffers) displaying and transfer to PC (via one of onboard communication devices) have been developed. These all features can allow implementing the algorithms for deep analysis of measurement data and for calculation of the characteristics of surface corrosion defects.

Energy correlators on tracks: resummation and non-perturbative effects

Energy correlators measured inside high-energy jets at hadron colliders have recently been demonstrated to provide a new window into both perturbative and non-perturbative Quantum Chromodynamics. A number of the most interesting features of these correlators, namely their universal scaling behavior and the ability to image the confinement transition, require precise angular resolution, necessitating the use of tracking information in experimental measurements. Theoretically, tracking information can be incorporated into the energy correlators using track functions, which are non-perturbative functions describing the fragmentation of quarks and gluons into charged hadrons. In this paper, we apply our recently developed track function formalism to energy correlators, and study in detail the interplay of track functions with perturbative resummation and non-perturbative power corrections. We provide resummed results for the energy correlators at collinear next-to-leading-logarithmic accuracy and compare with parton shower Monte Carlo simulations. For the two-point correlator the use of tracking has a minimal effect throughout the entire distribution, but it has a significant effect for higher point correlators. Our results are crucial for the theoretical interpretation of recent experimental measurements of the energy-energy correlators.

Present-day crustal movement and seismic moment balance on major active faults around the easternmost segment of the Kunlun fault, Tibetan Plateau, China

After the 2021 Ms 7.4 Madoi, China earthquake located south of the eastern Kunlun fault, the coseismic Coulomb stress changes show that the earthquake potential is enhanced towards the easternmost segment of the Kunlun fault. Before the 2008 Mw 7.9 Wenchuan, China earthquake, the easternmost segment of the Kunlun fault is considered to being a region with the highest frequency occurrence of moderate-to-strong earthquakes in mainland China, where there are still at least three seismic gaps at present, indicating an urgent earthquake hazard. The tectonic deformation pattern is closely related to the development and occurrence of earthquakes. In this study, we collect all the available GPS data to obtain a crustal movement velocity field with high precision and high resolution by removing nontectonic effects within the study area. The GPS velocity profile and two-dimensional strain rates are then derived from the updated GPS velocities to analysis the characteristics of crustal deformation. Our results demonstrate that the Longriba fault zone slips predominantly right-laterally with the rate of 5.2 ± 0.5 mm/yr and the crustal shortening rate is ∼0.5 mm/yr across the Longriba and Longmenshan faults. Along with notable crustal extension and contraction, the principal strain rate field is distributed in a wide area on the northwest part of the Longmenshan fault, and decreases southwestward along the Longmenshan fault zone. In addition, we provide the time series of 8 campaign GPS stations which have never been published before. Moreover, the moment deficit estimate is a well-established way of assessing the earthquake hazard at a specific fault. Accompanying with the inverted slip rates and locking depth, we estimate the rates of moment accumulation on major faults and compare them with the seismic moment released on each fault derived from the Chinese historical earthquake catalog which extends for more than 2000 years in many regions. We find that the moment deficit is mainly concentrated on the Longriba fault with a value of 4.18 × 1017 Nm/yr. The Longriba fault have accumulated enough seismic energy to generate one Mw > 7.7 earthquake on it, or two Mw > 7.5 earthquakes on its two branches in the future. This study is an essential indicator for understanding the present-day tectonic deformation pattern in the Tibetan Plateau and is also helpful for seismic hazard assessment.

Terahertz spin currents resolved with nanometer spatial resolution

The development of coherent terahertz (THz) spin currents with femtosecond temporal resolution has been extensively studied due to its significant implications for advancing high-speed information processing devices. However, the precise spatial resolution of THz spin currents, which is crucial for increasing storage density, is still unknown. In this study, we employ spintronic THz emission nanoscopy (STEN) to achieve efficient injection and accurate detection of femtosecond THz spin currents with nanoscale lateral spatial resolution (∼60 nm). The occurrence of emission signals at the fifth harmonic order indicates a substantial signal-to-noise ratio. Additionally, STEN proves to be an effective method for characterizing and etching nanoscale spintronic heterostructures. The integration of nanophotonics, nanospintronics, and THz-nanotechnology into a unified platform is poised to enable the characterization of spin states at micro-to-nanoscale densities, accelerate the development of high-frequency spintronic optoelectronic nanodevices, and catalyze other revolutionary technical applications.

