Phytofabrication of Silver Nanoparticles using aqueous leaf extract of Aegle marmelos and its Characterization

Abstract

Nanotechnology has proved a boon as it brings a revolutionary change in the world with the tremendous advancements in the field of science such as engineering, biotechnology, analytical chemistry, and agriculture. The synthesis of the metal nanoparticle is a developing area of exploration in present-day material science and innovation. Among the techniques involved in the synthesis of metallic nanoparticles, the biological methods or the green synthesis are reliable due to its cost-effective, environment friendly, and use of non-harmful materials in the processing. Therefore, the current work is focused on the biosynthesis of silver nanoparticles with the leaf extract of holy plant Aegle marmelos by utilizing 1mM silver nitrate solution. From the bio-reduction reaction mixture, the synthesis of silver nanoparticles was studied by UV-Visible spectrophotometer at the range of 200 nm to 800nm and discovered maximum absorbance at 460nm. Further, the SNPs were analyzed for the protein or other organic groups by Fourier Transformed Infrared spectrophotometer (FTIR). The dried silver nanoparticles were further characterized by utilizing a scanning electron microscope (SEM) to observe the actual size, shape, and distribution of particles. This result showed the actual size of the bioengineered silver nanoparticles ranges from 25-30nm. The EDX result showed a peak of Ag that confirmed its presence in the suspension. The main objective of this study is to develop a fast, environmentally friendly, and advantageous technique for the synthesis of silver nanoparticles. By standardizing the biosynthesis techniques, silver nanoparticles can be synthesized in bulk with a safe environment and it may be utilized for different clinical applications including control of pathogenic microorganisms and so on.