Non-cancerous Adjacent Colorectal Tissues Research Articles

Expression of DNM3 is associated with good outcome in colorectal cancer.

The aim of this study is to reveal the potential value of dynamin3 (DNM3) in colorectal cancer (CRC) evaluation of clinical diagnosis and prognosis. A total of 100 tissue samples were collected from 50 patients with stages I–IV, CRC tissues (n = 50) paired with non-cancerous adjacent colorectal tissues (n = 50). The expression levels of DNM3 were detected in 50 cases of CRC tissues and 50 cases of non-cancerous adjacent colorectal tissues by real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemical method (IHC) was conducted to semi-quantify the expression of DNM3 protein. Results showed that the relative expression of DNM3 mRNA in CRC tissues was 0.634-fold of that in non-cancerous adjacent colorectal tissues. The positive rate of DNM3 protein in CRC tissues (42.0%) was much lower than that in non-cancerous adjacent colorectal tissues (66.0%; P < 0.05). The expression level of DNM3 protein in CRC tissues was dependent on tumor size, degree of histological differentiation, and clinical stage (P < 0.05). The expression level of DNM3 mRNA in CRC tissues was significantly correlated with tumor size and pathology classification (P < 0.05). The research shows that detecting the expression of DNM3 helps in analyzing the tumor size, degree of histological differentiation, and clinical stage. Expression of DNM3 may be associated with good outcome in CRC.

Correlation between the expression of membrane-organizing extension spike protein and thymidine kinase 1 with the biological behaviors of colorectal carcinoma

Objective To investigate the expression of membrane-organizing extension spike protein (Moesin), thymidine kinase 1 (TK1) and the clinicopathological parameters of colorectal carcinoma. Methods The expression levels of Moesin and TK1 in 75 cases of colorectal carcinoma tissues and adjacent colorectal tissues were detected by immunohistochemistry. Results The expression rate of Moesin in colorectal carcinoma tissues was higher than that in non-cancerous adjacent colorectal tissues (68.00% vs. 21.33%, t=33.043, P=0.000). The expression levels of Moesin were correlated to histology grade, TNM stage and lymph node metastasis of colorectal carcinoma (respectively, t=4.733, P= 0.030; t=5.171, P= 0.023; t=8.549, P= 0.004). The expression rate of TK1 in colorectal carcinoma tissues was higher than that in non-cancerous adjacent colorectal tissues (80.00% vs. 13.33%, t=66.964, P=0.000). The expression levels of TK1 were correlated to serosa invasion, TNM stage and lymph node metastasis of colorectal carcinoma (respectively, t=1.781, P= 0.182; t=4.383, P= 0.036; t=7.748, P= 0.005). Conclusion Moesin and TK1 are associated with occurrence and development of colorectal carcinoma. Key words: Colorectal carcinoma; Immunohistochemical method; Membrane-organizing extension spike protein; Thymidine kinase 1

Expression and clinical significance of metastasis suppressor 1 and Fascin in colorectal carcinoma

Objective To study the relationship between metastasis suppressor 1 (MTSS1) and Fascin with clinical and pathological characteristics in colorectal carcinoma. Methods Immunohistochemical method was used to detect the expression of MTSS1 and Fascin in 94 cases of human colorectal carcinoma tissues and non-cancerous adjacent colorectal tissues. Results The expression rate of MTSS1 was 38.30% (36/94) in colorectal carcinoma tissues, and 98.94% (93/94) in non-cancerous adjacent colorectal tissues (P<0.01). The expression rate of Fascin was 62.77% (59/94) in colorectal carcinoma tissues, and 25.53% (24/94) in non-cancerous adjacent colorectal tissues (P<0.01). The expression of MTSS1 was related to serosa invasion, TNM stage and lymph node metastasis (P<0.05), and there was a close relationship between the expression of Fascin and histology grade, TNM stage and lymph node metastasis (P<0.05). Conclusion The low expression of MTSS1 and high expression of Fascin could confer a poor prognosis of patients with colorectal carcinoma. Key words: Metastasis suppressor 1; Fascin; Colorectal carcinoma

