Proper light curing is crucial for the success of restorative dentistry and to bond brackets to teeth, yet the responsibility is often delegated to dental assistants (DAs). This study assessed the proficiency and maintenance protocols of DAs when using light-curing units (LCUs) in Saudi Arabia. Self-administered questionnaires were distributed to practicing DAs. The questionnaire contained sections on knowledge, adherence to best practices, and how to maintain the LCU. Demographic data were collected as well. Descriptive statistics and linear regressions at a significance level of (p = 0.05) were conducted to identify any relationships that influenced the DAs' LCU knowledge, practice, and maintenance protocols. Among the surveyed DAs, 66% were responsible for using the LCU during treatment, 16% used their fingers for support when light curing, 50% held the LCU tip 1-2 mm from the restoration during curing, and 51% did not have a specific maintenance protocol at their workplace. 70% did not know the output from the LCU, and their educational background correlated with knowledge (b = -14.42, p < 0.001). The type of institution type correlated with adherence to best practices (b = -13.65, p = 0.011), and level of knowledge and adherence to maintenance protocols showed a direct correlation (b = 0.002, p = 0.041). The findings revealed that a significant percentage of the DAs who replied had insufficient knowledge and did not follow the best practices and maintenance protocols for the LCUs they were using. Their educational background and workplace factors influenced this knowledge gap, while the absence of a maintenance protocol and suboptimal practices were associated with the type of institution. To maintain the best practice, clinicians and institutions should improve the education and training of DAs. Otherwise, inadequate light curing by the DAs may jeopardize the long-term success of many dental procedures.