Phytochemical Screening Studies Research Articles

Phytochemical Screening and Oral Acute Toxicity Study of Aqueous Leaf Extract of Crinum giganteum (Gadalli) in Wister Rats

<p><strong>Background:</strong> Different parts of plants possess variable phytochemical compounds and LD<sub>50</sub>. <em>Crinum giganteum</em> (Gadalli) is one of the plants most commonly consumed in parts of Africa for its various medicinal values. Despite this popularity, the phytochemical screening and the toxicity of the leaf extracts were yet to be evaluated. This study was designed to identify the bioactive agents and estimate the LD<sub>50</sub> for the aqueous leaf extract of gadalli. <strong>Method</strong>: Phytochemical screening was done using standard methods. Each bioactive agent and the LD<sub>50</sub> were estimated by Arithmetic methods of Karber. <strong>Results: </strong>Phytochemical analysis revealed high presence of alkaloids, saponins, and slight presence of glycosides while the oral LD<sub>50 </sub>was found to be 200 mg/kg. <strong>Conclusion: </strong>The major active ingredients of<strong> </strong>aqueous leaf extract of gadalli are alkaloids and saponins. It is observed to be unsafe at 200mg/kg and above.</p><p><strong>Keywords: </strong>Phytochemicals, <em>Crinum Giganteum</em>, LD<sub>50</sub>, Alkaloids, Saponins, Glycosides</p><p> </p>

Phytochemical screening, safety evaluation, anti-inflammatory and analgesic studies of the leaf extracts of Sterculia tragacantha.

Sterculia tragacantha (Sterculiaceae) is used in the treatment of boils, diarrhea, dyspepsia, fever, gonorrhea, snake bite, syphilis, and tapeworm in some West African nations. This study is to investigate its anti-inflammatory and analgesic activities since the plant is being used to treat fever. Fresh leaves of the plant were collected and dried at room temperature and pulverized into powder form and 200 g of this powder was dissolved first in hexane for 72 h and the extract was filtered and the filtrate was concentrated while the substrate was further dissolved in chloroform, ethyl acetate and methanol at different times and similar procedure adopted as for the hexane. The organic solvents were used based on order of increasing polarity. Graded concentrations of the solvent extracts were prepared and used for the study. Pilot toxicity test was carried out to determine safety dose using hematology and serum chemistry as indices of toxicity. Thereafter anti-inflammatory and analgesic studies were conducted using standard tests such as carrageenan, histamine-induced-edema, tail flick test and acetic writhing test. Phytochemical screening of the plant was also conducted. Phytochemical screening of the powdered material showed that alkaloid, flavonoid and reducing sugar were present while tannin, cardiac glycosides, saponins and anthraquinones were absent. Pilot toxicity test using aqueous extract at 100 mg/mL concentration showed that no mortality was recorded although the animals that received 3,000 mg/kg dose exhibited slight dullness after 48 h. No significant changes were also observed for the packed cell volume, hemoglobin, white blood cell counts, platelet counts, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, blood urea nitrogen, albumin, globulin except for the 200 and 3,000 mg/kg doses that caused significant increase in the level of total protein. The n-hexane, chloroform, ethyl acetate, and methanol extracts of S. tragacantha and indomethacin produced significant (p<0.05) inhibition of paw edema compared with the control using histamine and carrageenan methods of paw edema induction. There was significant (p<0.05) reduction in writhing movements at 100 mg/kg and 200 mg/kg of n-hexane, chloroform, and ethyl acetate leaf extracts of S. tragacantha and indomethacin (10 mg/kg) when compared to the control. This effect using tail flick test was not as effective when compared to the writhing test. The different leaf extracts of S. tragacantha exhibited anti-inflammatory and analgesic properties and they are also safe for medicinal use.

Phytochemical Screening and Antimicrobial Studies of the Aerial Part of Aeschynomene uniflora Mey

