Aegle Marmelos Leaf Extract Research Articles

Bio-based fabrication of palladium nanoparticles by using Aegle marmelos leaf extract for biomedical applications: Cytotoxicity, cell imaging studies in HeLa cells and antioxidant activity

This paper presents the outcome of medicinal plant-derived synthesis of nanomaterial. The results in the present study provide a simple, novel, sustainable, and economically noble process for the synthesis of palladium nanoparticles using leaf extract Aegle marmelos (A. marmelos). Fourier transformed infrared spectroscopy (FTIR) was used to verify the surface layer of synthesized palladium (ALE@Pd) nanoparticles (NPs) enriched with several kinds of phytochemicals. Transmission electron microscopy (TEM) revealed that the synthesized NPs possess a ball shape and the average diameter was 4.31 ± 1.89 nm. The synthesized NPs possess stability confirmed by UV–visible (UV–Vis) spectrophotometry. The face-centered cubic (FCC) crystal structure of the fabricated nanoparticles was confirmed by powder X-ray diffraction (PXRD). Fabricated ALE@Pd NPs are subjected to further testing for their cytotoxicity in HeLa cell lines, as well as for a comparative study of antioxidant activity between the A. marmelos leaves and prepared ALE@Pd NPs. The anti-carcinogenic activity and cell imaging in HeLa cells were tested, and the minimal inhibition concentration (MIC or IC50) has been determined and found to be around 46 µg/mL for HeLa cell lines. Fabricated palladium nanoparticles derived from A. marmelos have the potential to be employed as an environmentally friendly and cost-effective anticancer agent. Furthermore, synthesized ALE@Pd NPs possess higher antioxidant activity than A. marmelos leaves extract (ALE) due to the increased phenolic content connected to the surface of produced palladium NPs as a capping agent. The minimal inhibition concentration of synthesized ALE@Pd NPs was found to be ∼5 µg/mL.

Preparation, characterization of green synthesis FeO nanoparticles and their photocatalytic activity towards Basic Fuschin dye

In the current study, a green and facile route for the synthesis of iron oxide nanoparticles (FeO-NPs) was adopted. The FeO-NPs were fabricated via a single step green route using aqueous leaf extract of Aegle marmelos (A. marmelos) as capping/reducing and stabilizing agents.The active phytochemicals present in the A. marmelos extract was reduced and stabilized the metal ions to FeO-NPs. This product was analyzed by FTIR spectral studies. Magnetic FeO-NPs surface morphology and particles sizes were determined by HRTEM to reveals spherical shapes with average diameter 18.78 nm. The instrumentally confirmed FeO-NPs were determining the antimicrobial activity against the pathogenic strains of the bacteria Streptococcus Sp and the fungus Candida Sp. The results of the microbial investigation show that when the concentration of synthesized FeO-NPs increase with the increases the activity. The antioxidant properties of synthesized FeO-NPs were evaluated by the DPPH assay. The FeO-NPs displayed a dose-dependent cytotoxicity property against breast carcinoma MDA-MB-231 cells, and the value of IC50 was found to be 304 μg/mL. The obtained FeO-NPs were used as photocatalyst in the degradation/reduction of environmentally polluted organic wastewater dyes. After 20 min of solar light irradiation shows 97 % of degradation efficiency was achieved with 1 mL of FeO-NPs. It is observed all the results, the green synthesized FeO-NPs could be effectively materials and used in several fields like industrial, agricultural, medical, food safety, and dye waste applications.

Aegle marmelos (L.) Leaf Extract Improves Symptoms of Memory Loss Induced by Scopolamine in Rats.

Alzheimer's disease (AD) is the most common neurodegenerative disease that results in memory impairment. Aegle marmelos (L.) Correa (AM) is used as a traditional medicine. AM leaves have the potential to inhibit acetylcholinesterase activity. This study used scopolamine to induce AD in rats. The aim of this study was to investigate the effects of AM leaf extract using this model. Motor and memory functions were tested by the motor activity and Morris water maze (MWM) tests, respectively. The density of the synaptophysin and dendritic spines in the CA1 were detected by immunofluorescence and Golgi impregnation, respectively. The hippocampal histology was reviewed by H&E staining. After the treatment, the latency times in the MWM tests of the AD groups reduced, while the motor activities showed no difference. The density of the synaptophysin of the AD groups increased after the treatments, and that of the dendritic spines also increased in all AD groups post-treatment. The hippocampal tissue also recovered. AM leaf extract can improve cognitive impairment in AD models by maintaining the presynaptic vesicle proteins and dendritic spines in a dose-dependent manner.

