ABSTRACT Mastitis is a multi-etiological complex disease which adversely affects animal health, and economics of milk production of dairy herds in developing and developed countries. A variety of non-genetic factors affect the incidence of clinical mastitis. In this study, we tried to investigate the effect of four non-genetic factors viz., stage of lactation, level of milk production, parity and season on incidence of clinical mastitis in Karan Fries and Sahiwal cows maintained under similar management conditions. In Karan Fries cattle, the percentage of incidence of mastitis was maximum in fourth parity (41.1%). Similarly, rainy season and high level of milk production showed maximum percentage of incidence of clinical mastitis viz., 30.5% and 31.0%, respectively. In Sahiwal cows, the percentage of incidence of mastitis was maximum in fourth parity (52.8%), rainy season (30.4%) and high level of milk production (31.3%). Stage of lactation had no significant effect (P< 0.05) on incidence of clinical mastitis in both breeds. Therefore, it can be inferred that high-producing animals are more prone to mastitis during rainy season due to droplet infection, damp and muddy floors. Secondly, animals in advance parity were also more affected indicating that widened teat canal and elongated teat shape predisposes animal to mastitis.