Phosphorus Cycling Research Articles

Potential interaction of soybean-grass intercropping with residual nitrogen for a no-tillage system implementation

Combinations between crop intercropping and forage production in a no-tillage system are not well established for environments under low water retention and soil fertility conditions. Our study aimed to evaluate the potential interactions between soybean-grass intercropping with residual nitrogen in a no-tillage system. The experimental design was a randomized block with four replications in a subdivided plot scheme. The main plots in the summer season were: 1) soybean monoculture; 2) soybean - Aruana Guinea grass (Megathyrsus maximus cv. Aruana) intercropping, and 3) soybean - Congo grass (Urochloa ruziziensis cv. Comum) intercropping. The subplots were the nitrogen rates of 0, 50, 100, and 150 kg ha-1, applied as side-dressing in maize and grasses during the autumn-winter season. Here, the results of the summer seasons are shown. To do so, the parameters evaluated were soybean agronomic traits, dry biomass production, and macronutrient concentrations of plants intercropped. The main effects and interactions were studied. Our findings showed that soybean-Aruana Guinea grass intercropping is an alternative to no-tillage system implementation. Moreover, residual nitrogen from the autumn-winter season directly interfered with the dry biomass production of grasses intercropped with soybeans in the summer season. In short, the systems studied seem suitable for implementing a no-tillage system, which aims to neutralize degraded pastures and produce forage for ensilage. Despite the changes in macronutrient concentrations within the intercropping system and residual nitrogen, and among intercropped crops over the years, Congo grass intercropped with soybeans in the summer season showed great capacity for phosphorus, potassium, and magnesium cycling.

Machine learning modeling for the prediction of phosphorus and nitrogen removal efficiency and screening of crucial microorganisms in wastewater treatment plants

The effectiveness of wastewater treatment plants (WWTPs) is largely determined by the microbial community structure in their activated sludge (AS). Interactions among microbial communities in AS systems and their indirect effects on water quality changes are crucial for WWTP performance. However, there is currently no quantitative method to evaluate the contribution of microorganisms to the operating efficiency of WWTPs. Traditional assessments of WWTP performance are limited by experimental conditions, methods, and other factors, resulting in increased costs and experimental pollutants. Therefore, an effective method is needed to predict WWTP efficiency based on AS community structure and quantitatively evaluate the contribution of microorganisms in the AS system. This study evaluated and compared microbial communities and water quality changes from WWTPs worldwide by meta-analysis of published high-throughput sequencing data. Six machine learning (ML) models were utilized to predict the efficiency of phosphorus and nitrogen removal in WWTPs; among them, XGBoost showed the highest prediction accuracy. Cross-entropy was used to screen the crucial microorganisms related to phosphorus and nitrogen removal efficiency, and the modeling confirmed the reasonableness of the results. Thirteen genera with nitrogen and phosphorus cycling pathways obtained from the screening were considered highly appropriate for the simultaneous removal of phosphorus and nitrogen. The results showed that the microbes Haliangium, Vicinamibacteraceae, Tolumonas, and SWB02 are potentially crucial for phosphorus and nitrogen removal, as they may be involved in the process of phosphorus and nitrogen removal in sewage treatment plants. Overall, these findings have deepened our understanding of the relationship between microbial community structure and performance of WWTPs, indicating that microbial data should play a critical role in the future design of sewage treatment plants. The ML model of this study can efficiently screen crucial microbes associated with WWTP system performance, and it is promising for the discovery of potential microbial metabolic pathways.

The Impact of Phosphorus Level on Vivianite Precipitation during Microbial Reduction of Ferrihydrite

