Nanomotor System Research Articles

Light-Driven Biomimetic Nanomotors for Enhanced Photothermal Therapy.

Nanotechnology-based strategy has recently drawn extensive attention for the therapy of malignant tumors due to its distinct strengths in cancer diagnosis and treatment. However, the limited intratumoral permeability of nanoparticles is a major hurdle to achieving the desired effect of cancer treatment. Due to their superior cargo towing and reliable penetrating property, micro-/nanomotors (MNMs) are considered as one of the most potential candidates for the coming generation of drug delivery platforms. Here, near-infrared (NIR)-actuated biomimetic nanomotors (4T1-JPGSs-IND) are fabricated successfully andwe demonstrate that 4T1-JPGSs-IND selectively accumulate in homologous tumor regions due to the effective homing ability. Upon laser irradiation, hyperthermia generated by 4T1-JPGSs-IND leads to self-thermophoretic motion and photothermal therapy (PTT)to ablate tumors with a deep depth, thereby improving the photothermal therapeutic effect for cancer management. The developed nanomotor system with multifunctionalities exhibits promising potential in biomedical applications to fight against various diseases.

Controlling CNT-Based Nanorotors via Hydroxyl Groups.

Nanomotor systems have attracted extensive attention due to their applications in nanorobots and nanodevices. The control of their response is crucial but presents a great challenge. In this work, the rotating and braking processes of a carbon nanotube (CNT)-based rotor system have been studied using molecular dynamics simulation. The speed of response can be tuned by controlling the ratio of hydroxyl groups on the edges. The ratio of hydroxyl groups is positively correlated with the speed of response. The mechanism involved is that the strong hydrogen bonds formed between interfaces increase the interface interaction. Incremental increase in the hydroxyl group concentration causes more hydrogen bonds and thus strengthens the interconnection, resulting in the enhancement of the speed of response. The phonon density of states analysis reveals that the vibration of hydroxyl groups plays the key role in energy dissipation. Our results suggest a novel routine to remotely control the nanomotors by modulating the chemical environment, including tuning the hydroxyl groups concentration and pH chemistry.

Fluidity-Guided Assembly of Au@Pt on Liposomes as a Catalase-Powered Nanomotor for Effective Cell Uptake in Cancer Cells and Plant Leaves.

The fluidity of the liposomes is essential to nanoparticle-membrane interactions. We herein report a liposomal nanomotor system by controlling the self-assembly behavior of gold core-platinum shell nanoparticles (Au@Pt) on liposomes. Au@Pt can aggregate immediately on fluid-phase dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes, forming an uneven distribution. By control of the lipid phase and fluidity, either using pure 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) above its phase transition temperature or adding cholesterol as an adjuvant to DPPC lipids, we precisely control the assembly of Au@Pt on liposomes. Au@Pt maintained high catalase-like activity on the liposomal surface, promoting the decomposition of H2O2 and the movement of the liposomal nanomotors. Finally, we demonstrate that liposomal nanomotors are biocompatible and they can speed up the cellular uptake in mammalian HepG2 cancer cells and Nicotiana tabacum (Nb) plant leaves. This liposomal nanomotor system is expected to be further investigated in biomedicine and plant nanotechnology.

Bienzymatic Spiky Janus Nanomotors Powered by Histamine

AbstractAs an important inflammatory mediator, histamine can be produced by many immune cells (mast cells, neutrophils, dendritic cells, platelets, etc.) and modulates a variety of immune events. Developing enzymatic micro/nanomotors from the perspective of immunometabolism is required but still lacking. In this work, we functionalized the histamine‐metabolizing enzyme onto the Janus Au/mSiO2 nanoparticles. Powered by the tandem catalytic decomposition of histamine initiated by a surface‐modified diamine oxidase, this nanomotor system exhibits an enhanced diffusive motion with increasing histamine concentration. At a concentration of histamine (500 mM), the motor speed reaches 9.25±1.71 μm.s−1, which is 2.3 times that of Brownian motion, and the corresponding diffusion coefficient is enhanced by 137%. This novel enzymatic nanomotor system not only expands the physiologic enzyme library for biomedical micro/nanomotors but also provides new ideas for the immune therapy of diseases targeting histamine.

