Abstract
Background: Aegle marmelos commonly known as Bael is a herbal plant. Itis from a family called Rutaceae. It has many medicinal uses: anti-diarrheal, anti- microbial, anti-viral, anti-cancer, chemo-preventive, Ulcer healing and many others.
 Aim: To study the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells.
 Objective: The present study investigated the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells.
 Materials and Methods: DMSO and MTT chemicals were purchased from Sigma chemical Pvt Ltd. Trypsin EDTA, FBS, RPMI 1640 medium and PBS, Real time PCR kit was purchased from Canada. Human melanoma cell line (A375) was purchased from NCCS, Pune, India.
 Results: The data was analysed statistically by ANOVA and Duncan’s multiple range test with a computer based software (Graph Pad Prism version 5). The percentage of cell viability decreases with the increase in dosage of Aegle marmelos leaf extract. 
 Conclusion: The study concluded that Aegle marmelos hydro ethanolic leaf extract a novel and innovative herbal drug has a significant effect on the expression of antiapoptotic markers in human melanoma cell lines.
Highlights
Aegle marmelos (L.) Correa (A.marmelos) is commonly called “Bael”
Trypsin-EDTA, fetal bovine serum (FBS), antibiotics-antimycotics, RPMI 1640 medium and phosphate buffered saline (PBS) were purchased from Gibco, Cell viability was assayed employing a modified colorimetric technique that supported the power of live cells to convert MTT, a tetrazolium compound into purple formazan crystals by mitochondrial reductases (Mosmann, 1983)
Novel property of extracts of Aegle marmelos, medicinal plants which are of great use with both roots,leaves and fruits in various medicinal ways [28]
Summary
Aegle marmelos (L.) Correa (A.marmelos) is commonly called “Bael” It is a kind of tree from the family “Rutaceae” which was widely used in as Indian traditional medicinal plant because of its various medicinal properties [1]. In the last 20 years, plant endophytes have gained more attention towards themselves because they act as a source of secondary metabolites with a new chemical skeleton [5,7]. They are very well known for their properties of anti-proliferative, antipyretic, anti-oxidant, [8] antidiarrheal, anti-cancer [9-10]. Objective: The present study investigated the effect of Aegle marmelos hydroethanolic leaf extract on expression of antiapoptotic markers in human melanoma cells. Materials and Methods: DMSO and MTT chemicals were purchased from Sigma chemical
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