The aim of the study was to investigate the phytochemical composition and antimicrobial activities of extracts of aerial part of Aeschynomene uniflora Mey used in folklore medicine in order to scientifically validate some of its ethnomedicinal claims. The dried and pulverized aerial part of Aeschynomene uniflora was extracted using petroleum ether, chloroform, ethyl acetate and methanol by Soxhlet extraction and each concentrated in vacuo to yield four extracts. The extracts were then subject to preliminary phytochemical screening and antimicrobial studies using standard method. Carbohydrates, cardiac glycoside, flavonoids, saponins, steroids, triterpenes and tannins were present in the crude extracts. The highest zone of inhibition of 25 mm was exhibited by the ethyl acetated extract against Staphylococcus aureus, while the petroleum ether extract show the lowest zone of inhibition of 16 mm against Streptococcus pyogenes. The chloroform and ethyl acetate extracts exhibited a minimum inhibitory concentration (MIC) of 7 mg/mL against, Bacillus subtilis and Candida stellatoidea respectively. Both the methanol and petroleum ether extracts had a MIC of 30 mg/mL against Candida stellatoidea. The minimum bactericidal/ fungicidal concentration (MBC/MFC) of the extracts ranged between 15 mg/mL to 60 mg/mL. In conclusion, the aerial parts of Aeschynomene uniflora showed significant antimicrobial properties. This justifies the use of the in the treatment of human and animal infectious disease.

Phytochemical screening and antimicrobial potential on ethanolic extract ofGnaphalium polycaulonPers., ethnomedicinal plant used by The Niligiri tribe from Western Ghats, Tamil Nadu, India

The aim of the present study was to worked out the phytoconstituents and antimicrobial potential in ethanolic extracts of the different parts of Gnaphalium polycaulon plant. The plant material were collected and extracted with ethanol from The Nilgiri District, South India and its parts were used in folk medicine for the treatment of skin disease, respiratory problems and nervous disorders. Then it was subjected for phytochemical screening and antimicrobial study with standard procedure. The results showed that G. polycaulon exhibited the presence of major phytochemicals quantitatively and significant antimicrobial activity against all the test micro-organisms. This study evaluated significant phytoconstituents and antimicrobial activity of this ethnomedicinal plant used in folkloric medicine. Our results finding strongly supported that the crude ethanolic extracts contain medicinally important bioactive compounds and acts as antimicrobial agents.

Assessment of Quality of “Dhanyamla”: A Fermented Cereal Used in Ayurveda

Aim: Dhanyamla is a medicinal preparation having great therapeutic values which is described under the Sandhana Kalpana in Ayurveda texts. In the present study, an attempt was made to assess the quality of Dhanyamla by using standard protocols. Methodology: Quality of the Dhanyamla was assessed by determination of pH, specific gravity, microbial counts, phytochemical screening and TLC-densitogram fingerprints. Results: Specific gravity and the pH of Dhanyamla was 1.0068 and 4.13 at 30oC respectively. Pathogenic microorganisms such as Coliforms, Escherichia coli and Salmonella were not found in Dhanyamla. However, aerobic and mesophilic organisms and few counts of Staphylococcus aureus were present. Phytochemical screening studies revealed the presence of tannins, flavonoids and saponins in the sample. TLC-densitogram fingerprint of Dhanyamla was comparable to the TLC-densitogram fingerprint of its standard mixture of raw materials. Conclusion: Present study reveals the quality of Dhanyamla for the first time and observed parameters can be used as reference standard for quality control protocols in order to have a proper quality check over its preparation and processing.

Preliminary phytochemical screening, antihyperglycemic, analgesic and toxicity studies on methanolic extract of aerial parts of Corchorus olitorius L.

Antioxidant and antihyperglycemic activities of methanolic extract of Glinus oppositifolius leavesNazia Hoque, Mohammad Zafar Imam, Saleha Akter, Md. Ehsanul Hoque Mazumder, S.M. Raquibul Hasan, Jamiuddin Ahmed, Md. Sohel Rana

Phytochemical Screening and Acute Oral Toxicity Study of Java Tea Leaf Extracts.

The term Java tea refers to the decoction of Orthosiphon stamineus (OS) Benth (Lamiaceae) leaves, which are widely consumed by the people in Europe and South East Asian countries. The OS leaves are known for their use in traditional medicinal systems as a prophylactic and curative agent for urinary stone, diabetes, and hypertension and also as a diuretic agent. The present study was aimed at evaluating its possible toxicity. Herein, the major phytochemical constituents of microwave dried OS leaf, which is the common drying process for tea sachets in the market, were also identified. The acute oral toxicity test of aqueous, 50% aqueous ethanolic, and ethanolic extracts of OS was performed at a dose of 5000 mg/Kg body weight of Sprague-Dawley rats. During the 14-day study, the animals were observed for any mortality, behavioral, motor-neuronal abnormalities, body weight, and feed-water consumption pattern. The hematological and serum biochemical parameters to assess the kidney and liver functions were carried out, along with the histological analysis of these organs. It was found that all microwave dried OS leaf extracts did not cause any toxic effects or mortality at the administered dose. No abnormality was noticed in all selected parameters in rats of both sexes as compared with their respective control groups. Thus, the possible oral lethal dose for microwave dried Java tea leaves is more than 5000 mg/Kg body weight.