Iron minerals play a pervasive role in the cycling of phosphorus (P) within both terrestrial and aquatic environments. The behavior of P, especially in oxygen-depleted environments, is frequently regulated by changing redox conditions and the associated phase transformations of Fe (III) (hydr)oxides (Borch and Fendorf 2007). Although the stability of Fe (III) hydroxides under changing redox conditions is well established, the relationship between specific minerals and their influence on the mechanisms of P retention and release remain unclear. In particular, the minerals vivianite (Fe3(PO4)2·8H2O) and ferrihydrite (Fe2O3·0.5H2O) are of interest. Vivianite crystallization in sediments has attracted increasing attention as a major contributor to P retention during early diagenesis (Slomp et al. 2013) and ferrihydrite reduction has been linked to fluctuating P concentrations which can impact vivianite crystallization. Additionally, there exists strong biological controls on these minerals as the reduction of Fe (III) oxides by dissimilatory iron-reducing bacteria may result in the formation of a suite of Fe (II)-bearing secondary minerals (O’Loughlin et al. 2021). To better understand the biogeochemical mechanisms behind these interactions, we examined the effects of fluctuating P concentrations on the reduction of ferrihydrite by Shewanella putrefaciens CN32 and resulting vivianite formation. In this study, bio-reduction experiments were conducted under sterile conditions in serum bottles containing 80 mL of mineral medium, with 80 mM Fe (III) in the form of Ferrihydrite, and various P concentrations (0, 1 mM, and 10 mM). The bottles were placed on a roller drum (180 rpm) and incubated at 30 oC in the dark for 15 days. Dissolved Fe (II), P concentrations, pH, and optical density (OD) values throughout the experiments were also measured. Our results showed that during incubation, Shewanella putrefaciens CN32 accelerated Ferrihydrite reduction as the dissolved iron (Fe2+) concentration increased significantly when compared to other bacterial and ferrihydrite treatments, as well as the control treatment. Additionally, P in both the 1mM and 10mM treatments was depleted after 5 and 7 days, respectively, and resulted in crystalline precipitates. Scanning electron microscopy (SEM) analyses of precipitates showed well-formed and highly crystalline vivianite particles of up to 30 µm in length in high P level. Some crystalline precipitates were confirmed as vivianite through SEM and Raman spectroscopy matching a known vivianite reference (Taylor et al. 2008), but only within the 10mM P incubations. After 15 days of incubation the morphology of the vivianite changed from aggregates of lath-shaped crystals to acicular crystals (Fig. 1). Based on our results, fluctuating P concentrations do indeed have a pronounced effect on ferrihydrite reduction and thus vivianite formation.

Phosphorus and Lake Eutrophication: Recent Findings and Emerging Challenges

This presentation reviews some recent and ongoing research on phosphorus (P) cycling in a variety of lake systems. Phosphorus is an essential nutrient element, and its anthropogenic enrichment is generally considered to be the main driver of cultural eutrophication of freshwater lakes, which, in the worst case, leads to the occurrence of harmful algal blooms, the intensification of hypoxia and the die-off of aquatic life. The research presented shows that excess external P loading causes the accumulation of reactive chemical P forms in the bottom sediments of lakes (Update #1). The slow release of this reactive legacy P back to the water column can significantly delay a lake’s recovery following the reduction of external P loading (Update #2). Land use changes accompanying agricultural intensification and urbanization generally increase P emissions, but the implementation of agricultural and stormwater best management practices can effectively mitigate external P loads to receiving lakes (Update #3). However, additional stressors, including climate change and salinization, magnify in-lake P mobilization pathways and, hence, increase the risks of lake (re-)eutrophication (Update #4).

Unraveling the rate-limiting step in microorganisms' mediation of denitrification and phosphorus absorption/transport processes in a highly regulated river-lake system.

River-lake ecosystems are indispensable hubs for water transfers and flow regulation engineering, which have frequent and complex artificial hydrological regulation processes, and the water quality is often unstable. Microorganisms usually affect these systems by driving the nutrient cycling process. Thus, understanding the key biochemical rate-limiting steps under highly regulated conditions was critical for the water quality stability of river-lake ecosystems. This study investigated how the key microorganisms and genes involving nitrogen and phosphorus cycling contributed to the stability of water by combining 16S rRNA and metagenomic sequencing using the Dongping river-lake system as the case study. The results showed that nitrogen and phosphorus concentrations were significantly lower in lake zones than in river inflow and outflow zones (p < 0.05). Pseudomonas, Acinetobacter, and Microbacterium were the key microorganisms associated with nitrate and phosphate removal. These microorganisms contributed to key genes that promote denitrification (nirB/narG/narH/nasA) and phosphorus absorption and transport (pstA/pstB/pstC/pstS). Partial least squares path modeling (PLS-PM) revealed that environmental factors (especially flow velocity and COD concentration) have a significant negative effect on the key microbial abundance (p < 0.001). Our study provides theoretical support for the effective management and protection of water transfer and the regulation function of the river-lake system.

