Synthesis of Silver Nano-particle with Poly-herbal Plant Extracts for Anti-fungal and Anti-microbial Activity on Candida Albicans and E. Coli

Abstract

In this study, we have developed a method to synthesize silver nanoparticles by mixing the silver solution with the leaf extract of Pisum sativum without using any surfactant or external energy. In this method, physiologically steady, bio-compatible Ag nanoparticles were set up. These functionalized POLY HERBAL Ag-NPs could be used for targeted drug delivery with enhanced therapeutic efficacy and minimal side effects. Extracellular synthesis of metal nanoparticles using extracts of plants like Pisum sativum (Pea), Zingiber officinale (Ginger) Curcuma longa (turmeric) has been successfully carried out. In this article, Ag-NPs formation using Pisum sativum (Pea), Zingiber officinale (Ginger) and Curcuma longa (turmeric) has been thoroughly discussed. It is well recognized that on treating the metallic salt solution with some plant extracts, a rapid reduction take place leading to the origination of highly stable metal nanoparticles. With this method quick synthesis of nanoparticles was noticed to occur; i.e., reaction time was 1–2 h as contrasted to 2–4 days required by microorganisms. These nanoparticles were examined by various characterization techniques to disclose their morphology, chemical composition, and antimicrobial activity. TEM image of these NPs shows the evolution of spherical, non-uniform, poly-dispersed nanoparticles. A comprehensive study of the anti-microbial activity of nanoparticles was performed.