Poly Herbal Research Articles

Cow Urine Mediated Poly Herbal Formulation Preparation, Characterization, and Assessment of Antiglycation and Anti-Diabetic Activity

present study has the objective of preparing, characterizing, and evaluating the antiglycation and antidiabetic activity of polyherbal formulations mediated by cow urine. The formulations were prepared using various herbs, and their phytochemical constituents were characterized using standard methods[1]. The formulations' ability to prevent the formation of advanced glycation end products (AGEs) was assessed by evaluating their antiglycation activity.[2,3], The antidiabetic activity was assessed by measuring their ability to inhibit α-amylase and α-glucosidase enzymes. The findings revealed that the polyherbal formulations formulated with cow urine exhibited notable antiglycation and antidiabetic properties compared to the control groups. The study concludes that polyherbal formulations mediated by cow urine hold potential therapeutic applications in the management and treatment of diabetes. [1].

Formulation and Evaluation of Poly Herbal Body Scrub

The research aimed to produce an Polyherbal body scrub. These days, the demand for skin care products and treatments has increased to a larger extend.Having a proper appearance and a beauty standard has gotten a lot of importance. A typical skin care routine consists of a cleanser, a serum, a moisturizer and a sunscreen.Among these, it has been seen that the serums are the new go to when it comes to building an excellent skin routine. Serums come in various types of formulation be it for oily, dry or anything in between type of skin. Facial wrinkles and skin aging are undesirable outcome of photo damage and ultraviolet (UV) rays. Currently no effective strategies are available to delay skin aging process. Aloe Vera, glycerin and honeybee venom face serum is a highly concentrated cosmetic product. When using aloe Vera we get not only a quick cosmetic effects but also psychological satisfactions, serum has a property of rapid absorption and ability to penetrate into deeper layer of the skin. In the present work we have formulated the poly herbal body scrub by using Orange peel powder, SCI powder, SLS powder, Honey, Glycerine, Bees wax, Aloe vera. These formulations are safe to use for skin and found to be satisfied with all required characterizations. The scrub was evaluated by using the parameters like Appearance, Viscosity, pH, Spreadibility, Washability, Grittiness, Foamability, Moisture content, Consistancytest and Irritation.

In Vitro Immunomodulatory Activity of Sagalanoi Chooranam (Poly Herbal Formulation) in RAW Macrophage Cell Line

Within the realm of Siddha medicine, an ancient and venerable traditional healing system hailing from India, a comprehensive methodology is deployed to combat liver disorders. This approach is characterised by the utilisation of herbal remedies, accentuating the reinstatement of equilibrium in the body's vital life force, known as "Vatham," "Pitham," and "Kapham." The utilisation of cell line studies is crucial in assessing the immunomodulatory properties of herbal medicine, offering invaluable insights into their capacity to influence the immune system and advancing our comprehension of their therapeutic benefits. The primary objective of this investigation was to elucidate the potential immunomodulatory properties of sagalanoi chooranam, a polyherbal Siddha formulation, through the execution of anti-proliferative assays conducted. On the RAW 264.7 macrophage cell line. Various test solutions were precisely prepared, encompassing a spectrum of concentrations (50, 100 and 200 μg/ml). The RAW 264.7 cells were diligently cultured and then carefully subjected to stimulation with lipopolysaccharide (LPS) to initiate cellular activation. Following this activation, the test formulation was introduced at varying concentrations, after which the cells underwent a 24-hour incubation period. The resultant nitrite levels, serving as indicators of immunomodulatory response, were evaluated within the cell lysate. The outcomes unveiled a notable decline in nitrite levels, correlating with the dosage of the test formulation during the incubation with RAW 264.7 cells. The outcomes of the study elucidate a conspicuous decline in nitrite levels in direct correlation with escalating concentrations of the test formulation, contrasting starkly with the control group exclusively subjected to LPS. Furthermore, the investigation meticulously probed the ramifications of the test formulation on cellular viability. The vitality of RAW 264.7 cells exhibited a discernible downward trajectory as the concentration of the test formulation ascended. Noteworthy is the fact that, at the concentration of 200 μg/ml, cellular viability registered at a mere 55.53 ± 3.567%. Collectively, these findings lend credence to the hypothesis that the test formulation possesses a dose-dependent capacity to attenuate both nitrite levels and cellular viability within the RAW 264.7 cell milieu, underscoring its auspicious immunomodulatory attributes. This research expands our insights into the potential immunological ramifications of the test formulation and underscores its plausible utility within the realm of immunomodulation.