Unveiling the ecological significance of algal mats and meadows: Insights into phosphorus cycling and primary production of benthic algae in post-mining lakes

Post-mining lakes are anthropogenic water bodies increasing globally due to mining suppression and recultivation. These lakes have ecological and socioeconomic significance, providing freshwater sources, wildlife habitats, and recreation potential. However, research on primary producers in these lakes is limited, with most studies focusing on phytoplankton while neglecting algal mats and meadows. If developed in high biomass, algal mats and macroalgal meadows play a crucial role in aquatic ecosystems. They contribute to the trophic structure, nutrient fluxes, primary production and to the purification of entire waterbodies. Yet, there is a lack of knowledge regarding the nutrient acquisition and the contribution of algal mats and meadows to total primary production and phosphorus (P) cycling, especially in oligotrophic lakes, including post-mining lakes. Our team investigates the benthic algal primary producers - algal mats and meadows in the littoral zone of tree newly established post-mining lakes located in the Czech Republic (Europe). Our main objectives are to describe the quality and quantity of primary producers, quantify the contribution of algal mats and meadows to the primary production and phosphorus cycling in the oligotrophic post-mining lakes. We have studied them throughout all seasons with a special focus on the generally neglected winter/spring season. Our sampling strategy encompasses three gradients of variability along which the composition and ecological role of benthic algae change: spatial variability along the depth gradient within each lake, itemporal variability throughout the seasons within each lake and successional variability between the lakes of different age. spatial variability along the depth gradient within each lake, itemporal variability throughout the seasons within each lake and successional variability between the lakes of different age. The methodological approach consists of measuring the limnological and chemical parameters of lakes, taxonomic determination, biomass estimation and C:N:P stoichiometry of algal biomass along the whole gradient of the euphotic zone (0-15m). The in situ measurement of primary production of benthic algae from various depths using the oxygen meter Fibox 3 (PreSens, Germany) with a combination of laboratory experiments was employed too (Čapková 2022). We measure the kinetic parameters of P uptake of algal mats and meadows using 33P-labelled orthophosphates under controlled conditions similar to the nature (Konopáčová 2021). According to our survey, algal mats and meadows exhibited high productivity in the entire littoral zone. Algal mats dominate the depth up to 3m Fig. 1 and are highly diverse, with more than 400 identified taxa (Bešta 2022). Macroalgal meadows formed by the genus Chara together with the genus Vaucheria, are key taxa occupying the deeper zone (3-15m) Fig. 2. The distribution of benthic algae in lakes is influenced by depth gradients, substrate quality, seasonality and age of lakes foundation, resulting in complex spatio-temporal patterns. Specific emphasis is placed on the fate of P, which is the limiting nutrient in investigated lakes. High C:P molar ratios in microalgal mats indicate strong P deficiency in studied lakes. Over the vegetation season, microalgal biomass doubled, while the P content in biomass dropped to 60% of the values from the start of the vegetation season. The maximum uptake velocity and specific P uptake affinity decreased by an order of magnitude from April to October, and P uptake affinity was measured for comparison in plankton too. Based on our results, we proposed a possible mechanism underlying a stable coexistence of planktonic and benthic primary producers, with plankton prospering primarily in summer and autumn and algal mats and meadows in winter and spring seasons. By integrating data collected during five years of investigation, our study aims to provide a comprehensive understanding of the ecological roles of primary producers in post-mining lakes.

Defoliation enhances the positive effects of soil microbial diversity on plant productivity

Abstract Defoliation, often induced by herbivores, and soil microbiomes are recognized as key drivers of plant diversity and productivity in grasslands. However, lack of experiments simultaneously manipulating defoliation and soil microbial diversity has constrained our understanding of how these two factors interactively influence both above‐ground and below‐ground net primary productivity (ANPP and BNPP). We employed a soil filtering approach to create the gradient of soil microbial diversity and removal of above‐ground biomass by clipping to simulate defoliation effect from herbivores. Our goal was to investigate how soil microbial diversity affected both ANPP and BNPP under the stress of defoliation. We found that defoliation and soil microbial diversity loss decreased the ANPP and BNPP of the plant community, and Medicago falcata (legume) was more responsive to these treatments than Leymus chinensis (grass) and Artemisia frigida (forb). ANPP was positively correlated with soil microbial richness only when plants suffered defoliation. By contrast, BNPP showed a closer relationship with soil fungal richness than bacteria, and defoliation enhanced this relationship. The structural equation model (SEM) showed that defoliation indirectly regulated ANPP by affecting plant P content, while soil bacterial diversity loss decreased the ANPP through altering plant P and fungal diversity loss directly decreased BNPP. The LEfSe and linear regression analysis revealed that the biomarkers of bacteria (e.g. Actinobacteria, Xanthobacteraceae) and fungi (e.g. Ascomycota, Glomerales) significantly contributed to the variation of ANPP and BNPP under defoliation treatment respectively. Defoliation enhanced phosphorus acquisition by soil microorganisms, further strengthening the microbial regulation of plant productivity. Overall, we provide novel insights on how defoliation enhances the positive effects of soil microbial diversity on above‐ and below‐ground net primary productivity. Our findings highlight that soil microorganisms associated with phosphorus cycling are mobilized to play a more active role in promoting productivity after defoliation. It may provide a new perspective on how to better utilize the role of soil microorganisms in grazed or managed grasslands. Read the free Plain Language Summary for this article on the Journal blog.

