Monosaturated Fatty Acids Research Articles

Improving egg quality in laying hens using calcium salt of black soldier fly larvae oil

Abstract Black soldier fly larvae (BSFL) oil has gained attention as a potential substitute for vegetable oil in feed formulations designed to improve productivity and product quality. This study aimed to investigate the impact of supplementation BSFL oil calcium salt (BSFLO-SCa) on the egg quality and gene expression involved in fat metabolism in laying hens. A total of sixty 40-wk-old ISA Brown laying hens were divided into three distinct groups: a control group (T0) that received a basic diet, and two treatment groups that were supplemented with 1% (T1) and 2% (T2) BSFLO-SCa. Each group consisted of 5 replicates, with 4 laying hens per replicate. The study indicated that the inclusion of 2% of BSFLO-SCa significantly increased egg albumen weight, height, ratio, eggshell weight, and thickness (). The inclusion of 1 and 2% of BSFLO-SCa increased the yolk lightness (L*) and yellowness (b*), but redness (a*) was decreased (). Moreover, BSFLO-SCa supplementation with 1 and 2% reduced () egg fat and cholesterol. Analysis of fatty acid profiles showed significantly increased medium-chain fatty acid (MCFA) and polyunsaturated fatty acid (PUFA) (), while monosaturated fatty acid (MUFA) reduced () with 2% BSFLO-SCa. Gene expression related to lipid metabolism, such as fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC), as well as cholesterol synthesis gene expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were downregulated (). In conclusion, the inclusion of 2% BSFLO-SCa in the diet improved quality and reduced fat and cholesterol content in eggs from laying hens. This improvement was accompanied by downregulation of key genes involved in lipid metabolism.

Upstream and downstream pathways of diacylglycerol kinase : Novel phosphatidylinositol turnover-independent signal transduction pathways

Diacylglycerol kinase (DGK) phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). Mammalian DGK comprise ten isozymes (α–κ) that regulate a wide variety of physiological and pathological events. Recently, we revealed that DGK isozymes use saturated fatty acid (SFA)/monosaturated fatty acid (MUFA)-containing and docosahexaenoic acid (22:6)-containing DG species, but not phosphatidylinositol (PI) turnover-derived 18:0/20:4-DG. For example, DGKδ, which is involved in the pathogenesis of type 2 diabetes, preferentially uses SFA/MUFA-containing DG species, such as 16:0/16:0- and 16:0/18:1-DG species, in high glucose-stimulated skeletal muscle cells. Moreover, DGKδ, which destabilizes the serotonin transporter (SERT) and regulates the serotonergic system in the brain, primarily generates 18:0/22:6-PA. Furthermore, 16:0/16:0-PA is produced by DGKζ in Neuro-2a cells during neuronal differentiation. We searched for SFA/MUFA-PA- and 18:0/22:6-PA-selective binding proteins (candidate downstream targets of DGKδ) and found that SFA/MUFA-PA binds to and activates the creatine kinase muscle type, an energy-metabolizing enzyme, and that 18:0/22:6-PA interacts with and activates Praja-1, an E3 ubiquitin ligase acting on SERT, and synaptojanin-1, a key player in the synaptic vesicle cycle. Next, we searched for SFA/MUFA-DG-generating enzymes upstream of DGKδ. We found that sphingomyelin synthase (SMS)1, SMS2, and SMS-related protein (SMSr) commonly act as phosphatidylcholine (PC)-phospholipase C (PLC) and phosphatidylethanolamine (PE)-PLC, generating SFA/MUFA-DG species, in addition to SMS and ceramide phosphoethanolamine synthase. Moreover, the orphan phosphatase PHOSPHO1 showed PC- and PE-PLC activities that produced SFA/MUFA-DG. Although PC- and PE-PLC activities were first described 70–35 years ago, their proteins and genes were not identified for a long time. We found that DGKδ interacts with SMSr and PHOSPHO1, and that DGKζ binds to SMS1 and SMSr. Taken together, these results strongly suggest that there are previously unrecognized signal transduction pathways that include DGK isozymes and generate and utilize SFA/MUFA-DG/PA or 18:0/22:6-DG/PA but not PI-turnover-derived 18:0/20:4-DG/PA.