A novel self-cleaning electrochemical biosensor integrating copper porphyrin-derived metal-organic framework nanofilms, G-quadruplex, and DNA nanomotors for achieving cyclic detection of lead ions

A self-cleaning electrochemical biosensor based on two-dimensional Cu-porphyrin (Cu-TCPP) metal-organic framework nanofilms, novel super G-quadruplex (G4), and DNA nanomotors was developed for the cyclic detection of Pb2+ ions. The Cu-TCPP framework with inherent peroxidase activity can create an ultra-thin nanofilm that functioned as a carrier to support the metastable G4 comprising four individual DNA strands. The introduction of Pb2+ and the intercalation of hemin can help it to form stable G4-hemin DNAzymes, which exhibits strong catalytic H2O2 reduction activity, and its number will be directly related to the amount of the introduced Pb2+. Moreover, a DNA nanomotor system is introduced to achieve cyclic detection, and the addition of the fuel DNA strands enables G4 to perform a “complete-dissociation-complete” process for achieving self-cleaning of the electrode interface and the cycle detection of Pb2+. The synergistic effects of Cu-TCPP and G4-hemin DNAzymes, which exhibits efficient and catalytic H2O2 reduction, enhance the performance of the electrochemical sensing system. The linear range of this sensor to Pb2+ is 5 nM–5 μM, and the detection limit is 1.7 nM. Compared with the best system in reported studies, its linear range is five times wider and its detection limit is lower than the previously lowest one. Taking advantage of the Pb2+ stabilized G4, the proposed sensor can selectively detect Pb2+ in the presence of other metal ions. The results presented herein comprise a valuable reference for constructing DNA nanoelectronic devices and establish sensitive and cyclic detection of the target and preparing of self-cleaning electrode interfaces.

Plasmon‐Enhanced, Self‐Traced Nanomotors on the Surface of Silicon

AbstractLight‐driven nanomotors have attracted much attention due to their potential applications. The movement of conventional nanomotors typically occurs in the solution phase, which limits their application fields. Utilizing visible light to drive nanomotors at the solid–liquid interface represents a grand challenge due to the large friction force between the nanomotor and the solid surface. Based on the attractive plasmon resonance of Au nanocrystals, for the first time, plasmon‐enhanced Au nanocrystal‐based nanomotors moving at the silicon–aqueous solution interface have been developed. Such nanomotors move with a clear trace engraved on the Si surface, representing an excellent and exceptional self‐traced nanomotor system. In addition, the nanomotor trace on the Si surface also provides a unique and promising approach to the fabrication of nanoscale Si patterns, which is central to many applications, including microelectronics, sensing, information storage, and optoelectronics.

Plasmon-Enhanced, Self-Traced Nanomotors on the Surface of Silicon.

Light-driven nanomotors have attracted much attention due to their potential applications. The movement of conventional nanomotors typically occurs in the solution phase, which limits their application fields. Utilizing visible light to drive nanomotors at the solid-liquid interface represents a grand challenge due to the large friction force between the nanomotor and the solid surface. Based on the attractive plasmon resonance of Au nanocrystals, for the first time, plasmon-enhanced Au nanocrystal-based nanomotors moving at the silicon-aqueous solution interface have been developed. Such nanomotors move with a clear trace engraved on the Si surface, representing an excellent and exceptional self-traced nanomotor system. In addition, the nanomotor trace on the Si surface also provides a unique and promising approach to the fabrication of nanoscale Si patterns, which is central to many applications, including microelectronics, sensing, information storage, and optoelectronics.

Apoptotic Tumor DNA Activated Nanomotor Chemotaxis.

Inspired by the tactic organisms in Nature that can self-direct their movement following environmental stimulus gradient, we proposed a DNase functionalized Janus nanoparticle (JNP) nanomotor system for the first time, which can be powered by ultralow nM to μM levels of DNA. The system exhibited interesting chemotactic behavior toward a DNA richer area, which is physiologically related with many diseases including tumors. In the presence of the subtle DNA gradient generated by apoptotic tumor cells, the cargo loaded nanomotors were able to sense the DNA signal released by the cells and demonstrate directional motion toward tumor cells. For our system, the subtle DNA gradient by a small amount (10 μL) of tumor cells is sufficient to induce the chemotaxis behavior of self-navigating and self-targeting ability of our nanomotor system, which promises to shed new light for tumor diagnosis and therapy.