Neuronal hyperexcitability in AD

AbstractBackgroundBecause neuronal and network deficits are the imperative immediate consequences of pathological changes of disease mechanisms, neurophysiological manifestations associated with Aβ and tau are uniquely positioned to demonstrate the abnormal homeostatic and maladaptive circuit alterations and the dynamic integrated effects of underlying genetics and neuropathological changes in Alzheimer’s disease (AD). Importantly, network dysfunction manifesting as abnormal hyper‐ as well as hypo‐active oscillatory brain rhythms is detectable even during the prodromal phase of disease. Here we leveraged the higher spatial and precise temporal resolution of Magnetoencephalography (MEG) to define frequency‐specific and region‐dependent patterns of oscillatory deficits in AD patients, and to demonstrate the earliest neurophysiological abnormalities associated with AD neuropathology and their mechanistic relationships to network hyperexcitability.MethodWe studied the resting brain oscillations in individuals with AD‐spectrum (n = 88) and age‐matched controls (n = 70) using MEG. In addition, subsets of participants were evaluated with positron emission tomography (PET) with Aβ and tau specific tracers and fluid biomarker including plasma P‐tau181 and Neuro‐Filament‐Light (NFL). We used statistical mixed models to investigate the associations between neural oscillations and pathology. We used a linear Neural‐Mass‐Model (NMM) in combination with empirical MEG spectra to derive the excitatory‐to‐inhibitory activity within neuronal subpopulations in AD patients. Based on standard neurophysiological monitoring we determined clinical evidence of hyperexcitability as AD with and without epileptiform activity (AD‐EPI+; AD‐EPI‐).ResultWhile increase and decrease of oscillatory activity are distinctly associated Aβ and tau, respectively, the characteristic frequency‐specific manifestation in AD was increased spectral signature within delta‐theta (2‐7Hz) and reduced spectral signature within alpha (8‐12Hz). Earliest spectral changes were detected in Aβ+ cognitively unimpaired and the increased spectral signature associated with Aβ was replicated in identical associations with plasma p‐tau181. The presence of APO‐E4 allele strengthened these associations. NMM demonstrated greater excitatory‐inhibitory‐activity in AD‐EPI+ vs. AD‐EPI‐ while these were specifically correlated with the reduced alpha spectrum which was more profound in AD‐EPI+ as well as uniquely associated with increased tau depositions.ConclusionOur results provide critical insights about potential mechanistic links between abnormal neural oscillations and cellular correlates of impaired excitatory and inhibitory synaptic functions associated with tau and Aβ, in patients with AD.

Highly sensitive single-molecule detection of macromolecule ion beams.

The analysis of proteins in the gas phase benefits from detectors that exhibit high efficiency and precise spatial resolution. Although modern secondary electron multipliers already address numerous analytical requirements, additional methods are desired for macromolecules at energies lower than currently used in post-acceleration detection. Previous studies have proven the sensitivity of superconducting detectors to high-energy particles in time-of-flight mass spectrometry. Here, we demonstrate that superconducting nanowire detectors are exceptionally well suited for quadrupole mass spectrometry and exhibit an outstanding quantum yield at low-impact energies. At energies as low as 100 eV, the sensitivity of these detectors surpasses conventional ion detectors by three orders of magnitude, and they offer the possibility to discriminate molecules by their impact energy and charge. We demonstrate three developments with these compact and sensitive devices, the recording of 2D ion beam profiles, photochemistry experiments in the gas phase, and advanced cryogenic electronics to pave the way toward highly integrated detectors.