Widespread marine euxinia along the western Yangtze Platform caused by oxygen minimum zone expansion during the Capitanian mass extinction

The development of widespread marine anoxic and/or euxinic conditions has been proposed as a likely driver of the mid-Capitanian mass extinction. However, the driving mechanisms and spatiotemporal evolution of anoxia/euxinia remain poorly constrained. In order to decipher changes in marine redox conditions and their possible influence on the mid-Capitanian biotic crisis, we applied multiple geochemical indicators to three sections across a shelf-to-basin transect in the Middle Permian Kuhfeng and Lower Yinping formations of the Lower Yangtze Basin, South China. Our integrated Fe speciation and redox-sensitive trace element data suggest that euxinic waters dynamically coexisted at intermediate depths on the western margin of the Yangtze Platform, with oxygenated surface waters and ferruginous deeper waters providing compelling evidence for a redox structure similar to an oxygen minimum zone (OMZ). The synthesis of a five-stage spatiotemporal evolution of redox conditions, coupled with changes in upwelling and hydrographic restriction, indicates particularly intensified euxinia and an expanded OMZ across the shelf-basin transect during the middle Capitanian. Long-lasting anoxia-euxinia was likely maintained by enhanced phosphorus cycling, with sluggish ocean circulation due to collapsed upwelling and enhanced restriction under climate warming intensifying the euxinic conditions. Through a comparison of available global data, we infer that widespread anoxia-euxinia was prevalent in the middle Capitanian due to expanded OMZ conditions. Expanded anoxia and euxinia in shelf and slope environments occurred concurrently with an ongoing biotic crisis suggesting that these redox changes were a contributory factor.

Evaluation of the impact of aquatic-animal excretion on nutrient recycling and retention in stormwater treatment wetlands

The relative contribution of aquatic animals to phosphorus (P) and nitrogen (N) cycles of water bodies can be substantial. Excretion rates can be affected by seasonal environmental conditions, physiology, and body size. Determining the importance of particular species or size classes to total fauna-driven internal recycling of nutrients requires measurements of seasonal mass-specific excretion rates (MSER). We used short-term incubation experiments to estimate the contribution of excretion and egestion by eight species of fish and one macroinvertebrate to water column P and N in Stormwater Treatment Areas (STAs) used to retain P from water flowing to the Everglades in Florida, U.S.A. To account for environmental conditions and fish physiology, excretion was estimated during day and night in the wet and dry seasons for nine of the most abundant fish and invertebrates found in the STAs. To yield measurable contributions of analytes in short-duration incubations, large-fish species were incubated individually, whereas small-fish species and invertebrates were incubated in single-species groups. For large-fish species, total excreted nutrients were positively correlated with the mass of individual fish, but MSER decreased with increased individual size. All four small species, three fish and invertebrate, excreted P and N at greater MSERs than all five large-fish species. Most MSERs were not correlated with incubated mass (stocked density) of small animals (80%), indicating that there were no density-dependent effects on excretion rates. Most species excreted more N and less P during the South Florida wet season (May to October) though this was not the case for one or more analytes for three of the large species. Most species excreted more during the day, except large herbivores that excreted more at night. Small species excreted higher proportions of particulate P and N, while the larger species had higher proportions of dissolved nutrients. Using measured MSERs and areal biomass estimates for the nine species, we estimated mass of P and N excreted annually. Based on known inflow P and N loads for four STAs, a preliminary and conservative estimate is that fish excretion can recycle between 50% to 92% of the annual total P entering the system and 15% to 29% of the annual total N entering the system. These preliminary results underestimate the actual amounts because they do not account for STA cells that were not sampled, nor known under-estimation of large-fish biomass, as well as other animal effects on nutrient cycles such as bioturbation. Though preliminary, our results suggest that altering aquatic animal abundance or species composition could improve the efficiency of STAs to remove and retain nutrients from inflowing waters.

