Preparation, Characterization and Screening of Silver Nanoparticles Using Phenolic Rich Fractions of Amaranthus Gangeticus L. for its in-vitro Anti-Oxidant, Anti-Diabetic and Anti-Cancer Activities.

Abstract

Background Nowadays green nanotechnology-based approaches using plants have been acknowledged as harmless to the ecosystem and savvy approach with different biomedical applications.Aim In the current study we prepared silver nanoparticles AgNPs in combination with phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. Leaves using a suitable eco-friendly green-synthesis way to assess its in-vitro anti-oxidative anti-diabetic and anti-cancer potential against HeLa cells.Methods Plant mediated AgNPs were synthesized using phenolic rich fractions of ethanolic extract of Amaranthus gangeticus L. and characterization was done by UVndashvisible spectroscopy X-ray diffraction XRD Fourier Transform infrared FT-IR spectroscopy Scanning Electronic Microscopy SEM and TEM analysis. With the developing use of AgNPs in biomedical viewpoints the synthesized AgNPs were assessed for its pharmacological studies.Results The synthesized AgNPs have been confirmed by change of color which was identified by UVndashvisible spectroscopy at 450 nm due to surface plasmon resonance. The crystalline property of AgNPs was identified by its XRD pattern. Using FTIR it was confirmed that the phenolic functional group present in plant extract was responsible for the reduction of silver ion and the stabilization of AgNPs. The morphology of AgNPs were studied by SEM and TEM analysis. The particles were monodispersed white and spherical ranging from 10200 nm. With the developing use of AgNPs in biomedical viewpoint the synthesized AgNPs were assessed for their in-vitro anti-oxidative anti-diabetic and anti-cancer potential against HeLa cells. The results showed that the synthesized AgNPs exhibited good anti-diabetic anti-cancer activity. It was also observed to exhibit potent anti-oxidant activity. Conclusions From the present study the synthesized AgNPs using Amaranthus gangeticus L.nbsp were found to be very potent to treat the major diseases like cancer and diabetes.