Murraya Paniculata Research Articles

Knockdown of calreticulin, laccase, and Snf7 Genes Through RNAi Is Not Effective to Control the Asian Citrus Psyllid (Hemiptera: Livideae).

The Asian citrus psyllid, Diaphorina citri Kuwayama, transmits the bacteria Candidatus Liberibacter associated with huanglongbing (HLB), a devastating disease of the citrus industry. The use of genetically modified plants is an alternative to control this vector. Conversely, technology based on RNA interference (RNAi) for silencing specific genes of a target insect could be attempted. This work evaluated the knockdown effect of the target genes calreticulin (DcCRT), laccase (DcLAC), and Snf7 (DcSnf7) by RNAi through feeding D. citri in Murraya paniculata leaves after the uptake of an aqueous solution with dsRNA homologous to each vector target gene. Confocal microscopy revealed the uptake of the fluorescent-labeled dsRNA by detached leaves and the symplastic movement, allowing the ingestion by the feeding insect. A reduction in the survival rate was observed only 144 h after the beginning of feeding with dsRNA targeting DcSnf7; however, no reduction in transcript accumulation. The knockdown of the DcCRT and DcLAC genes was detected only 12 and 96 h after insect feeding, respectively. Additionally, a reduction in amino acid excretion from insects fed with dsRNA targets to DcCRT and DcLAC was observed 120 h after the beginning of feeding. However, the effects of the dsRNAs tested here appear to be minimal, both at the transcriptional and phenotype levels. For most concentrations and time points, no effects were observed. Therefore, the knockdown of genes DcCRT, DcLAC, and DcSnf7 do not appear to have the potential to control of D. citri through RNAi-mediated gene silencing.

Seasonal foliar uptake of atmospheric polycyclic aromatic hydrocarbons by some local plants in a tropical metropolis in India

This study explored the interspecies and seasonal variation of polycyclic aromatic hydrocarbons (PAHs) in the extracted lipids of the leaves of seven local plants in an urban environment of Kolkata (22°33′N and 88°20′E), India. Based on the degree of toxicity and carcinogenicity (expressed in terms of their Benzo(a)pyrene equivalent (BaPeq) concentrations) the overall foliar-PAH accumulation during the study period (September 2018‒;August 2019) in the various plants showed the following order: Nerium oleander (80.96 ± 30.08 ng.gdw−1) > Mangifera indica (74.15 ± 20.34 ng.gdw−1) > Lantana aculeata (60.13 ± 21.71 ng.gdw−1) > Thevetia peruviana (40.97 ± 12.45 ng.gdw−1) > Ixora coccinea (38.11 ± 9.5 ng.gdw−1) > Murraya paniculata (37.1 ± 7.35 ng.gdw−1) > Polyalthia longifolia (25.72 ± 5.71 ng.gdw−1). The PAHs like phenanthrene, anthracene, fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo (b+k)fluoranthene, benzo(a)pyrene, benzo [ghi]perylene and indeno [1,2,3-cd]pyrene were predominant during the study period over the PAHs like naphthalene, acynaphthylene, acenaphthene, fluorine and dibenz [a,h]anthracene in the extracted lipids. The temperature-dependent partitioning of the PAHs onto leaf-surface and photo-degradation could have affected the availability of the PAHs. The foliar PAH accumulation varied seasonally as winter (December–February) > postmonsoon (September–November) > premonsoon (March–May) > monsoon (June–August). The leaf epicuticular wax determined the PAH uptake and storage, which in turn was affected by the temperature and solar radiation. In consistence with the idea of “nature-based solutions” for deteriorated air quality remediation in an urban environment, this study could be a promising initiative to build up cost-effective biological filters to combat the airborne pollutants and improve urban air quality.