Antibacterial and Mosquito Repellent Assay of Poly Herbal Oil

Antibacterial and Mosquito Repellent Assay of Poly Herbal Oil

A Dual Therapeutic Approach to Diabetes Mellitus via Bioactive Phytochemicals Found in a Poly Herbal Extract by Restoration of Favorable Gut Flora and Related Short-Chain Fatty Acids.

Diabetes mellitus (DM), a metabolic and endocrine condition, poses a serious threat to human health and longevity. The emerging role of gut microbiome associated with bioactive compounds has recently created a new hope for DM treatment. UHPLC-HRMS methods were used to identify these compounds in a poly herbal ethanolic extract (PHE). The effects of PHE on body weight (BW), fasting blood glucose (FBG) level, gut microbiota, fecal short-chain fatty acids (SCFAs) production, and the correlation between DM-related indices and gut microbes, in rats were investigated. Chebulic acid (0.368%), gallic acid (0.469%), andrographolide (1.304%), berberine (6.442%), and numerous polysaccharides were the most representative constituents in PHE. A more significant BW gain and a reduction in FBG level towards normal of PHE 600mg/kg treated rats group were resulted at the end of 28th days of the study. Moreover, the composition of the gut microbiota corroborated the study's hypothesis, as evidenced by an increased ratio of Bacteroidetes to Firmicutes and some beneficial microbial species, including Prevotella copri and Lactobacillus hamster. The relative abundance of Bifidobacterium pseudolongum, Ruminococcus bromii, and Blautia producta was found to decline in PHE treatment groups as compared to diabetic group. The abundance of beneficial bacteria in PHE 600mg/kg treatment group was concurrently associated with increased SCFAs concentrations of acetate and propionate (7.26nmol/g and 4.13nmol/g). The findings of this study suggest a promising approach to prevent DM by demonstrating that these naturally occurring compounds decreased FBG levels by increasing SCFAs content and SCFAs producing gut microbiota.

Design and Evaluation of Poly Herbal Mosquito Repellent Incense Sticks

The current study's goal was to create multi-herb mosquito-repellent incense sticks. Two formulations were made F1 and F2. The F1 formulation contains less quantity of poly herbal ingredients than the F2 formulation. The developed formulation's effectiveness as a mosquito repellent was assessed. The Incense mosquito sticks were prepared by hand rolling method in research laboratory. The herbal ingredients used are the Holy basil, Neem, Rosemary, Camphor, Cinnamon bark, lemongrass oil. The Acacia used as a binder to roll the sticks and lemon grass oil used as flavoring agent. The prepared formulation F1 and F2 was evaluated its efficacy. The F2 formulation containing increased quantity of poly herbs so it shows better repellent activity than F1 formulation. So the uses of natural herbs in the formulation of mosquito repellent sticks are free from carcinogenic chemicals, cheaper, and easy to formulate.