140 Effects of ɷ-3 PUFA supplementation during late gestation to first-calf beef heifers on physiological and performance responses of offspring

Abstract The objectives of this experiment were to evaluate the effects of ɷ-3 PUFA supplementation to late gestation first-calf beef heifers on milk and immunoglobulin production and performance and physiological responses of the offspring. Non-lactating, pregnant Angus heifers (n = 44) were ranked by age, body weight (BW), and body condition score (BCS) and allocated to receive DDG + 1 of the 2 treatments: 1) 234 g/heifer per feeding of Ca salts of PUFA based on eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids (OMG, Strata; Virtus Nutrition LLC, Corcoran, CA; n = 22), 2) 234 g/heifer per feeding of Ca salts of saturated and monosaturated fatty acids based on palmitic and oleic acids (CON; EnerGII, Virtus Nutrition, LLC; n = 22; Table 1). The animals were gathered each week on Mondays, Wednesdays, and Fridays and individually fed DDG + treatments. Immediately after calving, heifer-calf pairs were removed from their respective treatments and assigned to the general management of the research herd until weaning, which did not include fatty acid supplementation. Calves were transported to a feedyard for a 90-d preconditioning and feedlot receiving period. Initial cow BW and BCS were similar (P ≥ 0.49) among treatments. No differences were also detected (P ≥ 0.33) for BW or BCS change, and at calving BW or BCS (Table 3). No differences were observed between groups for average colostrum composition (P > 0.37). However, there was an increase in colostrum fat percentage (P = 0.03) and a tendency of greater concentration of colostrum IgG (P = 0.08) in OMG heifers compared with the control group. No significant differences were observed among treatments at calving results (P > 0.83; Table 3). However, a tendency was observed for crown to rump measure (P = 0.07). During the preconditioning period, there was a difference among groups for final BW (P = 0.04) and average daily gain (ADG; P = 0.05), remaining throughout the receiving phase among treatments for initial BW (P = 0.04), final BW (P = 0.04) and ADG (P = 0.05). A tendency (P = 0.09) for greater NEFA concentration and greater cortisol concentrations (P = 0.05) was observed in OMG vs. CON cohorts (Table 4, Table 5). Supplementation during late gestating first-calf beef heifers yielded similar outcomes on cow and offspring performance from birth to weaning; however, OMG calves were 6 kg heavier when compared with CON after being fed a high-energy feedlot diet, meaning that ɷ-3 PUFAs supplemented during late gestation might be an alternative to optimize feedlot responses of the offspring.

Fatty acid preference for beta-oxidation in mitochondria of murine cultured astrocytes.

The brain utilizes glucose as a primary energy substrate but also fatty acids for the β-oxidation in mitochondria. The β-oxidation is reported to occur mainly in astrocytes, but its capacity and efficacy against different fatty acids remain unknown. Here, we show the fatty acid preference for the β-oxidation in mitochondria of murine cultured astrocytes. Fatty acid oxidation assay using an extracellular flux analyzer showed that saturated or monosaturated fatty acids, palmitic acid and oleic acid, are preferred substrates over polyunsaturated fatty acids like arachidonic acid and docosahexaenoic acid. We also report that fatty acid binding proteins expressed in the astrocytes contribute less to fatty acid transport to mitochondria for β-oxidation. Our results could give insight into understanding energy metabolism through fatty acid consumption in the brain.

Association between dietary fat intake and risk of polycystic ovary syndrome

To analyze the association between dietary fat intake and the risk of polycystic ovarian syndrome(PCOS). PCOS patients treated in a tertiary hospital in Anhui Province from October 2021 to October 2022 were selected as the case group, and non-PCOS patients treated in the hospital during the same period were selected as the control group. A total of 262 subjects were included in the study, 131 were included in the case group and 131 in the control group. A semi-quantitative dietary frequency questionnaire was used to investigate the dietary intake in the past year, and the daily intake of various fatty acids and the ratio of fatty acid energy supply were calculated according to the food intake. Logistic regression analysis was used to investigate the association between dietary fat intake and the risk of PCOS. The dietary intakes of total fat, fatty acid, saturated fatty acid and monounsaturated fatty acid in PCOS patients were higher than those in control group(P>0.05), and there was statistical significance in daily intakes of eicosapentaenoic acid between two groups(P<0.05). After adjusting for confounding factors such as long-term residence, occupation, family per capita monthly income, menstrual cycle regularity, menstrual volume, and weight loss experience, Logistic regression analysis showed that the ratio of fat supply to energy was positively correlated with the risk of PCOS(OR=1.622, 95%CI 1.237-2.127). The energy supply ratio of monosaturated fatty acids(OR=0.597, 95%CI 0.373-0.955) and polyunsaturated fatty acids(OR=0.585, 95%CI 0.372-0.921) were negatively correlated with the risk of PCOS(P<0.05). The energy supply ratio of fat was positively correlated with the risk of PCOS, while the energy supply ratio of monosaturated fatty acids and the energy supply ratio of polyunsaturated fatty acids were negatively correlated with the risk of PCOS.