Cooling effect and control factors of common shrubs on the urban heat island effect in a southern city in China

Because the heat island effect can make cities warmer than their surroundings, it can make urban dwellers uncomfortable and even affect their health, which is particularly pronounced in developed cities in southern China. To reduce the heat island effect and improve the environment, various types of vegetation have been planted in the urban green belt. Though previous studies have been conducted on the beauty, air purification functions and cooling effect of vegetation, little is concentrated on the different cooling effects and control factors of various common shrubs on the heat island effect in cities. In this study, five of the most regionally common shrubs were selected to study the cooling effect in Guangzhou, southern China. The maximum surface temperatures of five shrubs and pavement were compared using infrared temperature sensors from April 1st 2019 to October 31st 2019. Results show that (1) All five shrubs showed noticeable seasonal variation, and the average surface temperatures of the five shrubs were between 38.0 and 42.2 °C during May–August and 30.7–34.1 °C during the other seasons (April, September and October);. (2) Murraya exotica L. exhibited the best cooling effect on the maximum surface temperature. Its value was 44.7 °C, and the absolute difference values of Murraya exotica L. (10.3 ± 1.7 °C) were higher than any other shrub during the study period; (3) Both the LAI (R2 = 0.57, p < 0.01) and plant height (R2 = 0.13, p < 0.01) are control factors of the cooling effect on vegetation surface temperature for the five shrubs. This study revealed the differences in the cooling effect and influencing factors of five regionally common shrubs on the heat island effect. Research on the functional characteristics of plants and plant selection in urban green belts has both theoretical and practical significance.

Effectiveness of the Brown Lacewing, Sympherobius barberi Banks as a Biological Control Agent of the Asian Citrus Psyllid Diaphorina citri Kuwayama.

The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama is an economically important pest of citrus because it vectors the causal pathogens of huanglongbing (HLB) or citrus greening disease. Biological control is an important component of citrus pest management but requires consistent strengthening of its impact on pest complex. The brown lacewing Sympherobius barberi Banks is a known predator of several insect pests from Asia, Europe, and America. However, there is not much information about its effectiveness against D. citri. We evaluated S. barberi against the D. citri and frozen eggs of the Mediterranean flour moth Ephestia kuehniella, the latter is a common diet used for rearing predators in laboratories. Adult S. barberi successfully fed on D. citri eggs and nymphs under both light and dark conditions. Diaphorina citri was also suitable for the development and reproduction of S. barberi except for slightly prolonged larval development compared with E. kuehniella diet. The egg hatch from the total number of eggs laid on D. citri and E. kuehniella diets averaged 65% and 52%, respectively. Females laid 64% eggs on dimpled white paper compared to 36% combined on plain paper and leaves of citrus, orange jasmine, eggplant and cantaloupe. Sympherobius barberi released at densities of 2–6 adults against eggs and nymphs of D. citri on infested orange jasmine plants in the cages provided a reduction of 43–81% in the number of provided eggs or nymphs. In the field tests on D. citri infested citrus trees, reduction averaged 35% in five cohorts in which developing colonies of 28–32 nymphs were provided to one S. barberi per cage. Findings suggest the significant potential of S. barberi as a predator of D. citri and to contribute to reducing huanglongbing.

AKTIVITAS LARVASIDA EKSTRAK ETANOL DAUN KEMUNING (Murraya paniculata JACK) TERHADAP LARVA Aedes aegypti

Introduction: A research had been carried out to analyze the larvicidal activity of the ethanol extract of kemuning leaves (Murraya paniculata (L.) Jack.) against Aedes aegypti L. larvae. Method: The research began with extracting yellow leaves (Murraya paniculata (L.) Jack.) With a modified maceration method using 80% ethanol solvent until a thick extract is obtained, then a preliminary test is carried out to obtain LC5 and LC95 so that 5 concentrations of yellow leaf extract (Murraya paniculata (L.) Jack.) can be obtained which can be used as a real larvicide test. The actual larvicide test was carried out with 7 treatments, namely 1 negative control using Tween 80, 5 test treatments namely 1000 bpj yellow leaf extract, 3000 bpj, 5000 ppm, 7000 ppm and 9000 ppm and 1 positive control using Temephos 2 bpj. Observation of the test results was carried out after 24 hours by counting the number of larvae deaths in each treatment group. Result: The data obtained were analyzed by using probit analysis and obtained LC90 of yellow leaf extract (Murraya paniculata (L.) Jack.) which is 8330,291 bpj. The results showed that the yellow leaf extract (Murraya paniculata (L.) Jack.) was effective as a larvicidal activity with LC5 of 1000 bpj and LC95 of 9000 bpj.

