Evaluation of the impact of aquatic-animal excretion on nutrient recycling and retention in stormwater treatment wetlands

Abstract

The relative contribution of aquatic animals to phosphorus (P) and nitrogen (N) cycles of water bodies can be substantial. Excretion rates can be affected by seasonal environmental conditions, physiology, and body size. Determining the importance of particular species or size classes to total fauna-driven internal recycling of nutrients requires measurements of seasonal mass-specific excretion rates (MSER). We used short-term incubation experiments to estimate the contribution of excretion and egestion by eight species of fish and one macroinvertebrate to water column P and N in Stormwater Treatment Areas (STAs) used to retain P from water flowing to the Everglades in Florida, U.S.A. To account for environmental conditions and fish physiology, excretion was estimated during day and night in the wet and dry seasons for nine of the most abundant fish and invertebrates found in the STAs. To yield measurable contributions of analytes in short-duration incubations, large-fish species were incubated individually, whereas small-fish species and invertebrates were incubated in single-species groups. For large-fish species, total excreted nutrients were positively correlated with the mass of individual fish, but MSER decreased with increased individual size. All four small species, three fish and invertebrate, excreted P and N at greater MSERs than all five large-fish species. Most MSERs were not correlated with incubated mass (stocked density) of small animals (80%), indicating that there were no density-dependent effects on excretion rates. Most species excreted more N and less P during the South Florida wet season (May to October) though this was not the case for one or more analytes for three of the large species. Most species excreted more during the day, except large herbivores that excreted more at night. Small species excreted higher proportions of particulate P and N, while the larger species had higher proportions of dissolved nutrients. Using measured MSERs and areal biomass estimates for the nine species, we estimated mass of P and N excreted annually. Based on known inflow P and N loads for four STAs, a preliminary and conservative estimate is that fish excretion can recycle between 50% to 92% of the annual total P entering the system and 15% to 29% of the annual total N entering the system. These preliminary results underestimate the actual amounts because they do not account for STA cells that were not sampled, nor known under-estimation of large-fish biomass, as well as other animal effects on nutrient cycles such as bioturbation. Though preliminary, our results suggest that altering aquatic animal abundance or species composition could improve the efficiency of STAs to remove and retain nutrients from inflowing waters.