Monosaturated Fatty Acids Research Articles

Transforming olive pits into functional foods: evaluation of phenolic, antioxidant, nutritional and microbiological properties

Background: The research aims to propose a new potential role for the olive pits as new functional foods for supporting human nutrition, health and wellbeing. The experimental research has been focused on developing a new method to suitably process the by-products of table olives (olive pits) and investigating the health-related and nutritional components of the final products.Methods: A new methodology of processing the olive pit by-product to successfully resume the edible part of the pit interior, has been developed (as showed below): In the final form of the processed olive pit, a detailed identification and determination of specific phenolic compounds with pharmacological interest was developed with a high-performance liquid chromatography- photodiode array detector (HPLC-DAD). Antioxidant activity was also evaluated with DPPH free radical scavenging assay. Other health-related nutritional parameters were also investigated, with an emphasis on fatty acid profile analysis, dietary fiber and protein concentration. Microbiological quality of the final products were also investigated. Results: Results showed that in the processed form of the olive pits, total bioactive phenolic content was found in significant levels, reaching an 8-25-fold higher concentration than the usual phenolic content of extra virgin olive oil. The quantitative determination showed that the principal biophenol determined was hydroxytyrosol, followed by tyrosol. DPPH analysis presented a high antioxidant activity, whilst the product presented considerable contents of monosaturated fatty acids, especially oleic acid, plant proteins and dietary fibers. Microbiological quality of the product was efficient in all samples tested. Conclusions: After suitable processing, the by-products of the olive pits can be considered as a valuable source of bioactive phenolic compounds with strong antioxidant activity, as well as a good source of monosaturated fatty acids, oleic acid, plant proteins and dietary fibers. Overall, the olive pits could be reconsidered as a functional food or matrix with a promising potential for pharmaceutical, nutritional and cosmetic applications.Keywords: olives; pits; phenols; antioxidants; monosaturated fatty acids; oleic acid; functional foods; by-products

Effect of Trichilia monadelpha Stem Bark Extract on the Fatty Acid Composition of Rabbit’s Thigh Meat

This study was conducted to evaluate the effects of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat. A total of 40, 6-7 weeks New Zealand white × Chinchilla crossbred rabbits with an average weight of 490 ± 4.40 were randomly divided to 4 groups with 5 replications consisting of 2 animals each in a completely randomized design. Rabbits in treatment 1 (T1) was fed basal diet with no Trichilia monadelpha stem bark extract (TMSB), T2, T3 and T4 were fed basal diet with 3mL, 6mL and 9mL TMSB per rabbit/day. Phytochemical analysis of TMSB revealed the presence of tannins (10.95 mg/g), alkaloids (9.22 mg/g), saponins (4.75 mg/g), oxalates (3.10 mg/g), flavonoids (15.88 mg/g), phenols (18.46 mg/g), terpenoids (8.62 mg/g), glycosides (7.11 mg/g) and 2-diphenyl 1-piccrlhydrazyhydrate (430.8 mg/g). Concentrations of vitamins identified in the extract are; vitamin A (2.190 mg/100g), vitamin B1 (0.567 mg/100g), vitamin B2 (0.301 mg/100g), vitamin B3 (0.227 mg/100g), vitamin B9 (0.080 mg/100g), vitamin B12 (0.209 mg/100g) and vitamin C (5.680 mg/100g). Composition of saturated fatty acid (TSFA) decrease with a significant increase in monosaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) (P˂0.05) across the treatments. It was found that TMSB significantly (P˂0.05) influenced the concentration of total unsaturated fatty acid (TSFA). Therefore, it can be concluded that TMSB is capable of modulating the fatty acid and improving the quality of meat from rabbit without causing any deleterious effect on the performance of the animal.

Insight into bioavailability of various insect meals for European perch (Perca fluviatilis): A nutritional and stable isotopic evaluation