Pharmacokinetics of Novel Dopamine Transporter Inhibitor CE-123 and Modafinil with a Focus on Central Nervous System Distribution.

S-CE-123, a novel dopamine transporter inhibitor, has emerged as a potential candidate for cognitive enhancement. The objective of this study was to compare the tissue distribution profiles, with a specific focus on central nervous system distribution and metabolism, of S-CE-123 and R-modafinil. To address this objective, a precise liquid chromatography-high resolution mass spectrometry method was developed and partially validated. Neuropharmacokinetic parameters were assessed using the Combinatory Mapping Approach. Our findings reveal distinct differences between the two compounds. Notably, S-CE-123 demonstrates a significantly superior extent of transport across the blood-brain barrier (BBB), with an unbound brain-to-plasma concentration ratio (Kp,uu,brain) of 0.5, compared to R-modafinil's Kp,uu,brain of 0.1. A similar pattern was observed for the transport across the blood-spinal cord barrier. Concerning the drug transport across cellular membranes, we observed that S-CE-123 primarily localizes in the brain interstitial space, whereas R-modafinil distributes more evenly across both sides of the plasma membrane of the brain's parenchymal cells (Kp,uu,cell). Furthermore, our study highlights the substantial differences in hepatic metabolic stability, with S-CE-123 having a 9.3-fold faster metabolism compared to R-modafinil. In summary, the combination of improved BBB transport and higher affinity of S-CE-123 to dopamine transporters in comparison to R-modafinil makes S-CE-123 a promising candidate for further testing for the treatment of cognitive decline.

Imaging quantum oscillations and millitesla pseudomagnetic fields in graphene

The exceptional control of the electronic energy bands in atomically thin quantum materials has led to the discovery of several emergent phenomena1. However, at present there is no versatile method for mapping the local band structure in advanced two-dimensional materials devices in which the active layer is commonly embedded in the insulating layers and metallic gates. Using a scanning superconducting quantum interference device, here we image the de Haas–van Alphen quantum oscillations in a model system, the Bernal-stacked trilayer graphene with dual gates, which shows several highly tunable bands2–4. By resolving thermodynamic quantum oscillations spanning more than 100 Landau levels in low magnetic fields, we reconstruct the band structure and its evolution with the displacement field with excellent precision and nanoscale spatial resolution. Moreover, by developing Landau-level interferometry, we show shear-strain-induced pseudomagnetic fields and map their spatial dependence. In contrast to artificially induced large strain, which leads to pseudomagnetic fields of hundreds of tesla5–7, we detect naturally occurring pseudomagnetic fields as low as 1 mT corresponding to graphene twisting by 1 millidegree, two orders of magnitude lower than the typical angle disorder in twisted bilayer graphene8–11. This ability to resolve the local band structure and strain at the nanoscale level enables the characterization and use of tunable band engineering in practical van der Waals devices.

High-resolution three-dimensional dosimetry in clinically relevant volumes utilizing optically stimulated luminescence.

The continued development of new radiotherapy techniques requires dosimetry systems that satisfy increasingly rigorous requirements, such as high sensitivity, wide dose range, and high spatial resolution. An emerging requirement is the ability to read out doses in three dimensions (3D) with high precision and spatial resolution. A few dosimetry systems with 3D capabilities are available, but their application in a clinical workflow is limited for various reasons, primarily originating from their chemical nature. The search for a 3D dosimetry system with potential for clinical implementation is thusongoing. To demonstrate the capabilities of a novel optically-stimulated-luminescence (OSL)-based 3D dosimetry system capable of measuring radiation doses in clinically relevantvolumes. A laser-based readout system was used to measure dose distributions delivered by both photons and protons, utilizing the OSL from a mm YSO:Ce crystal. A homogeneous treatment plan consisting of two opposing photon fields was used to establish an inhomogeneity correction map of the crystal response and demonstrated the accuracy and precision of the system. The crystal was additionally irradiated with a photon treatment plan consisting of three overlapping mm fields delivered from different angles, and a proton treatment plan consisting of four pencil beams with energies 90 MeV ( ), 115 MeV, and 140 MeV. The system abilities were quantified by comparing the 3D-resolved measurements to Monte Carlosimulations. The dose map reproducibility of the system was found to be within 2% including both statistical and systematic errors. The measurements yielded integrated doses from a volume of mm with voxel volumes of just mm . An excellent agreement between the 3D-resolved measurements and the simulations was found for both photon- andproton-irradiation. The capabilities of the devised system for measuring clinically relevant fields of photons and proton pencil beams within a clinically relevant volume were demonstrated. The system poses as a promising candidate for clinical applications, and enables future research in the field of OSL-based tissue-equivalent 3Ddosimetry.

