Plant-mediated Synthesis Research Articles

Bioconjugate synthesis, phytochemical analysis, and optical activity of NiFe2O4 nanoparticles for the removal of ciprofloxacin and Congo red from water

In this paper, Jr.NiFe2O4 nanoparticles (NPs) were synthesized first time using the leaves extract of Juglans regia via a straightforward process. The physio and phytochemical analysis of plant confirm the presence of macromolecules which function as bio-reductant and stabilize the nanoparticles. The Jr.NiFe2O4 NPs were characterized by UV–visible, FTIR spectroscopy, PXRD pattern, SEM and TGA/DTA analysis. The nanoparticles proved to be optically active having a value of indirect bandgap of energy in the range of 1.53 eV. The Jr.NiFe2O4 NPs have the ability in scavenging 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) free radicals and showed 58.01% ± 1.2% scavenging activity at 100 µg/mL concentrations. The photocatalytic degradation study of ciprofloxacin (CIP) and Congo red (CR) reveals that the highest degradation rate was acquired for CIP using pH = 3, at 254 nm, while 85% of removal rate was analysed for CR. The kinetic studies in case of CR removal followed pseudo-first-order model with thermodynamic parameters (∆G° = − 5.87 kJ mol−1 K, ΔH° = 1393.50 kJ mol−1 and ΔS° = 22.537 kJ mol−1 K) with error analysis. Overall, these data recommend an innovative inspiring application of a plant-mediated synthesis of Jr.NiFe2O4 NPs.

Dates (Phoenix Dactylifera L.) extracts derived nanoparticles and its application

Plant-mediated green synthesis of metallic nanoparticles (NPs) has become the most deserving alternative to chemical synthesis as this process is economical and energy-efficient, and environmentally benign. For the last twenty to thirty years, different plant sources are being utilized for the fabrication of green NPs, and few of them have used the extract of Phoenix Dactylifera L. as reducing, capping, or stabilizing agents. This review provides a detailed outline of the extraction method from various parts of dates and their synthesis with different metal salts using these extracts. The applied techniques of characterization and application of these nanoparticles have also been thoroughly discussed. The phytochemicals present in the extract were responsible for reducing the metals. Except for a few, all the investigations reported the spherical NPs but have variations in their size. These NPs have high prospects in applications such as antimicrobial, anticancer, antioxidant, and catalytic activities. This work may lead the path for additional advancement in this field, and researchers may take up the future work for the large scale production of NPs and their application using date extracts.

Chemical and Plant Mediated Synthesis of La2O3 Nanoparticles and Comparison of their Structural, Antibacterial, Photocatalytic and Optical Properties

The La2O3 nanoparticles have been synthesized successfully with a chemical and biosynthesized method. The optical bandgap energy and of chemically synthesized or biosynthesized (M. oppositifolia and T. portlacaustrum leaf extract) La2O3 nanoparticles was calculated from UV-visible absorption between 5.10, 4.26 and 4.46 eV. The good polycrystalline cubic nature of synthesized La2O3 nanoparticles was evident from the bright circular SAED pattern, consistent with the XRD outcome. It is clear that the non-polar extracts could function as stabilizers for La2O3 nanoparticles through attachment to the counterions. The La2O3 nanoparticles have been used as efficient photocatalyst to degrade acid black 1 dye under sunlight irradiation. Besides, this biocatalyst showed excellent ability to degrade biosynthesized La2O3 nanoparticles (T. portlacaustrum) under visible light irradiation 87%. Synthesis of La2O3 nanoparticles by green chemistry process presented good antibacterial activity against Gram-negative and Gram-positive bacteria.

Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica Papaya Leaf Extract for Antibacterial Applications

Plant Mediated Synthesis of ZnO and Mn Doped ZnO Nanoparticles Using Carica Papaya Leaf Extract for Antibacterial Applications

Plant-Mediated Synthesis of Iron Oxide Nanoparticles and Evaluation of the Antimicrobial Activity: A Review

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BIOGENIC SYNTHESIS, CHARACTERIZATION OF SILVER NANOPARTICLES FROM CARISSA CARANDAS LEAF EXTRACT AND ITS PHARMACOLOGICAL ACTIVITIES