Bio-synthesized ZnO nanoparticles from Aegle marmelos assisted by microwave method for enhanced photocatalytic and antibacterial applications

Microwave-assisted green method was successfully used to synthesize ZnO nanoparticles in Nanoscale range by varying the Aegle marmelos leaf extract concentration. The existence of functional groups on the nanoparticle surface was confirmed by the FTIR spectra, which displayed distinctive vibrational modes. The nanoparticles size, shape, and surface features were discovered through morphological and structural research utilizing HRTEM, and SEM. The average particle size was found to be in the range of 18–28 nm. Under UV light irradiation, the ZnO NPs exhibited promising photocatalytic activity in the breakdown of methylene blue dye. The synthesized ZnO NPs are excellent in degrading dye to 80 % only in one hour. Furthermore, both Gram-positive and Gram-negative bacterial strains were effectively inhibited by the synthesized ZnO NPs' high antibacterial activity. Thus, characterization and promising photocatalytic and antibacterial properties point to the potential of these ZnO NPs for use in a range of environmental and biological applications.

Synthesis of Ag-Fe3O4 nanoparticles using Aegle marmelos leaf extract: Characterization and potential applications

Synthesis of Ag-Fe3O4 nanoparticles using Aegle marmelos leaf extract: Characterization and potential applications

Evaluation of anti-microbial activity and molecular docking of green synthesized copper oxide nanoparticle from aegle marmelos leaf extract

Evaluation of anti-microbial activity and molecular docking of green synthesized copper oxide nanoparticle from aegle marmelos leaf extract

Aegle Marmoles Biosynthesized Silver Nanoparticle Loaded Hydrogel for Topical Application for Wound Dressing

Nanotechnology gain significance importance in various field such as medicine, electronic, waste treatment and defence sectors. The physical, chemical and biological methods of producing and usage of nanoparticles for different end economic cost of beneficial application brought wide interest on nanoparticles. Aegle marmelos commonly known as Bael is used in ancient Indian medicine siddha and Ayurveda from time immemorial. It also considered to be most sacred plant in India. Silver Nanoparticles were synthesized with the aid of novel, non-toxic, eco-friendly biological method using Aegle marmelos leaf extract. Silver nanoparticles used in medicinal industry such as topical ointment to prevent infection against burns and wounds. Hydrogel loaded with metal nanoparticle have great importance for many applications in the biomedical and biotechnological field. In the present study, the biosynthesis of silver nanoparticles-based hydrogel of Maha vilvam (Aegle marmelos) extract for topical delivery. The usage of Aegle marmelos in the form of hydrogel with nanoparticle has extended its well-established application in the field of wound healing, tissue engineering and drug delivery. Further, the characterization study was carried out on biosynthesised silver nanoparticle loaded hydrogel by using UV-Visible spectrophotometer, Fourier Transform –Infrared Spectroscopy and SEM analysis. The antimicrobial resistance was accessed for the biosynthesised silver nanoparticle loaded hydrogel against Escherichia coli and Staphylococcus aureus, zone of inhibition was measured.

Eco-friendly green synthesis of silver nanoparticles from Aegle marmelos leaf extract and their antimicrobial, antioxidant, anticancer and photocatalytic degradation activity

The present research work, green synthesis of silver nanoparticles (Ag NPs) was synthesized from silver ions using the reducing and capping agents of Aegle marmelos leaf extract. Initially, UV–vis spectrophotometry absorption of the Surface Plasmon Resonance centre at 450 nm was confirmed the formation of Ag NPs. Preliminary phytochemical and FT-IR analysis indicate the identification of secondary metabolised flavonoids that act as the reducing and capping agent of the synthesized Ag NPs. Transmission electron microscope analysis, morphology of Ag NPs shown by transmission electron microscopy is spherical with a size range of ∼30–50 nm. The synthesized Ag NPs were investigate the in-vitro anticancer, antimicrobial and antioxidant activity, results shows the potential activity against the standard drugs. The Ag NPs also revealed the cytotoxicity against MDA-MB-231 human breast cancer cells. The MTT assay shows the IC50 values at 125 ± 4.26 μg/mL of Ag NPs compared to the untreated cells of negative control. The Ag NPs was excellent photocatalyst act as degradation of environmentally polluted Basic Fuchsin dye within 18 min.

