Left Injury Research Articles

Performance of Bt-susceptible and -heterozygous dual-gene resistant genotypes of Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in seed blends of non-Bt and pyramided Bt maize.

A seed blend refuge has been implemented in the U.S. Corn Belt for Bt maize resistance management. The fall armyworm, Spodoptera frugiperda (J.E. Smith), is a target pest of Bt maize in the Americas. The larvae of this pest are mobile, which may affect the efficacy of seed blend refuges. In this study, field and greenhouse trials were conducted to determine the performance of Bt-susceptible (aabb) and -heterozygous dual-gene-resistant (AaBb) genotypes of S. frugiperda in seed blends of non-Bt and pyramided Bt maize. Three field trials evaluated larval survival, larval growth, and plant injury with aabb in seed blends of Bt maize expressing Cry1A.105/Cry2Ab2/Vip3A with 0-30% non-Bt seeds. Greenhouse tests investigated the performance of aabb and AaBb in seed blends of Cry1A.105/Cry2Ab2 with 0-30% non-Bt seeds. In pure non-Bt maize plots, after 9-13 d of neonates being released on the plants, 0.39 and 0.65 larvae/plant survived with leaf injury ratings of 4.7 and 5.9 (Davis's 1-9 scale) in the field and greenhouse, respectively. In contrast, live larvae and plant injury were virtually not observed on Bt plants across all planting patterns. Larval occurrence and plant injury by aabb on non-Bt plants were similar between seed blends and pure non-Bt plantings, suggesting that the blended refuges could provide an equivalent susceptible population as structured refuge under the test conditions. In the greenhouse, the two insect genotypes in seed blends performed similarly, indicating that the seed blends did not provide more favorable conditions for AaBb over aabb. The information generated from this study should be useful in managing S. frugiperda and evaluating if send blends could be suitable refuge options for Bt resistance management in the regions where the insect is a primary target pest.

Physiological and Biochemical Response of Rice Cultivars (Oryza Sativa L.) To Elevated Ozone

ABSTRACT A study was carried out to evaluate the effect of elevated tropospheric ozone concentration on 15 rice cultivars. The cultivars were exposed to a weighted average ozone concentration of 51 ppb ±10% (10:00–17:00 h) for 30 days on its reproductive stage. On an average, elevated ozone significantly affects photosynthetic rate (−19.23%), stomatal conductance (−21.35%) and the contents of chlorophyll (−27.19%), malondialdehyde (+121.43%), proline (+98.38%), and ascorbic acid (−20.99%) over the control. Linear multiregression 3D model significantly fits the leaf traits related to leaf injury percentage which in turn affects all photosynthetic traits. The maximum R2 = 0.85*** was observed between photosynthetic rate, chlorophyll content, and leaf injury percentage. Rice cultivars exhibited significant interspecies variation in physiological and biochemical traits under elevated ozone. Among the tested rice cultivars, the multivariate analysis confirmed the Rice CO51, Anna(R)4, CO47, PMK(R)3, and Rice TPS5 were ozone tolerant. For the first time in South India, the study on response of rice cultivars to elevated ozone was done, which might support the rice breeders for selecting suitable donor parents for future climate change scenarios.

Low Tunnels Reduce Insect Populations, Insecticide Application, and Chewing Insect Damage on Brussels Sprouts.

Low tunnels covered with spun-bonded fabric (row covers) provide season extension for vegetable production and also afford a physical barrier against airborne insects and other non-soil pests. Brussels sprouts, Brassica oleracea L. group Gemmifera (Brassicaceae), is a popular vegetable in local markets in Virginia; however, unprotected field production is severely affected by insect pest infestation. This study's objective was to determine the level of protection low tunnels provide against insect infestation and leaf herbivory injury. The experiment was conducted at the Virginia Tech Eastern Shore Agricultural Research and Extension Center in Painter, Virginia. The experimental design was split-plot with polyethylene soil mulches (white or black) as whole plot factors and production systems (low tunnel or open field) as subplot factors. In this study, low tunnels reduced insect infestation and chewing herbivory leaf injury to Brussels sprouts. Compared to an unprotected open field, infestations of lepidopteran insects and harlequin bug, Murgantia histrionica (Hahn) (Hemiptera: Pentatomidae) were reduced on plants under low tunnels. However, aphids (Hemiptera: Aphidae) infestation occurred under low tunnels in fall. There was no effect of color mulches (white or black) and no interaction between tunnel and mulch color on insect infestation and chewing injury. Fewer insect infestations and feeding injury indicate that low tunnels can be an effective management tool for sustainable vegetable production.