A Plant-mediated synthesis of nanoparticles is considered as a representative approach to environment benignity in material synthesis to avoid chemical toxicity. In this present study, we report an eco-friendly technique for the preparation of silver nanoparticles (AgNPs) from aqueous leaves extract of Carissa carandas Linn, and the evaluation of their pharmacological activities. The formation of AgNPs is confirmed by UV-Visible spectrum which peak highest at 436nm that corresponds to surface plasmon resonance of AgNPs. The shape and size of synthesized AgNPs were determined by HR-TEM and FEG-SEM which indicated well-dispersed nanoparticles with size ranging from 20nm-40nm. The functional groups responsible for reduction of silver ions are demonstrated by FTIR. XRD reported the crystalline nature of AgNPs. DPPH assay shows AgNPs has better antioxidant potential than its aqueous leaves extract. Antibacterial activity of AgNPs using resazurin dye against gram-positive and gram-negative bacteria with minimum inhibition concentrations (MICs) was found to be in the range from 31.5 μg/mL to 125 μg/mL. This biogenic synthesis provides an environment friendly, clean, cost-effective, and easy method for synthesis of AgNPs with enhanced pharmacological activities.

Phytobiological-facilitated Production of Silver Nanoparticles From Selected Non-cultivated Vegetables in Nigeria and Their Biological Potential.

Plant-mediated synthesis [silver (Ag) to form Ag nanoparticles (AgNPs)] is becoming progressively well accepted in many scientific and pharmaceutical fields. The aim of this study was to synthesize AgNPs using air-dried leaves of four neglected vegetables, i.e. Ceratotheca sesamoides, Ceiba pentandra, Crassocephalum crepidioides, and Launaea taraxacifolia. Ultraviolet-visible (UV-Vis) spectroscopy, fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) were used for characterization. Cell stabilization membrane and lipoxidase assays were used to determine used to assess the antiinflammatory activities while 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) assays were used to assess the antioxidant activities of AgNPs [L. taraxacifolia-AgNPs, C. sesamoides Ag nanoparticles (CS-AgNPs), C. pentandra Ag nanoparticles (CP-AgNPs), and C. crepidioides AgNPs (CC-AgNPs)]. The UV-Vis spectra of the synthesized NPs displayed absorption bands at around 360-440 nm, which is a characteristic band for AgNPs. The SEM image showed that the AgNPs formed were spherical in morphology. CC-AgNPs exhibited the most significant inhibitory activity against human red blood cell membrane stabilizasyonu [median inhibitory concentration (IC50): 32.2 μg/mL] while CS-AgNPs displayed the most significant inhibitory activity against lipoxygenases (IC50: 32.8 μg/mL). CP-AgNPs exhibited the most significant antioxidant effect against both ABTS and DPPH (IC50: 5.5 and 6.4 μg/mL) when compared to ascorbic acid (IC50: 4.7 μg/mL). The synthesized AgNPs were found to be stable and the FTIR evidence suggested that the phytochemicals in the vegetables might have played an important role in the reduction and stabilization of AgNPs. This work showed that the synthesized AgNPs from non-cultivated vegetables can find relevance and application in health, drugs, food and environmental science. The evidences herein further confirmed their ethnopharmacological applications.

Nano zinc elicited biochemical characterization, nutritional assessment, antioxidant enzymes and fatty acid profiling of rapeseed.

The use of nanomaterials in agriculture is a current need and could be helpful in overcoming food security risks. Brassica napus L. is the third most important crop for edible oil, having double low unsaturated fatty acids. In the present study, we investigated the effects of green synthesized Zn NPs on biochemical effects, antioxidant enzymes, nutritional quality parameters and on the fatty acid profile of rapeseed (B. napus). Plant-mediated synthesis of zinc nanoparticles (Zn NPs) was carried out using Mentha arvensis L. leaf extract followed by characterization through ultraviolet–visible spectroscopy (UV-vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-Ray (EDX), and X-Ray diffraction (XRD). NPs exhibited irregular shapes ranging in size from 30–70 nm and EDX analysis confirmed 96.08% of Zn in the sample. The investigated biochemical characterization (protein content, proline content, total soluble sugar (TSS), total flavonoid content (TFC), and total phenolic content (TPC) showed a substantial change on exposure to Zn NPs. A dose-dependent gradual increase was observed in the antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Oil and moisture contents dropped significantly from the control level in the rapeseed (B. napus) varieties. However, different trends in nutritional (Zn, Na+, K+) and fatty acid profiling of B. napus have been noted. This study demonstrates that Zn NPs have the potential to improve the biochemical, nutritional, antioxidant enzymes, and fatty acid profile of B. napus varieties.

