Objective To explore the application of One System Two comprehensive training method in practical teaching of Basic Nursing Technology. Methods A total of 223 specialist nursing students were divided into the experimental group(110 cases) and the control group(113 cases). The control group adopted the original teaching method in the training course of Basic Nursing Technology.The experimental group introduced the comprehensive training method of One System Two on the basis of the original teaching method.At the end of one year, the score of operation results and critical thinking ability of the two groups were compared and analyzed. Results The scores of operating performance of Basic Nursing Technology and critical thinking skills of the experimental group were higher than those of the control group, and the differences were statistically significant(P<0.05). Conclusion The introduction of One System Two Methods comprehensive training method into the training course of Basic Nursing Technology is helpful to improve the technical operation level and critical thinking ability of nursing students in junior college. Key words: Basic nursing; Skills operation; Critical thinking skills