Polymethoxylated flavonoids from Murraya paniculata (L.) Jack

A new flavone, 3′-hydroxy-5,6,7,4′,5′-pentamethoxyflavone (1), and three natural products (7, 10, and 14) were isolated from the leaves and twigs of Murraya paniculata (L.) Jack, together with 10 reported polymethoxylated flavonoids. The structure of compound 1 was elucidated on the basis of comprehensive analysis of 1D and 2D NMR and HRMS spectroscopic data. Moreover, the chemotaxonomic significance of these compounds was also discussed.

Presence and abundance of Diaphorina citri in Murraya paniculata in urban areas free of huanglongbing in Brazil

AbstractAsian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), is the vector of the bacterium Candidatus Liberibacter sp., a quarantine pathogen in citrus production areas such as Australia, Europe, and northeast Brazil, associated with huanglongbing (HLB). The psyllids’ preferred host is Murraya paniculata (L.) Jacq. (Rutaceae), an ornamental plant native to Asia and Oceania, and commonly found in urban areas next to citrus groves around the world. As there are insecticide application restrictions for urban areas, monitoring and use of biological control to suppress ACP are essential for an area‐wide HLB management strategy, especially for production areas predominantly composed of small citrus farms. For this, it is necessary to understand the occurrence cycles of ACP and vegetative flush of the ornamental host. This study characterized the occurrence cycles of M. paniculata flush shoot and accumulated rainfall, and the association with ACP presence (proportion of monitored trees) and abundance (per tree) in a Brazilian urban area free of HLB. A 3‐year time series was constructed using spectral and co‐spectral analysis. Spectral analysis showed the occurrence of at least seven flush shoot cycles of M. paniculata, nearly 10 ACP presence and abundance cycles, and monthly cycles of accumulated rainfall. Cycles of ACP presence were associated with ACP abundance, with ACP presence cycles occurring around 7 days before ACP abundance cycles and the correlation and co‐spectral analyses indicated an almost simultaneous occurrence of the main cycles of M. paniculata flush shoots and ACP occurrence. These findings will facilitate the development of an ACP biological control program based on parasitoid releases in urban areas in HLB‐free citrus groves.

Pemanfaatan Daun Kemuning Sebagai Obat Tradisional Penyakit Asma

Kemuning (Murraya paniculata L) is a shrub growing in tropical climates and wildly in bushland or forest. The local people of Serawai ethnicin Bengkulu often use kemuning leaves as a traditional medicine for asthma disease. . This study aims to analyze the potential and benefits of Kemuning (Muraya paniculata L) leaves as a traditional medicine forasthma disease. This research is a descriptive study by using interview method with Snow ball sampling techniques . The results showed that Murraya paniculata leaves have been empirically proven to treat asthma in Serawai tribe community. The leaves are processed by squeezing , filtering, then drinking or smear ing it on the chest. The presence of flavonoids in Murraya paniculata leaves thought have the effect of reducing asthma. Murraya paniculata leaves have potential to be developed as an asthma medicine , but further research is need ed before us ing in the pharmaceutical industry.

Antiplasmodial and Antipyretic Activity and Safety Evaluation of the Methanolic Leaf Extract of Murraya exotica (L.).