Exploring the mechanism and negentropy of photogranules for efficient carbon, nitrogen and phosphorus recovery from wastewater

Entropy is a fundamental concept in thermodynamics that characterizes the level of disorder or chaos within a system. Negative entropy is the process of fixing unavailable disordered energy into available ordered energy, which can serve as a valuable indicator of the sustainability and efficiency of wastewater treatment process. This study explored the utilization of photogranules (light-driven aerobic granular sludge) as a wastewater treatment process with minimal energy dissipation and effective resource recovery. This study focuses on the carbon, nitrogen, and phosphorus cycling pathways of photogranules, including treatment performance, resource recovery, neg-entropy evaluation (NEE), and their corresponding metabolic mechanisms. Photogranules showed 94.1 % COD, 96.1 % ammonium and 88.9 % phosphorus removal ratios, respectively. Moreover, the successful recovery of proteins 337.61 mg/g, polysaccharides 87.67 mg/g, lipid 206.6 mg/g, which achieved negentropy. Notably, the microorganisms Scenedesmus, responsible for carbon fixation, and Allorhizobium, responsible for nitrogen fixation, play pivotal roles in driving the negentropy process. During carbon fixation, light energy is converted into chemical energy through the process of photosynthesis, The dark reaction phase of photosynthesis involves the production of glucose, and it has been found to have a thermodynamic value of −14134.8 J/(mol·K), indicating a decrease in entropy. Thermodynamic modeling further supports the concept that photogranules achieve negentropy. They accomplish this by effectively sequestering and converting non-renewable light resources into a state of low entropy. In summary, NEE provides a novel insight into not only the sustainability of photogranules but also other wastewater treatment process.

Enhanced chemical recovery of phosphorus from residues of recirculating aquaculture systems (RAS)

In recirculating aquaculture systems, cultivated fish cannot incorporate all the phosphate contained in the feed. Excess phosphate ends up in the culture water and in the sludge produced. If the sludge cannot be valorised directly in agriculture, a chemical recovery as concentrated phosphate is an interesting option to help closing the phosphorus cycle. This study investigates the extent to which accumulated phosphorus can be recovered by acid re-dissolution with subsequent precipitation on sludge from two different recirculating aquaculture systems cultivating African Catfish (Clarias gariepinus). Acid treatment could increase the ratio of dissolved phosphorus available for subsequent precipitation by 53% for extensive and by 61% for intensive fish farming. With the consecutive precipitation in total up to 86% of the phosphorus from the sludge could be recovered. Phosphorus re-dissolution with citric acid requires the highest amount of acid per g dry matter as well as of sodium hydroxide for subsequent precipitation. Sulfuric and nitric acids have comparably lower demands.

Mechanisms of soil respiration and its temperature sensitivity in black soil farmland

Soil respiration is the process of decomposition of organic matter in soil and microbial metabolism to produce carbon dioxide. In the context of climate warming and degradation of agricultural soil fertility, it becomes crucial to explore the mechanisms of soil respiration to increase carbon sequestration. In this paper, five biochar applications (0, 3.79, 7.58, 11.36, 15.15 t/ha) and two tillage methods (shallow ploughing, deep ploughing) were set up to explore the mechanism of soil respiration and its temperature sensitivity by studying the effects of tillage methods and different amounts of biochar application on soil properties, soil enzyme activities, soil respiration and its temperature sensitivity during the growing season of soybeans in the black soil area. The results showed that soil respiration rate increased with biochar application in the low biochar application range; the opposite trend was observed for high biochar application beyond the threshold (11.36 t/ha). The application of 11.36 t/ha of biochar amount had the strongest promotion effect on soil respiration, reaching 2.42 μmol m−2·s−1 in the shallow ploughing treatment and increasing 0.32 μmol m−2·s−1 in the deep ploughing compared to the shallow ploughing. The application of biochar significantly increased the activity of nitrogen and phosphorus enzymes and had an inhibitory effect on carbon cycle enzymes; the increased activity of nitrogen and phosphorus cycle enzymes provided sufficient substrate for microbial respiration and thus increased soil respiration, and the decrease in the enzymatic reaction of carbon cycle enzymes led to a decrease in temperature sensitivity. The temperature sensitivity of deep ploughing (2.18–2.59) got a more substantial reduction than that of shallow ploughing (2.34–3.53), which indicates that deep ploughing treatments can better reduce the mineralization of organic carbon than shallow tillage. Meanwhile, the temperature sensitivity of soil respiration reached a minimum with the application of 7.58 t/ha of biochar, which was reduced by 16% under deep ploughing treatment compared to the control, 33% under shallow ploughing treatment compared to the control, and also reduced by 27.27% under deep ploughing compared to shallow ploughing, which suggests that the addition of biochar reduces the loss of carbon to a certain extent. This study is important for assessing the effects of tillage practices and biochar combinations on carbon sequestration in agricultural soils in black soil areas.

Transcriptome Analysis Reveals Candidate Genes Involved in Gibberellin-Induced Fruit Development in Rosa roxburghii.