Novel Compact Polarized Martian Wind Imaging Interferometer

The Mars Atmospheric Wind Imaging Interferometer offers several advantages, notably its high throughput, enabling the acquisition of precise and high vertical resolution data on the temperature and wind fields in the Martian atmosphere. Considering the current absence of such an Interferometer, this paper introduces a novel Mars wind field imaging interferometer. In analyzing the photochemical model of O2 (a1Δg) 1.27 μm molecular airglow radiation in the Martian atmosphere and considering the impact of instrument signal-to-noise ratio (SNR), we have chosen an optical path difference (OPD) of 8.6 cm for the interferometer. The all-solid-state polarized wind imaging interferometer is miniaturized by incorporating two arm glasses as the compensation medium in its construction, achieving the effects of field-widening and temperature compensation. Additionally, an F-P Etalon is designed to selectively filter the desired three spectral lines of O2 dayglow, and its effect is evaluated through simulations. The accuracy of the proposed compact Mars polarized wind imaging interferometer for detecting Mars’ wind field and temperature field has been validated through rigorous theoretical derivation and comprehensive computer simulations. The interferometer boasts several advantages, including its compact and small size, static stability, minimal stray light, and absence of moving parts. It establishes the theoretical, technological, and instrumental engineering foundations for future simultaneous static measurement of Martian global atmospheric wind fields, temperature fields, and ozone concentrations from spacecraft, thereby significantly contributing to the dataset for investigating Martian atmospheric dynamics.

Reassessing the anomalous low frequencies in the Japanese koto

A previous study reported on a finite element model of an indigenous Japanese 13-stringed zither (the koto) based on a CT scan. Frequencies above 100 Hz were relatively easy to account for, while those below 100 Hz were more problematic. Low frequencies have also been reported for the piano, another zither of similar length. The model has now been updated in a range of critical areas, including damping. The updates have resulted in a more precise resolution of the model. It allows us to reassess the anomalous low frequencies to determine if they have other rational explanations. This work continues to expand knowledge of the koto’s complex resonances and has broader applications for understanding the characteristic sounds of other wooden indigenous musical instruments.