Biogenic silver nanoparticles induce apoptosis in Ehrlich ascites carcinoma

Introduction: Plant-mediated synthesis of silver nanoparticles (AgNPs) is accounted as an ecofriendly process. The present study was conducted to estimate the potency of biogenic AgNPs against Ehrlich ascites carcinoma (EAC) cells in vitro and EAC-bearing mice in vivo.
 Methods: AgNPs were prepared using mango leaves extract and characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), and transmission electron microscopy (TEM). Ehrlich ascites carcinoma (EAC) mouse model was established by intraperitoneal injection of 1 x 106 EAC cells. Biogenic AgNPs- alone or combined with Doxorubicin (DOX)- was administered intraperitoneally day by day for two weeks.
 Results: Biologically synthesized AgNPs showed a cytotoxic effect against cultured EAC cells but with less toxicity toward normal cells compared to DOX, which had strong cytotoxicity against both cells. Biogenic AgNPs alone or combined with DOX triggered the cytotoxicity against the EAC-bearing mouse model via decreasing body weight, tumor volume, and the number of viable tumor cells. The combined treatment (AgNPs-DOX) ameliorated the drastic effect induced by injection of EAC cells through improving liver and kidney functions compared to those treated with DOX alone. In addition, the combined treatment showed an elevation in the expression of Bax and caspase-3, and a reduction in the expression of Bcl-2 protein in the EAC cells. Furthermore, this combined treatment effectively arrested the cell cycle at the G0/G1 phase. Moreover, the combined treatment with AgNPs-DOX caused a significant reduction in the activity of ornithine decarboxylase (ODC).
 Conclusion: These findings suggest that biogenic AgNPs could be useful in developing a potent combination therapy against different types of cancers.

Phyto-Engineered Gold Nanoparticles (AuNPs) with Potential Antibacterial, Antioxidant, and Wound Healing Activities Under in vitro and in vivo Conditions.

BackgroundA diabetic ulcer is one of the major causes of illness among diabetic patients that involves severe and intractable complications associated with diabetic wounds. Hence, a suitable wound-healing agent is urgently needed at this juncture. Greener nanotechnology is a very promising and emerging technology currently employed for the development of alternative medicines. Plant-mediated synthesis of metal nanoparticles has been intensively investigated and regarded as an alternative strategy for overcoming various diseases and their secondary complications like microbial infections. Hence, we are interested in developing phyto-engineered gold nanoparticles as useful therapeutic agents for the treatment of infectious diseases and wounds effectively.Methods and ResultsWe have synthesized phyto-engineered gold nanoparticles from the aqueous extract of Acalypha indica and characterized using advanced bio-analytical techniques. The surface plasmon resonance feature and crystalline behavior of gold nanoparticles were revealed by ultraviolet-visible spectroscopy and X-ray diffraction, respectively. High-performance liquid chromatography analysis of the extract demonstrated the presence of different constituents, while major functional groups were interpreted by the Fourier-transform infrared spectroscopy as the various stretching vibrations appeared for important O-H (3443 cm−1), C=O (1644 cm−1) and C-O (1395 cm−1) groups. Scanning electron microscopy, high-resolution transmission electron microscopy results revealed a distribution of spherical and rod-like nanostructures with 20 nm of size. The gold nanoparticle-coated cotton fabric was evaluated for the antibacterial activity against Staphylococcus epidermidis and Escherichia coli bacterial strains which revealed remarkable inhibition at the zone of inhibition of 31 mm diameter against S. epidermidis. Further, antioxidant activity was tested for their free radical scavenging property, and the maximum antioxidant activity of the extract containing gold nanoparticles was found to be 80% at 100 µg/mL. The potent free radical scavenging property of the nanoparticles is observed at IC50 value 16.25 µg/mL. Moreover, in vivo wound-healing activity was carried out using BALB/c mice model with infected diabetic wounds and observed the stained microscopic images at different time intervals (day 2, day 7 and day 15). It was noted that in 15 days, the wound area is completely re-epithelialized due to the presence of different morphologies such as spherical, needle and triangle nanoparticles. The re-epithelialization layer is fully covered by nanoparticles on the wound area and also collagen filled in the scar tissue when compared with the control group.ConclusionThe pharmacological evaluation results of the study indicated an encouraging antibacterial and antioxidant activity of the greener synthesized gold nanoparticles tethered with aqueous extract of Acalypha indica. Moreover, we demonstrated enhanced in vivo wound-healing efficiency of the synthesized gold nanoparticles through the animal model. Thus, the outcome of this work revealed that the phyto-engineered gold nanoparticles could be useful for biomedical applications, especially in the development of promising antibacterial and wound-healing agents.

