Morphology Of AgNPs Research Articles

Green synthesis: characterization and biological activity of silver nanoparticles using aqueous extracts of plants from the Arecaceae family

This study proposes the preparation, characterization, and evaluation of the antimicrobial activity of silver nanoparticles (AgNPs). AgNPs were synthesized from the leaf extracts of plants from the Arecaceae family, which are abundant in the Amazon region. AgNPs were characterized using UV/Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), dynamic light scattering (DLS), and their minimum inhibitory concentrations (MIC) against the bacteria Escherichia coli and Staphylococcus aureus. AgNPs presented maximum absorbance between 420 and 430 nm, the mean diameter obtained by DLS ranged from 130.43 to 352.93 nm and the polydispersity index (PdI) ranged from 0.523 to 0.689. The surface charge measured by the Zeta potential was negative and ranged from -17.2 to -26.97 mV. FTIR analysis suggests that the phenolic compounds and/or proteins in the chemical composition of the plants studied may have been responsible for the reduction of Ag+ ions and stabilization of AgNPs. The morphology of AgNPs observed was largely spherical and presented some agglomerates. Transmission electron microscopy analyses showed polydispersed AgNPs without the formation of large agglomerates. The synthesized AgNPs presented homogeneity and rapid bioreduction. The concentration of AgNPs required to eliminate microorganisms by up to 90% was lower for Gram-negative bacteria (2.75 μg mL-1) than for Gram-positive bacteria (21.75 μg mL-1). In addition, AgNPs synthesized from plant species that are native to the Amazon proved to be promising, since they showed excellent antimicrobial activity against microorganisms of clinical interest.

Green synthesis and characterization of silver nanoparticles using Pteris vittata extract and their therapeutic activities.

The bacterial infections have been substantially increasing with higher mortality and new regimens required for their management. The present work deals with the green synthesis of silver nanoparticles (AgNPs) using leaf extract of Pteris vittata at pH 9.0. The AgNPs showed a single absorption peak at 407 nm. The morphology of AgNPs was found to be spherical in shape analyzed by scanning electron micrographs. The X-ray diffraction studies revealed the face-centered cubic structure of AgNPs with a 17-nm average crystallite size. They showed the antimicrobial activity against Pseudomonas aeruginosa, and the cell growth was completely ceased at the minimum inhibitory concentration (MIC); 100 μg/mL, with rapidly decreased cell viability. This bactericidal effect was due to the enhancement of cell permeability caused by cell disruption. The AgNPs lead to show a promising antiquorum-sensing activity by inhibition of toxin protease and pyocyanin in P. aeruginosa by 88% and, 94% respectively, at the sub-MIC concentration (0.25× MIC). These results conclude that the green synthesis of AgNPs shows a promising antimicrobial and antivirulence activity against P. aeruginosa.

Green Synthesis of Silver Nanoparticles Using Mukia Maderaspatana

Nanoparticles are utilized in numerous business applications and it has been discovered that watery silver particles can be diminished by fluid exoact of plant parts to deliver exoemely stable silver nanoparticles in water. On this premise, silver nanoparticles (SNPs) were incorporated utilizing leaf aqueoi separate (LAE) of Mukiamaderaspatana. The acquired particles were broke down by UV-obvious spectrophotometry, checking electron microscopy (SEM), Fourier change infrared (FT-lR) spectroscopy to perceive the morphology of AgNPs. FTlR spectroscopic trials hax’e been performed to group potential bioreducing specialists present in the leaves of the plant.