Standardization Quality Control and Development of Poly Herbal Formulation for the Management of Type-2 Diabetes Mellitus

In the Ayurvedic system of medicine, Herbal Drugs For Diabetes Mellitus as mentioned in ancient Indian books like Charak Samhita, Mahdhav Nidan and Astang Sanghra, there are about 600 plants, which are stated to have antidiabetic property Polyherbal antidiabetic formulation consists of six herbs viz., Nigella sativa (seed), Moringa oleifera (seed), Linum usitatissimum (seed), Trogonella foenum (seed), Cinnamum zeylanicum (bark) and Macrotylom auniflorum (seed). Crospovidone, Microcrystalline cellulose, Colloidal Silicon dioxide, PVP Magnesium stearate, (Polyvinyl pyrrolidone). Polyherbal formulation of the extracts of all selected plants was subjected to freeze drying process. The extracts were dried for a period of time according to their rate of drying .Diluents like, Microcrystalline cellulose, Magnesium stearate, Lactose, starch were dried. All active ingredients were weighed according to the formula, mixed with MCC followed by diluents and Glidant like aerosil and magnesium stearate as lubricant as specified in formula were mixed well. The mixture was blended thoroughly for 30minutes. Then the powder was transferred to the polythene bags and labeled for further studies.

Clinical Effect of Poly Herbal Unani Formulation on Dyslipidemia- A Randomized Trial

Background: In adults aged 30-70 with primary and moderate hyperlipidemia, the present study took place to investigate the therapeutic benefits of a polyherbal unani preparation called Garlitab. Methods: It was a prospective open label, herbal coded test drug-controlled, randomized trial. Out of total screened patients we were enrolled 212 hyperlipidemic patients of 30–70 years in the study fulfilling the inclusion criteria, we were recruited them from OPD of a hospital in Munshiganj and different Unani clinics of Dhaka, Bangladesh after obtaining written informed consent from the patients. Selected individuals were allocated into two groups at random. Group1 Received 500 mg Garlitab tablets twice daily and Group 2 received tablet atorvastatin calcium 10 mg 2 times daily. Height, weight, and blood pressure were recorded along with blood samples. The random distributions were carried out by a research assistant utilizing a random numbers table. Blood samples were taken at the beginning of the trial, 1.5 months later, and 3 months following the intervention. Results: Results for the test medication revealed a substantial drop in cholesterol levels between baseline and the data collected after three months and in case of male it was from 241.72±38.11 to 218.24±34.06 mg/dL for total cholesterol, from 198.27±30.57 to 173.54±29.34 mg/dL for LDL and from 280.78±85.81 to 207.07±51.40 mg/dL for triglyceride. HDL increases from 33.05±3.21 to 34.69±3.13 mg/dL in male patients. The control drug atorvastatin calcium also showed a significant decrease in lipids between baseline and after 3 months data and in case of male it was from 241.92±31.54 to 174.90±22.87 mg/dL for total cholesterol, from 196.20±30.91 to 130.30±24.29 mg/dL for LDL and from 279.48±115.35 to 141.27±59.55 mg/dL for triglyceride. It increases HDL from 32.00±2.25 to 34.03±2.19 mg/dL in male patients. Between the baseline and the 3-month data, the test medicine for females significantly reduced total cholesterol, LDL, and triglycerides and it was from 244.64±52.18 to 220.12±45.07 mg/dL, from 200.32±30.57 to 173.54±29.34 mg/dL and from 272.32±99.69 to 195.25±60.68 mg/dL respectively. HDL increases from 33.77±3.36 to 35.03±3.23 mg/dL. Between the baseline and the 3-month data, the control medication for females significantly reduced total cholesterol, LDL, and triglycerides and it was from 247.74±37.95 to 175.26±29.54 mg/dL, from 197.65±27.89 to 130.91±22.04 mg/dL and from 271.57±94.52 to 142.00±50.88 mg/dL respectively. It increases HDL from 32.22±2.32 to 33.46±2.94 mg/dL. Conclusions: According to the results of the study, the polyherbal formulation Garlitab can lower cholesterol levels. It may be a useful medication for treating primary hyperlipidemia.