Green synthesis of triclinic (anorthic) phase AgCoPO4 nanoparticles: optical studies and theoretical modelling

We report the plant-mediated synthesis, structural investigation, optical properties and theoretical modelling of a triclinic (anorthic) phase AgCoPO4 nanoparticles for the first time. As part of green chemistry, the secondary metabolites in the leaf extract of Canna indica were engaged as the reducing/capping agent for the metal nanoparticles. X-ray diffraction (XRD) revealed the presence of an anorthic AgCoPO4 phase, crystallised in a triclinic structure with P -1 space group. Optical studies using UV-vis spectroscopy and photoluminescence are reported. Transmission electron microscopy suggests the formation of quasi-nanocube morphology, unlike the conventional spherically-shaped nanoparticles via plant-mediated reduction method. Elemental composition of the nanohybrid was confirmed by energy-dispersive x-ray spectroscopy (E.D.S.). Evidence of crystallinity was supported by selected area electron diffraction (SAED). Study of the dynamic anisotropy of the nanohybrid at optimised state suggests its proposed application as optical material in colourimetric metal nanoparticles-mediated sensors.

Green synthesis of nanoparticles using plant extracts: a review

Green synthesis of nanoparticles has many potential applications in environmental and biomedical fields. Green synthesis aims in particular at decreasing the usage of toxic chemicals. For instance, the use of biological materials such as plants is usually safe. Plants also contain reducing and capping agents. Here we present the principles of green chemistry, and we review plant-mediated synthesis of nanoparticles and their recent applications. Nanoparticles include gold, silver, copper, palladium, platinum, zinc oxide, and titanium dioxide.

Synthesis and Characterization of Silver Nanoparticles from <I>Ashyranthus aspera</I> Extract for Antimicrobial Activity Studies

Development of biologically inspired experimental processes for the synthesis of nanoparticles is evolving into an important branch of nanotechnology. Plant-mediated synthesis of nanomaterials has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In the present study, we were synthesized silver (Ag) nanoparticles using aqueous extracts of fresh leaves of Ashyranthus aspera medicinal plants as bio-reducing agents. UV-Vis spectrometer used to monitor the reduction of Ag ions and the formation of AgNPs in the medium. UV-Vis spectra and visual observation showed that the color of the fresh leaf extracts of Ashyranthus aspera turned into grayish-brown respectively, after treatment with Ag precursors. XRD and SEM have been used to investigate the morphology of prepared AgNPs. The peaks in the XRD pattern are associated with that of the Face-Centered-Cubic (FCC) form of metallic silver. TGA/DTA results associated with weight loss and exothermic reaction due to the desorption of chemisorbed water. FTIR was performed to identify the functional groups which form a layer covering AgNPs and stabilize the AgNPs in the medium. Moreover, silver nanoparticles using aqueous leaf extracts of Ashyranthus aspera were separately tested for their antibacterial activity against Gram-positive bacteria (Staphylococcus aureus) and Gram-negative bacteria (Enterobacter). The results showed that the bacterial growth was inhibited by the extracts containing AgNPs Nanoparticles. The biosynthesized nanoparticle was prepared from Ashyranthus aspera leaf extracts exhibits potential applications as broad-spectrum antimicrobial agents
 Keywords: Ashyranthus aspera, Silver Nanoparticles, Plant extracts, Bacteria, Antibacterial activity.