Association of Altered Plasma Lipidome with Disease Severity in COVID-19 Patients.

The severity of COVID-19 is linked to an imbalanced immune response. The dysregulated metabolism of small molecules and bioactive lipids has also been associated with disease severity. To promote understanding of the disease biochemistry and provide targets for intervention, we applied a range of LC-MS platforms to analyze over 100 plasma samples from patients with varying COVID-19 severity and with detailed clinical information on inflammatory responses (>30 immune markers). This is the third publication in a series, and it reports the results of comprehensive lipidome profiling using targeted LC-MS/MS. We identified 1076 lipid features across 25 subclasses, including glycerophospholipids, sterols, glycerolipids, and sphingolipids, among which 531 lipid features were dramatically changed in the plasma of intensive care unit (ICU) patients compared to patients in the ward. Patients in the ICU showed 1.3-57-fold increases in ceramides, (lyso-)glycerophospholipids, diglycerides, triglycerides, and plasmagen phosphoethanolamines, and 1.3-2-fold lower levels of a cyclic lysophosphatidic acid, sphingosine-1-phosphates, sphingomyelins, arachidonic acid-containing phospholipids, lactosylceramide, and cholesterol esters compared to patients in the ward. Specifically, phosphatidylinositols (PIs) showed strong fatty acid saturation-dependent behavior, with saturated fatty acid (SFA)- and monosaturated fatty acid (MUFA)-derived PI decreasing and polystaturated (PUFA)-derived PI increasing. We also found ~4000 significant Spearman correlations between lipids and multiple clinical markers of immune response with |R| ≥ 0.35 and FDR corrected Q < 0.05. Except for lysophosphatidic acid, lysophospholipids were positively associated with the CD4 fraction of T cells, and the cytokines IL-8 and IL-18. In contrast, sphingosine-1-phosphates were negatively correlated with innate immune markers such as CRP and IL-6. Further indications of metabolic changes in moderate COVID-19 disease were demonstrated in recovering ward patients compared to those at the start of hospitalization, where 99 lipid species were altered (6 increased by 30-62%; 93 decreased by 1.3-2.8-fold). Overall, these findings support and expand on early reports that dysregulated lipid metabolism is involved in COVID-19.

Abstract A058: Ether phospholipids are required for mitochondrial reactive oxygen species homeostasis

Abstract Mitochondria are hubs where bioenergetics, redox homeostasis, and anabolic metabolism pathways integrate through a tightly coordinated flux of metabolites. The essential contributions of mitochondrial metabolism to fuel tumor growth and resistance to therapy are now evident in multiple contexts. However, targeting of mitochondrial metabolism in the clinical setting has repeatedly failed, owing to a narrow therapeutic window and the remarkable ability of tumor cells to activate compensatory metabolic programs. Thus, it is essential to uncover additional mechanisms that may render cancer cells selectively susceptible to treatment and those that enable them to survive when exposed to mitochondrial metabolism-targeted drugs. Here, using pancreatic ductal adenocarcinoma (PDAC) as a disease model, we report that cellular and, in particular, mitochondrial lipid composition affects the sensitivity of cancer cells to pharmacological inhibition of electron transport chain complex I. Metabolomic, including lipidomic, analyses of patient-derived PDAC models uncovered a critical role for monosaturated fatty acids (MUFAs) and demonstrated that MUFA-linked ether phospholipids have a critical role to sustain homeostasis of mitochondrial reactive oxygen species (ROS). Blocking de novo ether phospholipid biosynthesis in the peroxisomes, sensitized PDAC cells to mitochondrial complex I inhibition by inducing mitochondrial ROS and lipid peroxidation. Biochemical analysis revealed a role of ether phospholipids in the assembly of mitochondrial supercomplexes and ROS production. Together, our data identify an ether phospholipid-dependent mechanism that regulates mitochondrial redox control and contributes to the sensitivity of PDAC cells to complex I inhibition. These findings might lead to novel approaches to target mitochondrial metabolism in cancer cells. Citation Format: Ziheng Chen, I-Lin Ho, Giulio Draetta, Haoqiang Ying. Ether phospholipids are required for mitochondrial reactive oxygen species homeostasis [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Pancreatic Cancer; 2023 Sep 27-30; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(2 Suppl):Abstract nr A058.