Phytochemical screening and antibacterial activity of medicinal plants used to treat typhoid fever in Bamboutos division, West Cameroon

This study was undertaken to document how typhoid is traditionally treated in Bamboutos division. For this purpose thirty eight plants species were selected. These plants underwent phytochemical screening and antibacterial study using standard procedures. The antibacterial tests using agar well diffusion method and microdilution assay indicated that, all the thirty eight plant samples showed activity against S. typhi, while S. paratyphi A and S. paratyphi B reacted on fifteen and fourteen plants respectively. The highest zones of inhibition were obtained from Senna alata with diameter of 24, 22.5 and 20.5 mm against S. paratyphi A, S. paratyphi B and S. typhi respectively at 160 mg/ml concentration. The lowest MIC values 128 µg/ml was exhibited by the extract of Vitex doniana against Salmonella paratyphi A. Bactericidal activity was obtained by the extract of Carica papaya, Pseudarthria confertiflora, Moringa oleifera and Harungana madagascariensis. Antibacterial screened of Pseudartthria confertiflora was reported for the first time. Annona muricata, Laggera alata, Spathodea campanulata, Cordia platythyrsa, Carica papaya, Terminalia glaucescens and Pseudarthria confertiflora gave positive results for all secondary metabolites while other plants contained two to five metabolites. The presence of these secondary metabolites probably contributes to the antibacterial potential of these plants. This finding supported the uses of these plants for treatment of typhoid fever and other infectious diseases in the study area.

Standardization of &amp;lt;i&amp;gt;Sarasvatha Choorna&amp;lt;/i&amp;gt;: Used as a Remedy for Dementia

Sri Lanka has been recognized as one of the fastest aging populations from the developing countries. There is a large senior population in the country. Since age is the biggest risk factor for Dementia, the need to create awareness and management is an increasing necessity. Sarasvatha Choorna is one of the polyherbal preparation used in Ayurveda for the management of Dementia which consists of 12 medicinal plants. In the present study, an attempt was made to standardize Sarasvatha Choorna by using standard protocols. Standardization was carried out by determination of total ash, water soluble ash, acid insoluble ash, microbial counts, heavy metals, phytochemical screening and development of TLC-densitogram fingerprints. Results revealed that 10.6±0.0% of total ash, 8.4±0.0% of water soluble ash 0.65±0.01% of acid insoluble ash 11.0±0.1% of cold ethanol extractable matter, 13.9±0.1% hot ethanol extractable matter, 13.9±0.1% cold water extractable matter and 20.2±0.2% hot water extractable matter were present in the Sarasvatha Choorna. Pathogenic microorganisms such as Coliforms, Escherichia coli and Salmonella were not found and heavy metal concentrations of Sarasvatha Choorna were well below the recommended upper limits for the tested heavy metals. Phytochemical screening studies revealed the presence of flavonoids, steroid glycosides and coumarins in both water and ethanol extracts of Sarasvatha Choorna. Presence of the raw materials in the Sarasvatha Choorna was confirmed by TLC fingerprints. Present study reveals the quality of Sarasvatha Choorna for the first time and quality control parameters resulted from this study can be used as a reference standard for quality control of Sarasvatha Choorna.

&lt;b&gt;Phytochemical Screening and Preliminary Evaluation of Analgesic and Anti-Inflammatory Activities of the Methanol Root Extract of &lt;i&gt;Cissus Polyantha&lt;/i&gt;&lt;/b&gt;