The present study evaluated nutritional composition, stable isotopic signature, apparent digestibility coefficient (ADCs), and relative contribution of seven insect meals, namely black soldier fly (Hermetia illucens) (BSF), common housefly (Musca domestica) (CHF), yellow mealworm (Tenebrio molitor) (YMW), lesser mealworm (Alphitobius diaperinus) (LMW), house cricket (Acheta domesticus) (HC), banded cricket (Gryllodes sigillatus) (BC) and field cricket (Gryllus assimilis) (FC), on European perch (Perca fluviatilis), a potential aquaculture species in Europe.Nutritional composition on a dry matter basis varied widely across insect species. YMW, HC, and BC appeared to contain high protein content (>70%), whereas lower content was found in defatted BSF, LMW, and CHF (ranging 55–59%), adult FC remained intermediate (66.83%). Lipid levels were highest in LMW and CHF (27.72% and 26.53%, respectively), medium in BSF, HC, BC and FC, and lowest in the highly defatted YMW (8.14%). Methionine and lysine were the first limiting amino acids in all insect meals. Heatmap visualization indicated considerable similarity in amino acid profile between fishmeal and YMW. Most insect meals contain a high amount of saturated, monosaturated fatty acids and a lack of n-3 fatty acids. Although macro-minerals were lower in insect meals than in fishmeal, the trace minerals of YMW, BSF, HC, and FC surpassed fishmeal, implying that insect meals are an excellent source of trace minerals.The digestibility of nutrients and energy differed significantly among insect meals. Dry matter ADC varied from 67.91% (YMW) to 85.02% (LMW), with LMW and CHF significantly higher than YMW. Protein ADC was lowest in YMW (69.03%) and greatest in LMW (91.94%) and CHF (93.53%). BSF and HC exerted absolute lipid digestibility (104.86 and 99.99%, respectively), followed by LMW (94.75%), whereas BC had the lowest lipid digestibility among insect meals. Energy digestibility favored BSF, CHF, FC, HC, and LMW, but not BC and YMW. Ash and phosphorus digestibility did not differ among insect meals.FC contributed significantly to perch muscle development (mean and interquartile range; 23.9, 10.6–33.8%), followed by CHF (19.8%, 8.8–29.7%), whereas BC imprints relatively low contribution (12.6%, 7.4–17.4%). The stable isotope and mixing models provide insight into the role of ingredients in the accretion of perch muscle as well as in nutritional complementarity among them in satisfying nutrient requirements, thus growth performance of perch.

The Impact of Insect Flour on Sourdough Fermentation-Fatty Acids, Amino-Acids, Minerals and Volatile Profile.

Simple SummaryAcheta domesticus (house cricket) flour is one of the most promising and sustainable sources of nutrients. It is rich in protein, minerals, fatty acids, amino acids, and vitamins and it has a low impact on the consumption of natural environmental resources. On the other side, fermentation with lactic acid bacteria represents a technological tool that can improve further the nutritional quality of flours. Therefore, the aim of the present research was to study the adaptability of the Lactobacillus plantarum strain on insect flour fermentation. Fatty acids, amino acids, minerals, and aroma volatile compounds were analyzed during 48 h of fermentation. Fermentation improved the nutritional quantity of the bioactive compounds, mainly after 24 h of fermentation, where they reached higher extended values. Overall, our findings indicate that insect flour is able to support the growth and development of the Lactobacillus plantarum strain, leading to an enriched insect flour sourdough that could be further used in the manufacturing of new products.Acheta domesticus (L.1758) has been recently accepted by the European Union as a novel food, being the third insect that has been approved for human consumption. Nowadays, researchers’ attention is focused on exploiting new protein sustainable sources, and, therefore, insect flour has gained more and more interest. Organic acids, fatty acids, amino acids, aroma volatile compounds, and minerals were analyzed through HPLC-RID (High-performance liquid chromatography), GC-MS (Gas chromatography-mass spectrometry), LC-MS (Liquid chromatography–mass spectrometry), ITEX/GC-MS and AAS (Atomic Absorption Spectrophotometry), respectively. Fermentation of the insect flour with Lactobacillus plantarum ATCC 8014 strain (Lp) leads to an increase in organic acids such as lactic, acetic, and oxalic, whilst citric acid decreases its value. SFA (saturated fatty acids) and MUFA (monosaturated fatty acids) groups were positively influenced by Lp fermentation; meanwhile, PUFA (polysaturated fatty acids) decreased during fermentation. A positive trend was observed for amino acids, aroma volatile content, and minerals enhancement during insect sourdough fermentation, mainly at 24 h of fermentation. Acheta domesticus (A. domesticus) sourdough fermentation represents a new tool that needs to be further exploited aiming to improve the nutritional qualities of the final products.

Fatty Acid Profile and Antioxidant Capacity of Dabai (Canarium odontophyllum L.): Effect of Origin and Fruit Component.

