ABSTRACT A technique using fluorescent photography has been developed for a quantitative analysis of incorpora-tion of soil-applied chemicals. Samples of known pig-ment concentrations were used to establish a calibration curve of pigment concentration vs. film optical density. Fluorescent pigments were used as a tool in evaluating uniformity of soil incorporated chemicals. When applied at the rates used in this study, they emit visible light in proportion to their concentration in the soil. Standard samples of known pigment concentration can be mixed and used in a calibration procedure to measure unknown amounts of pigment in the same type soil at the same moisture content. Separate calibrations must be per-formed for different types of soil or for different moisture contents. The field sampling technique used in this study shows that pigment can be mixed easily with water and applied at rates that are reproducible. Analyses of these field samples show that differences in concentration in the soil profile can be detected, and a matrix of concentration can be constructed.