Cissus polyantha is used in traditional medicine for the treatment of conjunctivitis and inflammation. In this study, the methanolic root extract of Cissus polyantha was subjected to preliminary phytochemical screening, analgesic and anti-inflammatory studies. Phytochemical studies was carried out using standard phytochemical protocol while the analgesic studies was carried out using acetic acid-induced writhing tests in mice. Carrageenan-induced hind paw oedema in rats was used to evaluate the anti-inflammatory potential of the extract. Phytochemical studies of the methanolic crude root extract of the plant revealed the presence of carbohydrates, flavonoids, saponins, tannins steroids and triterpenes. The extract at doses of 20, 40 and 80 mg/kg, i.p significantly (P < 0.05) decreased the acetic-acid induced writhing. The extract also produced significant (P < 0.05) and dose-independent anti-inflammatory activity comparable to that of reference drug, ketoprofen. The intraperitoneal lethal dose (LD 50 ) toxicity studies on the methanol crude root extract of the plant was found to be 288.53 mg/kg body weight. These findings are suggestive of the analgesic and anti-inflammatory potentials of the methanol root bark extract of the plant and provide a scientific rationale for the use of the root of Cissus polyantha in traditional medicine. Keywords: Cissus polyantha, Phytochemical screening Analgesic, Anti-inflammatory, traditional

Phytochemical screening and anticonvulsant studies of ethyl acetate fraction of Globimetula braunii on laboratory animals

To investigate the phytochemical properties and the anticonvulsant potential of the ethyl acetate soluble fraction of ethanol leaf extract of Globimetula braunii, a plant used in ethnomedicine for the treatment of epilepsy. The phytochemical screening was carried out using standard protocol while the anticonvulsant activity was studied using maximal electroshock test in chicks, pentylenetetrazole and 4-aminopyridine-induced seizures in mice. The preliminary phytochemical screening carried out on the crude ethanol extract revealed the presence of saponins, carbohydrates, flavonoids, tannins, anthraquinones and steroids. Similarly, tannins, flavonoids and steroids/terpenes were found to be present in the ethyl acetate fraction. In the pharmacological screening, 150 mg/kg of the fraction protected 83.33% of animals against pentylenetetrazole-induced seizure in mice whereas sodium valproate a standard anti-epileptic drug offered 100% protection. In the 4-aminopyridine-induced seizure model, the fraction produced a significant (P<0.05) increase in the mean onset of seizure in unprotected animals. The fraction did not exhibit a significant activity against maximal electroshock convulsion. The median lethal dose of the fraction was found to be 1 261.91 mg/kg. These results suggest that the ethyl acetate fraction of Globimetula braunii leaves extract possesses psychoactive compound that may be useful in the management of petit mal epilepsy and lend credence to the ethnomedical use of the plant in the management of epilepsy.

Analysis of phytochemical constituents of the ethanolic and chloroform extracts of Calotropis procera using gas chromatography-mass spectroscopy (GC-MS) technique

The phyto-components of Calotropis procera leaves were screened by qualitative and gas chromatography-mass spectroscopy (GC-MS) analysis. C. procera R.Br. is a perennial plant abundantly found in all parts of the country (India) and wild in nature. The leaves of the plant were found to contain various primary and secondary metabolites. Qualitative analysis showed the presence of alkaloids, terpenoids, saponins, tannins and cardiac glycosides and absence of flavonoids in ethanolic extract of C. procera, while the chloroform leaf extract showed absence of flavonoids and cardiac glycosides. This work deals with the phytochemical screening and GC-MS studies of the ethanolic and chloroform leaf extracts of C. procera. In ethanol leaf extract, highest peak area (%) of 21.36 was obtained by 9-octadecenoic acid (Z), methyl ester (C19H36O2) at retention time of 14.843 and the lowest peak area (%) of 0.31 was obtained by cyclohexanol-3-methyl (C7H14O) at retention time of 18.599, whereas in chloroform leaf extract, the highest peak area (%) of 33.14 was obtained by 2, 6, 10-trimethyl, 14-ethylene-14-pentadecne (C20H38) at retention time of 13.136 and the lowest peak area (%) of 0.78 was obtained by 2-tert-butyl-4-(1,1,3,3-tetramethylbutyl) C18H30O at retention time of 10.731. The study summarizes the information concerning the phytochemical constituents present in ethanolic and chloroform leaf extracts. These constituents may be responsible for pharmacological activities. Key words: Calotropis procera, phytochemical analysis, GC-MS, metabolites, leaves.

