Abstract
Abstract The purpose of this research is to determine the incidence and occurrence of aflatoxin M1 (AFM1) in 104 samples of milk, white cheese, kashar and butter in Sakarya, Turkey. Followed by immunoaffinity column (IAC) purification, high performance liquid chromatography (HPLC)-fluorescence detection method was implemented to analyze the samples. Results show that 61.5% of milk, 40% of white cheese, 65.4% of kashar and 29.6% of butter contains AFM1. In 53 of samples (50.96%) are not determined AFM1. The amount of AFM1 in 51 samples (49.04%) ranges from 2.4 to 47.8 ng/L/kg. AFM1 levels do not exceed the limit set by Turkish regulations (50 ng/L/kg). However, the incidence of AFM1 contamination is a potential health risk for customers, particularly for children and infants. These results emphasize the significance of constant monitoring of AFM1 levels in milk and dairy products (MDPs) in Sakarya, Turkey.
Highlights
Considered a milk toxin, aflatoxin M1 (AFM1) is the main hydroxylated metabolite of aflatoxin B1 (AFB1) in liver due to cytochrome enzymes and detected in milk and dairy products (MDPs) (Fallah et al, 2009)
high performance liquid chromatography (HPLC) analysis results show that the recovery rate of the analyte in spiked samples ranges from 83.20 to 104.01% and a relative standard deviation (RSD) varying between 0.51 and 2.40%
The results show that milk and dairy samples are contaminated with AFM1
Summary
Considered a milk toxin, aflatoxin M1 (AFM1) is the main hydroxylated metabolite of aflatoxin B1 (AFB1) in liver due to cytochrome enzymes and detected in milk and dairy products (MDPs) (Fallah et al, 2009). Varying according to AFB1 contamination level in feeds, around 0.3 to 6.2% of AFB1 consumed by dairy animals is chemically modified into AFM1 (Ayar et al, 2007). The incidence of AFM1 in MDPs poses a potential health risk for animals and humans in general and for susceptible population groups such as infants and young children in particular (Piva et al, 1988). AFM1 causes dairy animals to consume less feed and hinders their development (Beasley, 2011). Despite the fact that AFM1 has lower genotoxic activity than AFB1, the latter is considered by the International Agency for Research on Cancer (IARC) to be carcinogenic to humans (Group 2B). Contamination levels and distribution variations of AFM1 vary according to season and location (Aliabadi et al, 2012)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