Phytofabrication of Silver Nanoparticles using aqueous leaf extract of Aegle marmelos and its Characterization

Nanotechnology has proved a boon as it brings a revolutionary change in the world with the tremendous advancements in the field of science such as engineering, biotechnology, analytical chemistry, and agriculture. The synthesis of the metal nanoparticle is a developing area of exploration in present-day material science and innovation. Among the techniques involved in the synthesis of metallic nanoparticles, the biological methods or the green synthesis are reliable due to its cost-effective, environment friendly, and use of non-harmful materials in the processing. Therefore, the current work is focused on the biosynthesis of silver nanoparticles with the leaf extract of holy plant Aegle marmelos by utilizing 1mM silver nitrate solution. From the bio-reduction reaction mixture, the synthesis of silver nanoparticles was studied by UV-Visible spectrophotometer at the range of 200 nm to 800nm and discovered maximum absorbance at 460nm. Further, the SNPs were analyzed for the protein or other organic groups by Fourier Transformed Infrared spectrophotometer (FTIR). The dried silver nanoparticles were further characterized by utilizing a scanning electron microscope (SEM) to observe the actual size, shape, and distribution of particles. This result showed the actual size of the bioengineered silver nanoparticles ranges from 25-30nm. The EDX result showed a peak of Ag that confirmed its presence in the suspension. The main objective of this study is to develop a fast, environmentally friendly, and advantageous technique for the synthesis of silver nanoparticles. By standardizing the biosynthesis techniques, silver nanoparticles can be synthesized in bulk with a safe environment and it may be utilized for different clinical applications including control of pathogenic microorganisms and so on.

Bimetallic p-ZnO/n-CuO nanocomposite synthesized using Aegle marmelos leaf extract exhibits excellent visible-light-driven photocatalytic removal of 4-nitroaniline and methyl orange.

In the current study, the photocatalytic activity of bimetallic ZnO-CuO hetero-nanocomposite was evaluated and compared with the monometallic ZnO and CuO nanoparticles using 4-nitroaniline (4-NA) and methyl orange (MO). Bimetallic ZnO-CuO hetero-nanocomposite, ZnO, and CuO nanostructure were synthesized utilizing leaf extract of Aegle marmelos and characterized by transmission electron microscopy, X-ray diffraction, and XPS. Benefiting from the p-n heterostructures formation, bimetallic ZnO-CuO hetero-nanocomposite exhibits an excellent photocatalytic activity against 4-NA as well as MO compared to pure ZnO and CuO. In particular, bimetallic ZnO-CuO hetero-nanocomposite expressed the highest photocatalytic activity by reducing 90% of 4-NA in 20min and by degrading 96% of MO in 10min, whereas 65% reduction of 4-NA in 30min and 93% degradation of MO in 45min was exhibited by CuO and 48% reduction of 4-NA in 30min and 98% degradation of MO in 50min was exhibited by ZnO. Moreover, bimetallic ZnO-CuO hetero-nanocomposite maintains excellent photocatalytic activity even after five cycles indicating its stability as photocatalyst and reusability. Based on the experimental findings, bimetallic ZnO-CuO hetero-nanocomposite could be used as a photocatalyst for wastewater treatment with excellent regeneration efficiency.

Anticancer Activity of Leaf Hydro Ethanolic Extract of Aegle marmelos in Human Lung Cancer Cell Mediated through Caspase-3 and Caspase-9 mRNA Expression

Background: Aegle marmelos (AE) is a medicinal plant that comes under the rutaceae family and the plant was used in the past for treating many diseases and illness symptoms. The plant has many effects such as anti-diarrhoeal, antimicrobial, antiviral, radioprotective, anticancer, chemopreventive, antipyretic, ulcer healing, antigenotoxic, diuretic, antifertility and anti-inflammatory properties.
 Aim: To know the anticancer activity of hydroethanolic leaf extract of Aegle marmelos over lung cancer cells treated with caspase 3 and caspase 9 mRNA expression.
 Materials and Methods: The required chemicals were collected mainly from Canada. The lung cancer cells (A549) were collected from NCCS pune and then RNA was extracted from the cells and then the study was conducted after treating it with caspase 3 and caspase 9 mRNA expression. The cells were treated with many dosage of hydroethanolic extract of Aegle marmelos and the cell viability was noted.
 Results: The study reported that extract of Aegle marmelos has a great anticancer activity about 1 fold change over rate of 1.7 for cells treated with caspase 3 and a fold change over of 1 in caspase 9 treated lung cancer cells.
 Conclusion: The study concluded an innovative finding that the hydroethanolic leaf extract of Aegle marmelos has a great anticancer activity against lung cancer cells treated with caspase 3 and caspase 9 mRNA expression.

