The polyvinyl butyral (PVB) laminated glass (LG) structures are widely used in architectural and automotive sectors. Determination of the interfacial fracture energy (i.e. G) between the PVB layer and glass is one of the most vital procedures for ensuring the structural safety and fracture resistance of these kinds of components. The present study investigates the adhesion characteristics between glass and PVB interface in a LG composite structure. A new simple test fixture is introduced to measure the mixed-mode fracture energy from pure mode I (i.e. GI) to pure mode II (i.e. GII). By making use of the concept of Equivalent material Concept (EMC), linear elastic fracture mechanics (LEFM) formulations together with finite element (FE) analysis can be used to compute the compliance parameter of the system and pure modes I/II fracture energies. Besides, the interaction of cohesive forces between the PVB layer and glass are simulated by using a FE method based on a bi-linear cohesive zone model (CZM) technique. In this regard, the cohesive law parameters are computed for mode I and mode II in an iterative process. Then, the obtained pure mode cohesive law parameters are utilized to predict the mixed-mode load–displacement diagrams. The obtained results demonstrate the satisfactory accuracy of the cohesive law parameters and fracture energies in predicting the mixed-mode loading results across four different loading rates (i.e. LR = 2, 5, 10, and 15 mm/min).