Comparative Investigation of Fatty Acids in the Mauritania sardinella and Moroccan Sardine

Aims: The present study was carried out to evaluate the quality and quantity of lipids and fatty acids in Mauritanian sardines (Sardinella aurita) and Moroccan sardines (Sardina pilchardus). Methods: Gas chromatography/mass spectrometry (GC/MS) and Fourier transform infrared (FTIR) analyses were adopted thereof. Results: the studied samples were found to be very rich in lipids: 17-26% for Mauritanian sardinella and 45-48% for Moroccan sardine. Thus, GC/MS analyses revealed that the fatty acids contents in both samples were subdivided into three essential groups: polyunsaturated, monounsaturated and saturated fatty acids. The semi-quantitative analysis results, calculated using chromatographic peak areas, have shown that Moroccan sardine is very rich in polyunsaturated fatty acids (mainly docosahexaenoic (DHA) and ecosapentaenoic (EPA) acids) and monounsaturated fatty acids (mainly oleic acid), compared to Mauritanian sardinella, who is very rich in monosaturated fatty acids (mainly palmitoleic acid). Conclusion: The lower valuable lipid content of sardinella was probably due to sampling conditions realized during the reproduction period. On the other hand, the omega-6/omega-3 ratio is higher in Mauritania sardinella than in Moroccan sardine.

Differential Interventional Effects of Omega-6 and Omega-3 Polyunsaturated Fatty Acids on High Fat Diet-Induced Obesity and Hepatic Pathology.

Current Dietary Guidelines for Americans recommend replacing saturated fat (SFA) intake with polyunsaturated fatty acids (PUFAs) and monosaturated fatty acids (MUFAs) but do not specify the type of PUFAs, which consist of two functionally distinct classes: omega-6 (n-6) and omega-3 (n-3) PUFAs. Given that modern Western diets are already rich in n-6 PUFAs and the risk of chronic disease remains high today, we hypothesized that increased intake of n-3 PUFAs, rather than n-6 PUFAs, would be a beneficial intervention against obesity and related liver diseases caused by high-fat diets. To test this hypothesis, we fed C57BL/6J mice with a high-fat diet (HF) for 10 weeks to induce obesity, then divided the obese mice into three groups and continued feeding for another 10 weeks with one of the following three diets: HF, HF+n-6 (substituted half of SFA with n-6 PUFAs), and HF+n-3 (substituted half of SFA with n-3 PUFAs), followed by assessment of body weight, fat mass, insulin sensitivity, hepatic pathology, and lipogenesis. Interestingly, we found that the HF+n-6 group, like the HF group, had a continuous increase in body weight and fat mass, while the HF+n-3 group had a significant decrease in body weight and fat mass, although all groups had the same calorie intake. Accordingly, insulin resistance and fatty liver pathology (steatosis and fat levels) were evident in the HF+n-6 and HF groups but barely seen in the HF+n-3 group. Furthermore, the expression of lipogenesis-related genes in the liver was upregulated in the HF+n-6 group but downregulated in the HF+n-3 group. Our findings demonstrate that n-6 PUFAs and n-3 PUFAs have differential effects on obesity and fatty liver disease and highlight the importance of increasing n-3 PUFAs and reducing n-6 PUFAs (balancing the n-6/n-3 ratio) in clinical interventions and dietary guidelines for the management of obesity and related diseases.

Long-matured cured meats from Poland and Europe compared – An overview

This review aimed to present different varieties of long-matured cured meats, such as cold cuts, sausages, and hams produced in Poland, and compare them with similar European products. Moreover, we briefly presented the impact of meat products on human health. Due to its significant production in Poland, most meat products are made of pork. Polish long-matured, cured meats are nutritious, provide essential amino acids and micronutrients, and are a balanced source of monosaturated fatty acids (MUFAs). We suggest choosing cured Polish meat products matured with probiotics to provide pro-health benefits. Although long-matured sausages are essential and valuable meat products in Poland, they should be consumed moderately as an element of a balanced, diverse diet.

