Orange Essential Oil Research Articles

Adaptogenic effects of essential oils: prognosis in vitro and results in vivo

The results of a two-week in vivo experiment confirmed the adequacy of the in vitro prognosis about the possibility of oxidative balance correction. The prooxidant effects of cedar, orange and lavender essential oils (by inhalations) on the free radical production by blood phagocytes were investigated using a chemiluminescent (CL) test. The results obtained in vivo are in good agreement with those obtained in vitro models. Consequently, with the help of express CL analysis, it is possible to prognose the direction and intensity of changes in the free radical balance of the internal environment of the body under the influence of essential oils. Two conditions are necessary for such a forecast. The first, preliminary definition of the functional status of the blood phagocytes using express CL-analysis is required for an individual person. Secondly, information is required about the antioxidant or prooxidant potential of essential oils, which the person has chosen when he oriented on organoleptic parameters or objective recommendations. Individual selection of essential oils as biologically active metabolic regulators will avoid the risk of unwanted side effects in aromatherapy. Using this approach, manufacturers and distributors and consumers receive a preliminary assessment of the antioxidant and prooxidant activity of essential oils.

The effect of addition sweet orange essential oil and penicillin in tris yolk extender to Simmental liquid semen against percentage motility, viability and abnormalities of spermatozoa

One of the causes a poor quality of liquid semen is increasing bacterial growth. The addition of sweet orange essential oil and penicillin in Simmental liquid semen is expected to improve the quality of liquid semen because it contains antibacterial. The purpose of this research is to know the value of the percentage of motility, viability and spermatozoa abnormalities in Simmental liquid semen with an addition of combination penicillin with sweet orange essential oil on tris yolk extender. The research hypothesis is the addition of combination penicillin with sweet orange essential oil to tris yolk extender can increase the value of the percentage of motility, viability and spermatozoa abnormalities in Simmental liquid semen. Materials used in this study is the Simmental fresh semen, tris yolk extender, penicillin, and sweet orange essential oil. The experimental design used randomized design with 5 treatments and 5 replications. Treatment on the research is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameters observed were the evaluation of motility, viability and abnormality spermatozoa before equilibration and after equilibration. The results showed the best result was the addition of 1% sweet orange essential oil and the lowest on the addition of 0% sweet orange essential oil before and after equilibration.

Alkanediol-based deep eutectic solvents for isolation of terpenoids from citrus essential oil: Experimental evaluation and COSMO-RS studies

Current liquid-liquid extraction techniques for fractionation of essential oils rely on hazardous volatile organic compounds to concentrate target solutes for further food, fine chemical and medical applications. Herein, bio-renewable deep eutectic solvents based on various alkanediols were assessed as environmentally friendly alternatives to separate bioactive terpenoids from terpenes for essential oil downstream processing. In particular, DES composed of choline chloride as hydrogen bond acceptor and alkanediols including 1,3-butanediol, 2,3-butanediol, 1,2-propanediol or 1,3-propanediol as hydrogen bond donors at 1:2 ratio were used for fractionation of citrus essential oil model mixtures and real crude orange essential oil at mild conditions. The effect of the structure of alkanediols (i.e. length of hydrocarbon chain and the position of hydroxyl group) on the essential oil extraction performance provided by the different DES was explored in terms of experimental liquid-liquid equilibrium, distribution coefficients and selectivities. Additionally, the experimentally measured thermodynamic parameters were compared against those estimated using COnductor-like Screening MOdel for Real Solvents as a tool for solvent selection. Lastly, a further thermodynamic understanding of the behavior of the systems was performed by analysis of the computed sigma profiles, activity coefficients, excess enthalpies and molecular interactions between the terpenic hydrocarbon solutes and the solvents. Overall, the proposed alkanediol-based DES are feasible solvents for essential oil fractionation, arising as promising candidates to develop sustainable essential oil purification processes through selective isolation of terpenoid compounds.

