Mechanisms of soil respiration and its temperature sensitivity in black soil farmland

Abstract

Soil respiration is the process of decomposition of organic matter in soil and microbial metabolism to produce carbon dioxide. In the context of climate warming and degradation of agricultural soil fertility, it becomes crucial to explore the mechanisms of soil respiration to increase carbon sequestration. In this paper, five biochar applications (0, 3.79, 7.58, 11.36, 15.15 t/ha) and two tillage methods (shallow ploughing, deep ploughing) were set up to explore the mechanism of soil respiration and its temperature sensitivity by studying the effects of tillage methods and different amounts of biochar application on soil properties, soil enzyme activities, soil respiration and its temperature sensitivity during the growing season of soybeans in the black soil area. The results showed that soil respiration rate increased with biochar application in the low biochar application range; the opposite trend was observed for high biochar application beyond the threshold (11.36 t/ha). The application of 11.36 t/ha of biochar amount had the strongest promotion effect on soil respiration, reaching 2.42 μmol m−2·s−1 in the shallow ploughing treatment and increasing 0.32 μmol m−2·s−1 in the deep ploughing compared to the shallow ploughing. The application of biochar significantly increased the activity of nitrogen and phosphorus enzymes and had an inhibitory effect on carbon cycle enzymes; the increased activity of nitrogen and phosphorus cycle enzymes provided sufficient substrate for microbial respiration and thus increased soil respiration, and the decrease in the enzymatic reaction of carbon cycle enzymes led to a decrease in temperature sensitivity. The temperature sensitivity of deep ploughing (2.18–2.59) got a more substantial reduction than that of shallow ploughing (2.34–3.53), which indicates that deep ploughing treatments can better reduce the mineralization of organic carbon than shallow tillage. Meanwhile, the temperature sensitivity of soil respiration reached a minimum with the application of 7.58 t/ha of biochar, which was reduced by 16% under deep ploughing treatment compared to the control, 33% under shallow ploughing treatment compared to the control, and also reduced by 27.27% under deep ploughing compared to shallow ploughing, which suggests that the addition of biochar reduces the loss of carbon to a certain extent. This study is important for assessing the effects of tillage practices and biochar combinations on carbon sequestration in agricultural soils in black soil areas.