In the present work, the influence of geographical location on the fatty acid profiles, antioxidant potential, as well as cytotoxicity of edible dabai fruit fractions (kernel, skin, and pulp) were analyzed. The fatty acid profiles were determined by Gas Chromatography (GC), and the antioxidant activity was quantified with free 2,2-diphenyl-1-picr/ylhdrazyl, while the cytotoxicity was assessed by the brine shrimp lethality test. The results showed that the samples from Sibu, Serian, and Kapit geographical locations had a high content of the saturated fatty acids, ranging from 46.63% to 53.31% in the three fractions. The highest mono-saturated fatty acids (MUFA) content was found in Sibu. Serian and Kapit kernel fractions MUFA, however, ranged from 21.2% to 45.91%. No fatty acid composition was detected in Bentong and Kanowit. The fatty acid composition and DPPH free radical scavenging antioxidant activity of dabai were statistically independent using a multivariate analysis in different localities in Malaysia. The skin fraction had a more appreciable antioxidant potential and toxicity level than the pulp and kernel fractions. The highest antioxidant activity (EC50 198.76 ± 1.06 µg/mL) with an LC50 value of 1387.22 µg/mL was obtained from the Sibu skin fraction. Therefore, the fatty acid composition, antioxidant, as well as cytotoxicity analyses of the extracts from different localities indicated that “geographical location” remarkably influenced fatty acid composition, antioxidant activity, and toxicity.

Evaluation of a Whole-Liver Dixon-Based MRI Approach for Quantification of Liver Fat in Patients with Type 2 Diabetes Treated with Two Isocaloric Different Diets.

Dixon-based methods for the detection of fatty liver have the advantage of being non-invasive, easy to perform and analyze, and to provide a whole-liver coverage during the acquisition. The aim of the study was to assess the feasibility of a whole-liver Dixon-based approach for liver fat quantification in type 2 diabetes (T2D) patients who underwent two different isocaloric dietary treatments: a diet rich in monosaturated fatty acids (MUFA) and a multifactorial diet. Thirty-nine T2D patients were randomly assigned to MUFA diet (n = 21) and multifactorial diet (n = 18). The mean values of the proton density fat fraction (PDFF) over the whole liver and over the ROI corresponding to that chosen for MRS were compared to MRS-PDFF using Spearman’s correlation (ρ). Before–after changes in percentage of liver volume corresponding to MRI-PDFF above thresholds associated with hepatic steatosis (LV%TH, with TH = 5.56%, 7.97% and 8.8%) were considered to assess the proposed approach and compared between diets using Wilcoxon rank-sum test. Statistical significance set at p < 0.05. A strong linear relationship was found between MRS-PDFF and MRI-PDFFs (ρ = 0.85, p < 0.0001). Changes in LV%TH% were significantly higher (p < 0.05) in the multifactorial diet than in MUFA diet (25% vs. 9%, 35% vs. 12%, and 38% vs. 13% decrease, respectively, for TH = 5.56%, 7.97%, and 8.8%) and this was reproducible compared to results obtained using the standard liver fat analysis. A volumetric approach based on Dixon method could be an effective, non-invasive technique that could be used for the quantitative analysis of hepatic steatosis in T2D patients.

Bioaccessibility and Oxidative Stability of Omega-3 Fatty Acids in Supplements, Sardines and Enriched Eggs Studied Using a Static In Vitro Gastrointestinal Model.

Modern dietary habits have created the need for the design and production of functional foods enriched in bioactive compounds for a healthy lifestyle. However, the fate of many of these bioactive compounds in the human gastrointestinal (GI) tract has not been thoroughly investigated. Thus, in the present study, the bioaccessibility of omega-3 fatty acids was examined. To that end, different foods and supplements underwent simulated digestion following the INFOGEST protocol. The selected samples were foods rich in omega-3 fatty acids both in free and bound form—i.e., dietary fish oil supplements, heat-treated fish, and eggs enriched with omega-3 fatty acids. The oxidation of polyunsaturated fatty acids (PUFAs) was measured at each stage of the digestion process using peroxide value (PV) and TBARS and by quantifying individual omega-3 fatty acids using a gas chromatograph with flame ionization detector (GC-FID). The final bioaccessibility values of omega-3 fatty acids were determined. Changes in the quantity of mono-saturated fatty acids (MUFAs) and saturated fatty acids (SFAs) were recorded as well. The results indicated a profound oxidation of omega-3 fatty acids, giving rise to both primary and secondary oxidation products. Additionally, stomach conditions seemed to exert the most significant effect on the oxidation of PUFAs during digestion, significantly decreasing their bioaccessibility. The oxidation rate of each fatty acid was found to be strongly correlated with its initial concentration. Finally, the oxidation pattern was found to be different for each matrix and emulsified lipids seemed to be better protected than non-emulsified lipids. It is concluded that digestion has a profound negative effect on omega-3 bioaccessibility and therefore there is a need for improved protective mechanisms.