The Role of Gelatin-Based Film Coating Combined with Orange Peel Essential Oil on the Quality of Refrigerated Shrimp

In this study, application of gelatin-based film coating combined with essential oil obtained from orange (Citrus sinensis (L.) Osbeck) peel on the quality of deep water pink shrimp (Parapenaeus longirostris Lucas 1846) was aimed. Gelatin film (GF) and gelatin film incorporated with orange peel essential oil (GF+EO) were used for film coating. Thickness and microstructure of the films, nutritional composition (protein, moisture, lipid, ash and free amino acid content), sensory and melanosis evaluation, chemical [Sulphur dioxide (SO2), pH, total volatile base nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), thiobarbituric acid (TBA), peroxide value (PV), free fatty acid (FFA)], physical (color) and microbiological [total viable counts (TVC), total psychotropic bacteria (PB), total coliform bacteria (TCB) and Enterobacteriacea (EB) ] were carried out for 15 days. As a result, gelatin film coatings were effective on the quality and shelf life of shrimps than the control group. Gelatin films were more effective on melanosis than control and the essential oil protected the shrimp’s quality more than the other groups. According to the sensorial and microbiological analysis results, control group samples (C) have 7 days’ shelf life while gelatin group samples (GF) have 12 days, and gelatin film with orange peel essential oil coated group samples (GF+EO) have 15 days.

Antibacterial activity, optical, mechanical, and barrier properties of corn starch films containing orange essential oil

The incorporation of antimicrobial compounds into natural polymers can promote increased shelf life and ensure food safety. The aim of this study was to evaluate the antibacterial activity, morphological, optical, mechanical, and barrier properties of corn starch films containing orange (Citrus sinensis var. Valencia) essential oil (OEO). The corn starch films were prepared using the casting method. OEO and the corn starch films incorporated with OEO showed higher antibacterial activity against Staphylococcus aureus and Listeria monocytogenes. The addition of OEO to the films increased the morphological heterogeneity and contributed to the reduction of the tensile strength and elongation of the films, and it increased the moisture content, water solubility, and water vapor permeability. The water vapor permeability and partial or total solubility of a film in water prior to consumption of a product are of interest when the film is used as food coating or for encapsulation of specific molecules.

Essential Oils-Loaded Polymer Particles: Preparation, Characterization and Antimicrobial Property.

In the last few years, essential oils (EOs) derived from plants have aroused great interest due to their well-known antimicrobial activity. Unfortunately, they present several limitations in their use, such as photosensitivity, temperature sensitivity, high volatility, and poor water solubility. The encapsulation technique represents a good solution to these problems and ensures protection of the functional properties of essential oils. In this work, bergamot essential oil (BEO) and sweet orange essential oil (OEO) loaded-Eudragit® RS 100 (EuRS100) nanoparticles (NPs) were prepared by using the nanoprecipitation technique. We obtained nanoparticles characterized by a mean diameter of 57 to 208 nm and a positive surface charge (39 to 74 mV). The antibacterial activity of the obtained systems against Escherichia coli was in vitro investigated. We demonstrated that both orange and bergamot essential oils were successfully encapsulated and our nanoparticles have good antibacterial activity. Finally, in order to evaluate the potential applicability of OEONps to prolong fresh orange juice shelf-life, survival of E. coli during a storage period of one week at 25 °C was investigated: Orange essential oil-loaded nanoparticles (OEONPs) have been able to prolong the orange juice shelf life.

Comparing effects of aromatherapy with lavender essential oil and orange essential oil on fatigue of hemodialysis patients: A randomized trial

Background and purposeFatigue is a common complication of hemodialysis. This study aimed to compare the effects of aromatherapy with essential oils of lavender and orange on the fatigue of hemodialysis patients. Materials and methodsNinety subjects were randomly allocated into three groups of aromatherapy with lavender essential oil, aromatherapy with orange essential oil and control. Data collection tool was the Fatigue Severity Scale. In each experimental group, subjects inhaled five drops of lavender essential oil and orange essential oil. ResultsThe difference in the mean of fatigue before and after the intervention in each of the experimental groups was statistically significant, but this difference was not significant in the control group. Between the two groups of aromatherapy, no significant difference was observed in terms of the mean fatigue after the intervention. ConclusionAromatherapy with lavender essential oil and orange essential oil might reduce fatigue in hemodialysis patients.