MnO2-Based Nanomotors with Active Fenton-like Mn2+ Delivery for Enhanced Chemodynamic Therapy.

Chemodynamic therapy (CDT) is an emerging strategy for cancer treatment based on Fenton chemistry, which can convert endogenous H2O2 into toxic ·OH. However, the limited endocytosis of passive CDT nanoagents with low penetrating capability resulted in unsatisfactory anticancer efficacy. Herein, we propose the successful fabrication of a self-propelled biodegradable nanomotor system based on hollow MnO2 nanoparticles with catalytic activity for active Fenton-like Mn2+ delivery and enhanced CDT. Compared with the passive counterparts, the significantly improved penetration of nanomotors with enhanced diffusion is demonstrated in both the 2D cell culture system and 3D tumor multicellular spheroids. After the intracellular uptake of nanomotors, toxic Fenton-like Mn2+ is massively produced by consuming overexpressed intracellular glutathione (GSH), which has a strong scavenging effect on ·OH, thereby leading to enhanced cancer CDT. The as-developed MnO2-based nanomotor system with enhanced penetration and endogenous GSH scavenging capability shows much promise as a potential platform for cancer treatment in the near future.

Micro‐/Nanomotors: Micro‐/Nanomotors toward Biomedical Applications: The Recent Progress in Biocompatibility (Small 27/2020)

In article number 1906184, Yingfeng Tu, Fei Peng, and co-workers highlight the recent progress of biocompatible micro-/nanomotor systems and their applications in the biomedical field. By summarizing the effort that has been made for the biocompatibility of micro-/nanomotors during the past decade, it is expected that the real-world application of micro-/nanomotors in biomedicine will be further developed.

Micro-/Nanomotors toward Biomedical Applications: The Recent Progress in Biocompatibility.

Inspired by the highly versatile natural motors, artificial micro-/nanomotors that can convert surrounding energies into mechanical motion and accomplish multiple tasks are devised. In the past few years, micro-/nanomotors have demonstrated significant potential in biomedicine. However, the practical biomedical applications of these small-scale devices are still at an infant stage. For successful bench-to-bed translation, biocompatibility of micro-/nanomotor systems is the central issue to be considered. Herein, the recent progress in micro-/nanomotors in biocompatibility is reviewed, with a special focus on their biomedical applications. Through close collaboration between researches in the nanoengineering, material chemistry, and biomedical fields, it is expected that a promising real-world application platform based on micro-/nanomotors will emerge in the near future.

Light-Guided Nanomotor Systems for Autonomous Photothermal Cancer Therapy

Machines have greatly contributed to the human civilization, enabling tasks beyond our capacities for improved quality of life. Recently, the progress in nanotechnology has triggered to build a miniaturized machine of nanoscale. In this context, synthetic nanomotors have gained considerable interest because of their great promise for diverse applications. Currently, the movement control of these nanomotors has been widely investigated using various stimuli. Here, we demonstrate near-infrared (NIR) light controlled on/off motion of stomatocyte nanomotors powered by the conversion of hydrogen peroxide. The nanomotors encapsulating naphthalocyanine (NC) are aggregated or separated (collective motion) with or without near-IR light illumination, resulting in the well-controlled movement. Remarkably, the nanomotors can move directionally toward hydrogen peroxide released from cancer cells and photothermally ablate the cancer cells. Taken together, our stomatocyte nanomotor systems can be effectively harnessed for autonomous photothermal cancer therapy.

Internally/Externally Bubble-Propelled Photocatalytic Tubular Nanomotors for Efficient Water Cleaning.