Electrospun AgNPs-polylactate nanofibers and their antimicrobial applications

Silver nanoparticles (AgNPs) have been one of the most significant nanoparticles due to their interesting characteristics, such as high antimicrobial activity. Thus, they have been applicable for wound dressing, protective textiles and tissue scaffold. In the current study, novel polylactate copolymer was prepared via condensation of lactic acid (LA) and methacrylic acid (MAA) at molar ratio of 95:5, and then the produced copolymer was subjected to free radical polymerization in the presence of N, N-methylene diacrylamide (MDA) as a cross-linking agent. AgNPs were synthesized utilizing chitosan (Cs) as reductive and stabilizing agent. The generation of AgNPs was proved with UV–Vis absorbance spectra, and transmission electron microscopy (TEM) which displayed different morphologies of AgNPs relying on the heating period. The chemical formula of the polylactate sodium salt (PLS) polymer was verified with FT-IR and 1H NMR spectra. Cs-AgNPs nanohybrid was coated into an electrospun nanofibrous membrane of the cross-linked polylactate calcium salt (PLCS) to create stable polyelectrolyte complex (PEX) nanofiber-based composites with high antimicrobial activity. The produced nanohybrids were studied by X-ray diffraction (XRD), energy-dispersive X-ray analyzer (EDX), scan electron microscopy (SEM), elemental mapping and Fourier-transform infrared spectroscopy (FT-IR). AgNPs were effectively immobilized onto the PEX nanofiber-based composite, whilst there was complex generated among the electrospun PLCS and the Cs-AgNPs nanohybrid. The developed nanofibrous mats demonstrated an improved saline absorbance in the presence of AgNPs. AgNPs exhibited a homogeneous dispersion and high deposition density on the surface of nanofibers demonstrating a nanoparticle size of 7–23 nm. In addition, the silver immobilized PLCS fibers showed an improved absorbance of 0.85% (w/v) saline solution compared to blank fibers. PEX displayed high antimicrobial efficiency against gram positive and gram negative bacterial pathogens with satisfactory permeability of water vapor in the range necessary for treating wounds.

Phyto-Mediated silver nanoparticles via melissa officinalis aqueous and methanolic extracts: Synthesis, characterization and biological properties against infectious bacterial strains

Background: The present study was aimed to examine the influence of extraction method on the morphology, physico-chemical characteristics and antimicrobial properties of silver nanoparticles (AgNPs) synthesized from Melissa officinalis.Methods: AgNPs were prepared by two extraction methods. The properties of obtained nanoparticles were characterized by SEM, UV-Vis, XRD and FTIR techniques. SEM images showed different shape, size and morphology of AgNPs using two different extracts types.Results: The UV-Vis spectroscopy confirmed the formation of AgNPs by observing a distinct surface Plasmon resonance band around 450 nm. SEM images showed different shape, size and morphology of AgNPs using two different extracts types. AgNPs derived from the aqueous extract were rod-shaped with a diameter of 19 to 40 nm whereas spherical particles were synthesized by the methanolic extract found smaller with size distribution ranging from 13 to 35 nm. The XRD pattern indicated that AgNPs formed by the reduction of Ag+ ions using the methanolic extract were crystal-like in nature. The functional groups of the methanolic extract involved in synthesis and stabilization of AgNPs were investigated by FTIR. In addition, AgNPs containing methanolic extract showed higher antioxidant activity.Conclusion: The disc diffusion and agar well diffusion methods revealed the antimicrobial potential of these particles exhibited remarkable antimicrobial activity against Gram positive and Gram negative bacteria and a fungus. The production of silver nanoparticles using plant extract is rapid, low cost and eco-friendly. Silver nanoparticles can be used as an antiseptic to sterilize the surrounding area and the hospital wastes.

Anti-wear behaviour of silver nanoparticles on Al-Si alloy

In recent years, studies have shown that using nanoparticles (NPs) as lubricant additives lead to the enhancement of the anti-wear behaviour of oils. In this study, the anti-wear behaviour of silver (Ag) NPs which were capped with polyvinyl alcohol and sodium citrate were investigated for three different sliding speeds (2, 3 and 4 mm s−1) under two different loads (30 and 50 N). Results show that the AgNPs led to a decrease in friction and wear due to the formation of a tribofilm and a rolling effect. However, influence of the AgNPs declined at higher loads while increasing at higher speed test conditions. Also, both stabilising agents underwent disordering of AgNPs morphology and size successively under the applied loads.

Green synthesis of silver nanoparticles using Kalanchoe pinnata leaves (life plant) and their antibacterial and photocatalytic activities

Silver nanoparticles (AgNPs) were synthesized via green synthesis using a new herbal plant Kalanchoe pinnata (known as life plant) leaves. Effects of physicochemical parameters like temperature, pH and concentration on AgNPs were examined, and the absorption spectra were observed due to strong surface plasmon resonance. The crystalline nature and stability of AgNPs were confirmed by XRD pattern and zeta potential, respectively. The morphology of AgNPs was studied using FESEM and HRTEM. AgNPs showed antibacterial activity against the gram-negative E. Coli bacteria and photocatalytic activity in the degradation of rhodamine B dye with a reaction rate constant of 0.042 min−1.