Exploration of Ti6Al4V surface grinding under dry and MQL environments

Nowadays the use of sustainable/environment friendly methods are rising in surface grinding process. In this work, the surface grinding of Ti-6Al-4V alloy with cubic boron nitride (CBN) grinding wheel under dry and minimum quantity lubrication (MQL) environment was performed. Experiments under MQL condition enhanced the overall grinding performance in terms of surface roughness and tangential cutting forces. Efficient lubrication under MQL environment due to presence of high anti-oxidation mono-saturated fatty acid percentage and lesser poly-saturated fatty acid percentage (easily oxidized) provides excellent lubrication and cooling action at the grinding zone in comparison to dry condition. Under dry grinding as the number of grinding passes increased from 2nd to 8th pass surface roughness increased by 166%. At higher grinding pass (8th) surface roughness decreased by 24% in contrast to 1st pass. Further, cutting force under dry grinding case with 8th pass got increased by 18% in contrast to MQL condition.

Extracellular lipidome change by an SGLT2 inhibitor, luseogliflozin, contributes to prevent skeletal muscle atrophy in db/db mice.

BackgroundDiabetes mellitus increases the excretion of urinary glucose from the renal glomeruli due to elevated blood glucose levels. In the renal tubules, SGLT2 is expressed and reabsorbs the excreted urinary glucose. In the pathogenesis of diabetes mellitus, glucose reabsorption by SGLT2 is increased, and SGLT2 inhibitors improve hyperglycaemia by inhibiting this reabsorption. When urinary glucose excretion is enhanced, glucose supply to skeletal muscle may be insufficient and muscle protein catabolism may be accelerated. On the other hand, SGLT2 inhibitors not only ameliorate hyperglycaemia but also improve fatty acid metabolism in muscle, which may prevent muscle atrophy.MethodsEight‐week‐old male db/m mice or db/db mice were fed a standard diet with or without the SGLT2i luseogliflozin (0.01% w/w in chow) for 8 weeks. Mice were sacrificed at 16 weeks of age, and skeletal muscle and serum lipidomes, as well as skeletal muscle transcriptome, were analysed.ResultsAdministration of SGLT2i led to not only decreased visceral fat accumulation (P = 0.004) but also increased soleus muscle weight (P = 0.010) and grip strength (P = 0.0001). The levels of saturated fatty acids, especially palmitic acid, decreased in both muscles (P = 0.017) and sera (P = 0.041) upon administration of SGLT2i, while the content of monosaturated fatty acids, especially oleic acid, increased in both muscle (P < 0.0001) and sera (P = 0.009). Finally, the accumulation of transcripts associated with fatty acid metabolism, such as Scd1, Fasn, and Elovl6, and of muscle atrophy‐associated transcripts, such as Foxo1, Mstn, Trim63, and Fbxo32, decreased following SGLT2i administration.ConclusionsIntramuscular fatty acid metabolism and gene expression were influenced by the extracellular lipidome, which was modified by SGLT2i. Hence, secondary effects, other than the hypoglycaemic effects of SGLT2i, might lead to the alleviation of sarcopenia.

Hepatic transcriptome analysis reveals altered lipid metabolism and consequent health indices in chicken supplemented with dietary Bifidobacterium bifidum and mannan-oligosaccharides

This study investigated the role of dietary prebiotic mannan-oligosaccharides (MOS), and probiotic Bifidobacterium bifidum (BFD) in lipid metabolism, deposition, and consequent health indices in broiler chicken. The supplementation of 0.2% MOS along with either 106 or 107 CFU BFD/g feed resulted in downregulation of Acetyl-CoA carboxylase, fatty acid synthase, sterolregulatory element binding protein-1, and apolipoprotein B100; and up-regulation of peroxisome proliferator activated receptor-α AMP-activated protein kinase α-1, and stearoyl CoA (∆9) desaturase-1 hepatic expression in broiler chicken. The birds supplemented with 0.2% MOS along with either 106 or 107 CFU BFD/g feed depicted lower body fat percentage, palmitic acid, stearic acid, and saturated fatty acid contents, whereas, higher palmitoleic acid, oleic acid, and MUFA contents were observed. The ∆9-desaturase indices of chicken meat have shown higher values; and elongase index (only thigh) and thioesterase index have shown lower values in birds supplemented with 0.2% MOS along with either 106 or 107 CFU BFD/g feed. The meat health indices such as Polyunsaturated fatty acids (PUFA)/Saturated fatty acids (SFA) ratio, Mono-saturated fatty acids (MUFA)/SFA ratio, unsaturated fatty acids (UFA)/SFA ratio, hypocholesterolemic/hypercholesterolemic fatty acid ratio, saturation index, atherogenic index, thrombogenic index, and hypercholesterolemic fatty acid content were positively improved in birds supplemented with 0.2% MOS along with either 106 or 107 CFU BFD/g feed. Similarly, the birds supplemented with 0.2% MOS along with either 106 or 107 CFU BFD/g feed have shown lower serum triglyceride and total cholesterol levels along with higher high density levels and improved serum health indices cardiac risk ratio, atherogenic coefficient, and, atherogenic index of plasma.

