The effect of addition sweet orange essential oil and penicillin in tris yolk extender to Simmental liquid semen against percentage motility, viability and abnormalities of spermatozoa

Abstract

One of the causes a poor quality of liquid semen is increasing bacterial growth. The addition of sweet orange essential oil and penicillin in Simmental liquid semen is expected to improve the quality of liquid semen because it contains antibacterial. The purpose of this research is to know the value of the percentage of motility, viability and spermatozoa abnormalities in Simmental liquid semen with an addition of combination penicillin with sweet orange essential oil on tris yolk extender. The research hypothesis is the addition of combination penicillin with sweet orange essential oil to tris yolk extender can increase the value of the percentage of motility, viability and spermatozoa abnormalities in Simmental liquid semen. Materials used in this study is the Simmental fresh semen, tris yolk extender, penicillin, and sweet orange essential oil. The experimental design used randomized design with 5 treatments and 5 replications. Treatment on the research is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameters observed were the evaluation of motility, viability and abnormality spermatozoa before equilibration and after equilibration. The results showed the best result was the addition of 1% sweet orange essential oil and the lowest on the addition of 0% sweet orange essential oil before and after equilibration.