Gibberellins (GAs) play indispensable roles in the fruit development of horticultural plants. Unfortunately, the molecular basis behind GAs regulating fruit development in R. roxburghii remains obscure. Here, GA3 spraying to R. roxburghii 'Guinong 5' at full-bloom promoted fruit size and weight, prickle development, seed abortion, ascorbic acid accumulation, and reduction in total soluble sugar. RNA-Seq analysis was conducted to generate 45.75 Gb clean reads from GA3- and non-treated fruits at 120 days after pollination. We obtained 4275 unigenes belonging to differently expressed genes (DEGs). Gene ontology and the Kyoto Encyclopedia of Genes and Genomes displayed that carbon metabolism and oxidative phosphorylation were highly enriched. The increased critical genes of DEGs related to pentose phosphate, glycolysis/gluconeogenesis, and citrate cycle pathways might be essential for soluble sugar degradation. Analysis of DEGs implicated in ascorbate revealed the myoinositol pathway required to accumulate ascorbic acid. Finally, DEGs involved in endogenous phytohormones and transcription factors, including R2R3 MYB, bHLH, and WRKY, were determined. These findings indicated that GA3-trigged morphological alterations might be related to the primary metabolites, hormone signaling, and transcription factors, providing potential candidate genes that could be guided to enhance the fruit development of R. roxburghii in practical approaches.

Natural Nitrogen-Bearing and Phosphorus-Bearing Nanoparticles in Surface Sediments of the Pearl River Estuary, China: Implications for Nitrogen and Phosphorus Cycling in Estuarine and Coastal Ecosystems

Eutrophication creates multiple environmental problems, threatening the ecological security and sustainability of estuarine and coastal ecosystems worldwide. Key nutrients of concern are nitrogen (N) and phosphorus (P), which are the main controls in eutrophication. Considering that sediments are inseparable sinks of N and P, concern has grown regarding the forms in which N and P occur in the surface sediments of estuaries and coastal areas. Nonetheless, studies on the natural N-bearing or P-bearing nanoparticles in estuarine and coastal sediments have rarely been reported. Herein, the surface sediments (0–5 cm) of the Pearl River Estuary in China were collected and subjected to analysis. Using high-resolution transmission electron microscopy (HR-TEM) analysis, numerous natural N-bearing and P-bearing nanoparticles were observed. The results revealed that there are some differences in the occurrence forms of N and P in nanoparticles, suggesting that N and P could be adsorbed by nanoparticles of minerals such as hematite, goethite, muscovite, anorthite and quartz in estuarine and coastal environments, and further form N-bearing and P-bearing nanoparticles. These nanoparticles contained small amounts of N (1.52–3.73 wt%) and P (0.22–1.12 wt%), and were mainly single crystal or polycrystalline in form, with sizes ranging from 10 nm × 50 nm to 250 nm × 400 nm. In addition, P was shown to exist in the form of Ca and Fe phosphate nanoparticles in the estuarine sediments. The Ca and Fe phosphate nanoparticles had higher phosphorus content (5.02–9.97 wt%), mainly amorphous, with sizes ranging from 50 nm × 120 nm to 250 nm × 400 nm. Moreover, N-bearing and P-bearing nanoparticles could influence the migration, precipitation and release processes of N and P, and play a certain role in the N-cycling and P-cycling of estuarine and coastal ecosystems. Furthermore, we explored the role of N-bearing and P-bearing nanoparticles in the N-cycling and P-cycling in estuarine and coastal ecosystems. Thus, this study could provide new ideas for water environment management and other related research fields.

Seasonal variation regulate the endogenous phosphorus release in sediments of Shijiuhu Lake via water-level fluctuation

Freshwater lakes undergo substantial alterations of the phosphorus (P) cycle in the water-sediment ecosystem due to thermal change. The impact process of seasonal fluctuation on P cycling in sediments has been scarcely investigated. P forms in sediments from a freshwater lake in China were analyzed using sequential extraction technique. The vertical distribution of soluble reactive P (SRP), Fe2+, and S2− in the interstitial water was measured using diffusion gradient technique (DGT). Fick's Law and DIFS model were used to obtain the diffusion fluxes of SRP and the kinetic parameters in the water-sediment system. The results showed that total P (TP) concentrations in the solid sediments varied from 207.5, 266.6 and 130.3 mg/kg to 614.7, 1053.1, and 687.6 mg/kg in winter, spring, and summer, respectively. The concentrations of individual P forms in spring were higher than those in other seasons, with Fe-bound P (Fe–P) concentration being the highest across all seasons. Notably, significant variations of SRP concentrations were found in the interstitial water between sedimentary depths of approximately 2 cm and 6 cm, particularly in the summer. Furthermore, higher diffusion fluxes of SRP through the interface were found in summer. A stable anaerobic environment failed to develop in spring with high water level, preventing the desorption of solid Fe–P and diffusion of Fe2+ into the water due to the afflux and deposition of P-containing particulate into deeper sediment layers along with organic material. Under extreme high-temperature in summer, decreased rainfall and rising temperatures boosted the activity of aquatic organisms in the water, thereby reducing P fixation by sediments and leading to P release. This process increased the risk of P excess and potential eutrophication in the water. Generally, clarifying the resupplying processes of endogenous P in sediment systems experiencing seasonal variations is critical for eutrophication management of lakes.