Expression of signal transducer and activator of transcription 3 and glioma-associated oncogene 1 and clinical significance in colorectal carcinoma

Objective To explore the expression of signal transducer and activator of transcription 3(STAT3),and glioma-associated oncogene 1(Gli1)and the clinical significance in colorectal carcinoma. Methods Ninety-nine cases of colorectal carcinoma tissues and non-cancerous adjacent colorectal tissues were obtained for detection of the expression of STAT3 and Gli1 by immunohistochemistry.The relationship between the expression of STAT3 and Gli1 in colorectal carcinoma tissues and clinicopathologic features were analyzed. Results The expression rate of STAT3 and Gli1 in colorectal carcinoma tissues was higher than that in non-cancerous adjacent colorectal tissues (60. 87% vs.11. 59%, and 75. 36% vs.37. 68%,P<0. 01).The expression of STAT3 was related to histology grade, serosa invasion, TNM stage and lymph node metastasis of colorectal carcinoma (P<0. 05).There was a close relationship in colorectal carcinoma between the expression of Gli1 and histology grade, TNM stage and lymph node metastasis(P< 0. 05). Conclusion Simultaneous detection of STAT3 and Gli1 might be helpful for evaluating the malignance and predicting the metastatic potential of colorectal carcinoma. Key words: Signal transducer and activator of transcription 3; Glioma-associated oncogene 1; Colorectal carcinoma

Relationship of Golgi protein-73 and CD133 expression with clinical biological behaviors of colorectal carcinoma

Objective To analyze the relationship between the expression of Golgi protein-73 (GP73) and CD133 with clinical biological behaviors of colorectal carcinoma.Methods Immunohisto-chemical method was used to assess the expression of GP73 and CD133 in 82 cases of colorectal carcinoma tissues and non-cancerous adjacent colorectal tissues.Results The expression rate of GP73 in colorectal carcinoma tissues was higher than that in non-cancerous adjacent colorectal tissues (84.15％ vs.64.63％,P ＜0.01).The expression level of GP73 was related to histology grade and serosa invasion of colorectal carcinoma (P ＜ 0.05).Tthe expression rate of CD133 in colorectal carcinoma tissues was higher than that in non-cancerous adjacent colorectal tissues (47.56％ vs.15.85％,P ＜0.01),and the expression of CD133 was closely related with TNM stage and lymph node metastasis of colorectal carcinoma (P ＜0.05).Conclusion GP73 and CD133 in colorectal carcinoma tissues might be used as markers for assessing the clinicopathological features. Key words: Colorectal carcinoma; Golgi protein-73; CD133

Expression of human runt-related transcription factor 3 and signal-induced proliferation-assocciated gene1 in gastric carcinoma and clinical significance

Objective To investigate the expression of RUNX3 and signal-induced proliferationassocciated gene1 (Sipa1) in gastric carcinoma,and their relation to the clinicopathological features.Methods RUNX3 and Sipa1 expression in 62 cases of gastric carcinoma tissues and non-cancerous adjacent colorectal tissues was examined by using immunohistochemical method.Results The expression rate of RUNX3 was 62.90％ (39/62) in gastric carcinoma tissues,and 93.55％ (58/62) in non-cancerous adjacent gastric tissues (P ＜0.01).The expression of RUNX3 in gastric carcinoma was related to histology grade and lymph node metastasis (P ＜0.05).The expression rate of Sipa1 was 43.55％ (27/62) in gastric carcinoma tissues,and 80.65％ (50/62) in non-cancerous adjacent gastric tissues (P ＜ 0.01).There was a close relation between the expression of Sipa1 and histology grade,TNM stage and lymph node metastasis of gastic carcinoma (P ＜ 0.05).Conclusion Expression of RUNX3 and Sipa1 correlates to the biological behaviors of gastric carcinoma. Key words: Gastric carcinoma; Immunohistochemical method; Human runt-related transcription factor 3 ; Signal-induced proliferation-assocciated gene1