Effect of Aegle Marmelos Hydroethanolic Leaf Extract on Expression of Antiapoptotic Markers in Human Melanoma Cells

Background: Aegle marmelos commonly known as Bael is a herbal plant. Itis from a family called Rutaceae. It has many medicinal uses: anti-diarrheal, anti- microbial, anti-viral, anti-cancer, chemo-preventive, Ulcer healing and many others.
 Aim: To study the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells.
 Objective: The present study investigated the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells.
 Materials and Methods: DMSO and MTT chemicals were purchased from Sigma chemical Pvt Ltd. Trypsin EDTA, FBS, RPMI 1640 medium and PBS, Real time PCR kit was purchased from Canada. Human melanoma cell line (A375) was purchased from NCCS, Pune, India.
 Results: The data was analysed statistically by ANOVA and Duncan’s multiple range test with a computer based software (Graph Pad Prism version 5). The percentage of cell viability decreases with the increase in dosage of Aegle marmelos leaf extract. 
 Conclusion: The study concluded that Aegle marmelos hydro ethanolic leaf extract a novel and innovative herbal drug has a significant effect on the expression of antiapoptotic markers in human melanoma cell lines.

Cytoprotective and antioxidant potential of Aegle marmelos on cadmium-induced hepato-renal toxicity: an in vivo study

The current study investigated the protective and antioxidant potential of Aegle marmelos leaf extract against Cadmium-induced alterations in hepatic and renal tissues of BALB/c mice. Cadmium (Cd) is known for its toxicity and induction of oxidative stress even at a low level of exposure with prolonged persistence in the environment. Its metabolism and excretion depend on the endogenous antioxidant defense system, which gets compromised. For the present study thirty BALB/c mice were equally divided into six groups, namely Control (normal saline via gavage for 10 days), Cadmium (intraperitoneal dose of 5 mg/kg b.wt. for 5 days), Aegle alone (500 mg/kg b.wt. for 10 days via gavage), Pre-treatment of Aegle (500 mg/kg b.wt. for 10 days+5 mg/kg b.wt. of Cd for 5 days), Post-treatment of Aegle (5 mg/kg b.wt. of Cd for 5 days+500 mg/kg b.wt. for 10 days) and Vehicle group (0.5 mL oil/kg b.wt. through gavage for 10 days). The liver and kidney tissues of different groups were screened for oxidative stress indices, antioxidant enzyme systems, hepatic-renal biomarkers, histopathological alterations, chromosomal aberrations, and micronucleus formation. Sub-acute Cadmium exposure caused marked oxidative damage and tissue dysfunction evident from increased lipid peroxidation, liver-kidney serum function markers, and a subsequent decrease in reduced glutathione, catalase, superoxide dismutase, glutathione-S-transferase, glutathione reductase (p < 0.001). Histopathological analysis exhibited both reversible and non-reversible alterations.Additionally, a marked increase in chromosomal aberrations and micronucleation was observed. Both pre-and post-treatments with Aegle significantly restored altered antioxidant enzyme status, serum function markers, histomorphological alterations and reduced the percentage of chromosomal aberrations and micronucleation in the treated groups. Aegle exerts its protective efficacy due to its rich bio-active phytoconstituents, which regulated Cadmium evoked reactive oxygen species (ROS) and enhanced endogenous defense system to combat the cyto-genotoxic alterations. Moreover, all the parameters profoundly indicate higher efficacy of the pre-treatment dose.