Synthesis of Silver Nano-particle with Poly-herbal Plant Extracts for Anti-fungal and Anti-microbial Activity on Candida Albicans and E. Coli

In this study, we have developed a method to synthesize silver nanoparticles by mixing the silver solution with the leaf extract of Pisum sativum without using any surfactant or external energy. In this method, physiologically steady, bio-compatible Ag nanoparticles were set up. These functionalized POLY HERBAL Ag-NPs could be used for targeted drug delivery with enhanced therapeutic efficacy and minimal side effects. Extracellular synthesis of metal nanoparticles using extracts of plants like Pisum sativum (Pea), Zingiber officinale (Ginger) Curcuma longa (turmeric) has been successfully carried out. In this article, Ag-NPs formation using Pisum sativum (Pea), Zingiber officinale (Ginger) and Curcuma longa (turmeric) has been thoroughly discussed. It is well recognized that on treating the metallic salt solution with some plant extracts, a rapid reduction take place leading to the origination of highly stable metal nanoparticles. With this method quick synthesis of nanoparticles was noticed to occur; i.e., reaction time was 1–2 h as contrasted to 2–4 days required by microorganisms. These nanoparticles were examined by various characterization techniques to disclose their morphology, chemical composition, and antimicrobial activity. TEM image of these NPs shows the evolution of spherical, non-uniform, poly-dispersed nanoparticles. A comprehensive study of the anti-microbial activity of nanoparticles was performed.

Formulation and Evaluation of Poly Herbal Mouth Wash against Different Mouth Disorder

Abstract: Our oral cavity is a sweet able place to grow different types of bacterial species either harmless or harmful for human. Many of us are suffering different types of mouth disease and feeling insecure or hesitate to speak with another due to the unpleasant odor of mouth. To maintain a good oral hygiene mouthwash plays a important role in our daily lifestyle. From ancient age medicinal plants are considered as a store room of different types of biological activity in Ayurveda, Unani and Siddha, and have important role to cleanse tooth and prevent different human pathogens are responsible for unpleasant odor, inflammation of teeth root, dental plaque. Herbal mouthwash contains different medicinal plant part extract have various pharmacological activities and shows significant advantages which helps to cure different types of oral disorder and helps to maintain oral hygiene. Herbal mouthwash is suitable for any age group due to less or no other side effect instead of chemical mouthwash. Aqueous extract of Neem, Turmeric, Clove, Fennel, Guava leaves, Pudina, Ginger, Tulsishows effective antimicrobial, antifungal activity, anti-inflammatory and anti-plaque properties. In this research work herbal mouth wash was evaluated depends on various parameter like color, pH, Visual appearance, Phase separation, Homogeneity and antibacterial properties.

Formulation and Evaluation of Poly Herbal Chewable Tablets from Ayurvedic Ingredients having Cough Relieving Activity

Formulation and Evaluation of Poly Herbal Chewable Tablets from Ayurvedic Ingredients having Cough Relieving Activity

Screening of Antimicrobial Activity of Poly Herbal Extracts against Bacterial Pathogens Causing Gastroenteritis in Tharaka Nithi County, Kenya

Herbals have been used for decades to treat gastrointestinal infections across the world, including in Tharaka-Nithi County, Kenya. Crude extracts from herbs have long been prescribed by traditional healers for treatment of typhoid, cholera, and shigellosis. However, the effectiveness of extracts obtained using different extraction solvents such as methanol, acetone, and hexane have not fully been evaluated. This study aimed at evaluating the effect of solvents (Ethanol, hexane, and methanol) on the yield of crude extract from plants (Erythrinna abyssinica, Aspilia pluriseta, Vangueria infausta, Ficus sycomorus, and Carissa edulis), assessing the effect of the solvents on extracted active metabolites, and determining the effect of these solvents on extract’s bioactivity against Vibrio cholerae, Shigella flexneri, and Salmonella typhimuriumi and, Escherichia coli that served as a model organism for screening plant extracts against Gram-negative bacteria. Crude extracts were obtained by soaking dried ground plant parts in individual solvents, which were then concentrated by a rotary evaporator. The phytochemical screening to detect plant metabolites was done qualitatively. Bioassays to analyze the efficacy of the plant crude extracts against the microbes were carried out in 4×3×3×5 factorial experiment laid out in a completely randomized design. The determination of bioactivity of herbal extracts was carried out using minimal inhibition concentration and minimum bactericidal concentration methods. Data obtained on the bioactivity assay (Count of the bacteria colony forming units) was analyzed using the Kruskal-Wallis test at α = 0.05, and medians were compared by the Wilcoxon rank sum test in Scientific Analysis System version 9.4. Methanol solvent produced higher quantities of crude extracts for all the herbal samples used with, Vangueria infausta producing the highest extract (5.06g). Most phytochemicals were present in Methanolic extracts compared to hexane and acetone extracts. There was a significant (p < 0.05) difference in the bioactivity of different herbal plants against bacterial pathogens at different concentrations of crude extract. The efficacy of plant extract increased progressively from 100ppm to 1000ppm concentration. Methanol is recommended for use in the extraction of medicinal plant extracts as it leads to improved potency as compared to hexane and acetone.