Sensory Evaluation and Fatty Acid Composition of Broiler Chickens Fed Diets Containing with Prosopis Africana Essential Oil

Exploring the use of phytogenic components in plants is one of the ways to ensure safety in food and sustainable livestock production. This experiment was designed to investigate the sensory evaluation and fatty acid composition of broiler chickens fed diets containing Prosopis africana essential oil. A total of 540 – one- day- old broiler chicks (Arbo Acres) were randomly distributed into 6 treatments, each containing six replicates of 15 birds. Experimental diets were adequate in all nutrients (NRC, 1994). Birds in treatment one was fed basal diet without Prosopis africana oil, basal diet with antibiotic growth promoter (oxytetracycline at 2.5 g/kilogram) was fed to treatment two while treatment three, four, five and six were fed basal diet supplemented with 200 mg, 400 mg, 600 mg and 800 mg per kilogram respectively. Completely randomized design was used and the experiment lasted for 56 days. Feed and fresh water were offered ad libitum and other management practices were rigorously followed. Result revealed that the composition of total monosaturated fatty acid and total polyunsaturated fatty acid were significantly (P˂0.05) influenced by the treatments. Total saturated fatty acid (TSFA) levels decrease as the level of Prosopis africana oil increases across the treatments (P˂0.05). TSFA values were highest in treatment 1, intermediate in treatment 2 and lowest in treatment three through six. Omega 3: omega 6 fatty acid and artheriogenicity index were significantly (P˂0.05) different among the treatments. Prosopis africana oil also influenced color, flavor, tenderness, juiciness, texture, and general acceptability of broilers meat. It was concluded that the dietary supplementation of Prosopis africana oil has a number of phyto-constituents that can modify the fatty acid of meat, enhancing its quality, shelf life and sensory attributes. Without having a negative impact on the health of birds. It can be added to the diet of broiler chickens up to 800 mg per kilogram.

Dietary carbohydrate quality index (CQI), cardio-metabolic risk factors and insulin resistance among adults with obesity

BackgroundMetabolic syndrome (MetS), as a cluster of cardiometabolic risk factors, is a global public health concern due to its increasing prevalence. Considering the previous evidence of the association between carbohydrate quality and cardiometabolic risk factors, our study was aimed to evaluate any possible association between carbohydrate quality index (CQI) and cardiometabolic risk factors among obese adults.MethodsIn this cross-sectional study, 336 apparently healthy individuals with obesity were participated. Dietary intake was assessed by a semi-quantitative Food Frequency Questionnaire (FFQ), including 168 food items validated for the Iranian population. CQI was calculated with three components of solid carbohydrates to total carbohydrates ratio, dietary fiber intake, and dietary glycemic index (GI). Body composition was determined by bioelectrical impedance analysis (BIA). Blood pressure was measured by sphygmomanometer and enzymatic methods were used to evaluate serum lipid, glucose, and insulin concentrations.ResultsSubjects in the third quartile of CQI had significantly lower systolic blood pressure (SBP) (P = 0.03) and diastolic blood pressure (DBP) (P = 0.01). Participants in the higher quartiles of CQI had more intake of energy, carbohydrates, fat, saturated fatty acid (SFA), and mono-saturated fatty acid (MUFA) (P < 0.05). Moreover, the homeostasis model assessment of insulin resistance (HOMA-IR) was decreased in the second quartile of CQI [odds ratio (OR) = 0.146, P = 0.01) after adjustment for age, body mass index (BMI), sex, physical activity, socioeconomic status (SES) and energy intake.ConclusionAccording to our findings, a higher quality of dietary carbohydrates, determined by CQI, could be associated with a lower risk of hypertension.