Efficacy of Methanol Leaf Extract, Biosynthesized Silver and Chitosan Nanoparticles Using Nerium oleander against Musca domestica

Plant-mediated synthesis of nanomaterials has attracted considerable attention in recent years due to its cost-effectiveness and eco-friendly nature. In the present study silver and chitosan nanoparticles were successfully synthesized using Nerium oleander leaf extract as a bio-reducing agent. Results recorded from UV–vis spectrum, HRTEM and FTIR supported the biosynthesis and characterization of silver and chitosan nanoparticles. From HRTEM analysis, the AgNPs have spherical shape morphology with an average size of about 4-32 nm. CsNPs were ranged between 34-65 nm and the shape seem spherical with dark parts confirmed the capsulated plant extract. Larvicidal activity of leaf extract of N. oleander and synthesized silver and chitosan nanoparticles was carried out against M. domestica. CsNPs showed the utmost toxicity against M. domestica after 48h exposure with LC50 value of 0.64 ppm and LC50 values of leaf extract and AgNPs were 73.024 and 2.18 ppm respectively. A significant prolongation in the larval duration was observed in treated larvae. All the tested compounds induced reduction in the pupation rate and adult emergence and caused noticeable larval, pupal, and adult abnormalities. The potency in killing M. domestica larvae and stability of N. oleander-CsNPs have made this product a good candidate for the development of a novel natural larvicide.

Role of polyphenols in plant-mediated synthesis of gold nanoparticles: identification of active components and their functional mechanism

In order to learn the active components and their functional mechanism during the plant-mediated synthesis of gold nanoparticles (GNPs), 16 kinds of plants were randomly selected as research objects, and statistical analysis was used to obtain more universal and systematic results. Polyphenols are proved to have the most prominent effect in both reductive and protective capability among all components. The relationship between polyphenols concentration and the protective and reductive capability was further studied in detail. Pyrogallic acid was used as a substitute for polyphenols to elucidate the functional mechanism. The concentration of polyphenols was found to be a critical factor for the preparation of GNPs, and the synergistic competition between phenolic hydroxyl and carbonyl groups caused by the oxidation of polyphenols could affect the particle size and morphology of GNPs. This work can provide excellent guidance for the controllable synthesis of GNPs via the plant-mediated method.

Genotoxic and Cytotoxic Activities of Lantadene A-Loaded Gold Nanoparticles (LA-AuNPS) in MCF-7 Cell Line: An in vitro Assessment.

Gold nanoparticles (AuNPs) have been widely used in many applications. Their usage as drug delivery vehicles has also gained considerable attention due to their chemical and optical properties as well as their good biocompatibility. The present study was conducted to evaluate the efficiency of AuNPs in enhancing the cytotoxic and apoptotic induction activity of lantadene A (LA), separated from Lantana camara leaves, on the breast tumor cell line MCF-7 in vitro. By utilizing plant-mediated synthesis method of nanostructures, LA-loaded AuNPs (LA-AuNPs) were prepared and their formation was confirmed by means of ultraviolet-visible spectroscope, atomic force microscope, scanning electron microscope, and zeta potential. The cytotoxic effect of LA-AuNPs was analyzed using a methylthiazol tetrazolium assay and compared to free AuNPs and LA. The results indicated a significant increase in the reduction of MCF-7 cells viability after incubation with LA-AuNPs. As determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, LA-AuNPs induced a greater ratio of DNA-fragmented cells compared to LA-treated and untreated cells. Also, by operating real-time polymerase chain reaction, LA-AuNPs-treated cells displayed an increased upregulation of p53 expression and downregulation of BCL-2 expression in addition to a significant reduction in the level of BCL-2-BAX ratio. No significant effect was shown on the expression of BAX. Collectively, our results indicate that LA-AuNPs showed promising cytotoxicity to MCF-7 cells as a novel nanoscale preparation, likely via induction of apoptotic genes and stimulation of DNA fragmentation.