A review on nanostructured silver as a basic ingredient in medicine: physicochemical parameters and characterization.

Recent studies with silver nanoparticles (AgNPs) and the history of silver metal as a broad-spectrum bactericidal and virucidal agent, places silver as one of the future biocidal candidates in the field of nanomedicine to eliminate bacteria and viruses, especially multidrug resistant ones. In this review, we have described the various morphologies of AgNPs and correlated the enhanced bactericidal activity with their prominent {111} facets. In addition to prioritizing the characterization we have also discussed the importance of quantifying AgNPs and silver ion content (Ag+) and their different mechanisms at the chemical, biological, pharmacological, and toxicological levels. The mechanism of action of AgNPs against various bacteria and viruses including the SARS-CoV-2 was analyzed in order to understand its effectiveness as an antimicrobial agent with therapeutic efficacy and low toxicity. Further, there is the need to characterize AgNPs and quantify the content of free Ag+ for the implementation of new systematic studies of this promising agent in nanomedicine and in clinical practice.

Moringa oleifera gum based silver and zinc oxide nanoparticles: green synthesis, characterization and their antibacterial potential against MRSA

BackgroundHerein, we first time used the gum Moringa oleifera as reducing and capping agent for successful synthesis of silver nitrate and zinc oxide nanoparticles(NPs) through green synthesis approach. This study was aimed to check antibacterial activities of synthesized NPs against multidrug resistant bacteria methicillin-resistant Staphylococcus aureus (MRSA).MethodsAqueous solutions of AgNO3 and purified gum powder were mixed with 1:1 ratio, autoclaved at 120oC for 2 min. NPs pellet collected after centrifugation at 10,000 g for 20 min. ZnO NPs were prepared by mixing purified gum powder and metal salt with1:1 ratio, heated (70oC) and stirred at 100 rpm for 4 h followed by centrifugation at 10,000 g for 20 min. Pellet was washed and calcinated at 400oC for 4 h. Antibacterial potential against E. coli, S. aureus and methicillin-resistant Staphylococcus aureus (MRSA) was assessed by widely used Kirby-Bauer antibiotic susceptibility test.ResultsOptical observation of colour change from transparent to dark and UV-Visible analysis confirmed the synthesis of NPs. Fourier transform infrared spectroscopy (FTIR) of prepared nonmaterial revealed the characteristic AgNPs and ZnO stretch vibrations at wave number of 523 cm− 1 and 471 cm− 1resectively. Crystalline nature of AgNPs and ZnO NPs was confirmed by x-ray diffraction pattern with clear sharp Peaks. Scanning electron microscopy (SEM) revealed good surface morphology of AgNPs and ZnO NPs with 50nm and 60nm size respectively. AgNPs and ZnO NPs exhibited excellent antibacterial activity against E. coli (with zone of inhibition of 21 ± 02mm and 22 ± 03mm) and S.aureus ( with zone of inhibition of 20 ± 03mm and 21 ± 02mm) while good activity was observed against “super bug” methicillin-resistant Staphylococcus aureus (MRSA) with 16 ± 03mm ad 17 ± 02mm zone if inhibitions respectively.ConclusionsThis novel addition of Moringa Gum based nanoparticles will open new dimensions in the field of nanomedicine and pharmaceutics especially against MDR bacterial strains.

Mono and Tri-cationic Imidazolium Salts: Use as Stabilizers for Silver Nanoparticles and Anticancer Study