Proximate compositions, fatty acids and mineral contents of chicken and turkey gizzards, and textural properties after tenderization with proteolytic enzymes

The purpose of this study was to determine the proximate compositions, mineral contents and fatty acid compositions of chicken and turkey gizzards. In addition, the textural properties of the gizzards were determined after tenderization with sous vide cooking and proteolytic enzymes, such as bromelain, ficin, and papain. Four different groups (control, bromelain, ficin, and papain) for each gizzard species (chicken and turkey) were prepared for the tenderization part of this study. Firmness, work of shear, and collagen content of tenderized gizzards were determined. Results revealed that gizzards were a good source in terms of macro and trace elements, such as Zn and Fe. Chicken and turkey gizzards had low total saturated fatty acid content. Total monosaturated fatty acid content was found as 37.38% for chicken gizzard and 44.82% for turkey gizzard. Proteolytic enzyme treatments increased the tenderness of gizzards. Practical applications Gizzard is one of the edible by-products in the poultry industry. It is an economical source of protein and other nutrients since it is cheaper compared to chicken and turkey meat. In this study, it was revealed that gizzard is a good source in terms of protein, macroelements, and trace elements, such as iron and zinc. Additionally, consumption of gizzard is thought to be beneficial for human health since gizzards contain lower total saturated fatty acid levels and higher total monosaturated fatty acid. However, gizzards have tough texture due to their high content of collagen. For this reason, in this study, chicken and turkey gizzards were tenderized with plant-derived proteolytic enzymes and sous vide cooking.

Efficacy of single and mix probiotic bacteria strain on growth indices, physiological condition and bio-chemical composition of juvenile amberjack (Seriola dumerili)

This study presents the effect of integrating single strain (Bacillus amyloliquefaciens (BA)) and mix strain (Streptococcus faecalis (SF), Lactobacillus plantarum (LP) and Bacillus mesentericus (BM)) probiotic bacteria on growth, physiologycal condition and body composition of juvenile Amberjack (initial average body weight 5.61 ± 0.11 g). Three experimental diets were formulated, the basal diet CD1 (0% probiotic supplement), diet D2 (0.2 % BA supplement, 1.59 × 108 CFU/g) and diet D3 (1% SF + LP + BM supplement, 2.86 × 108 CFU/g diet). Fifty fish were stock in each 500 L poly tank (duplicate tank per treatment) and fed the test diets for 75 days. Sampling of weights and length was conducted at 60 and 75 days, while the whole body samples were collected at the end of the feeding trial. The results showed a significantly higher condition factor (K), relatively condition factor (Kn), and a numerically high length weight relationship (LWRs) coefficient (b) for fish feed BA supplement compared to control and SF + LP + BM group. The coefficient (b) values of the allometric growth trend was improved for BA supplement with no significant difference from isometric growth coefficient (b = 3). The growth parameters, feed utilization, survival (%) and whole body composition of fish fed the test diets were not significantly altered. A numerically high mono-saturated fatty acid (MUFA) and, Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) ratio was noted in fish group fed BA supplement, suggesting an improve physiological condition of fish. This results demonstrated that integration of single strain Bacillus amyloliquefaciens at 0.2 % has significantly improved physiological condition for Amberjack juvenile. Further research is recommended to determine the dietary effects of the single strain (BA) and mix strain (SF + BM + LP) on growth and health of fish.