Phytochemical identification of Nilgirianthus ciliatus by GC-MS analysis and its DNA protective effect in cultured lymphocytes

Aim: To identify the phytochemical constituents and antioxidant activities of ethanol leaf extract of Nilgirianthus ciliatus (NC) and its protective effect against H2O2-induced DNA damages in cultured lymphocytes. Materials and Methods: The fresh leaves of NC (1000g) were shade dried at room temperature for 30 days and the dried leaves was made into a fine powder. The ethanol leaf extract obtained was dried and used for phytochemical identification by GC-MS analysis, in vitro radical scavenging assays and DNA protection in lymphocytes. Results and Discussions: The phytochemical screening studies identified sixteen chemical constituents present in the leaf extract of NC. The ethanol extract of NC (20, 40, 60, 80, 100 μg/ml) exhibited a significant dose dependent inhibition of in vitro radical scavenging assays and their corresponding IC50 values as follows such as Superoxide anion (32.00 ± 0.33 μg/ml) and DPPH radical (47.11 ± 0.50 μg/ml ). The DNA protection was observed at 60 μg/ml of NC against 500 μM H2O2 treated lymphocytes. Conclusion: Thus, our results show that NC exhibits in vitro radiacal scavenging assays and offers protection against H2O2-induced DNA damage in cultured lymphocytes and it can be developed as an effective antioxidant during oxidative stress. Keywords: Nilgiranthus

Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris

ObjectiveTo prepare various crude extracts using different polarities of solvent and to quantitatively evaluate their total phenol, flavonoids contents and phytochemical screening of Thymus vulgaris collected from Al Jabal Al Akhdar, Nizwa, Sultanate of Oman. MethodsThe leave sample was extracted with methanol and evaporated. Then it was defatted with water and extracted with different polarities organic solvents with increasing polarities. The prepare hexane, chloroform, ethyl acetate, butanol and methanol crude extracts were used for their evaluation of total phenol, flavonoids contents and phytochemical screening study. The established conventional methods were used for quantitative determination of total phenol, flavonoids contents and phytochemical screening. ResultsPhytochemical screening for various crude extracts were tested and shown positive result for flavonoids, saponins and steroids compounds. The result for total phenol content was the highest in butanol and the lowest in methanol crude extract whereas the total flavonoids contents was the highest in methanol and the lowest hexane crude extract. ConclusionsThe crude extracts from locally grown Thymus vulgaris showed high concentration of flavonoids and it could be used as antibiotics for different curable and uncurable diseases.

Anti–ulcer activity of Ficus religiosa leaf ethanolic extract

ObjectiveTo evaluate the anti-ulcer activity and acute toxicity of Ficus religiosa (F. religiosa) leaf ethanolic extract in animal models. MethodsAnti-ulcer activity of F. religiosa ethanolic extract (250 and 500 mg/kg body weight) was studied on stress induced ulcer animal models. Ranitidine was used as standard. The anti-ulcer activity of F. religiosa was evaluated with the help of ulcer area and histopatholgical examination. Preliminary phyto-chemical screening and acute toxicity studies of F. religiosa also carried out. ResultsResults showed that the extract treatments prevented ulcer area and gastric secretion in a dose-dependent manner. Administration of 2 000 mg/kg extract did not show any acute toxicity in albino mice. Preliminary phytochemical analysis identified the presence of flavonoids in the ethanolic extract of F. religiosa. ConclusionsThe extract is non-toxic even at relatively high concentrations. The anti-ulcer activity is probably due to the presence of flavanoids.

Phytochemical investigation and radical scavenging activities of Melia azedarach and its DNA protective effect in cultured lymphocytes

Context: Melia azedarach Linn (Meliaceae) is an Ayurvedic medicinal plant which is native to India. It is traditionally used for the treatment of leprosy, inflammation, scrofula, anthelmintic, antilithic, diuretic, deobstruent and cardiac disorders.Objective: To evaluate the phytochemical constituents and antioxidant activities of the ethanol leaf extract of Melia azedarach (MA) and its protective effect against H2O2-induced cellular damage in cultured lymphocytes.Materials and methods: The dose-dependent study of MA (20, 40, 60, 80, 100 µg/ml) was used to study in vitro radical scavenging assays. The effective dose of MA (60 µg/ml) was further used to study the H2O2-induced DNA damage (comet assay and DNA fragmentation assay) in cultured lymphocytes.Results: The ethanol extract of MA (20, 40, 60, 80, 100 µg/ml) exhibited a significant dose-dependent inhibition of in vitro radical scavenging assays and their corresponding IC50 values as follows: hydroxyl radical (26.50 ± 0.26 µg/ml), superoxide anion (30.00 ± 0.32 µg/ml), nitric oxide radical (48.00 ± 0.48 µg/ml), DPPH radical (30.55 ± 0.32 µg/ml) and reducing power (22.00 ± 0.22 µg/ml). The increase in the severity of DNA damage and TBARS was increased significantly (p < 0.05) at 500 µM H2O2-treated cultured lymphocytes and RBC cellular membranes. The phytochemical screening studies identified 13 chemical constituents present in the leaf extract of MA.Discussion and conclusion: The results of this study demonstrate that MA offers protection against H2O2-induced cellular damage and it can be developed as an effective antioxidant during oxidative stress.