L-tryptophan affects the essential oil of navel orange under various growing regions

Abstract Essential oil (EO) of navel orange had anticancer and antimicrobial activities. l -tryptophan and growing regions play important roles in physiological processes of navel orange EO. The application of l -tryptophan under different growing regions is one way of scientific research that has the potential to increase the EO production, so, the aim of this trial was to evaluate the EO isolated from young shoots and peels of navel orange trees under l -tryptophan treatments in West and Upper Egypt. The experiments were conducted at the two Citrus farms located in West and Upper Egypt. Selected trees were subjected to l -tryptophan doses at 0, 200 and 400 mg/L in both regions. The EO of young shoots and peels was isolated by hydro distillation method (HD), then, analyzed by GC and GC/MS equipments. Data were statistically analyzed using ANOVA-2. The EO percentages and constituents of young shoots or peels were significantly affected due to l -tryptophan doses in both regions. The major component of young shoot EO was sabinene while it was limonene of peel EO. The treatment of 400 mg/L ( l -tryptophan) resulted in the greatest amounts of EO (%), sabinene and limonene in west and Upper Egypt. The application of l -tryptophan caused different variations in all chemical classes (MCH, MCHO, SCH and SCHO) of young shoots and peels EO in both regions. l -tryptophan levels produced positive variations on essential oil composition of navel orange trees grown in West and Upper Egypt.

Benzoic Acid Induced Variations in Essential Oil of Valencia Orange Trees Grown in Reclaimed Land of Egypt

Benzoic acid (BA) plays an important role in physiological processes of Valencia orange essential oil. The essential oil of Valencia orange has different medicinal and biological properties. Influences of benzoic acid on essential oil isolated from leaves and peels of these trees were investigated in a new reclaimed region of Egypt. The trees treated with BA at 20 and 40 mg/L compared with an untreated control. Benzoic acid at 20 mg/L resulted in the greatest amounts (0.3 and 0.7%) of essential oil extracted from leaves and peels. The components of sabinene (35.6-37.9%), Δ-3-carene (13.2-14.5%) and limonene (11.1-12.6%) were detected as the major constituents of leaf essential oil; while limonene (70.3-71.5%), myrcene (5.2-7.1%) and γ-terpinene (4.2-5.2%) were detected as main components of peels essential oil. The dose of 20 mg/L BA produced the highest values of all major components. The detected components in essential oil produced from leaves and peels belonged to 4 chemical groups i.e. monoterpene hydrocarbons (MCH), oxygenated monoterpenes (MCHO), sesquiterpene hydrocarbons (SCH) and oxygenated sesquiterpenes (SCHO). The greatest amounts of MCH (79%), SCH (7.5%) and SCHO (1.7%) were recorded with the BA at 20 mg/L, while the highest value of MCHO (18.4%) was recorded with the treatment of 40 mg/L. The results of chemical groups of peels essential oil indicated that control treatments resulted in the highest values of MCHO (10.6%), SCH (3.7%) and SCHO (0.8%) but the greatest value of MCH (88.7%) was recorded with BA at 20 mg/L.

Weight loss effect of sweet orange essential oil microcapsules on obese SD rats induced by high-fat diet.

