Abstract
Environmental free approach or green chemistry synthesis of metallic nanoparticles has become new growing branch of nanobiotehnology. In this present work a simple and environmental free biosynthesis silver nanoparticles (AgNPs) were prepared using Musambi Peels (MPs) aqueous extract as the reducing agent guided by the principles of green chemistry. The fruit waste aqueous extract was challenged with silver nitrate solution for the production of AgNPs in room temperature. The crystalline phase and morphology of AgNPs were determined from UV-Vis spectroscopy, Fourier transform infrared (FTIR) spectra, X-raydiffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS).The UV-Vis spectrum indicated that the surface plasmon broad peak was observed nearby 450 nm throughout the reaction 30min-24h. XRD spectrum revealed that the average size of biowaste mediated AgNPs obtained approximately 46 nm by using the Debye-Scherrer equation. SEM image of AgNPs showed uniformly distributed on the surface of the cell with high agglomeration. EDS analysis revealed that the presence of silver was confirmed from the Ag peaks at 2.8-3.7 keV. In addition, the biowaste mediated AgNPsloaded disk were tested for antibacterial properties against Escherchia coli and Staphylococcus aureus and found that the obtained metallic AgNPs have been good antibacterial material for biological applications.
Highlights
Nanoparticles frequently expression unique and considerably changed physical, chemical and biological properties compared to their macro scaled counterparts [1]
Musambi Peels (MPs) aqueous extract was confirmed by the UV-Vis spectrum analysis at different nm scale
The colour changed into light yellowish brown to dark brown was due to excitation of Surface Plasmon Vibration which indicated the formation of AgNPs at room temperature
Summary
Nanoparticles frequently expression unique and considerably changed physical, chemical and biological properties compared to their macro scaled counterparts [1]. Numerous noble metal nanoparticles for example copper, gold, silver and platinum were usually synthesized by using various methods including chemical, physical and biological route. The physical and chemical method nanoparticles synthesis have many disadvantages and this approach not consider as eco-friendly approach due to toxic effect that adversely effects the ecosystem. Researcher across the world has searched for novel and eco-friendly approach for the synthesis of biocompatible nanoparticles [3]. Biological route preparation of nanoparticles is less costly, less time consuming, l and more environmentally free materials, nowadays researcher are looking forward to the possible biological approach to synthesis nanoparticles [4]
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