Influence of the Different Carbon and Nitrogen Sources on the Production of Biodiesel by Oleaginous Fungi Aspergillus terreus, Aspergillus fumigatus

In the present study, the growth and total lipid contents of two oleaginous fungal isolates Aspergillus terreus, Aspergillus fumigatus were compared in different nitrogen and organic carbon sources. Artificially the fungi were cultured on media consisting of various mono- or di- or polysaccharides and peptone or yeast extract as elementary sources for carbon and nitrogen, respectively. Media containing sucrose /yeast extract or glucose/ yeast extract were the most effective for lipid production from fungal, during two weeks incubation period, the highest biomass of dry weight was (19.6 , 18.8) g / L , (25.8 , 30.5) g /L and lipid yield (1, 0.97 )g/L, (0.65, 0.65) g/ L for two isolates Aspergillus terreus and Aspergillus fumigatus respectively then followed maltose/ yeast extract then starch / yeast extract .Analysis of fatty acid produced by two fungal isolates using Gas Chromatography showed the existence of a wide range of fatty acids, these fatty acids were together saturated and unsaturated. The results revealed that the unevenness in the fatty acids composition mainly depends on the type of carbon and nitrogen sources. The existence of saturated and mono saturated fatty acids in A.fumigatus, A. terreus prove that they are good candidate for biodiesel production. The analysis of fatty acid from the fungal growth showed the existence of a wide range of fatty acids Palmitic ,Oleic acids , stearic acid and linoleic acid together saturated and unsaturated. The results revealed that the unevenness in the fatty acid composition mainly depends on the type of carbon and nitrogen sources. The presence of saturated and mono saturated fatty acid in A.fumigatus , A. terreus prove that it is a good candidate for biodiesel production.

Cultivation of Tetraselmis suecica using a fishmeal factory effluent: effect on the growth, biochemical composition, and nutrient removal

One of the main effluents from fishmeal plants is stickwater (STW) that causes a considerable number of environmental hazards. This study aimed to replace standard growth medium (F/2; as an enriched solution for growing most of the marine microalgae) of Tetraselmis suecica with different ratios of STW from a kilka fishmeal plant including 0% (control), 10%, 25%, 50%, 75%, and 100%. Results showed that the highest nutrient removal efficacy was obtained in 75% STW treatment, followed by the highest algal cell density (1.23 × 107 cells mL−1) (P <0.05). A significant increase in the concentrations of photosynthetic pigments (chlorophyll a, b) and lipid productivity was recorded in 75% STW treatment compared to others (P <0.05). The maximum contents of carotenoids and crude protein were obtained in 100% and 50% STW groups, respectively. The levels of saturated and monosaturated fatty acids were significantly increased by increasing the inclusion ratio of STW from 50 to 100% (P <0.05). However, the lowest level of polyunsaturated fatty acids was measured in 100% STW treatment. Overall, replacing F/2 with STW up to 75% is feasible to culture T. suecica with the highest biomass and lipid productivity.

Sifat Fisika Kimia Campuran Minyak dari Minyak Sawit Merah Murni dengan Minyak Zaitun, Minyak Jagung, Minyak Kedelai atau Minyak Bunga Matahari

Minyak sawit merah murni (virgin red palm oil, VRPO) merupakan minyak mengandung asam lemak jenuh dan tak jenuh seimbang, serta senyawa bioaktif tinggi (seperti karoten, tokoferol dan tokotrienol). Sementara itu, minyak zaitun (olive oil, OO), minyak jagung (corn oil, CO), minyak kedelai (soybean oil, SBO) dan minyak bunga matahari (sunflower oil, SFO) merupakan minyak mengandung asam lemak tak jenuh tinggi. Pencampuran dua atau lebih jenis minyak dapat menghasilkan minyak sehat dengan profil asam lemak, stabilitas oksidatif dan senyawa bioaktif yang diinginkan. Penelitian ini dilakukan untuk mengkaji karakteristik campuran VRPO dengan OO, CO, SBO atau SFO pada rasio berat 100:0-0:100 meliputi kadar asam lemak bebas, karoten dan vitamin E, komposisi asam lemak, bilangan iodin, titik leleh, dan kandungan lemak padat. Peningkatan jumlah VRPO meningkatkan kadar asam palmitat, karoten dan vitamin e, titik leleh dan kandungan lemak padat. Peningkatan jumlah OO, CO, SBO atau SFO menurunkan kadar asam lemak bebas dan meningkatkan bilangan iodin. Campuran VRPO dengan CO, SBO atau SFO menghasilkan minyak sehat dengan rasio asam lemak jenuh: asam lemak tak jenuh tunggal: asam lemak tak jenuh ganda mendekati 1:1,5:1,0. Campuran VRPO dengan OO juga menghasilkan minyak sehat dengan rasio asam linoleat: asam linolenat sebesar 5-10:1. Campuran-campuran minyak tersebut dapat digunakan sebagai bahan baku untuk minyak goreng, baking shortening dan margarin yang kaya senyawa bioaktif.