In present strudy, the synthesis and characterization of monocationic 1,3-tetradecylimidazolium; [(C14)2Im]Br and tricationic benzene centered tris-tetradecyl/hexadecyl imidazolium bromide salts; i.e. [(C14)3C6H3Im]Br3 and [(C16)3C6H3ImBr]Br3 is reported. The stabilizer role of imidazolium salts to prepare silver nanoparticles (AgNPs) via chemical reduction method was investigated. To understand the reaction medium effect on the size and morphology control of AgNPs, monophasic (aqueous medium) and biphasic (DCM/H2O) approaches were applied. The morphology control was noticed for AgNPs protected with [(C14)3C6H3Im]Br3 (show sphere like morphology) and [(C14)2Im]Br (show dendritic structures) via biphasic approach. A clear variation in the size and morphology of AgNPs was noticed by varying the type of stabilizers and reaction medium. It was also observed that AgNPs were formed and stabilized only in aqueous medium in both approaches, thus it is assumed that AgNPs surfaces were protected by imidazolium salts with bilayer fashion. Anticancer activity of imidazolium salts was performed by MTT assay against HeLa cancer cell lines. The result shows that cytotoxic activity of tricationic [(C14)3C6H3Im]Br3 was more potent than that of monocationic [(C14)2Im]Br. The outcome suggests that there is an urgent need to develop new polycationic imidazolium salts for various chemical and medicinal applications.

Preparation, Characterization and Screening of Silver Nanoparticles Using Phenolic Rich Fractions of Amaranthus Gangeticus L. for its in-vitro Anti-Oxidant, Anti-Diabetic and Anti-Cancer Activities.

Background Nowadays green nanotechnology-based approaches using plants have been acknowledged as harmless to the ecosystem and savvy approach with different biomedical applications.Aim In the current study we prepared silver nanoparticles AgNPs in combination with phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. Leaves using a suitable eco-friendly green-synthesis way to assess its in-vitro anti-oxidative anti-diabetic and anti-cancer potential against HeLa cells.Methods Plant mediated AgNPs were synthesized using phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. and characterization was done by UVndashvisible spectroscopy X-ray diffraction XRD Fourier Transform infrared FT-IR spectroscopy Scanning Electronic Microscopy SEM and TEM analysis. With the developing use of AgNPs in biomedical viewpoints the synthesized AgNPs were assessed for its pharmacological studies.Results The synthesized AgNPs have been confirmed by change of color which was identified by UVndashvisible spectroscopy at 450 nm due to surface plasmon resonance. The crystalline property of AgNPs was identified by its XRD pattern. Using FTIR it was confirmed that the phenolic functional group present in plant extract was responsible for the reduction of silver ion and the stabilization of AgNPs. The morphology of AgNPs were studied by SEM and TEM analysis. The particles were monodispersed white and spherical ranging from 10200 nm. With the developing use of AgNPs in biomedical viewpoint the synthesized AgNPs were assessed for their in-vitro anti-oxidative anti-diabetic and anti-cancer potential against HeLa cells. The results showed that the synthesized AgNPs exhibited good anti-diabetic anti-cancer activity. It was also observed to exhibit potent anti-oxidant activity. Conclusions From the present study the synthesized AgNPs using Amaranthus gangeticus L.nbsp were found to be very potent to treat the major diseases like cancer and diabetes.

Rational Design of Ag/ZnO Hybrid Nanoparticles on Sericin/Agarose Composite Film for Enhanced Antimicrobial Applications.

Silver-based hybrid nanomaterials are receiving increasing attention as potential alternatives for traditional antimicrobial agents. Here, we proposed a simple and eco-friendly strategy to efficiently assemble zinc oxide nanoparticles (ZnO) and silver nanoparticles (AgNPs) on sericin-agarose composite film to impart superior antimicrobial activity. Based on a layer-by-layer self-assembly strategy, AgNPs and ZnO were immobilized on sericin-agarose films using the adhesion property of polydopamine. Scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray powder diffraction spectroscopy were used to show the morphology of AgNPs and ZnO on the surface of the composite film and analyze the composition and structure of AgNPs and ZnO, respectively. Water contact angle, swelling ratio, and mechanical property were determined to characterize the hydrophilicity, water absorption ability, and mechanical properties of the composite films. In addition, the antibacterial activity of the composite film was evaluated against Gram-positive and Gram-negative bacteria. The results showed that the composite film not only has desirable hydrophilicity, high water absorption ability, and favorable mechanical properties but also exhibits excellent antimicrobial activity against both Gram-positive and Gram-negative bacteria. It has shown great potential as a novel antimicrobial biomaterial for wound dressing, artificial skin, and tissue engineering.