Phytochemical screening and antimicrobial studies on the methanolic bulb extract of Allium sativum L.

Methanolic extract of the bulb of Allium sativum was screened for phytochemical and antimicrobial properties. The crude extract of A. sativum bulb showed positive results for carbohydrates, glycosides and proteins in high concentrations; alkaloids, saponins, reducing sugars, oils and steroids in medium concentrations, while flavonoids and acidic compounds were present in low amounts. Tannins, resins and terpenoids were however absent in the extract. All the six tested microorganisms exhibited varied susceptibility to the plant extract. The minimum inhibitory concentration (MIC) of the organisms were, 50 mg/ml for Salmonella paratyphi, 25 mg/ml for Bacillus subtilis, 100 mg/ml for Klebsiella pneumoniae, 12.5 mg/ml for Candida albicans, 3.12 mg/mlfor Candida paralopsis and 6.25 mg/ml for Candida tropicalis. A. sativum showed both antifungal and antibacterial properties. Key words: Phytochemical screening, antimicrobial activity, Allium sativum, bulb extract.

Phytochemical screening and toxicity studies on the methanol extract of the seeds of moringa oleifera

The seeds of Moringa oleifera were collected, air-dried, pulverized, and subjected to cold extraction with methanol. The methanol extract was screened phytochemically for its chemical components and used for acute and sub-acute toxicity studies in rats. The phytochemical screening revealed the presence of saponins, tannins, terpenes, alkaloids, flavonoids, carbohydrates, and cardiac glycosides but the absence of anthraquinones. Although signs of acute toxicity were observed at a dose of 4,000 mg kg-1 in the acute toxicity test, and mortality was recorded at 5,000 mg kg-1, no adverse effect was observed at concentrations lower than 3,000 mg kg-1. The median lethal dose of the extract in rat was 3,873 mg kg-1. Sub-acute administration of the seed extract caused significant (p<0.05) increase in the levels of alanine and aspartate transferases (ALT and AST), and significant (p<0.05) decrease in weight of experimental rats, at 1,600 mg kg-1. The study concludes that the extract of seeds of M. oleifera is safe both for medicinal and nutritional uses.

Phytochemical screening studies on Melia orientalis by GC-MS analysis

Background:Melia orientalis (MO) is an important Ayurvedic medicinal plants. The plant part such as leaves and roots are traditionally used for the treatment of diabetes, edema, traumatic swelling, skin diseases, oligospermia and bleeding disorders.Objective:To investigate the phytochemical identification of ethanol leaf extract of MO.Materials and Methods:The fresh leaves of MO (1000g) were collected and shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanol leaf extract obtained was dried and used for phytochemical identification by GC-MS analysis.Results:The phytochemical screening studies have been carried out and identified ten chemical constituents present in the leaf extract of MO.Conclusion:Thus, our results show that MO possess important phytocomponents such as phytol, squalene and stigmasterol.

Investigation of antioxidant activity and phytochemical constituents of Artocarpus altilis

The phytochemical screening study and the antioxidant activity test of the twigs ofArtocarpus altilis has been done for the first time. The Artocarpus genus is known to produce a large number of secondary metabolites, and is specifically rich in phenylpropanoids such as flavonoids and flavones. Results of this study indicated that A. altilis (twigs) contain terpenoids, saponins, phenolic group, flavonoids, glycoside, steroids and tannins, while alkaloid test yielded negative result. Investigation on these chemical compounds was done using the standard technique for assay of phytochemical constituents, with modification. The dried plant was extracted by using soxhlet apparatus using three solvents namely hexane, dichloromethane (DCM) and methanol, and it was observed that the plant has a characteristic of antioxidant using DPPH assay. DCM extract of Artocarpus altilis has a remarkable antioxidant activity with an IC50 value of 0.015 mg/ml compared with the standard butylated hydroxytoluene (BHT). This finding supports the possibility of using this plant for medicinal treatments. Key words: Phytochemical, antioxidant, 1,1-diphenyl-2-picrylhydrazyl (DPPH), Artocarpus, medical treatment.