Phytochemical identification of Nilgirianthus ciliatus by GC-MS analysis and its DNA protective effect in cultured lymphocytes

Abstract

Aim: To identify the phytochemical constituents and antioxidant activities of ethanol leaf extract of Nilgirianthus ciliatus (NC) and its protective effect against H2O2-induced DNA damages in cultured lymphocytes. Materials and Methods: The fresh leaves of NC (1000g) were shade dried at room temperature for 30 days and the dried leaves was made into a fine powder. The ethanol leaf extract obtained was dried and used for phytochemical identification by GC-MS analysis, in vitro radical scavenging assays and DNA protection in lymphocytes. Results and Discussions: The phytochemical screening studies identified sixteen chemical constituents present in the leaf extract of NC. The ethanol extract of NC (20, 40, 60, 80, 100 μg/ml) exhibited a significant dose dependent inhibition of in vitro radical scavenging assays and their corresponding IC50 values as follows such as Superoxide anion (32.00 ± 0.33 μg/ml) and DPPH radical (47.11 ± 0.50 μg/ml ). The DNA protection was observed at 60 μg/ml of NC against 500 μM H2O2 treated lymphocytes. Conclusion: Thus, our results show that NC exhibits in vitro radiacal scavenging assays and offers protection against H2O2-induced DNA damage in cultured lymphocytes and it can be developed as an effective antioxidant during oxidative stress. Keywords: Nilgiranthus