Plastic residues alter soil microbial community compositions and metabolite profiles under realistic conditions

Wide usage of plastic coupled with mismanagement has created a humongous environmental hazard threatening entire ecosystems. To date, the potential effects of plastic debris-induced soil nutrition substance changes and the relevant microbial metabolic behavior remain unclear. Here, we studied the effect of plastic films polyethylene and polylactic acid in differential soil environments (farmland, woodland, and wetland) for 120 days. Soil enzyme activities (urease, neutral phosphatase, and catalase) and nutrition substance (NH4+-N, available P, available K, and soil organic matter) present obvious variations in polylactic acid groups compared to polyethylene-treated samples. 16S rRNA gene sequencing indicates that several bacteria abundance such as Bacteroidales, Actinobacteriota, Nitrososphaeraceae, Pyrinomonadalcs, Muribaculaceae, exhibited obvious up-regulation or down-regulation, and simultaneously, the carbon, nitrogen, and phosphorus cycling relevant species Bryobacter, Bradyrhizobium, and Sphingomonas, expressed wider margin of down-regulation in abundance in plastic treatment soil samples. As a result, the abundance of metabolites including sugar, amino acid, and fatty acids, which may associated with nutrition substance metabolic pathways, were significantly altered in the stress of plastic. These findings provide valuable information on the environmental effects of plastics, and the relationships of subsequent nutrition substance changes and microbial metabolic behavior.

Deposition is a phosphorus source for Fallopia japonica during early-stage primary succession

Phosphorus is a key plant nutrient linked to plant growth during the early stages of primary succession in volcanic soils. Available phosphorus is thought to include soil and atmospheric phosphorus, but it is not well understood. Here, we focused on deposition as a potential phosphorus source. We evaluated the contribution of deposition to phosphorus uptake and growth in Fallopia japonica, a key pioneer species of primary succession. When we experimented with growing F. japonica under field conditions, F. japonica not covered by a roof absorbed more phosphorus than that covered by the roof, suggesting the influence of total (dry + wet) deposition. Furthermore, we tested the effects of deposition by treating F. japonica seedlings with wet deposition or distilled water in six volcanic soils. Plants that received the wet deposition treatment exhibited higher phosphorus contents and growth rates than those treated with distilled water. The phosphorus from wet deposition and the phosphorus from soil contributed nearly equally to F. japonica development. Our findings suggest that F. japonica grows during primary succession and builds up the phosphorus cycle by absorbing a trace amount of phosphorus from deposition and volcanic soils.

Recognizing the variation of DNA-P during and after the algal bloom in lake Hulun

Eutrophication has spread from shallow lakes in temperature zones to lakes in cold regions as a result of a continuous warm climate and human activities. Little proof for the importance of dissolved organic phosphorus (DOP) in contributing to phosphorus cycling and algae growth has been generated for aquatic ecosystems, particularly in cold eutrophic lakes. In this study, a comprehensive in situ study was conducted in overlying water, suspended particulate matter, and sediment during and after algal bloom (in July and September, respectively) in Lake Hulun. Multiple methods of 31P NMR, enzymatic hydrolysis, and UV–visible technologies were combined to detect phosphorus occurrence, bioavailability, and molecular structure from a novel angle. The 31P NMR analysis results showed that DNA-P is mainly stored in the dissolved phase and has not been detected in suspended particulate matter or sediment. Enzymatic hydrolysis was used to determine the bioavailability of DOP, which revealed that in July and September, respectively, 85% and 79% of DOP were hydrolyzable. UV–visible analysis represented that the degree of humification and molecular weight of DOP were high during the algal bloom, but these values considerably dropped following the algal bloom. The large amount of DNA-P present in the overlying water is the main reason for the high degree of humification and high molecular weight of the water body. Besides, Lake Hulun's DNA-P remains highly bioavailable during algal blooms, despite its high degree of humification and molecular weight. These findings can serve as a theoretical basis for understanding the migration and transformation of DOP, as well as the persistence of algal blooms in eutrophic lakes located in cold regions.

Manipulating rhizosphere microorganisms to improve crop yield in saline-alkali soil: a study on soybean growth and development.