TILLING-by-Sequencing+ to Decipher Oil Biosynthesis Pathway in Soybeans: A New and Effective Platform for High-Throughput Gene Functional Analysis.

Reverse genetic approaches have been widely applied to study gene function in crop species; however, these techniques, including gel-based TILLING, present low efficiency to characterize genes in soybeans due to genome complexity, gene duplication, and the presence of multiple gene family members that share high homology in their DNA sequence. Chemical mutagenesis emerges as a genetically modified-free strategy to produce large-scale soybean mutants for economically important traits improvement. The current study uses an optimized high-throughput TILLING by target capture sequencing technology, or TILLING-by-Sequencing+ (TbyS+), coupled with universal bioinformatic tools to identify population-wide mutations in soybeans. Four ethyl methanesulfonate mutagenized populations (4032 mutant families) have been screened for the presence of induced mutations in targeted genes. The mutation types and effects have been characterized for a total of 138 soybean genes involved in soybean seed composition, disease resistance, and many other quality traits. To test the efficiency of TbyS+ in complex genomes, we used soybeans as a model with a focus on three desaturase gene families, GmSACPD, GmFAD2, and GmFAD3, that are involved in the soybean fatty acid biosynthesis pathway. We successfully isolated mutants from all the six gene family members. Unsurprisingly, most of the characterized mutants showed significant changes either in their stearic, oleic, or linolenic acids. By using TbyS+, we discovered novel sources of soybean oil traits, including high saturated and monosaturated fatty acids in addition to low polyunsaturated fatty acid contents. This technology provides an unprecedented platform for highly effective screening of polyploid mutant populations and functional gene analysis. The obtained soybean mutants from this study can be used in subsequent soybean breeding programs for improved oil composition traits.

BIMG-10. IDH1 MUTATIONS INDUCE ORGANELLE DEFECTS VIA DYSREGULATED PHOSPHOLIPIDS

BACKGROUNDMetabolic alterations of lipids have been identified as a hallmark of neoplasms, with the most prevalent being the balance between saturated fatty acid (SFA) and monosaturated fatty acid (MUFA). Stearoyl-CoA desaturase1 (SCD1), converting SFA to MUFA, is increased in many cancers, leading to worse prognosis. In glioma, the role of SCD1 remains unknown. Isocitrate dehydrogenase (IDH) mutations have been most commonly observed in glioma, but the involvement of mutant IDH in SCD1 expression also remains unknown.METHODSWe conducted metabolic analysis to examine the alteration of SCD1 expression in genetically engineered glioma cell lines and normal human astrocyte (NHA). Lipid metabolic analysis was conducted by using LC-MS, Raman Imaging Microscopy and SCD1 expression was examined by Western-blotting and RT-PCR method. Electron microscopy was employed for organelle structure and genetic knock-down of SCD1 gene was performed.RESULTHerein, we uncovered increased MUFA and their phospholipids in Endoplasmic Reticulum (ER), generated by IDH1 mutation, that were responsible for Golgi and ER dilation. RNA seq data from The Cancer Genome Atlas, showed that SCD1 expression was significantly higher in IDH mutant gliomas compared with wild-type, and high SCD1 expression was associated with longer survival. Inhibition of IDH1 mutation or SCD1 silencing restored ER and Golgi morphology, while D-2HG and oleic acid induced morphological defects in these organelles. Moreover, addition of oleic acid, which tilts the balance towards elevated levels of MUFA, produced IDH1 mutant-specific cellular apoptosis.CONCLUSIONCollectively, our results suggest that IDH1 mutant-induced SCD overexpression can rearrange the distribution of lipids in the organelles of glioma cells, providing a new insight on the link between lipids metabolism and organelle morphology in these cells, with potential and unique therapeutic implications. The results of the present study may also provide novel insights into the discovery of metabolic biomarkers for IDH mutant gliomas.