We describe a highly effective bubble-propelled nanomotor for the photocatalytic decomposition of organic pollutants in water. Two different tubular TiO2 nanomotor systems are presented: one with Pt nanoparticles decorated on the inner surface and the other with Pt nanoparticles decorated on the outer surface. This is the first time that we have observed the autonomous movement of a tubular nanomotor without the aid of any surfactant, as well as a tubular nanomotor externally decorated with Pt propelled by oxygen bubbles. The synergy between the Pt nanoparticles and the superhydrophilic wetting behavior of the TiO2 nanotubes endows the two nanomotor systems with high speed at very low H2O2 fuel concentrations without the addition of any surfactant. The efficient photodecomposition of rhodamine B demonstrates the intermixing and photocatalytic ability of the two nanomotor systems, which opens new avenues for the development of multifunctional bubble-propelled micro/nanomotors with myriad practical applications.

Redox-Sensitive Stomatocyte Nanomotors: Destruction and Drug Release in the Presence of Glutathione.

The development of artificial nanomotor systems that are stimuli‐responsive is still posing many challenges. Herein, we demonstrate the self‐assembly of a redox‐responsive stomatocyte nanomotor system, which can be used for triggered drug release under biological reducing conditions. The redox sensitivity was introduced by incorporating a disulfide bridge between the hydrophilic poly(ethylene glycol) block and the hydrophobic polystyrene block. When incubated with the endogenous reducing agent glutathione at a concentration comparable to that within cells, the external PEG shells of these stimuli‐responsive nanomotors are cleaved. The specific bowl‐shaped stomatocytes aggregate after the treatment with glutathione, leading to the loss of motion and triggered drug release. These novel redox‐responsive nanomotors can not only be used for remote transport but also for drug delivery, which is promising for future biomedical applications.

Redox‐Sensitive Stomatocyte Nanomotors: Destruction and Drug Release in the Presence of Glutathione

AbstractThe development of artificial nanomotor systems that are stimuli‐responsive is still posing many challenges. Herein, we demonstrate the self‐assembly of a redox‐responsive stomatocyte nanomotor system, which can be used for triggered drug release under biological reducing conditions. The redox sensitivity was introduced by incorporating a disulfide bridge between the hydrophilic poly(ethylene glycol) block and the hydrophobic polystyrene block. When incubated with the endogenous reducing agent glutathione at a concentration comparable to that within cells, the external PEG shells of these stimuli‐responsive nanomotors are cleaved. The specific bowl‐shaped stomatocytes aggregate after the treatment with glutathione, leading to the loss of motion and triggered drug release. These novel redox‐responsive nanomotors can not only be used for remote transport but also for drug delivery, which is promising for future biomedical applications.

Nanomotor-Enabled pH-Responsive Intracellular Delivery of Caspase-3: Toward Rapid Cell Apoptosis.

Direct and efficient intracellular delivery of enzymes to cytosol holds tremendous therapeutic potential while remaining an unmet technical challenge. Herein, an ultrasound (US)-propelled nanomotor approach and a high-pH-responsive delivery strategy are reported to overcome this challenge using caspase-3 (CASP-3) as a model enzyme. Consisting of a gold nanowire (AuNW) motor with a pH-responsive polymer coating, in which the CASP-3 is loaded, the resulting nanomotor protects the enzyme from release and deactivation prior to reaching an intracellular environment. However, upon entering a cell and exposure to the higher intracellular pH, the polymer coating is dissolved, thereby directly releasing the active CASP-3 enzyme to the cytosol and causing rapid cell apoptosis. In vitro studies using gastric cancer cells as a model cell line demonstrate that such a motion-based active delivery approach leads to remarkably high apoptosis efficiency within a significantly shorter time and with a lower amount of CASP-3 compared to other control groups not involving US-propelled nanomotors. For instance, the reported nanomotor system can achieve 80% apoptosis of human gastric adenocarcinoma cells within only 5 min, which dramatically outperforms other CASP-3 delivery approaches. These results indicate that the US-propelled nanomotors may act as a powerful vehicle for cytosolic delivery of active therapeutic proteins, which would offer an attractive means to enhance the current landscape of intracellular protein delivery and therapy. While CASP-3 is selected as a model protein in this study, the same nanomotor approach can be readily applied to a variety of different therapeutic proteins.