Effect supplementation of black soldier fly larvae oil (Hermetia illucens L.) calcium salt on performance, blood biochemical profile, carcass characteristic, meat quality, and gene expression in fat metabolism broilers

This study evaluated the effect supplementation of black soldier fly larvae oil calcium salt (BSFLO-SCa) on performance, blood biochemical profile, carcass characteristic, meat quality, and gene expression in fat metabolism broiler chickens. A total of 280 male New Lohmann strain MB 202 broiler chicks (1-day-old) were randomly placed into 4 treatments, including a control group (T0) were fed basal diet and a basal diet supplemented with 1% (T1), 2% (T2), and 3% (T3) BSFLO-SCa. Each treatment consisted of 7 pens with 10 chickens each. Results showed that 1% BSFLO-SCa supplementation significantly reduced (P < 0.05) abdominal and meat fat, while gene expression on fat synthesis (FAS, ACC) was downregulated. Meat fatty acid profiles such as medium-chain fatty acid being dominant in lauric and myristic and monosaturated fatty acid significantly increased (P < 0.05). On the other hand, polyunsaturated fatty acid significantly decreased (P < 0.05). In addition, the other parameters did not affect by supplementation of 1% BSFLO-SCa. The addition starting from 2% significantly reduced (P < 0.05) performance and carcass characteristics. Blood biochemical profiles (HDL, protein, albumin) and meat qualities (protein, cholesterol, water-holding capacity, cooking losses, a* (redness), and b* (yellowness) values) were significantly increased (P < 0.05), while gene expression on fat oxidation (CPT-1) was upregulated. In conclusion, broiler chicken that received of 1% BSFL-SCa does not negatively affect growth performance and carcass characteristics but reduced fattening in broiler meat.

Chemical Composition of Four Industrial Hemp (Cannabis sativa L.) Pollen and Bee Preference.

Apart from its economic value, industrial hemp (Cannabis sativa L.) is a prolific pollen producer, serving as a food source for bees. However, little is known regarding the extent to which varietal differences in hemp pollen chemistry influences bee preference. Here, we report the chemical profile of pollen from four hemp varieties (Canda, CFX-2, Henola, and Joey) and bee abundance and diversity, using direct visual counts and pan traps. The number and type of bees on each variety was recorded and the chemical composition (proximate analysis and mineral, amino, and fatty acid profiles) of pollen from each hemp variety was determined. During the entire sampling period, three bee types (bumble bees, honey bees, and sweat bees) were recorded, with a combined total of 1826. Among these, sweat bees and bumble bees were the most prevalent and were highest on the Joey variety. The four varieties expressed protein content ranging from 6.05% to 6.89% and the highest in Henola. Seventeen amino acids were expressed in all varieties, with leucine recording the highest content ranging from 4.00 mg/g in Canda to 4.54 mg/g in Henola. In general, Henola expressed high protein, amino acid, and saturated and monosaturated fatty acid contents and recorded significantly fewer bees compared with Joey, which had a low content of these components and a high content of polyunsaturated fatty acids. Our findings suggest that, while industrial hemp offers abundant and accessible pollen that would promote bee health and sustainability of their ecosystem services, the nutritional quality may not be adequate for bee growth and development as an exclusive pollen source.

Emulsifying properties, in vitro digestive characteristics, and β-carotene bioaccessibility of mandarin peel pectin emulsions prepared with different carrier oil phases

Mandarin peel pectin (MPP) emulsions were prepared with different oil phase loadings with or without β-carotene, and their emulsifying characteristics, digestive properties and β-carotene bioaccessibility were investigated. Results revealed that all MPP emulsions exhibited good loading efficiency for β-carotene, while their apparent viscosity and interfacial pressure (π) of MPP emulsions increased significantly after the addition of β-carotene. Emulsification of MPP emulsions as well as digestibility were significantly dependent on the kind of oil. MPP emulsions prepared with long-chain triglycerides (LCT) oil (soybean, corn, and olive oil) exhibited higher volume average particle size (D4,3), apparent viscosity, π values, and bioaccessibility of carotene compared to those prepared with medium-chain oils (MCT). MPP emulsions with LCT rich in monosaturated fatty acids (olive oil) had the highest β-carotene encapsulation efficiency, bioaccessibility, etc. than from other oils. This study provides a theoretical basis for the efficient encapsulation and high bioaccessibility of carotenoids with pectin emulsions.