Successful in-vivo treatment of mice infected with Candida glabrata using silver nanoparticles

The current study describes the production of silver nanoparticles (AgNPs) to treat Candida glabrata infections. The method involved incubation of silver nitrate (AgNO3) with Aspergillus terreus using a green and straightforward route. The production of AgNPs was confirmed through a color change from transparent yellow to brown as well as by ultraviolet-visible (UV-VIS) spectroscopy. The surface morphology of AgNPs was assessed using a scanning electron microscope. The antifungal activity of AgNPs against C. glabrata was investigated in the serum of 20 infected mice. The mice were divided into four groups, and the level of cytokines: IL-4 and IFN-γ were examined after 21 days. The atomic force microscopy confirmed that the average diameter of AgNPs was 25.1 nm, which is appropriate for delivering silver nanoparticles to treat animals' infection. The concentration of cytokines IL-4 and IFN-γ were significantly (P < 0.05) higher in the C. glabrata-infected group than in the control group. While the cytokines level remained close to average concentration in mice administrated with AgNPs, such a result was comparable with the fourth group of mice (Candida-treated Aspergillus) after treatment with AgNPs.

Green synthesis, characterization, antimicrobial and cytotoxic effect of silver nanoparticles using arabinoxylan isolated from Kalmegh

A green synthesis of silver nanoparticles was synthesized by AgNO3 with arabinoxylan, isolated from green stem of Andrographis paniculata (Kalmegh). The synthesized Ag NPs-arabinoxylan conjugates were characterized by UV–vis spectroscopy, FE-SEM, TEM, XRD, TGA, EDX, and Zeta potential experiments. The Ag NPs formation was established by the surface plasmon resonance band ~410.25 nm. SEM image showed mostly spherical morphology of Ag NPs. The fcc crystalline nature was identified by XRD, SAED and the size were 24.5 and 25 nm from TEM and XRD analysis respectively. The prepared Ag NPs showed dose-dependent antimicrobial activity against Streptococcus pneumonia, Candida albicans and E. coli. The nanoparciles damage 4% hemolysis to human RBCs at 12.5 μg/mL. MTT assay of Ag NPs showed that half of the cell killed at 10 μg/mL and wound healing assay observed effective inhibition cell proliferation.

Fabrication of Ag NPs/Zn-MOF Nanocomposites and Their Application as Antibacterial Agents

Three new nanocomposites consisting of Zn(II) metal–organic framework (Zn-MOF) and Ag nanoparticles (Ag NPs), designated as Ag NPs/Zn-MOFs 1–3, based on the used doses of AgNO3, were fabricated. FT-IR (Fourier-transform infrared), PXRD (powder X-ray diffraction), SEM (scanning electron microscope), EDS (energy-dispersive X-ray spectroscopy) mapping, and TEM (transmission electron microscope) techniques were used for characterization of the prepared compounds. The obtained results have shown that the Ag NPs were successfully loaded on the Zn-MOF template. The spherical morphology of Ag NPs with diameter of 30–60 nm was confirmed through TEM analysis. The antibacterial activity of the synthesized compounds was then assessed against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli, using disc diffusion method. Among the studied composites, the one with higher dose of used AgNO3, i.e. Ag NPs/Zn-MOF 1, had a broad-spectrum of antibacterial activity.

Capping action of ionic surfactants on the nucleation of lawsone-Ag+ redox system

Cetyltrimethylammonium bromide (CTAB) and sodium dodecyl sulfate (SDS) were used a stabilizing agent to determine the rate of nucleation and growth process for the reduction of Ag+ ions by lawsone. Critical micellar concentration (CMC) were calculated by using surface tension method and found to be 6.3 × 10−3 and 9.2 × 10−4 mol dm−3 for SDS and CTAB, respectively in presence of Ag+ ions. Reaction rates increases with increasing the surfactant concentrations. SDS aggregates formed complex with Ag+ ions due to electrostatic interaction with negative head group as well as solubilized the lawsone into the aqueous micellar phase through hydrophobically. CTAB effect might be due to the solubilization of lawsone into the Stern layer. The rate constants were found to be 3.2 × 10−4 and 8.1 × 10−5 s−1 for CTAB and SDS, respectively. SDS exhibited inhibition effect at post-micellar concentrations. Menger-portnoy model modified by Bunton and his coworkers was used to determine the micelles assisted parameters. For CTAB micellized reaction, binding constant (Ks = 42 mol−1 dm3 and KL = 86 mol−1 dm3), micellar phase rate constant (km = 3.5 × 10−3 s−1) were calculated with pseudo-phase kinetic model. Morphology of AgNPs strongly depends on nature of surfactant head group.