An immunohistochemical and biochemical evidences of pancreatic β-cell regeneration in type 2 diabetic rats after treated with Aegle marmelos leaf extract and Aegeline

The study aims to investigate the Immunohistochemical changes in pancreatic beta cells in fructose fed, streptozocin (STZ) induced Type 2 Diabetes (T2DM) rats treated with various doses of leaf extract of Aegle marmelos (AAM) and Aegeline (AG). 42 adult male wistar albino rats were separated into 7 groups, including Vehicle Control (VC); T2DM; T2DM + AAM 250 mg/kg; T2DM + AAM 500 mg/kg; T2DM + AG 20mg/kg; T2DM + AG 50 mg/kg and T2DM + AG 100 mg/kg. Experimental T2DM was created by a single dose of 40 mg/kg STZ injection intra-peritoneally along with 10% of fructose solution given orally for 30 days. Calculated dosages of AAM and AG were given with oral gavage for 30 days. Pancreas was harvested and processed. Slides were stained using hematoxylin and eosin (H&amp;E) stains. Insulin expressing beta-cells was analyzed using immunohistochemistry. Fructose fed, STZ induced rats showed degenerative expressions in beta-cells. In STZ treated rats, AG reduced the blood glucose concentration and serum insulin levels at the maximum functional dose compared to AAM. The immunohistochemical information suggests that the AG at 100 mg/kg dose has the capability of making the dormant cells to reproduce to restore the lost cells of islets of Langerhans.

Green Synthesis of Photocatalytic TiO2 Nanoparticles for Potential Application in Photochemical Degradation of Ornidazole

The present paper reports the green synthesis of TiO2 NPs to evaluate its photocatalytic activity. In this synthesis we use Aegle marmelos leaf extract as reducing and stabilizing agent. The TiO2 nanoparticles were characterized by using various advance analytical tools. The FTIR analysis revels the presence of different functional group present on the surface of the fabricated nanoparticles. The result of SEM analysis indicated that the prepared nanoparticles were almost spherical in nature and particles are in agglomerated state with bunch size less than 150 nm. The X-ray analysis of our synthesized nanoparticles clearly indicated that the particles are exit in anatase phase. Photocatalytic degradation studies of our nanoparticles were also evaluated against the most common nitroimidazole derivative drug ornidazole (ODZ) and the result of this study provide a good alternative path to remove the ODZ contaminants from waste water. The photocatalytic degradation of selected drug follow pseudo-first order reaction.

Anti-diabetic and nephrotoxicity effect of Aegle marmelos leaf on alloxan-induced diabetic rat

Aegle marmelos is widely found in india. The leaves, roots, fruits, bark, and seeds are extensively used in Ayurveda. . A. marmelos is mainly used for the treatment of diabetes mellitus. Alloxan (150 mg/kg b.w) was used in the Wistar rats for making the diabetic model. The oral administration of leaf extract of Aegle marmelos ( 200 and 400 mg/ kg b.w) was given for four weeks. The effect of ethanolic leaf extract of Aegle marmelos leaf extract on serum blood glucose as well as kidney function test [urea, uric acid, albumin, protein, and creatinine] were measured in the alloxan-induced diabetic rats. In the acute toxicity study, the ethanolic leaf extract of Aegle marmelos leaf was non-toxic at 2000 mg/kg in rats. The level of albumin and protein had significantly increased along with the serum glucose and urea, uric acid levels when they were observed and reduced in diabetic rats treated with both doses of ethanol leaf extract of Aegle marmelos as compared to diabetic group. Histopathological studies were revealed toward normal. Ethanolic extract of Aegle marmelos leaf possesses the significant anti-diabetic and rejuvenating capability of kidney function tests.

Instant synthesis of silver particles on silk fibres: Characterization and antimicrobial study

Silk fibers utilized as promising templates to produce antimicrobial composites and organic-inorganic advanced materials. The process involves higher incubation times and more preparation steps with Ag precursors. To overcome such procedural bottlenecks, this study aims at a quick and eco-friendly approach to deposit silver nanoparticles on degummed silk fibers for antibacterial applications. Degummed fibers with a simple pre-treatment step with Aegle marmelos leaf extract solutions resulted in rapid nanosilver deposition. Results present that the formed nanoparticles (~70 nm) are optically active, crystalline structures and deposited uniformly on the silk surface. Furthermore, they are antibacterial when tested against Escherichia coli bacterium.