Significance and expression of stathmin and endoglin in colorectal carcinoma

Objective To analyze the relationship between the expression of Stathmin and endoglin-marked microvessel density (MVD) and clinicopathological features of colorectal carcinoma.Methods Immunohistochemistry was used to detect the expression levels of Stathmin and MVD marked with endoglin in 46 cases of human colorectal carcinoma tissues and non-cancerous adjacent colorectal tissues.Results The expression rate of Stathmin was 69.57％ (32/46) in colorectal carcinoma tissues,and 34.78％(16/46) in non-cancerous adjacent colorectal tissues (P ＜0.01).The expression of Stathmin was related to histologic grade,serosa invasion,TNM stage and lymph node metastasis in colorectal carcinoma (P ＜0.05).The expression of MVD marked with endoglin was (68.27 ± 5.66) in colorectal carcinoma tissues,and (41.51 ±4.52) in non-cancerous adjacent colorectal tissues (P ＜0.01).The expression of MVD was related to serosa invasion,TNM stage and lymph node metastasis (P ＜ 0.05).Conclusion The combined detection of Stathmin and endoglin may be used to determine the biological behaviors of colorectal carcinoma. Key words: Stathmin; Endoglin; Microvessel density ; Colorectal carcinoma

Expression of EZH2 and Paxillin in colorectal carcinoma and its relationship with clinical significance and prognosis

Objective To study the relationship between of enhancer of zest homolog 2 (EZH2) and Paxillin with clinical and pathological characteristics in colorectal carcinoma.Methods Immunohistochemical method was used to assess the expression of EZH2 and Paxillin in 85 cases of human colorectal carcinoma tissues and non-cancerous adjacent colorectal tissues.Results The expression rate of EZH2 was 74.12％ (63/85) in colorectal carcinoma tissues,and 45.88％ (39/85) in non-cancerous adjacent colorectal tissues (P ＜0.01).The expression levels of EZH2 were related to histological grade,TNM stage and lymph node metastasis (P ＜ 0.05).The expression rate of Paxillin was 56.47％ (48/85) in colorectal carcinoma tissues,and 21.18％ (18/85) in non-cancerous adjacent colorectal tissues (P ＜ 0.01).There was a close relation between the expression of Paxillin and histological grade,serosa invasion,TNM stage and lymph node metastasis (P ＜ 0.05).Conclusion EZH2 and Paxillin may be used as markers for malignance degree and poor prognosis of colorectal carcinoma. Key words: Enhancer of zest homolog 2; Paxillin; Colorectal carcinoma

Significance of AKT2 and deleted in liver cancer-1 expression in colorectal carcinoma

Objective To analyze the relationship between the expression of AKT2 and deleted in liver cancer-1 (DLC-1) with biological behaviors of colorectal carcinoma.Methods Envision immunohistochemical method was used to assess the expression of AKT2 and DLC-1 in 70 cases of human colorectal carcinoma tissues and non-cancerous adjacent colorectal tissues.Results The expression rate of AKT2 was 60.00％ (42/70) in colorectal carcinoma tissues,and 21.43％ (15/70) in non-cancerous adjacent colorectal tissues (P ＜0.01).The expression rate of DLC-1 was 31.43％ (22/70) in colorectal carcinoma tissues,and 84.29％ (59/70) in non-cancerous adjacent colorectal tissues (P ＜0.01).The expression of AKT2 was related to histology grade,serosa invasion,TNM stage and lymph node metastasis (P ＜ 0.05).There was a close relation between the expression of DLC-1 and serosa invasion,TNM stage and lymph node metastasis (P ＜ 0.05).Conclusion Examination of expression of AKT2 and DLC-1 may be of value in evaluating the biological behaviors of colorectal carcinoma. Key words: AKT2; Deleted in liver cancer-1 ; Colorectal carcinoma