Background The increasing mortality and morbidity of malaria in Africa coupled with the recent reports of antimalarial drug resistance reinforces the need for novel antimalarial agents from natural plant products with folkloric use for the disease. Murraya exotica (L.) (Rutaceae) is widely used as an ornamental plant used indigenously to treat fever, cough, and infectious wounds and eliminate pain from injury and trauma. This study was conducted to evaluate extracts of the leaves of Murraya exotica (L.) (Rutaceae) for its safety and antipyretic and antimalarial activity in rodent models. Method In this study, the Peters 4-day suppressive and curative test in Plasmodium berghei-infected mice was used to demonstrate the antiplasmodial activity of the methanolic leaf extract of Murraya exotica (L.) (MEE). The study also evaluated the subacute toxicity study and the antipyretic activity of MEE on baker's yeast-induced hyperthermia in rodent models. Results Murraya exotica (L.) extract demonstrated curative antimalarial activity, with a percentage suppression of 45.84, 64.32 ± 0.33, 56.74 ± 2.16, and 64.61 ± 0.67 at doses of 50, 100, 300, and 600 mg/kg, respectively. In the Peters 4-day suppressive test, MEE at dose 600 mg/kg had the highest chemosuppression (76.02 ± 1.38%) compared with artesunate (2 mg/kg, p.o.) (82.56 ± 0.97%). Subacute oral toxicity studies in Sprague-Dawley rats documented no deaths, with no significant changes in clinical signs, organ weights, and hematological and biochemical parameters. The LD50 of MEE was estimated to be above 1000 mg/kg in Sprague-Dawley rats. All doses of MEE and paracetamol reduced pyrexia in 1 h and 2 h after their administration. The percentage reduction of rectal temperature (TR) for the positive control (paracetamol, 150 mg/kg, p.o.) was 44.36% while the Murraya exotica extract at doses 50 mg/kg, 100 mg/kg, 300 mg/kg, and 600 mg/kg recorded 67.74%, 40.78%, 66.42%, and 59.42%, respectively. Murraya exotica at dose 100 mg/kg exhibited significant reduction (p < 0.05) in baker's yeast-induced pyrexia. Conclusions The findings in this study show the antipyretic, curative, and suppressive antiplasmodial activity as well as the safety of the methanolic leaf extract of Murraya exotica (L.) supporting its traditional use for malaria and fever.

First Report of HLB Causal Agent in Psyllid in State of Bahia, Brazil.

The state of Bahia ranks fourth in the national rank for citrus production, and the region of Chapada Diamantina is emerging an important producer of orange for fresh fruit market. Huanglongbing (HLB) is the major phytosanitary threat to Bahia citriculture. In Brazil, the disease was first reported in 2004 in São Paulo state. The bacterium Candidatus Liberibacter asiaticus (CLas) is one of the causal agents of HLB, which is transmitted by the insect vector Diaphorina citri Kuwayama (Hemiptera: Liviidae). Bahia is a HLB-free area; therefore, it is essential to monitor its citrus-producing areas to early detect any possible introduction of the CLas. This study aimed to monitor the presence of the bacteria in the insect vector. Diaphorina citri samples were collected from 2011 to 2014 in different cities located at Chapada Diamantina region and tested by qPCR for the presence of CLas. Three samples were considered positive to bacterium, and all from psyllids collected on Murraya paniculata in the city of Seabra.

Coumarin derivatives from the leaves and twigs of Murraya exotica L. and their anti-inflammatory activities

Fifteen previously undescribed coumarin derivatives, murratins A−M, including two pairs of coumarin enantiomers with a cyclopropane unit, two benzocoumarins, a dimeric coumarin, and eight C-8-substituted coumarins, together with six known analogues were isolated from the extract of the leaves and twigs of Murraya exotica L., a medicinal plant named “Jiulixiang” in Chinese. Their structures were elucidated on the basis of comprehensive analysis of 1D and 2D NMR and HRMS spectroscopic data, and the absolute configurations were assigned via comparison of the specific rotations, the ECD exciton coupling method, comparison of experimental and calculated ECD data, and the ECD data of in situ formed transition metal complexes. All the isolates were evaluated for their inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells, and five coumarin derivatives showed moderate inhibitory activities. The possible mechanism for NO inhibition of undescribed bioactive compounds was deduced to interact with iNOS protein via molecular docking. The above results shed some light on a better understanding of the traditional anti-inflammation effect of M. exotica and coumarins are disclosed to be its potential anti-inflammatory constituents.