Rhizosphere microorganisms can effectively promote the stress resistance of plants, and some beneficial rhizosphere microorganisms can significantly promote the growth of crops under salt stress, which has the potential to develop special microbial fertilizers for increasing the yield of saline-alkali land and provides a low-cost and environmentally friendly new strategy for improving the crop yield of saline-alkali cultivated land by using agricultural microbial technology. In May 2022, a field study in a completely randomized block design was conducted at the Heilongjiang Academy of Agricultural Sciences to explore the correlation between plant rhizosphere microorganisms and soybean growth in saline-alkali soil. Two soybean cultivars (Hening 531, a salt-tolerant variety, and 20_1846, a salt-sensitive variety) were planted at two experimental sites [Daqing (normal condition) and Harbin (saline-alkali conditions)], aiming to investigate the performance of soybean in saline-alkali environments. Soybeans grown in saline-alkali soil showed substantial reductions in key traits: plant height (25%), pod number (26.6%), seed yield (33%), and 100 seed weight (13%). This underscores the unsuitability of this soil type for soybean cultivation. Additionally, microbial analysis revealed 43 depleted and 56 enriched operational taxonomic units (OTUs) in the saline-alkali soil compared to normal soil. Furthermore, an analysis of ion-associated microbes identified 85 mOTUs with significant correlations with various ions. A co-occurrence network analysis revealed strong relationships between specific mOTUs and ions, such as Proteobacteria with multiple ions. In addition, the study investigated the differences in rhizosphere species between salt-tolerant and salt-sensitive soybean varieties under saline-alkali soil conditions. Redundancy analysis (RDA) indicated that mOTUs in saline-alkali soil were associated with pH and ions, while mOTUs in normal soil were correlated with Ca2+ and K+. Comparative analyses identified significant differences in mOTUs between salt-tolerant and salt-sensitive varieties under both saline-alkali and normal soil conditions. Planctomycetes, Proteobacteria, and Actinobacteria were dominant in the bacterial community of saline-alkali soil, with significant enrichment compared to normal soil. The study explored the functioning of the soybean rhizosphere key microbiome by comparing metagenomic data to four databases related to the carbon, nitrogen, phosphorus, and sulfur cycles. A total of 141 KOs (KEGG orthologues) were identified, with 66 KOs related to the carbon cycle, 16 KOs related to the nitrogen cycle, 48 KOs associated with the phosphorus cycle, and 11 KOs linked to the sulfur cycle. Significant correlations were found between specific mOTUs, functional genes, and phenotypic traits, including per mu yield (PMY), grain weight, and effective pod number per plant. Overall, this study provides comprehensive insights into the structure, function, and salt-related species of soil microorganisms in saline-alkali soil and their associations with salt tolerance and soybean phenotype. The identification of key microbial species and functional categories offers valuable information for understanding the mechanisms underlying plant-microbe interactions in challenging soil conditions.

Soil Microbial Community and Soil Abiotic Factors Are Linked to Microorganisms’ C:N:P Stoichiometry in Larix Plantations

Ecological stoichiometry is an essential tool to understand carbon (C), nitrogen (N), and phosphorus (P) cycles and nutrient limitations. Plantations are usually managed to maintain specific age structures, but the impact of such management on microbial biomass and stoichiometric ratios remains unclear. We compared the stand ages of four Larix principis-rupprechtti Mayr. Plantations that were 15 years old, (young plantation, Lar15), 24 years old, (middle aged plantation, Lar24), 40 years old, (near-mature plantation, Lar40), and 50 years old, (mature plantation, Lar50), respectively, to determine the main factors that drive differences in the C:N:P stoichiometry of microorganisms. We demonstrated that the temperature, moisture, and nutrient concentrations in surface soil increased significantly with forest age. The stoichiometric ratios of elements in soil and microorganisms reached their maxima in the Lar40 and Lar50 plantations. Additionally, forest stand ages had a great influence on the biomass of microbial communities. Moreover, soil microbial community and soil abiotic factors are closely related to soil microorganisms’ C:N:P stoichiometric ratios. Specifically, changes in the microbial biomass C:N (MBC:MBN) were primarily correlated with bacteria, Gram-positive bacteria (G+), temperature, NH4+-N, and moisture in soil. Shifts in G+, actinobacteria, soil temperature, and total phosphorus were primarily associated with variation in microbial biomass C:P (MBC:MBP). Alterations in microbial biomass N:P (MBN:MBP) were correlated with bacteria, NH4+-N, water content, Gram-negative bacteria, and soil temperature. Overall, these results suggest that microbial elemental stoichiometric ratios could be affected by stand age and emphasize the importance of microbial communities and soil abiotic factors in shifting this dynamic change process.