High temperature damage to fatty acids and carbohydrate metabolism in tall fescue by coupling deep transcriptome and metabolome analysis

High temperature damage impairs the growth of tall fescue by inhibiting secondary metabolites. Little is known about the regulation pattern of the fatty acids and carbohydrate metabolism at the whole-transcriptome level in tall fescue under high temperature stress. Here, two tall fescue genotypes, heat tolerant PI578718 and heat sensitive PI234881 were subjected to high temperature stress for 36 h. PI 578718 showed higher SPAD chloroplast value, lower EL and leaf injury than PI 234881 during the first 36 h high-temperature stress. Furthermore, by transcriptomic analysis, 121 genes were found to be induced during the second energy production phase in tall fescue exposed to high-temperature conditions, indicating that there may be one energy-sensing system in cool-season turfgrass to adapt high-temperature conditions. PI 578718 showed higher differentially expressed unigenes involved in fatty acids and carbohydrate metabolism compared with PI 234881 for 36 h heat stress. Interestingly, a metabolomic analysis using GC-MS uncovered that the sugars and sugar alcohol accounted for more than 65.06% of the total 41 metabolites content and high-temperature elevated the rate to 82.89–91.16% in PI 578718. High-temperature damage decreased the rate of fatty acid in the total 41 metabolites content and PI 578718 showed lower content than in PI 234881, which might be attributed to the down-regulated genes in fatty acid biosynthesis pathway in tall fescue. The integration of deep transcriptome and metabolome analyses provides systems-wide datasets to facilitate the identification of crucial regulation factors in cool-season turfgrass in response to high-temperature damage.

Efficiency of Pinus nigra J.F. Arnold in removing pollutants from urban environment (Plovdiv, Bulgaria).

The aim of this study was to assess the efficiency of urban vegetation in removing pollutants from the environment using Pinus nigra J.F. Arnold as a model tree. For this purpose, the concentrations of some heavy metal and potentially toxic elements (Al, As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, U, and Zn) in needles, branches, and roots of P. nigra, as well as in the surrounding soil, have been analyzed and the removal capacity has been estimated on the basis of element translocation. The relationship between ecosystem services provisioning and plant well-being was also analyzed through physiological (photosynthetic pigment content and ratio) and anatomical (leaf injuries appearance) indicators. Enrichment factor, translocation factor, bioaccumulation factor, and index of translocation were calculated in order to assess the urban impact and removal efficiency of sampled trees, as well as the general transport of trace elements between the studied plant's organs as results of wet and dry atmospheric deposition. Anthropogenic pollution with As, Cd, and Pb was proven in whole urban environment, so these high values of translocation through pine trees have given an added value to the regulating ecosystem services of urban vegetation. Searching and planting of trees with higher removal efficiency could be crucial for improving quality of urban environment.

Peanut and Weed Response to Postemergence Herbicide Tank-Mixtures Including Paraquat and Inorganic Liquid Nutrients

ABSTRACT Weed control is an integral part of peanut (Arachis hypogaea L.) production systems. Paraquat is a staple postemergence (POST) herbicide used in peanut production in the Southeast US. Inorganic liquid nutrient (ILN) concentrates are liquid fertilizers that are recommended for use by producers in tank-mixtures with paraquat by some distributors. Irrigated and non-irrigated field trials were conducted to quantify the safening effect of ILN in various herbicide tank-mixtures on peanut and determine the suitability as tank-mix replacements for bentazon. Field studies indicated similar POST herbicide responses for peanut injury. Greenhouse experiments evaluated POST paraquat tank-mixtures with ILN for weed control and biomass reduction. Paraquat plus S-metolachlor caused significant leaf burn and stunting. Greatest peanut foliar injury occurred 3 d after treatment (DAT) but was transient. For the irrigated field trial, paraquat plus S-metolachlor plus ILN had similar injury levels as compared to paraquat plus S-metolachlor plus acifluorfen plus bentazon at 22 to 25%. For the non-irrigated field study, the application of paraquat plus ILN had 10% injury compared to paraquat at 22%. While injury was the greatest directly following application, peanut was able to recover with no yield or grade loss for both the irrigated and non-irrigated studies. In the greenhouse study, the effect of ILN varied by weed species and reduced leaf injury on several broadleaf weeds. While the addition of ILN to the various paraquat tank-mixtures initially reduced injury, it did not correspond to increases in yield or grade. The variability in weed control, transient injury mitigation, and no yield increase indicates that Georgia peanut growers will receive no benefit for including ILN in their paraquat tank-mixtures but if needed to improve crop nutrition, ILN will not reduce weed control.