Phytochemical Study and Antioxidant Properties of Aqueous Extracts of Murraya paniculata Leaf

Aims: To investigate the chemical groups present, total phenolic compound, total flavonoid and to evaluate the possible antioxidant activity of aqueous extracts of Murraya paniculata leaf.&#x0D; Place and Duration of Study: Department of Applied Chemistry and Chemical Engineering Department, Noakhali Science and Technology University, Bangladesh, in 2017&#x0D; Methodology: The dry leaves were boiled and extracted with water. The extracts were concentrated and then investigated for their total phenolic compound, total flavonoid, evaluated the antioxidant activity. The preliminary screening of the various extracts was carried out using standard methods. Total phenolic content (TPC) was determined by the modified Folin-Ciocalteu method, total antioxidant capacity (TAC) and total flavonoid content were measured according to the Phosphomolybdate-method and Dowd-method respectively.&#x0D; Results: The aqueous extract showed good antioxidant activity with good phenolic and flavonoid contents (400±0.44 mg ascorbic acid equivalent/g dried weight, 263±0.62 mg gallic acid equivalent/g dried weight, 63±0.19 mg quercetin equivalent/g dried weight respectively).&#x0D; Conclusion: The research work revealed the presence of various bioactive phytochemical compounds which show the medicinal importance of leaves Murraya paniculata through a facile extracting method.

IDENTIFICACIÓN Y EVALUACIÓN DE LA INCIDENCIA DE INSECTOS Y HONGOS BENÉFICOS ASOCIADOS A Diaphorina citri KUWAYAMA (HEMIPTERA: LIVIIDAE) EN PLANTAS TRASPATIO (Citrus spp. y Murraya paniculata) DEL CANTÓN CATAMAYO (LOJA - ECUADOR)

En los últimos años, la citricultura mundial se ha encontrado amenazada por la presencia del psílido asiático de los cítricos, Diaphorina citri, un insecto perjudicial por ser portador de la bacteria “Candidatus Liberibacter asiaticus (las)”, causante de la enfermedad Huanglongbing (HLB), que ha provocado la devastación de plantaciones y la reducción de la producción de cítricos en todo el mundo. En el año 2017 se reportó la presencia de D. citri en plantas traspatio del cantón Catamayo, en la provincia de Loja, por lo que la presente investigación tuvo por objetivo identificar los enemigos naturales (depredadores, parasitoides y hongos) asociados a D. citri, y evaluar su incidencia en campo. Para ello, se realizaron muestreos en plantas traspatio (Citrus spp. y Murraya paniculata) del cantón Catamayo. Los muestreos se efectuaron a una altura de 1,5 m de las plantas en los cuatro puntos cardinales de la misma. Se colectaron larvas y adultos de depredadores, ninfas parasitadas y adultos de parasitoides, y adultos de D. citri para el aislamiento de hongos. Como resultado de la identificación en el laboratorio, se determinó la presencia de 11 especies depredadoras (principalmente Cheilomenes sexmaculata y Chrysopa spp.), dos especies de parasitoides (Diaphorencyrtus sp. y Tamarixia radiata) y cuatro especies de hongos (Cladosporium sp., Fusarium sp., Aspergillus sp. y Alternaria sp.). Diaphorencyrtus sp. tuvo una tasa de parasitismo del 7,76 %. Estas especies podrían utilizarse para establecer programas de control biológico, aprovechando su potencial como reguladores de poblaciones de ninfas de Diaphorina citri.

Formulasi dan Evaluasi Sifat Fisik Lotion Ekstrak Etanol Daun Kemuning (Murraya paniculata (L) Jack) dan Daun Lidah Buaya (Aloe vera Linn)

The ultraviolet radiation and skin water loss could damage the skin. Kemuning leaves (Murraya paniculata (L) Jack) as a source of anti-oxidants and Aloe vera leaves (Aloe vera Linn) can prevent this action. This study aims to formulate and evaluate lotion containing the Kemuning leaves and Aloe vera extract. The extract of Kemuning leaves was obtained by sonication using 70% ethanol solvent. Aloe vera juice was obtained by smoothing the aloe vera gel then filtered using clean gauze. The kemuning leaves extract and aloe vera extract had formulated into lotion preparation. The physical properties of the lotion (homogeneity, type of emulsion, pH, spreadability, and adhesion) were determined. The organoleptic test showed that the color of the lotion was pale yellow with a smooth texture and had extract scent. The lotion was showed good homogeneity, spreadability 7.31 cm, pH 7, type oil in water and adhesion 45 seconds. The physical properties parameters of lotion preparations showed that the lotion preparations had fulfilled the requirements of good preparations.