Dioscin integrates regulation of monosaturated fatty acid metabolism to extend the life span through XBP-1/SBP-1 dependent manner

Delay aging, especially in healthy life extension, brought the most interest to the medical field. Searching for anti-aging drugs with relative safety profiles bring natural products in hotspot. In this study, we find that dioscin promotes the health span extension in wild-type Caenorhabditis elegans. Through the genetic screening in C.elegans, we further reveal that dioscin activates the transcription factor SBP-1/SREBP by the UPRER transcription factor XBP-1 to upregulate transcription of the Δ9 desaturase FAT-5 and FAT-7, resulting in increased monounsaturated fatty acid content which requires for healthy life span extension. Intriguingly, through tissue-specific knockdown, we find that dioscin modulates the health span by activating SBP-1 in the intestine. Unexpectedly, dietary supplementation of POA and OA rescues XBP-1, SBP-1 mutants-induced shortened life span phenotype. Considering the conservation of MUFAs metabolism, dioscin may promote health span in other species, including mammals. Our work suggests that dioscin might be a promising candidate for developing anti-agingagent.

A broad spectrum of host plant responses to the actions of the gall midge: case study of Robinia pseudoacacia L. and Obolodiplosis robiniae (Haldeman)

This study aims to provide insights into plant-insect interaction during the formation and development of open gall structure on the leaves of Robinia pseudoacacia during gall formation by Obolodiplosis robiniae. This was the first time such far-reaching studies were performed at a biochemical and anatomical level. The gall wall is created from a few thick cells covered with epidermis. This parenchymatous nutritive tissue is rich in starch. Sclerenchyma only occurs around the vascular bundles as a result of the lignification of the parenchyma of the bundle sheaths. The level of reactive oxygen species (ROS) in the new structure was reduced and catalase activity was inhibited, which suggests another pathway of ROS decomposition – e.g. by ascorbate or glutathione peroxidase. The gall structure was combined with an increasing level of protein and non-protein thiols. Phenols seems to be a good protective factor; whose level was lower in infected leaflets. Levels of MUFA (monosaturated fatty acids) and SFA (saturated fatty acids) rose, probably as source of food for insects. The amount of fatty acid is positively correlated with the plant response. We detected that non infected leaflets produced C6:0 (hexanoic acid) and C8:0 (octanoic acid) fatty acids connected with odor. Changes in gall color as they develop are connected with photosynthetic pigments degradation (mainly chlorophylls) where the pathway of astaxanthin transformation to fatty acid is considered to be the most important process during gall maturation. Nutritive tissue is composed mainly of octadecanoic acid (C18:0) – a main source of food for O. robiniae.

Effect of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat

This study was conducted to evaluate the effects of Trichilia monadelpha stem bark extract on the fatty acid composition of rabbit’s thigh meat. A total of 40, 6-7 weeks New Zealand white × Chinchilla crossbred rabbits with an average weight of 490 ± 4.40 were randomly divided to 4 groups with 5 replications consisting of 2 animals each in a completely randomized design. Rabbits in treatment 1 (T1) was fed basal diet with no Trichilia monadelpha stem bark extract (TMSB), T2, T3 and T4 were fed basal diet with 3mL, 6mL and 9mL TMSB per rabbit/day. Phytochemical analysis of TMSB revealed the presence of tannins (10.95 mg/g), alkaloids (9.22 mg/g), saponins (4.75 mg/g), oxalates (3.10 mg/g), flavonoids (15.88 mg/g), phenols (18.46 mg/g), terpenoids (8.62 mg/g), glycosides (7.11 mg/g) and 2-diphenyl 1-piccrlhydrazyhydrate (430.8 mg/g). Concentrations of vitamins identified in the extract are; vitamin A (2.190 mg/100g), vitamin B1 (0.567 mg/100g), vitamin B2 (0.301 mg/100g), vitamin B3 (0.227 mg/100g), vitamin B9 (0.080 mg/100g), vitamin B12 (0.209 mg/100g) and vitamin C (5.680 mg/100g). Composition of saturated fatty acid (TSFA) decrease with a significant increase in monosaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) (P˂0.05) across the treatments. It was found that TMSB significantly (P˂0.05) influenced the concentration of total unsaturated fatty acid (TSFA). Therefore, it can be concluded that TMSB is capable of modulating the fatty acid and improving the quality of meat from rabbit without causing any deleterious effect on the performance of the animal.