Zinc and Copper Enhance Cucumber Tolerance to Fusaric Acid by Mediating Its Distribution and Toxicity and Modifying the Antioxidant System.

Fusaric acid (FA), the fungal toxin produced by Fusarium oxysporum, plays a predominant role in the virulence and symptom development of Fusarium wilt disease. As mineral nutrients can be protective agents against Fusarium wilt, hydroponic experiments employing zinc (Zn) and copper (Cu) followed by FA treatment were conducted in a glasshouse. FA exhibited strong phytotoxicity on cucumber plants, which was reversed by the addition of Zn or Cu. Thus, Zn or Cu dramatically reduced the wilt index, alleviated the leaf or root cell membrane injury and mitigated against the FA inhibition of plant growth and photosynthesis. Cucumber plants grown with Zn exhibited decreased FA transportation to shoots and a 17% increase in toxicity mitigation and showed minimal hydrogen peroxide, lipid peroxidation level with the increased of antioxidant enzymes activity in both roots and leaves. Cucumber grown with additional Cu absorbed less FA but showed more toxicity mitigation at 20% compared to with additional Zn and exhibited decreased hydrogen peroxide level and increased antioxidant enzymes activity. Thus, adding Zn or Cu can decrease the toxicity of the FA by affecting the absorption or transportation of the FA in plants and mitigate toxicity possibly through chelation. Zn and Cu modify the antioxidant system to scavenge hydrogen peroxide for suppressing FA induction of oxidative damage. Our experiments could provide a theoretical basis for the direct application of micro-fertilizer as protective agents in farming.

Screening Thermal Shock as an Apple Blossom Thinning Method. I. Stigmatic Receptivity, Pollen Tube Growth, and Leaf Injury in Response to Temperature and Timing of Thermal Shock

The use of short-duration applications of thermal energy (thermal shock; TS) as an apple blossom thinning strategy was investigated. Effects of TS temperature and timing on stigmatic receptivity, pollen tube growth in vivo, and visible leaf injury were evaluated in multiple experiments on ‘Crimson Gala’. TS treatments were applied to blossoms and spur leaves using a variable temperature heat gun. TS temperatures ≥86 °C had a strong inhibitory effect on pollen tube growth on the stigmatic surface and in the style. TS temperatures >79 °C reduced average pollen tube length to less than the average style length. Timing of TS treatment (0 or 24 hours after pollination) was not an influential factor, indicating that effective TS temperatures reduced pollen tube growth up to 24 hours after the pollination event. The onset of thermal injury to vegetative tissues occurred at similar TS temperatures that inhibited pollen tube growth in vivo. Excessive leaf injury (>33%) was observed at 95 °C, suggesting relatively narrow differences in thermal sensitivity between reproductive and vegetative tissues. Inconsistent TS temperatures and/or responses were observed in some experiments. Ambient air temperature may have influenced heat gun output temperatures and/or plant susceptibility. While results suggest some promise, additional work is required to validate and further develop this concept.

Screening Thermal Shock as an Apple Blossom Thinning Method. II. Pollen Tube Growth and Spur Leaf Injury in Response to Temperature and Duration of Thermal Shock