In-vivo studies to determine Hair Growth Potential of Poly Herbal Medicated Hair Oil in Female Swiss Albino Mice

Hair loss is one of biggest problem of today’s human life throughout World. Alopecia, Baldness and hair loss are commonly found in the men as well as in female. Commonly available medicated hair oils in market for the treatment of hair disease are made up with mineral oil, which is proved to be carcinogenic in nature if applied for longer period of time. Hair plays a vital role in the personality of human and for their cure we use lots of cosmetic products. The fading (pigmentation problem), dandruff, alopecia (loss of hair) is the major problem associated with hairs. An Ayurveda is Indian traditional system of Medicine having major treatment across globe. The aim of study is to develop a poly herbal hair oil (PHO) formulation using Azadirachta indica, Rosa sinensis, Muraya koenigii, Lagenaria siceraria, Bacopa monieri, Lawsonia inermis, Eclipta alba, Embelica officinalis, coconut oil and Til oil, for better growth of hair and diminution in loss of hair (alopecia). The oil was prepared according to Ayurvedic Formulary of India and was standardized according to Protocol for Testing Ayurvedic, Siddha and Unani Medicines, Government of India. The physico-chemical evaluation was carried out for medicated hair oil. The pharmacological evaluations were performed through Pre-clinical Animal studies for qualitative, quantitative and mean- hair length studies.The statistical results of the study have demonstrated that 5% PHO possessed potent action on hair growth in the Female Swiss albino Mice animal’s model investigates as well as these provide a rationale in favor of its uses inside traditionally drug in favor of utilization in Baldness and other hair growth disorders.

GC-MS and Antioxidative Effects of Poly Herbal Formulation of Persea americana Seed Against Ethanol-Induced Gastric Ulcer in Rats

GC-MS and Antioxidative Effects of Poly Herbal Formulation of Persea americana Seed Against Ethanol-Induced Gastric Ulcer in Rats

In-Vitro Anti-inflammatory Activity of Traditionally reported Poly Herbal Combination

Inflammatory diseases are still one of the most important heath-problems in the world. Most of anti-inflammatory drugs such as non–steroidal anti-inflammatory drugs (NSAIDS) have number of adverse effects hence there are a need to develop safe and new anti-inflammatory formulation with minimum side effects. Present study focused on investigating and comparing anti-inflammatory potential of hydroalcoholic extracts of Tinospora cordifolia, Boerhavia diffusa, Tribulus terrestris and their combinations. Inhibition of heat induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The combination ratio of hydroalcoholic extracts of Tinospora cordifolia, Boerhavia diffusa, Tribulus terrestris (2:5:3) showed the most significant membrane stabilizing action on HRBC membrane as compare tohydroalcoholic extracts of individual drugs and theirother combinations. The present study revealed that the proper combination of Tinospora cordifolia, Boerhavia diffusa, Tribulus terrestris gives more effective anti-inflammatory activity than any of the individual drug.