Insights into the physiology of Chlorella vulgaris cultivated in sweet sorghum bagasse hydrolysate for sustainable algal biomass and lipid production

Supplementing cultivation media with exogenous carbon sources enhances biomass and lipid production in microalgae. Utilization of renewable organic carbon from agricultural residues can potentially reduce the cost of algae cultivation, while enhancing sustainability. In the present investigation a medium was developed from sweet sorghum bagasse for cultivation of Chlorella under mixotrophic conditions. Using response surface methodology, the optimal values of critical process parameters were determined, namely inoculum cell density (O.D.750) of 0.786, SSB hydrolysate content of the medium 25% v/v, and zero medium salinity, to achieve maximum lipid productivity of 120 mg/L/d. Enhanced biomass (3.44 g/L) and lipid content (40% of dry cell weight) were observed when the alga was cultivated in SSB hydrolysate under mixotrophic conditions compared to heterotrophic and photoautotrophic conditions. A time course investigation revealed distinct physiological responses in terms of cellular growth and biochemical composition of C. vulgaris cultivated in the various trophic modes. The determined carbohydrate and lipid profiles indicate that sugar addition to the cultivation medium boosts neutral lipid synthesis compared to structural lipids, suggesting that carbon flux is channeled towards triacylglycerol synthesis in the cells. Furthermore, the fatty acid profile of lipids extracted from mixotrophically grown cultures contained more saturated and monosaturated fatty acids, which are suitable for biofuel manufacturing. Scale-up studies in a photobioreactor using SSB hydrolysate achieved a biomass concentration of 2.83 g/L consisting of 34% lipids and 26% carbohydrates. These results confirmed that SSB hydrolysate is a promising feedstock for mixotrophic cultivation of Chlorella and synthesis of algal bioproducts and biofuels.

Blended Sewage Sludge–Palm Kernel Expeller to Enhance the Palatability of Black Soldier Fly Larvae for Biodiesel Production

Black soldier fly larvae (BSFL) have been employed for valorizing organic waste materials as the larvae are able to consume organic waste and transform it into valuable larval biomass. In this study, BSFL were found to potentially reduce blended sewage sludge. The addition of palm kernel expeller (PKE) fortified the protein and lipid content in blended sewage sludge substrates, leading to larval growth enhancement. In addition, the larval weight also influenced the lipid yield and fatty acid methyl ester (FAME) profile. However, the optimum ratio of sewage sludge to PKE had to be determined as excess PKE content could become a threat to larval growth by contributing to the reduction of non-fiber carbohydrates content in the feed, thereby resulting in the decrease in lipid yield and FAME content. In this work, a sewage sludge to PKE ratio of 2:3 proffered the highest larval weight gained at 46.99 ± 2.09 mg/larva. Meanwhile, a proportion of 3:2 of sewage sludge to PKE was able provide the highest lipid yield of 17 ± 1.77%. Furthermore, the FAME profile revealed the presence of a significant amount of saturated and monosaturated fatty acids, indicating a good quality biodiesel. Thus, BSFL-based biodiesel fed with blended sewage sludge and PKE could be utilized for producing a high quality biodiesel. However, further improvement on the amount of lipid yield and FAME content should be further investigated.

Homogeneous catalyzed transesterification of neem seed oil to biodiesel and its kinetic modeling

AbstractThis study focuses on the kinetic modeling of homogeneous catalyzed transesterification of neem seed oil and the determination of the rate‐limiting step (RLS). The oil was extracted from the seeds by cold solvent extraction and it was characterized to determine its physicochemical properties. The effect of factors such as time, amount of catalyst, temperature, methanol / oil molar ratio, and agitation speed on the biodiesel yield were investigated. The kinetic experiments for the transesterification reaction were carried out by batch process, assuming a pseudo second‐order reaction. Rate equations for the arrays of reversible reactions of triglyceride to glycerol and biodiesel were developed to determine the RLS. The oil yield extracted was 34%. The extracted oil contained 60.2% monounsaturated fatty acid and the dominant monosaturated fatty acid is oleic acid having 40.6% of total composition of monosaturated fatty acid in the oil. The biodiesel yield produced by transesterification of pretreated neem oil was 90% and biodiesel properties were within the limits of American Society for Testing and Materials (ASTM) (D6751‐02) standards. The kinetic modeling of the reaction revealed that the rate constant values for forward reactions (transesterification) (k1, k3, and k5) were higher than those of backward reactions (k2, k4 and k6) at temperatures of 45, 55, and 65 °C. The rate constant (k1) for the conversion of triglyceride to diglyceride was the least among the rate constants of forward reactions at all the studied temperatures showing the RLS. The rate constants for the RLS are 0.0174, 0.0208, and 0.0303 min−1 at temperatures of 45, 55, and 65 °C respectively and the activation energy is 24.9 kJ mol−1. © 2020 Society of Industrial Chemistry and John Wiley &amp; Sons Ltd