Blossom thinning can confer significant benefits to apple growers, including increased fruit size and annual bearing. However, current blossom thinning practices can damage spur leaves and/or fruit. We evaluated the use of short duration forced heated air treatments [thermal shock (TS)] as a blossom thinning strategy for ‘York Imperial’. Using a variable-temperature heat gun, TS treatments were applied to solitary blossoms 24 hours after pollination. Effects of output temperature (five levels) and treatment duration (four levels) were evaluated using a completely randomized design with a factorial treatment structure. Short duration treatments (0.5 and 1.0 seconds) were ineffective for arresting pollen tube growth in vivo. TS temperature required to inhibit stylar pollen tube growth was inconsistent across years. In 2014, TS temperatures ≥56 °C inhibited pollen tubes from reaching the style base at 2.0 and 4.0 second durations. However, in 2015, TS temperatures ≥81 °C at 4.0 seconds prevented pollen tubes from reaching the style base. Inconsistent effects of TS across years were attributed to treatments being applied too late due to optimal conditions for pollen tube growth during the intervening 24-hour period after pollination. Excessive injury to spur leaf tissue was observed at temperatures higher than 84 °C and 70 °C (2.0 and 4.0 seconds, respectively). Pollen tube growth was reduced or arrested at temperature and duration combinations that caused minimal visible injury to spur leaves. Identifying and exploiting structural differences between apple blossoms and vegetative spur leaves may provide insight for the future development of TS or other attempts at developing selective thinning technologies.

Sensitivity of different parameters for selection of higher plants in urban afforestation: Exposure of Guabiroba (Campomanesia xanthocarpa O. Berg.) to diesel engine exhaust

Urban afforestation can mitigate the effects of air pollution by acting as a sink for atmospheric emissions, but these emissions (e.g., combustion gases from diesel engines) can be a precursor of structural and physiological changes in higher plant species, which could compromise the success of afforestation projects. In this study, Guabiroba (Campomanesia xanthocarpa O. Berg.) plants were exposed in greenhouses to combustion gases emitted by a diesel engine over 120 days, with daily intermittent gas exposure. Every 30 days, leaf injury (chlorosis and necrosis), plant biomass and physiological/biochemical parameters (proteins, chlorophyll and peroxidase enzyme activity) were evaluated. The data obtained were used to construct a hierarchy of the sensitivity (and inversely, of the resistance or tolerance) of this higher plant species to the diesel oil combustion gases: peroxidase > biomass ≈ chlorophyll > protein > leaf injury. Variations in these parameters could be used for the early diagnosis of plant stress or as a marker for stress tolerance in trees. In the first case, a sensitive species could be used for the phytomonitoring of air quality and in the second case the lack of significant variations in these parameters would indicator tolerance of the plant species to air pollution. The results showed that Guabiroba, a plant native to the Atlantic forest, is sensitive to air pollution and could therefore be used for air quality monitoring, since all parameters analyzed were affected by the polluted air.

Growth and leaf injury in tomato plants under continuous light at different settings of constant and diurnally varied photosynthetic photon flux densities

In tomato, continuous light inflicts distinctive injuries, including leaf chlorosis and epinasty, a phenomenon that is not fully understood. To gain insights regarding the mechanism underlying this phenomenon, we cultivated tomato seedlings (Solanum lycopersicum cv. Momotaro) for 14 days under continuous light with one of three constant photosynthetic photon flux densities (PPFD) (300, 175 or 50 μmol⋅m−2⋅s-1) or two diurnal variations of PPFD (300 μmol⋅m−2⋅s-1 for 14 h alternating with 50 or 5 μmol⋅m−2⋅s-1 for 10 h). We quantitatively evaluated and compared the levels of leaf chlorosis and epinasty of these plants with those grown under a 14/10-h photoperiod. The results showed that under continuous light with constant PPFD, the level of leaf chlorosis did not increase with higher light intensity, whereas the level of leaf epinasty increased with increasing light intensity, suggesting that these injuries are inflicted through different mechanisms. Relative to continuous light with constant PPFD, diurnal variations in PPFD significantly mitigated leaf chlorosis, slightly reduced leaf epinasty and improved plant growth. The leaf carbohydrate concentrations, including glucose, fructose, sucrose and starch concentrations, did not follow the trend in the level of leaf chlorosis. However, a daily pattern of increase and decrease of the sucrose content resulted from diurnal variation in PPFD, which seemed to be associated with the mitigation of continuous light injury. These results suggest that the circadian rhythm rather than the overaccumulation of carbohydrates is involved in the induction of continuous-light-induced injuries in tomato.

Reduced Glutathione Protects Subcellular Compartments From Pb-Induced ROS Injury in Leaves and Roots of Upland Cotton (Gossypium hirsutum L.).