Effects of &amp;lt;i&amp;gt;Aegle marmelos&amp;lt;/i&amp;gt; (L.) Methanolic Leaf Extracts on Cardiovascular Parameters in Diabetic Rats

Aegle marmelos (L.) Correa is a widely found plant in India as well as in South Asia. For more than several centuries it is being widely used for its medicinal properties. The objective of the study was to evaluate the cardiovascular changes in alloxan induced diabetic rats treated with methanolic leaf extracts of Aegle marmelos. 5 treatment groups (namely control, diseased, low dose (100mg/kg), medium dose (250mg/kg) and high dose (500mg/kg) of methanolic leaf extracts were used in the study. The cardiovasculareffects were evaluated by the determination of Very-Low-Density Lipoprotein (VLDL), Serum Sialic Acid, Glutathione Peroxidase, Serum Catalase, Ascorbic acid, Sodium, Potassium, and Chloride levels. High dose treatment group showed significant decrease in Very-Low-Density Lipoprotein (VLDL), Serum Sialic Acid, Glutathione Peroxidase, Serum Catalase, Ascorbic acid, Sodium, Potassium, and Chloride levels when compared with the diseased treatment groups. Though Low and medium dose treated animals showed insignificant decrease in these cardiovascular parameters when compared with high dose treatment group as well as diseased group. The effects of high dose treatment on cardiovascular parameters were very significant as that of control group. Through the cardiovascular parameters it is evident that the highdose of methonolic leaf extract of Aegle marmelos can be used the treatment of diabetes and its cardiovascular complications.

Mineralization of carcinogenic anthracene and phenanthrene by sunlight active bimetallic oxides nanocomposites

Polycyclic aromatic hydrocarbons (PAHs) are causing environmental concerns due to their persistent nature and carcinogenicity. Hence, their removal through advanced nanomaterials with characteristics of low-cost and high efficiency is essential. In view of this, bimetallic oxides (BMOs) nanocomposites of NiO-ZnO, ZnCo2O4, MnCo2O4 and CoFe2O4 were synthesized via green route using leaf extract of Aegle marmelos. Subsequently, these BMOs were investigated for photocatalytic removal of selected PAHs like anthracene (ANTH) and phenanthrene (PHEN) from water. Nanospheres of NiO-ZnO, ZnCo2O4, and CoFe2O4 and nanosheets of MnCo2O4 with particle size range of 10–30 nm were confirmed by transmission electron microscopy. At neutral pH, nanocomposites showed excellent ability in degrading 2 mg L−1 of PAHs (ANTH: 98%; PHEN: 93%) within 12 h under the exposure of sunlight. Among the synthesized BMOs, NiO-ZnO was found best followed by ZnCo2O4, MnCo2O4 and CoFe2O4. This fact is attributed to the highest surface area (129 m2 g−1) and particles stability (zeta potential: −30 eV) of NiO-ZnO. Photodegradation of PAHs by nanocomposites followed first order kinetics and fitted in Langmuir model for adsorption. Higher degradation under sunlight and lower removal efficiency with scavenger confirmed the photodegradation activity of nanocomposites. Overall, reusable (n = 10) nanocomposites with no loss of activity have high photocatalytic potential in the removal of carcinogenic PAHs.

Evaluation of antiulcerogenic activity of combination of Aloe vera and Aegle marmelos in indomethacin induced gastric ulcers

Background: Most important adverse effect of NSAID is peptic ulceration. Even though H2 blockers and proton pump inhibitors are effective in preventing NSAID associated peptic ulceration, they are not without side effects. Hence there is a need for drugs which are effective in preventing NSAID induced peptic ulcer without producing side effects. Two plant products Aloe vera leaf extract and Aegle marmalos leaves are commonly used in Indian traditional medicine for treatment of peptic ulcers. Hence this study is undertaken to assess the antiulcerogenic potential of combination of these two drugs in comparison with ranitidine in preventing NSAID induced peptic ulcers.Methods: 18 albino rats were divided into 3 groups of 6 rats each. Group A: received ulcerogen only. Group B: pretreated with ranitidine before exposing to ulcerogen. Group C: pretreated with combination of Aloe vera and Aegle marmelos before exposing to ulcerogen. Two doses of indomethacin were administered at an interval of 15 hrs. Animals were sacrificed 6 hrs after the second dose of Indomethacin. Number of ulcers was noted, and ulcer index was calculated.Results: There was significant reduction in total score, mean score and ulcer index in ranitidine pretreated group and test compound group as compared to control group. Even though the total score and ulcer index in test group were lesser as compared to standard control group, it was not statistically significant.Conclusions: Combination of Aloe vera leaf extract and Aegle marmelos leaf extract produced very significant protection against indomethacin induced gastric ulcer.