Nutritional composition of black soldier fly (Hermetia illucens) reared on chicken waste meal, fruit &amp; vegetable waste, and their mixture

Fruits and vegetables are wasted in huge amounts and nearly 30% of all produced food is never consumed which leads to many economic and environmental issues. Similarly, the poultry industry generates a huge volume of waste. This refuse is reported to have high nutritional value and could be used as ingredients in the feed industry. Black soldier flies,Hermetia illucens, are potentially capable of converting different organic wastes into protein-rich biomass and considered as a viable alternative protein source to partially substitute fish meal used in aquafeeds, thereby contributing to the sustainability of aquaculture. In this study, the effect of two different organic waste substrates and their mixture, namely chicken waste meal (C), fruit &amp; vegetable waste (FV), and a combination (1:9) of chicken waste meal and fruit &amp; vegetable waste (M) on the nutritional content of black soldier fly prepupae (BSFP) was investigated. Significant differences were observed in energy content and proximate composition of the substrates which significantly altered the energy content and nutritional composition of the BSFP. BSFP reared on FV were low in crude protein, crude lipid and high in crude carbohydrate compared to BSFP reared on C and M. The amino acid contents of BSFP were characterised by high levels of lysine, leucine, and valine. Significant differences were detected between BSFP groups in terms of amino acid content. The fatty acid composition of the BSFP were dominated by the saturated fatty acids (SFA) ranging between 41.8±0.10% (M) and 67.11±0.08% (C). M-fed BSFP had significantly higher SFA, monosaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) compositions than C- and FV-fed BSFP. M-fed BSFP had a high C18:1n9 (17.01±0.02%), C16:0 (17.02±0.09%), C18:2n-6 (14.63±0.02%), and C12:0 (15.19%) fatty acid composition. The fatty acid profile of the C-fed BSFP was characterised by high levels of C14:0 (28.16±0.11%), C12:0 (25.79±0.09%), and C18:1n-9 (13.41±0.03%), whereas FV-fed BSFP was characterised by high levels of C16:0 (19.33±0.08%), C12:0 (16.64±0.18%), and C18:1n-9 (12.51±0.04%). Our results demonstrated that chicken waste meal, fruit &amp; vegetables waste, and their mixture can be used to feed BSFP for sustainable production. However, M-fed BSFP could be used to partially substitute fish meal in carnivorous fish feeds for having a substantially greater nutritional value than BSFP reared on other wastes and for its similarity to fish meal in terms of amino acid content.

TMET-03. STEAROYL-COA DESATURASE INHIBITOR SUPPRESSES IDH MUTANT GLIOMA GROWTH VIA ENHANCING LIPOLYSIS

Abstract BACKGROUND Mutant isocitrate dehydrogenase (IDH) produces 2-hydroxyglutarate (D2HG) and causes metabolic reprograming, but so far, little is known about the role of mutant IDH and D2HG in de novo lipogenesis and fatty acids synthesis. In this study, to develop a feasible drug for IDH mutant glioma, we targeted Stearoyl-CoA desaturase 1 (SCD1) catalyzing the biosynthesis of monosaturated fatty acids (MUFA) to suppress IDH mutant glioma progression. Materials and METHODS We prepared genetically engineered glioma cell lines (U251 wild type: U251WT and U251 IDHR132H mutant: U251RH), normal human astrocytes (empty vector induced-NHA: NHAEV and IDHR132H mutant: NHARH) and patient derived cell lines. Lipid metabolic analysis was conducted by using LC-MS, and functional analysis for the role of SCD1 expression was investigated by RNA sequence and Western-blotting. RESULTS LC-MS analysis of extracted Endoplasmic Reticulum revealed that there was significantly higher amount of MUFA in IDH mutant than wild type. SCD1 expression was increased in IDH mutant compared to wild type due to D2HG-mediated upregulation of SCD1 in IDH mutant. Therefore, IDH mutant in which SCD1 expression level was higher than wild type indicated high sensitivity to SCD inhibitor, and apoptosis was highly induced in IDH mutant compared to wild type. RNA sequencing was performed in U251RH treated with SCD inhibitor compared to U251RH treated with DMSO, and lipid droplet metabolism-associated RNA expression was significantly changed in SCD inhibitor-treated U251RH. Based on the RNA sequence data, we checked lipid droplet in U251RH with presence or absence of SCD inhibitor, and lipolysis was induced by SCD inhibitor treatment, suggesting that SCD inhibition is associated with the apoptosis in IDH mutant via enhanced lipolysis mechanism. CONCLUSIONS D2HG produced in IDH mutant glioma directly induces SCD1 expression and enhances sensitivity to SCD inhibitor, which suggests that SCD inhibitor would be IDH mutant glioma-specific treatment strategy.