Biosynthesis of Ag nanoparticles and two-dimensional element distribution in Arabidopsis.

Metallic nanoparticles can be synthesised in living plants, which provide a friendly approach. In this work, the authors aimed to study the synthesis of silver nanoparticles (AgNPs) in Arabidopsis and the two-dimensional (2D) distribution of Ag and other elements (Ca, P, S, Mg, and CI) in the Arabidopsis plant tissues. The concentrations of Ag in the plant tissues were determined by inductively coupled plasma-atomic emission spectrometer, showing that the majority of Ag was retained in the roots. Transmission electron micrographs showed the morphology of AgNPs and the location in plant cells. The distributions of Cl and Ag were consistent in plant tissues by 2D proton-induced X-ray emission. In conclusion, this is the first report of the AgNP synthesis in Arabidopsis living plants and its 2D distribution of important elements, which provide a new clue for further research.

Fast and simple synthesis of triangular silver nanoparticles under the assistance of light

Herein, the fast and simple formation of triangular silver nanoparticles (T-Ag NPs) was systematically studied by a chemical reduction process with the assistance of light irradiation. The effects of sodium borohydride (NaBH4) concentration, pH medium, and light source irradiation are investigated via the localized surface plasmon resonances (LSPRs) detection of silver nanoparticles (Ag NPs). Results indicated that pH and light source are the two primary factors for the morphology of Ag NPs while NaBH4 plays an important role to control the formation rate of T-Ag NPs. Moreover, the photoreduction time for synthesis of T-Ag NPs is 180 min, and this method can be easily synthesized in any laboratory.

Resonant energy transfer and light scattering enhancement of plasmonic random lasers embedded with silver nanoplates.

The resonant energy transfer enhancement from a plasmonic random laser (PRL) has been investigated by means of a dye-covered PVA film with embedded silver nanoplates (DC-PVA/AgNPs). Different sizes and morphologies of AgNPs were adopted to shift the localized surface plasmon resonance (LSPR) and intensify recurrent light scattering between the AgNPs. For better overlap between surface plasmon resonance and the photoluminescence of fluorescent molecules with appropriately-sized silver nanoprisms, the slope efficiency of the PRL was greatly enhanced and the lasing threshold was obviously reduced. In addition, the photon lifetime for the DC-PVA/AgNPs film reveals an apparent decline around 1.39 ns owing to better coupling with LSPR. The stronger light scattering of samples with bigger-sized silver nanoprisms has been demonstrated by coherent back scattering measurements, which reveals a smaller transport mean free path around 3.3 μm. With α-stable analysis, it has been successfully demonstrated that the tail exponent α can be regarded as an identifier of the threshold of random lasing.

Fruit Biowaste Mediated Green Route Approach Silver Nanopartilces - As Antibacterial Material

Environmental free approach or green chemistry synthesis of metallic nanoparticles has become new growing branch of nanobiotehnology. In this present work a simple and environmental free biosynthesis silver nanoparticles (AgNPs) were prepared using Musambi Peels (MPs) aqueous extract as the reducing agent guided by the principles of green chemistry. The fruit waste aqueous extract was challenged with silver nitrate solution for the production of AgNPs in room temperature. The crystalline phase and morphology of AgNPs were determined from UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectra, X-raydiffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS).The UV-Vis spectrum indicated that the surface plasmon broad peak was observed nearby 450 nm throughout the reaction 30min-24h. XRD spectrum revealed that the average size of biowaste mediated AgNPs obtained approximately 46 nm by using the Debye-Scherrer equation. SEM image of AgNPs showed uniformly distributed on the surface of the cell with high agglomeration. EDS analysis revealed that the presence of silver was confirmed from the Ag peaks at 2.8-3.7 keV. In addition, the biowaste mediated AgNPsloaded disk were tested for antibacterial properties against Escherchia coli and Staphylococcus aureus and found that the obtained metallic AgNPs have been good antibacterial material for biological applications.