Obesity is one of the most common and major health concerns worldwide. Weight management through dietary supplements with natural plant extracts has become the focus of current research. Sweet orange essential oil (SOEO) is a natural plant extract, with many bioactivities. In order to evaluate the weight loss effect of SOEO microcapsules and investigate the underlying mechanism, we fed high-fat diet-induced obese SD rats with SOEO microcapsules for 15days and found that SOEO microcapsules reduced body weight gain by 41.4%, decreased total cholesterol level, alleviated liver and adipose tissue pathological alteration. The results of fluorescence quantitative PCR revealed that decreasing the expression of peroxisome proliferators-activated receptor-γ, upregulating of uncoupling protein 2, hormone sensitive lipase and carnitine palmitoyltransferase I, inhibiting the expression of acetyl-CoA carboxylase appear to be the mechanism of SOEO microcapsules to lose weight. This study suggests that SOEO microcapsule is a potential dietary supplement for weight loss. Abbreviations: SOEO: sweet orange essential oil; TC: total cholesterol; TG: triglyceride; LDL-c: low-density lipoprotein cholesterol; HDL-c: high-density lipoprotein cholesterol; PPARα: peroxisome proliferators-activated receptor-α; PPARγ: peroxisome proliferators-activated receptor-γ; UCP2: uncoupling protein 2; HSL: hormone sensitive lipase; CPT1: carnitine palmitoyltransferase I; ACC: acetyl-CoA carboxylase; NPY: neuropeptide Y; LEP: leptin; INS: insulin; ALT: alanine aminotransferase; AST: aspartate aminotransferase.

Utilization Of Frozen Goat Semen With Addition Sweet Orange Essential Oil To Improve Genetic Quality in Ujung Teran Village

One of the causes of underdeveloped goats is low genetic quality. The way that can be done to improve the genetic quality of goats is to do cross-breeding with superior goats through Artificial Insemination. The community service aims to increase the knowledge of goat farmers in Ujung Teran Village, Langkat Regency on how to make goat frozen semen using tris yolk extender plus sweet orange essential oil for Artificial Insemination program along with the benefits that can be obtained. The method of service is by giving lectures and training to goat farmers. The results obtained from the implementation of this program are that farmers know how to make frozen semen of goat using sweet orange essential oil on the tris yolk extender and are willing to make Artificial Insemination in their goat cattle.

Chemical Composition and Antibacterial Activity of the Essential Oil of Citrus aurantium L. Growing in Eastern Algeria

Citrus aurantium (Bitter Orange) is a Rutaceae known for its extremely bitter and sour taste. Its leaves are rich in essential oil (EO). The purpose of this study was to extract, analyse and evaluate the antibacterial activity of this EO in vitro, against 10 bacterial strains responsible for nosocomial infections (05 Escherichia coli, 03 Staphylococcus aureus and 02 Klebsiella ssp). The extraction of the essential oil was carried out on fresh leaves harvested in Annaba (Eastern Algeria) using a Clevenger type device. The analysis was performed by GC/MS and it was tested on 10 bacterial strains by the dilution method in agar medium. The results showed that the EO is composed mainly of linalool (44.52%). Among the ten strains tested, eight were sensitive to this EO with inhibition diameters ranging from 12.1 mm to 21.45 mm and minimum inhibitory concentration (MIC) between 0.1% and 1%, however, both Klebsiella pneumoniae and Escherichia coli ATCC strains were resistant. The antibacterial activity of Bitter Orange EO seems to be largely due to the major component linalool.

Effects of orange essential oil on intestinal microflora in mice.

The intestinal microbiota has a wide variety of functions in the host. A positive effect of plant extract on intestinal microbiota in animals has been reported. However, the effect of orange essential oil and its components limonene, linalool and citral on intestinal microflora in mice has seldom been reported. The effects of intragastric administration of orange essential oil and limonene, linalool and citral on intestinal microflora and biochemical indexes in mice were studied. The effect of essential oil, linalool and citral on immune organ index (spleen and thymus index), IgM and IL-2 was not significant (P>0.05). A significant increase (P<0.05) of H+ K+ -ATPase activity, IgA, IgG, and IL-2 in the limonene group was observed when compared with the control group. Orange essential oil, limonene, linalool and citral could significantly reduce the content of short-chain fatty acids in the cecum and colon of mice. Principal coordinates analysis showed that intestinal bacterial structure of limonene group cecum and colon was apparently separated from other groups. The relative abundance of Lactobacillus in cecum and colon in essential oil, limonene, linalool and citral groups was higher than that in the control group. Orange essential oil and limonene, linalool and citral could affect the intestinal microflora of mice, and enhance the relative abundance of Lactobacillus. The intestinal bacterial structure of cecum and colon in the limonene group was quite different from other groups. This indicated a more obvious effect of limonene on intestinal bacteria, also resulting in significant changes in blood immune index and short-chain fatty acids in mice. © 2019 Society of Chemical Industry.