Heavy metals-based changes in the plants and their alleviation through eco-friendly agents including reduced glutathione (GSH) have been widely studied. In the present experiment, we tested the alleviatory role of reduced glutathione (GSH) in seedlings of upland cotton cultivar, TM-1 under lead (Pb) toxicity. Plants were grown in the Hoagland solution containing Pb (0 μM), Pb (500 μM), GSH (50 μM), and GSH + Pb (50 μM + 500 μM). Lead exposure exacerbated hydrogen peroxide (H2O2) and hydroxyl radical (OH•) levels, induced lipid peroxidation (MDA), and decreased the activities of catalase (CAT) and ascorbate peroxidase (APX) in the terminal and median leaves of 28-days old cotton seedlings stressed for 10 days. However, in the primary and secondary roots, CAT activity was increased but APX decreased. Similarly, peroxidase (POD) and superoxide dismutase (SOD) activities were enhanced in the median leaves but a declining trend was observed in the terminal leaves, primary roots and secondary roots. Glutathione reductase (GR) activity, ascorbic acid (AsA) contents and GSH concentrations were increased in all parts except AsA in the median leaves. Transmission electron micrographs of Pb-treated plants exhibited deformed cell wall and cell membrane, disfigured chloroplasts and irregularly shaped mitochondria in the terminal and median leaves. Further, cell membrane, mitochondria, nucleus and other cell organelles in root cells were severely affected by the Pb. Thus their identification was little bit difficult through ultramicroscopy. External GSH stabilized leaf and root ultramorphology by stabilizing cell membranes, stimulating formation of multivesicular body vesicles, and by maintaining structural integrity of other organelles. Evidently, GSH played major alleviatory role against Pb toxicity in upland cotton.

Infestation and Yield Losses Due to Sesame Webworm (Antigastra catalaunalis, (Duponchel) on Different Sesame Varieties in Western Tigray, Northern Ethiopia

Sesame (Sesamum indicum L.) is the most important oilseed crop in Ethiopia. It is basically grown during the summer season in different regions of the country and produced mainly in the northern and northwestern lowlands of Ethiopia. The production of sesame is low because of insect pests such as sesame webworm, sesame seed bug and gall midge. Among these pests, sesame webworm is one of the most important insect pests and it attacks the crop at all growth stages and causes a significant yield loss. Therefore, the present study was aimed to assess the infestation and avoidable yield losses of sesame due to sesame webworm on different released sesame varieties at field conditions. A field experiment was conducted to assess the infestation and yield losses with respective cost-benefit of nine released sesame varieties to sesame webworm in paired plots (treated with Diazinon 60% EC and untreated) in RCBD with three replications in 2016 production year. There was a significant difference (p<0.001) between the protected and unprotected plots of each variety in leaf, flower and capsule injury. Protection of sesame from the webworm with insecticide effectively reduced leaf (0.02%), flower (<2%) and pod (<1%) damage to very low levels, On the other hand, the unprotected sesame had high levels of infestation. The highest capsule injury was recorded on BaHa-Necho (9.68%) and varieties Adi, Setit-2 and Borkena sustained <5% capsule injury. Grain yield was also increased significantly (p<0.001) in the protected plots of the nine varieties as compared to their corresponding unprotected plots. Maximum yield was obtained from Setit-2, Setit-1, Humera-1 and Higher in both treated and untreated plots. The highest avoidable yield losses were obtained from Adi, Gonder-1, BaHa-Necho, and BaHa-Zeyit with respective cost-benefit ratio 2.27, 2.02, 2.12 and 2.03. Therefore integration of sowing the varieties Setit-2, Setit-1, Humera-1 and Hirhir and spraying with Diazinon 60 EC, at a rate of one liter per hectare at 2, 4 and 6 weeks after crop emergence (WAE) has potential to reduce the yield losses of sesame due to sesame webworm infestation and to increase yield of sesame.

Injury to Southern Highbush Blueberries by Southern Red Mites and Management Using Various Miticides.