La Dispersión del Psílido Asiático, Diaphorina citri Kuwayama y su Parasitoide, Tamarixia radiata (Waterston) en la Provincia de Manabí, Ecuador

The Asian psyllid, Diaphorina citri, is a potential problem for citrus farming in Ecuador due to the transmission of Huanlongbing, a devastating bacterial disease. Although the disease has not been reported, D. citri was detected in 2013 in Ecuador in the province of Guayas. It was observed in 2016 in the province of Manabí, on an orange jessamine tree, Murraya paniculata. Additionally, its parasitoid, Tamarixia radiata has not been referred to Manabí. To document the dispersion of the insect and parasitoid in the region, during May - December 2018, M. paniculata and citrus trees were sampled in 107 production units of Portoviejo and Santa Ana, where the phytophage and its parasitoid were detected on M. paniculata (since May 2018) and citrus only the phytophage was observed (since November 2018). Results suggest the establishment of D. citri and T. radiata on M. paniculata and its recent colonization on citrus.

In vitro antileishmanial and antioxidant activities of essential oils from different parts of Murraya paniculata (L.) Jack: a species of Rutaceae that occur in the Cerrado biome in Brazil

In Brazilian folk medicine, Murraya paniculata has been used for treating intestine disorders, rheumatism and cough. This paper aims to investigate the in vitro antileishmanial and antioxidant activities of essential oils (EO) from M. paniculata leaves and fruits (ripe and unripe ones). Natural antioxidants may be very beneficial to improve quality of life, since they are capable of protecting the body against damage caused by free radicals and, consequently, either preventing or postponing many diseases from starting their cycles. One of the techniques which has been widely used for detecting antioxidant compounds is the method based on the elimination of the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). It has been considered easy, precise, fast, simple, economical and appropriate to determine antioxidant activity of pure substances and complex mixtures, such as EO. Thus, antioxidant potential of EO was evaluated by using the method of the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). EO from M. paniculata leaves and fruits exhibited weak potential, since EC50 values were above 700 µg/mL. Several reports have stated that volatile oils from plants show promising leishmanicidal activity against promastigote forms of Leishmania amazonensis; in relation to this activity, leaf oil was highly active (IC50 = 7.33±2.07 µg/mL) while ripe and unripe fruit oils were active, with values of IC50 = 30.77±2.07 µg/mL and 13.04±1.64 µg/mL, respectively. Both GC-FID and GC-MS analyses revealed that the major components determined in EO from M. paniculata were sesquithujene (25.0%), trans-β-caryophyllene (23.8%), α-zingiberene (21.0%), α-ylangene (13.3%), germacrene D (13.1%), α-copaene (12.7%), and β-cubebene (10.2%). In vitro antileishmanial and antioxidant activities of EO from M. paniculata have also been described for the first time.

Seasonal and diel variations in scent composition of ephemeral Murraya paniculata (Linn.) Jack flowers are contributed by separate volatile components

Scent profile of the ephemeral nocturnal floral species Murraya paniculata was characterized to assess diel and seasonal variability. The floral fragrance was found to constitute a characteristic set of 15 major volatile organic compounds (VOCs) dominated by benzenoids, terpenoids and phenylethanoids. Strong diel variability was detected due to dominantly endogenous regulation of 2-phenylethanol and (E)-β-ocimene emission levels, along with temperature-correlated emission rates of 2-phenylethanal, methyl benzoate, methyl anthranilate and germacrene D, resulting in high diurnal contents of the former two VOCs and high nocturnal contents of the latter four VOCs in the fragrance. Significant seasonal variation was contributed by a separate suite of VOCs including linalool, nonanal, decanal, methyl salicylate and methyl palmitate. Higher abundances of these components in certain seasons were found to add subtle variations in the strong diurnal/nocturnal division in the scent profile. Emission rate of linalool is endogenously regulated on a seasonal scale, but that of (E,E)-α-farnesene was maintained at consistent proportions even if correlated to temperature variations. It was concluded that the two sub-sets of major scent compounds, each comprising six VOCs, are adaptive responses such that the strong day/night component of variation in maintained along with necessary physiological adjustments to changing seasons. The varying effects of climatic factors on the 15 major VOCs indicated different regulating mechanisms comprising environmental and endogenous mechanisms in different degrees.