Implementing quiet hours on a medical telemetry unit

Implementing quiet hours on a medical telemetry unit

Extraction of 'Gannanzao' Orange Peel Essential Oil by Response Surface Methodology and its Effect on Cancer Cell Proliferation and Migration.

The essential oil of ‘Gannanzao’ orange peel was extracted by hydrodistillation, and the extraction conditions were optimized by Box–Behnken response surface methodology. The components of essential oil were analyzed by GC-MS. Thirty-nine different components were detected, accounting for 99.59% of the total oil. Limonene (88.07%) was the prominent component. The optimal extraction conditions were as follows: liquid material ratio of 8.4:1 (mL/g), sodium chloride concentration of 5.3%, and distillation time of 3.5 h. The Cell Counting Kit-8 assay showed that ‘Gannanzao’ orange peel essential oil had good dose-dependent inhibition effect on the proliferation of HepG2 hepatoma cells and HCT116 colorectal cancer cells. When the concentration of the essential oil was 0.6 μL/mL or higher, the viability rate of both cancer cells became lower than 13.0%. The transwell assay indicated the essential oil can inhibit migration of both cancer cells at the concentration of 0.3 μL/mL.

Effect of lemon and orange peel essential oils on performance of broiler chickens during summer

The present experiment was conducted to study the effect of lemon and orange peel essential oils on performance of broilers during summer season. The present research work was conducted on 240 Vencobb 400 straight-run broiler chickens. The day old chicks were randomly distributed into 4 treatment groups with 3 replicates of 20 chicks each. The control group received basal diet and the different treatment groups received basal diet supplemented with lemon peel essential oil at 200 mg/kg, orange peel essential oil at 200 mg/kg and combination of lemon and orange essential oil at 200 mg/kg feed. At the end of the study, the broilers supplemented with lemon or orange peel essential oil either alone or in combination recorded significantly higher (P<0.01) body weight and gain in weight than control. The percent improvement in FCR at sixth week in broilers fed lemon peel essential oil, orange peel essential oil and combination of these essential oils over the control was 8.62, 3.44 and 10.91%, respectively. The E. coli count was significantly (P<0.01) reduced in treatment group offered blend of lemon and orange peel essential oils. The supplementation of lemon and orange peel essential oil either singly or in combination in diets during summer season was found to be beneficial to enhance growth performance, gut health and margin of profit in broiler production.

Natural green-peel orange essential oil enhanced the deposition, absorption and permeation of prochloraz in cucumber.

Pesticides have long been used in agricultural production and will continue to be used long into the future. Enhancing their efficient use in agricultural production is an effective method to avoid their loss to non-target areas, such as soil, water and air. Here natural green-peel orange essential oil (GOEO) was used as a spray adjuvant to improve the efficient use of pesticides. A comparative study between a conventional formulation and a conventional formulation with GOEO was conducted. Conventional formulations of prochloraz were chosen as control groups. It was obvious that the concentrations of prochloraz and its metabolite in cucumber leaves treated by conventional formulations with GOEO were much higher than those treated without GOEO. The data also showed, for both emulsifiable concentrate (EC) and suspension concentrate (SC), that the spreading and penetration performance were improved when GOEO was used as a spray adjuvant, but with SC the impact was more significant. GOEO improved the deposition, absorption and degradation performance of prochloraz on cucumber leaves. It was more applicable to those pesticide formulations without a good wetting and spreading effect, such as SC. As a plant source extract, GOEO is much safer for crops and more friendly to the environment than other synthetic adjuvants. The application of GOEO as a spray adjuvant has great potential to increase the pesticide utilization rate.