Plant Mediated Synthesis Of Titanium Dioxide Nanoparticles

Plant Mediated Synthesis Of Titanium Dioxide Nanoparticles

A Study on Synthesis of Silver Nanoparticles Using Ocimum Sanctum L (Tulsi) Leaf Extract & their Antimicrobial Activity: A Green Chemistry Approach

Nanoparticles (NPs) are being widely used in different fields; therefore, there is growing interest in the development of a biological and environmental safety method for their production. Now a day’s chemical and physical methods are being used for the development of nanoparticle that is costly, time-consuming, and harmful for the environment. Plant-mediated synthesis of nanoparticles is a “Green chemistry” approach that connects different types of plants with nanotechnology. It has gained much more attention as a reliable, sustainable, and eco-friendly method for synthesizing a wide range of materials/nanomaterials. Plants are called nature’s “chemical factories” therefore, plants and plant extracts are the best options to produce different types of nanoparticles. In this present study, silver nanoparticles were synthesized by using leaf extract of Ocimum sanctum and aqueous silver nitrate solution through a simple and eco-friendly method. Then the developed silver nanoparticles were characterized by using different types of techniques such as Scanning electron microscopy (SEM), Transmission electron microscopy (TEM), UV-Vis spectrophotometer, and Fourier transforms infra-red (FTIR) spectroscopy. Their antimicrobial activity was screened against microbial culture, and it was found that the synthesized silver nanoparticles have potential applications in antibacterial activity.

Biogenic synthesis, antioxidant and antimicrobial activity of silver and manganese dioxide nanoparticles using Cussonia zuluensis Strey

Synthesis of nanoparticles using naturally occurring biomolecules has become the preferred method due to increased concerns over environmental degradation. In this study, the biosynthesis of manganese dioxide nanoparticles (MnO2NPs) and silver nanoparticles (AgNPs) using extracts and the biomolecule, aralia cerebroside, isolated from the medicinal plant species, Cussonia zuluensis Strey, was investigated. The size and morphology of nanoparticles observed using microscopic techniques indicated an average particle size of 7.43 nm (spherical and polydispersed) for AgNPs and a layer of thin film surrounding the particles, confirming the capping by biomolecules. AgNPs exhibited better antibacterial activity than MnO2NPs and were most active against Escherichia coli and Enterococcus faecalis. MnO2NPs presented as ultrathin nanoflakes with grainy morphology ranging from 11 to 29 nm when capped with biomolecules from the extract, and presented as nanospheres surrounded by nanosheets ranging from 6.99 to 16.57 nm when capped with aralia cerebroside. The radical scavenging activity was found to be MnO2NPs (extract) > MnO2NPs (cerebroside) > AgNPs (extract) > extract > cerebroside, and the ferric reducing antioxidant power was found to be cerebroside > extract > MnO2NPs (cerebroside) > MnO2NPs (extract) > AgNPs (extract). MnO2NPs exhibited better antioxidant activity than AgNPs with size and morphology of nanoparticles being influenced by the capping agent, which, in turn, influenced antioxidant activity as seen with MnO2NPs. This study confirms the significance of the metal or metal oxide core and capping biomolecules for targeted therapeutic activity of nanoparticles using the plant-mediated synthesis route.

Emerging Antineoplastic Biogenic Gold Nanomaterials for Breast Cancer Therapeutics: A Systematic Review.

Breast cancer remains as a concerning global health issue, being the second leading cause of cancer deaths among women in the United States (US) in 2019. Therefore, there is an urgent and substantial need to explore novel strategies to combat breast cancer. A potential solution may come from the use of cancer nanotechnology, an innovative field of study which investigates the potential of nanomaterials for cancer diagnosis, therapy, and theranostic applications. Consequently, the theranostic functionality of cancer nanotechnology has been gaining much attention between scientists during the past few years and is growing exponentially. The use of biosynthesized gold nanoparticles (AuNPs) has been explored as an efficient mechanism for the treatment of breast cancer. The present study supposed a global systematic review to evaluate the effectiveness of biogenic AuNPs for the treatment of breast cancer and their anticancer molecular mechanisms through in vitro studies. Online electronic databases, including Cochrane, PubMed, Scopus, Web of Science, Science Direct, ProQuest, and Embase, were searched for the articles published up to July 16, 2019. Our findings revealed that plant-mediated synthesis was the most common approach for the generation of AuNPs. Most of the studies reported spherical or nearly spherical-shaped AuNPs with a mean diameter less than 100 nm in size. A significantly larger cytotoxicity was observed when the biogenic AuNPs were tested towards breast cancer cells compared to healthy cells. Moreover, biogenic AuNPs demonstrated significant synergistic activity in combination with other anticancer drugs through in vitro studies. Although we provided strong and comprehensive preliminary in vitro data, further in vivo investigations are required to show the reliability and efficacy of these NPs in animal models.