Detection of Phenylpropanoids in Citrus Leaves Produced in Response to Xanthomonas citri subsp. citri.

Citrus canker (CC), caused by the bacterial pathogen Xanthomonas citri subsp. citri, impacts citrus production in many areas of the world by reducing yields, degrading tree health, and severely blemishing the outer peels of fresh fruit. The relative susceptibility to CC among different species of Citrus varies from the highly susceptible lime (Citrus × aurantifolia), sweet orange (C. × sinensis), and grapefruit (C. × paradisi) to the much less susceptible calamondin (C. × microcarpa) and kumquat (C. japonica). This investigation compares the responses to infection with X. citri subsp. citri of these five genotypes with respect to phenylpropanoid compound profiles and relative increases or decreases of specific compounds postinoculation. In response to X. citri subsp. citri infection, all hosts possessed increased concentrations of phenylpropanoids in leaf tissue, whereas the similarly treated nonhost orange jessamine (Murraya paniculata) did not. Several of the tested genotypes exhibited notably increased production of fluorescent phenylpropanoids, including umbelliferone, herniarin, auraptene, scoparone, and others. The profiles of these compounds and their levels of production varied among the tested species yet all investigated Citrus genotypes exhibited increased concentrations of phenylpropanoids regardless of their degree of susceptibility to X. citri subsp. citri. Kumquat and calamondin, the tested genotypes least susceptible to X. citri subsp. citri, also exhibited the highest levels of the dihydrochalcone 3',5'-di-C-glucosyl phloretin, the aglycone portion of which, phloretin, is a known antibiotic, although levels of this compound were not affected by inoculation with X. citri subsp. citri.

Chemical composition and in vitro antibacterial activity of essential oils from Murraya paniculata (L.) Jack (Rutaceae) ripe and unripe fruits against bacterial genera Mycobacterium and Streptococcus

This study aims to investigate chemical composition of essential oils from Murraya paniculata (L.) Jack (Rutaceae) ripe and unripe fruits and determine their in vitro antibacterial activity. Essential oils were extracted by hydrodistillation from Murraya paniculata (L.) Jack ripe and unripe fruits collected in the Cerrado, in Rio Verde, southwestern Goiás, Brazil. They were analyzed by gas chromatography with flame ionization detector (GC-FID) and by gas chromatography-mass spectrometry (GC-MS). Sesquiterpenes, which represent the most abundant class of compounds in oils, predominated in both ripe and unripe fruits. Major constituents of essential oils extracted from ripe fruits (RF-EO) were (-caryophyllene (21.3%), (-ylangene (13.3%), germacrene-D (10.9%) and (-zingiberene (9.7%) whereas the ones of unripe fruits (UF-EO) were sesquithujene (25.0%), (-zingiberene (18.2%), germacrene-D (13.1%) and (-copaene (12.7%). In vitro antibacterial activity of essential oils was evaluated in terms of its minimum inhibitory concentration (MIC) values by the broth microdilution method in 96-well microplates. Both essential oils under investigation showed moderate anti-streptococcal activity against the following bacteria: Streptococcus mutans, S. mitis, S. sanguinis, S. sobrinus and S. salivarius. MIC values ranged between 100 and 400 µg/mL. Regarding the antimycobacterial activity, essential oils from M. paniculata (L.) Jack unripe and ripe fruits were active against Mycobacterium kansasii (MIC = 250 µg/mL), moderately active against M. tuberculosis (MIC = 500 µg/mL) and inactive against M. avium (MIC = 2000 µg/mL). This study was pioneer in revealing similar chemical profiles of both essential oils extracted from Murraya paniculata (L.) Jack unripe and ripe fruits, besides describing their in vitro anti-streptococcal and antimycobacterial activities.

Antibacterial and Antifungal Activity of Flower extract of Murraya paniculata L.

Antibacterial and Antifungal Activity of Flower extract of <i>Murraya paniculata</i> L.