A nanoengine governor based on the end interfacial effect

A conceptual design is presented for a nanoengine governor based on the end interfacial effect of two rotary nanotubes. The governor contains a thermal-driven rotary nanomotor made from double-walled carbon nanotubes (DWCNTs) and a coaxially laid out rotary nanotube near one end of the nanomotor rotor. The rotation of the rotor in the nanomotor can be controlled by two features. One is the stator (the outer tube of DWCNTs) which has some end atoms with inward radial deviation (IRD) on the stator. The other is the relative rotation of the neighboring rotary tube of the rotor. As the configuration of the stator is fixed, the end interfacial interaction between the two rotors will govern the dynamic response of the rotor in the nanomotor system. The obtained results demonstrate that the relative rotational speed between the two rotors provides friction on the rotor in the nanomotor system. In particular, higher relative rotational speed will provide lower friction on rotor 1, which is opposite to that between neighboring shells in DWCNTs.

Ultrahigh-speed rotating nanoelectromechanical system devices assembled from nanoscale building blocks

The development of rotary nanomotors is crucial for advancing nanoelectromechanical system technology. In this work, we report design, assembly and rotation of ordered arrays of nanomotors. The nanomotors are bottom-up assembled from nanoscale building blocks with nanowires as rotors, patterned nanomagnets as bearings and quadrupole microelectrodes as stators. Arrays of nanomotors rotate with controlled angle, speed (over 18,000 r.p.m.), and chirality by electric fields. Using analytical modelling, we reveal the fundamental nanoscale electrical, mechanical and magnetic interactions in the nanomotor system, which excellently agrees with experimental results and provides critical understanding for designing metallic nanoelectromechanical systems. The nanomotors can be continuously rotated for 15 h over 240,000 cycles. They are applied for controlled biochemical release and demonstrate releasing rate of biochemicals on nanoparticles that can be precisely tuned by mechanical rotations. The innovations reported in this research, from concept, design and actuation to application, are relevant to nanoelectromechanical system, nanomedicine, microfluidics and lab-on-a-chip architectures.

Complete ON/OFF Photoswitching of the Motility of a Nanobiomolecular Machine

To apply motor proteins as natural nanomolecular machines to transporting systems in nanotechnology, complete temporal control over ON/OFF switching of the motility is necessary. We have studied the photoresponsive inhibition properties of azobenzene-tethered peptides for regulation of kinesin-microtubule motility. Although a compound containing a peptide having an amino acid sequence derived from the kinesin's C-terminus (a known inhibitor of kinesin's motor domain) and also featuring a terminal azobenzene unit exhibited an inhibition effect, the phototunability of this behavior upon irradiation with UV or visible light was only moderate. Unexpectedly, newly synthesized peptides featuring the reverse sequence of amino acids of the C-terminus of kinesin exhibited excellent photoresponsive inhibition. In particular, azobenzene-CONH-IPKAIQASHGR completely stopped and started the motility of kinesin-microtubules in its trans- and cis-rich states, respectively, obtained after irradiation with visible and UV light, respectively. A gliding motility system containing this photoresponsive inhibitor allowed in situ control of the motion of microtubules on a kinesin-coated glass substrate. It is expected that the present results on the photoresponsive nanomotor system open up new opportunities to design nanotransportation systems.

Autonomous Synergic Control of Nanomotors

Control is a hallmark of machines; effective control over a nanoscale system is necessary to turn it into a nanomachine. Nanomotors from biology often integrate a ratchet-like passive control and a power-stroke-like active control, and this synergic active-plus-passive control is critical to efficient utilization of energy. It remains a challenge to integrate the two differing types of control in rationally designed nanomotor systems. Recently a light-powered track-walking DNA nanomotor was developed from a bioinspired design principle that has the potential to integrate both controls. However, it is difficult to separate experimental signals for either control due to a tight coupling of both controls. Here we present a systematic study of the motor and new derivatives using different fluorescence labeling schemes and light operations. The experimental data suggest that the motor achieves the two controls autonomously through a mechanics-mediated symmetry breaking. This study presents an experimental validation for the bioinspired design principle of mechanical breaking of symmetry for synergic ratchet-plus-power stroke control. Augmented by mechanical and kinetic modeling, this experimental study provides mechanistic insights that may help advance molecular control in future nanotechnological systems.