Effect of Polyherbal Formulation (PHF) and its comparative study with other treatment in different types of dermatitis (Eczema) in experimental animals

Atopic dermatitis (AD) and allergic contact dermatitis are skin disorders that are a type of eczema. AD is a childhood disorder & allergic contact dermatitis. When the skin touches irritating bacteria, the skin inflammation causes itching and redness. The Poly Herbal Formulation uses this content: chaulmoogra oil 10%, neem oil 8%, and Karanja oil 30%, then made into an ointment. They are using this ointment topically for mice's skin after 30 days of redness, itching, dryness, and inflammation necessary for the basic treatment. This paper provides a different study of eczema AD & contact allergic dermatitis by using a polyherbal formulation.

In-vitro Antiplasmodial Efficacy of a Poly Herbal Formulation against Plasmodium falciparum (3D7 Strain)

Malaria is one of the world's leading killer infectious diseases accounting for about 241 million cases of infection and 627000 deaths all around the globe in the year of 2021. In the present study, a polyherbal formulation used by traditional drug practitioners to cure malarial infection in some rural areas of Barpeta district of Assam, India was tested in vitro for its antiplasmodial and antioxidant properties. Two plant species namely Aristolochia indica L. and Piper nigrum L. were used for the herbal formulation. Water extract along with other organic solvent extracts viz. Chloroform, 2-propanol, methanol and n-hexane was tested in the experiment. Methanol and 2-propanol extracts in high concentration (50µg/ml) showed promising antiplasmodial effect in 3D7 strain of Plasmodium falciparum. Water extract showed promising antioxidant properties along with methanolic extract. This preliminary study conclusively established the antiplasmodial and antioxidant properties of the traditionally used polyherbal formulation. Further studies are required for the exploration of bioactive compounds from this formulation. Scientific validation of such traditional practices is important not only because there is an urgent need for alternative medication against diseases like malaria, but it is also important from the conservation point of view. These traditional herbal remedies lack proper documentation in scientific literature and the need of the hour is to explore their potential and proper documentation.

Canine Osteoarthritic Pain and Joint Inflammation: The Role of Poly Herbal Formulations

Canine Osteoarthritic Pain and Joint Inflammation: The Role of Poly Herbal Formulations

"Development and Standardization of a Poly Herbal Formulation"

"Development and Standardization of a Poly Herbal Formulation"

Preparation a Novel Poly Herbal Antibacterial Formulation, Characterization and Evaluation of Mouth Wash Protecting and Tackling Oral Cavity against Oral Pathogens

The oral cavity is the home of various bacterial species. All through some of the oral bacteria are harmless and there are certain species that are harmful which may causes oral plaque, bad breath and mouth disease. Medicinal plant or herbs are considered to rich source of ingredients which can be used in drug development. They can prevent and cure disease because of their antimicrobial and antibacterial property against microorganisms. The importance of mouth and teeth cleanliness has been recognized from the earliest days of civilization. Patients and oral health practitioners are faced with a multitude of mouthwash products containing many different active and inactive ingredients. Natural mouthwashes may offer significant advantages over the chemical ones. If such mouthwashes can be formulated which can be easily prepared and used safely by people at home using natural products, it may leads to improvement in the general dental health of the population. In this study the various natural ingredients and materials are used like Leaves of Azadiracta indica (neem), Mentha Piperita (peppermint), Ocimum sanctum (Tulsi) bud of Eugenia caryophyllus (clove), and bark of Cinnamomum verum (cinnamon), Glycyrrhiza glabra (Licorice) Although many popular herbal products have helped to control dental plaque and gingivitis, they have been used for a short time and only as an adjunct to other oral hygiene measures such as brushing and flossing. By this study it was concluded that the developed herbal mouthwash possess significant, therapeutically efficacious, suitable vehicle for drug delivery in low cost but definitely with high potential. Hence there is need for increased usage of herbal preparation to avoid the adverse effects