In vitro and in vivo studies of novel fabricated bioactive dressings based on collagen and zinc oxide 3D scaffolds

The paper reports the synthesis and physico-chemical and biological characterization of novel wound dressings based on collagen and essential oil functionalized ZnO nanoparticles intended to improve the treatment of burns and to reduce the risk for developing wound sepsis in patients with burns or chronic wounds. The prepared wound dressings were physico-chemical characterized by Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). In vitro biocompatibility and cytotoxicity was proved on human fibroblast cells, antimicrobial potential was assessed on Gram positive and Gram negative bacteria models (Staphylococcus aureus and Escherichia coli, respectively), while in vivo studies were performed on a rat burn wound experimental model. Functionalized ZnO nanoparticles (NPs) proved to range 15–20 nm in size and contain about 1% orange essential oil (EO), which was utilized as a natural antimicrobial agent. NPs are grain-shapped and have a low tendency to form aggregates. No toxicity was noticed in vitro, as human fibroblasts maintained a normal growth and their membrane integrity in the presence of EO functionalized NPs. The capacity of the prepared wound dressings to act as implantable bioresorbable scaffolds that accelerates wounds healing along with an excellent biocompatibility, lack of cytotoxicity and a good antibacterial activity, make these materials promising and safe candidates for wound dressing, especially in burn patients.

PENERAPAN TEKNOLOGI EKSTRAKSI FASA CAIR-GAS PADA PENGEMBANGAN PRODUK BERBASIS JERUK KALAMANSI

Community Service Activity ( CSA ) of the application of science and technology entitled Application of Liquid-Gas Phase Extraction Technology on Development of Kalamansi - Orange - Based has been completed. This activity aims to implement gas-liquid extraction technology, namely steam distillation in extracting essential oils from residues of Kalamansi orange syrup processing, providing knowledge and ways to process Kalamansi orange essential oil into products which have economic value in addition to syrup. It was carried out with the target audience , namely Bhakti Nusa Production Unit in SMKS 16 Bengkulu . It is done to help the production unit to develop Kalamansi-based products by utilizing residues produced by syrup which is rich in essential oils. The products produced on the CSA of the application of science and technology include aromatherapy candles, air freshener gels, anti-mosquito lotions and Bay Tat cakes of Kalamansi jam. The product produced is more widely informed through the socialization activity that received a response and enthusiasm from the audience. Products of CSA of t he application of science and technology are expected to be a superior product from the Bhakti Nusa Production Unit of SMKS 16 Pharmac y as well as to be a top-quality product of the pride of Bengkulu C ity with high economic value. Keywords : Extraction, Liqiud-Gas, Kalamansi, Essential oil

THE ACTIVITY OF ESSENTIAL OILS OBTAINED FROM SPECIES AND INTERSPECIES HYBRIDS OF THE Mentha GENUS AGAINST SELECTED PLANT PATHOGENIC BACTERIA

Plant essential oils of six aromatic herb species and interspecies hybrids of the family Lamiaceae – chocolate mint (Mentha piperita × ‘Chocolate’), pineapple mint (Mentha suaveolens ‘Variegata’), apple mint (Mentha × rotundifolia), spearmint (Mentha spicata), orange mint (Mentha × piperita ‘Granada’) and strawberry mint (Mentha × villosa ‘Strawberry’) – were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas arboricola pv. corylina. The screening was carried out in vitro on agar plates filled with the target organism. All essential oils screened exhibited a higher level of antibacterial activity against A. tumefaciens and X. arboricola pv. corylina than streptomycin used as a standard in all tests. The antimicrobial effect of streptomycin and five mint oils was at the same level for P. syringae pv. syringae. There were no significant differences in the influence of the chocolate mint oil on the growth inhibition of all bacteria tested. Plant essential oils from pineapple mint, apple mint, spearmint and strawberry mint showed the weakest antimicrobial activity against P. syringae pv. syringae and the strongest towards A. tumefaciens and X. arboricola pv. corylina. The essential oils from strawberry mint, pineapple mint, spearmint and apple mint had the strongest effect on A. tumefaciens, and the lowest inhibitory activity was exhibited by the chocolate mint and orange mint essential oils. X. arboricola pv. corylina was the most sensitive to the strawberry mint, pineapple mint and spearmint oils. The chocolate mint oil showed the greatest activity against P. syringae pv. syringae.