Combined Radiotherapy Using a Method for Biologically Informed IMRT Planning

Radiotherapy (RT) regimens routinely employ multiple courses that vary by delivery modality and/or fractionation schedule as part of initial or follow up treatment. The combination of RTs ideally should consider the biological effect of the total composite dose distribution. Biological effect can be quantified by biological effective dose (BED) which is a function of the RT modality, dose fractionation, and tissue radiosensitivity. This work describes a method of optimizing a follow up intensity modulated RT (IMRT) plan that considers BED from prior RT. Required inputs for this method are the desired composite BED objectives (BEDref) for target volume(s) and organs at risk (OARs). First, the physical dose in each CT voxel from prior RT (Dprior) is converted to BED (BEDprior). The difference between BEDref and BEDprior gives the optimal BED distribution for the follow up IMRT plan (BEDIMRT). Next, the optimal physical dose (DIMRT) corresponding to BEDIMRT is calculated for each voxel as a function of the number of IMRT fractions (nIMRT). Commercial IMRT treatment planning systems require optimization objectives to be specified for contoured structures, not individual voxels, so a workaround is proposed: 1) for each structure, BEDref is converted to physical dose (Dopti) as a function of nIMRT, and 2) for each voxel, the difference of Dopti and DIMRT is calculated. The result is a "base dose distribution" (Dbase) that is used with standard inverse optimization tools to optimize an IMRT plan so that the sum of Dbase and the IMRT dose approaches Dopti for each voxel. This method was implemented using a custom software workflow to calculate Dbase as a function of Dprior and input BEDref values. Validation was performed by comparing the workflow output with hand calculated Dbase values at randomly selected points within clinically significant regions. Satisfactory agreement was considered to be within 5%. Feasibility of the method was demonstrated using an RT trial protocol for glioblastoma multiforme that combines permanent low dose rate brachytherapy (BT) using collagen tiles embedded with 131Cs seeds and follow-up IMRT (20 fractions). The Dbase distribution was obtained from the software as a function of the BT dose and BEDref for one patient model. Comparison of Dbase values from the software and hand calculated values resulted in agreement within 5% for 22/25 points and within 10% for 3/25 points. Discrepancies >5% (max 9.4%) were attributed to data precision resolution effects inherent in the DICOM standard. The resulting IMRT plan was deemed clinically acceptable and the composite dose distribution achieved dosimetric objectives for all target and OARs. A method for optimizing a follow up IMRT plan that considers dose from prior RT to produce a desired composite BED distribution was derived and proof of concept was successfully demonstrated. Further validation of this method with additional patients and multimodality RT protocols will be performed.

Investigating the ERA5-Based PWV Products and Identifying the Monsoon Active and Break Spells with Dense GNSS Sites in Guangxi, China

Precipitable water vapor (PWV) with high precision and high temporal resolution estimated by Global Navigation Satellite System (GNSS) is widely used in atmospheric research and weather forecasting. However, most previous works are not consensual concerning the characteristics of the PWV at different time scales and the identification of active and break spells during summ er monsoon climate in Guangxi, China. Taking radiosonde (RS) observations as reference, a strong correlation (R > 0.97) exists between GNSS PWV and RS PWV with a mean root mean square error (RMSE) of 2.68 mm. The annual, seasonal, monthly, and diurnal PWV variations of three years (2017, 2018 and 2020) over Guangxi in were comprehensively investigated using 104 GNSS stations and the fifth-generation European Centre for Medium-Range Weather Forecasts (ECMWF) Atmospheric Reanalysis (ERA5). The mean annual bias and RMSE between GNSS PWV and ERA5 PWV are −1.04 mm and 2.63 mm, respectively. The monthly bias and RMSE range are −0.77 to 3.87 mm, 1.32 to 4.45 mm, and the daily range is −1.41 to 1.07 mm and 1.11 to 5.02 mm, respectively. Additionally, the adopted average standardized rainfall anomaly criteria also identified 7/7/3 active spells and 5/3/7 break spells during the summer monsoon (June–September) from 2017 to 2020, respectively. During the three-year period, the daily amplitude ranges for active spells varied from 1.41 to 2.49 mm, 0.69 to 5.4 mm, and 0.88 to 1.41 mm, while the ranges for break spells were 2.45 to 6.76 mm, 1.66 to 8.17 mm, and 1.48 to 2.99 mm, respectively. The results show a superior performance of GNSS PWV compared to ERA5 PWV in Guangxi, and the maximum, minimum and occurrence time of PWV anomaly vary slightly with the season and the topography of stations. Despite temperature primarily exhibiting a negative correlation with rainfall, acting as a dampener, a positive correlation remains evident between PWV and rainfall. Therefore, densely distributed GNSS stations exhibit excellent capabilities in quantifying atmospheric water vapor and facilitating real-time monitoring of small and medium-scale weather phenomena.