Reports of severe infestations caused by southern red mites (SRM), Oligonychus ilicis McGregor (Acari: Tetranychidae), have increased in recent years in southern highbush blueberries (SHB). Currently, there is little known about the management of tetranychids in SHB, and only two miticides (fenazaquin and fenpyroximate) have recently been labeled for use in SHB. Oligonychus ilicis has caused up to 80%–100% losses in some blueberry plantings, and growers are looking for management tools for this new pest of blueberries. We report on injury to SHB from O. ilicis and the performance of seven miticides used to manage SRM populations, including spiromesifen, spiromesifen plus surfactant, vegetable oil concentrate, fenazaquin, “proprietary miticide” (referred to as Pro1), bifenazate, and fenpyroximate. Miticide efficacy was rated based on the number of SRM recorded on collected leaves and plant damage ratings using an arbitrary index (from 0 = no bronzing to 4 = 100% bronzing). Characteristic symptoms of leaf injury included purple or bronzed leaf color, leaf dryness and roughening. Fenpyroximate significantly reduced mite numbers three days after application. Additionally, plants treated with fenpyroximate or fenazaquin showed significantly less bronzing compared with the control plants. Overall, fenpyroximate and fenazaquin showed the best performance for the management of O. ilicis on SHB.

Penetration resistance of second above ground internode in V6–10 stage maize plants confer resistance to stalk boring larvae of Chilo partellus (Swinhoe) in maize

Large scale germplasm screening for Chilo partellus resistance in maize is solely based on visual scoring of damage by leaf injury rating (LIR), where the accuracy in judging a genotype comes by experience of the person, who logically integrate certain symptoms such as levels of growth retardation. Since the pest spends more than half of its larval period damaging the stalk, a quantitative phenotyping method that gives weightage to stalk resistance is required. To explore the traits governing stalk resistance, stalk infestation behaviour of C. partellus was studied first. It was found that first instar C. partellus larva is exclusive whorl feeder, second instar is the transition stage which disperse to infest stalk, and third instar onwards are exclusive stalk borers. To access the stalk, larva tends to penetrate the second above ground internode (seventh internode of maize plant) of V6–10 stage maize. Thus, penetration resistance (PR) of seventh internode was hypothesized to be the characteristic resistance trait. Twenty elite maize breeding lines were evaluated for PR of rind (RPR) and pith (PPR) of seventh internode in V6–7, V8–9 and V10–11 stage plants by TA + Di Texture Analyzer. Significant negative correlation of LIR with RPR of all plant stages and with PPR of V6–7 stage plants suggests the ability of seventh internode to resist larval penetration would confer tolerance to C. partellus. The trait was also found a strong predictor of antibiosis, by affecting the biology and behaviour of C. partellus larva. Thus, PR of seventh internode is proposed to be the trait for phenotyping stalk resistance to C. partellus in maize, whose deployment could lead to selection of genotypes which are resistant to stalk lodging also.

Does Zygogramma bicolorata Pallister really affects the growth, density and reproductive performance of Parthenium hysterophorus L?

Zygogramma bicolorata Pallister (Coleoptera: Chrysomelidae) is a satisfactory potential candidate for controlling Parthenium hysterophorus L. The study was conducted in order to evaluate the bio-control potential of Z. bicolorata to satisfactory reduced the density of parthenium weed in natural field conditions. Therefore, beetles were released in the twelve study sites @ 12 adults and/or larvae/m2 in their respective sites on 5th, 10th, 15th and 20th day after initial field releases. Observations were made on defoliation by the beetle, perthenium plant height, plant biomass, leaf injury rating and soil seed reservoir. Present study showed that field releases of Z. bicolorata considerably lowering down the parthenium density at all treated sites. At larval release sites by the 60th day of observation a complete defoliation was noted, however at adult release sites it took 80 days for complete defoliation. Moreover, significantly higher number of developmental stages i.e. eggs, larvae and adults were noted in the adults released sites than to larval released sites and the controls. It was also noted that plant height and its density, flowers & leaves production, biomass of parthenium plant and seed reservoir in soil was negatively affected in treated sites as compared to untreated sites. Thus, we have concluded that adults can be utilized in biocontrol program because population build-up was greater in those sites where adults were released.