Tracing textual silences and ideological tensions in adopted inclusive education legislation in China

A substantial body of literature exists on inclusive education practices in large countries like China, ranging from the introduction of laws and regulations to cross-country comparisons of the implementation of policies. Yet, little is known about the potential role that legal documents play in shaping ideological assumptions and actions among stakeholders, including parents. The authors used critical discourse analysis (van Dijk, 1993) as an analytic guide for exploring explicated and implied meanings within both legal texts associated with China's “learning in the regular classroom” (LRC) model as well as reported data from parents of children diagnosed with autism spectrum disorder (ASD) about their experiences advocating for inclusive education in China through interpretative phenomenological analysis (Eatough & Smith, 2017). By examining the connections between discursive practice and social structures embedded in an institutional use of legal discourse and delving into the unique experiences of parents as insiders, the authors discovered not only that textual silence exists in LRC model legal texts regarding obligation, text precision, and dispute resolution but that it also influenced parents of children with ASD as they navigated and advocated for their children under the LRC model. This study is an extension of an earlier investigation of parental perspectives (Zhang, Qian & Singer, 2022). By revealing the typically obscured assumptions and implications of educational policies, our study demonstrates the potential significance and benefits of conducting similar research in different national contexts.

Towards Nearly Zero-Energy Buildings: Smart Energy Management of Vehicle-to-Building (V2B) Strategy and Renewable Energy Sources

As countries worldwide strive for net-zero emissions, electric vehicles (EVs) have gained significant momentum. EVs, particularly through Vehicle-to-Everything (V2X) technology, including Vehicle-to-Building (V2B), are recognized as key enablers for achieving nearly-zero energy buildings (NZEB). This research presents an advanced Smart Energy Management System (SEMS) with a novel V2B strategy, offering precise one-hour resolution to reduce grid reliance and optimize peak load management. The SEMS coordinates building energy and EV charging while harnessing the potential of renewable energy resources and battery storage systems. By carefully scheduling EV charging and discharging activities, it maximizes the efficient use of available energy resources. Importantly, the algorithm integrates real-world EV parking patterns, accounting for factors such as fleet composition, parking durations, and charging priorities. Economic evaluation encompasses not only direct EV battery degradation costs but also external expenses associated with environmental impacts. Simulation outcomes underscore a remarkable reduction in building grid power demand (65%) and a substantial decrease in carbon emissions (64%). The PV+V2B Scenario emerges as the preferred choice, striking an optimal balance between initial investment costs, energy savings, and carbon footprint reductions. This study holds great academic and practical significance, contributing to the advancement of smart, sustainable, and economically viable net-zero cities.

Three-dimensional oxygen concentration monitoring in hydrogels using low-cost phosphorescence lifetime imaging for tissue engineering.

Oxygen concentration measurement in 3D hydrogels is vital in 3D cell culture and tissue engineering. However, standard 3D imaging systems capable of measuring oxygen concentration with adequate precision are based on advanced microscopy platforms, which are not accessible in many laboratories due to the system's complexity and the high price. In this work, we present a fast and low-cost phosphorescence lifetime imaging design for measuring the lifetime of oxygen-quenched phosphorescence emission with 0.25 µs temporal precision and sub-millimeter spatial resolution in 3D. By combining light-sheet illumination and the frequency-domain lifetime measurement using a commercial rolling-shutter CMOS camera in the structure of a conventional optical microscope, this design is highly customizable to accommodate application-specific research needs while also being low-cost as compared to advanced instruments. As a demonstration, we made a fluidic device with a gas-permeable film to create an artificial oxygen gradient in the hydrogel sample. Dye-embedded beads were distributed in the hydrogel to conduct continuous emission lifetime monitoring when nitrogen was pumped through the fluidic channel and changed oxygen distribution in the sample. The dynamics of the changes in lifetime co-related with their location in the gel of size 0.5 mm×1.5 mm×700 µm demonstrate the ability of this design to measure the oxygen concentration stably and precisely in 3D samples.