Chunk by Chunk Irrigation of Farm Field -Through Wireless Sensing Technique

Farming act as a heart to Indian economy and is a work of farmers. Farmers pursue certain set of stages to farm a field and irrigation is essential stage among all stages. Farmers are using so many irrigation methods to farm a field and the Irrigation methods must be in such way that, it have to boost plant development while minimizing salt inequities, leaf injuries, soil erosion, and water loss. To get good results in irrigation we should use good irrigation system. Now a days, farmers are using so many irrigation systems to pump water on the farm like traditional, modern and automated methods. Even though farmers facing problems like current shocks, standing long time and monitoring each and everything to pump water on the entire farm. In this paper modernistic sensor-based water pumping system is proposed to made irrigation easy to the farmers by chunk by chunk irrigation and each chunk is supervised with the help of soil moisture, temperate and humidity sensors. One more criteria, farmer need to concentrate while selecting an irrigation method is power supply, especially in Andhra Pradesh there is a regular power cuts in the nights.so that farmers can’t go every time to monitor how the irrigation is in the nights. Because in the nights snack and poisonous insects will be there in farm field, so it will be dangerous to the farmer’s life.so, by using proposed irrigation method irrigation will be easy in regular power cut areas, sloppy area and irrigation at night time.one more benefit from this proposed method is man power will decrease.

Evaluation of Insecticide Thresholds in Late-Planted Bt and Non-Bt Corn for Management of Fall Armyworm (Lepidoptera: Noctuidae).

The fall armyworm, Spodoptera frugiperda (J. E. Smith), is a major pest of corn in North and South America. It is managed primarily with transgenic corn-producing insecticidal proteins of Bacillus thuringiensis (Bt), but the development of resistance threatens their durability and necessitates the use of alternative management strategies. We conducted late-planted field trials during 2016 and 2017 in South Carolina using natural infestations. We evaluated the use of Bt and non-Bt corn hybrids in combination with foliar applications of chlorantraniliprole at varying infestation thresholds to protect field corn from infestation and damage and determine effects on grain yield. All Bt hybrids were more effective at reducing fall armyworm infestation rates and leaf injury than multiple insecticide sprays, and no Bt hybrid reached the lowest infestation threshold (20%) to require supplemental insecticide treatments, despite infestations in non-Bt corn reaching >68% in each year. The only Bt and/or insecticide treatment to significantly reduce ear feeding or the proportion of ears injured (mainly by Helicoverpa zea [Boddie]) was the Bt hybrid pyramid producing Vip3A. However, significant protection of yield was detected only in the Bt hybrids producing Cry1A.105 + Cry2Ab2. All Bt traits tested in this study were effective in reducing infestation and feeding damage from fall armyworm, although this did not always result in significant protection of yield. Our results demonstrate the potential and limitations of using chlorantraniliprole with Bt (when resistance is present) and non-Bt corn to manage this pest.

Response Of Heavy And Metallic Element On Lingual Limitation Of Java Plum Phanerogam

Serious metals will response s the expansion, morphology and metabolism of plants in many ways that. the current experiment was carried to see the impact of serious metals on coinage Java Plum , exploitation 2 sites; controlled and contaminated. The lingual limitation like leaf space, root-shoot length, leaf injury, and biomass of the plants were studied in each the sites and compared with one another. These serious metals in the least concentration (1mg/l, 5mg/l and ten mg/l) exhibited vital reduction in leaf space, root-shoot length, and biomass of plants. Increasing concentration of metal and Cd considerably (p<0.05) slashed the lingual parameter of plant as compared to manage. Hence, the lingual traits could serve to see appropriate bioindicator of serious metal pollution and conjointly for the classification of the species as tolerant or sensitive to serious metals.

Morphoanatomical injuries in Pistia stratiotes L. (Araceae) as a result of exposure to clomazone in water.

Contamination of water sources due to herbicide is of great concern. Clomazone is a pesticide with a high contamination potential which could possibility lixiviate to water streams. Changes caused by residual herbicide include flora modifications which are generally detrimental for some species. The lack of morphological studies performed in aquatic plants exposed to herbicide-contaminated environments has encouraged the development of our research. For the first time, we present a study that aimed to evaluate leaf injuries visible to the naked eye as well as microscopical effects which may be caused by clomazone on Pistia stratiotes. Pistia stratiotes was subjected to five concentrations of clomazone. Our analysis showed leaf injuries, especially after 15 days of clomazone application. Hormesis was observed when the water lettuce was subjected to the lower concentrations. Total leaf area showed increase following by reduction while injured until reaching the highest concentration. Although the concentrations of clomazone tested in our study are not lethal to water lettuce, such herbicide have still caused morphoanatomical damages on leaves which advocates for the use of P. stratiotes as a bioindicator of the presence of herbicides such as clomazone in water.