Hydrodynamic Shear Research Articles

Hierarchical membrane integration of shear stress-resistant nanoparticles and biomimetic micropatterns for ultrahigh and durable biofouling resistance

• Anti-biofouling pattern and biocidal nanoparticles were hierarchically integrated onto membranes. • Langmuir-Schaefer technique and nonsolvent-induced phase separation were rationally combined. • Silver nanoparticle integration is highly resistant to hydrodynamic shear stress (30 cm/s). • The resultant membrane enhanced biofouling resistance by 100% compared to the pristine counterpart. Herein, we report a rational strategy for fabricating dual-functional microfiltration (MF) membranes hierarchically integrated with micropatterns and Ag nanoparticles (NPs) for ultrahigh and durable fouling resistance. To harness hydrodynamic force and chemical oxidation for an enhanced anti-biofouling ability, a monolayer of Ag NPs was transferred onto negative patterns of polyurethane acrylate molds via the Langmuir-Schaefer technique. Then, they were integrated onto the biomimetic micropattern of porous polyacrylonitrile (PAN) membranes through nonsolvent-induced phase separation. Without the use of chemical linkers, our method achieved a stable and partial embedment of Ag NPs onto the porous PAN network and exhibited a negligible loss of Ag NPs even after exposure to high shear stress. Compared with the same micropatterned MF membranes without Ag NPs and the bare MF membranes, the resultant MF membrane significantly suppressed the decrease in water flux for the microbial solution by over 40% and 100%, respectively, owing to the dual anti-bacterial activities of the biocidal property of Ag NPs and the hydrodynamic repulsion of the micropatterns. Our hierarchical fabrication method can be applied to various biocidal NPs for ultrahigh biofouling-resistant porous membranes.

Alginate Core-Shell Capsules for 3D Cultivation of Adipose-Derived Mesenchymal Stem Cells.

Mesenchymal stem cells (MSCs) are primary candidates in tissue engineering and stem cell therapies due to their intriguing regenerative and immunomodulatory potential. Their ability to self-assemble into three-dimensional (3D) aggregates further improves some of their therapeutic properties, e.g., differentiation potential, secretion of cytokines, and homing capacity after administration. However, high hydrodynamic shear forces and the resulting mechanical stresses within commercially available dynamic cultivation systems can decrease their regenerative properties. Cells embedded within a polymer matrix, however, lack cell-to-cell interactions found in their physiological environment. Here, we present a “semi scaffold-free” approach to protect the cells from high shear forces by a physical barrier, but still allow formation of a 3D structure with in vivo-like cell-to-cell contacts. We highlight a relatively simple method to create core–shell capsules by inverse gelation. The capsules consist of an outer barrier made from sodium alginate, which allows for nutrient and waste diffusion and an inner compartment for direct cell-cell interactions. Next to capsule characterization, a harvesting procedure was established and viability and proliferation of human adipose-derived MSCs were investigated. In the future, this encapsulation and cultivation technique might be used for MSC-expansion in scalable dynamic bioreactor systems, facilitating downstream procedures, such as cell harvest and differentiation into mature tissue grafts.

Analytical solutions for nonionic and ionic diffusio-osmotic transport at soft and porous interfaces

In this paper, we develop one of the first models for closed-form fully analytical solutions for describing the nonionic and ionic diffusio-osmotic (DOS) transport at interfaces grafted with a soft and porous polymeric film in the presence of a neutral solute concentration gradient (for nonionic diffusio-osmosis) and a salt concentration gradient (for ionic diffusio-osmosis). The nonionic DOS velocity depends on this solute concentration gradient and the drag force from the polymeric film. The ionic DOS transport is characterized by the diffusio-osmotically induced electric field and the diffusio-osmotically induced velocity field. This induced electric field is primarily dictated by the conduction of the mobile ion imbalance present within the electric double layer, induced at the charged solid, in the presence of the applied salt concentration gradient. The DOS velocity, on the other hand, is driven by a combination of the induced pressure gradient and an induced electro-osmotic body force (triggered by this induced electric field) and is opposed by the drag from the polymer layer. The result is a velocity field whose magnitude increases rapidly at near wall locations, decreases away from the wall, and depending on the salt concentration, may or may not increase outside the polymeric layer. This unique velocity profile ensures the presence of significant hydrodynamic shear stress across a wide zone extending from the wall in a non-confined fluidic system: This will ensure that finite-sized species (e.g., biological cells) can be conveniently made to access locations of large hydrodynamic stresses for a myriad of engineering and biological applications.

Systemic sterile induced-co-expression of IL-12 and IL-18 drive IFN-γ-dependent activation of microglia and recruitment of MHC-II-expressing inflammatory monocytes into the brain

The development of neuroinflammation, as well as the progression of several neurodegenerative diseases, has been associated with the activation and mobilization of the peripheral immune system due to systemic inflammation. However, the mechanism by which this occurs remains unclear. Here, we addressed the effect of systemic sterile induced-co-expression of IL-12 and IL-18, in the establishment of a novel cytokine-mediated model of neuroinflammation. Following peripheral hydrodynamic shear of IL-12 plus IL-18 cDNAs in C57BL/6 mice, we induced systemic and persistent level of IL-12, which in turn promoted the elevation of circulating pro-inflammatory cytokines TNF-α and IFN-γ, accompanied with splenomegaly. Moreover, even though we identified an increased gene expression of both TNF-α and IFN-γ in the brain, we observed that only IFN-γ, but not TNF-α signaling through its type I receptor, was required to induce both the trafficking of leukocytes from the periphery toward the brain and upregulate MHC-II in microglia and inflammatory monocytes. Therefore, only TNF-α was shown to be dispensable, revealing an IFN-γ-dependent activation of microglia and recruitment of leukocytes, particularly of highly activated inflammatory monocytes. Taken together, our results argue for a systemic cytokine-mediated establishment and development of neuroinflammation, having identified IFN-γ as a potential target for immunomodulation.

New Insights from Locally Resolved Hydrodynamics in Stirred Cell Culture Reactors

The link between hydrodynamics and biological process behavior of antibody-producing mammalian cell cultures is still not fully understood. Common methods to describe dependencies refer mostly to averaged hydrodynamic parameters obtained for individual cultivation systems. In this study, cellular effects and locally resolved hydrodynamics were investigated for impellers with different spatial hydrodynamics. Therefore, the hydrodynamics, mainly flow velocity, shear rate and power input, in a single- and a three-impeller bioreactor setup were analyzed by means of CFD simulations, and cultivation experiments with antibody-producing Chinese hamster ovary (CHO) cells were performed at various agitation rates in both reactor setups. Within the three-impeller bioreactor setup, cells could be cultivated successfully at much higher agitation rates as in the single-impeller bioreactor, probably due to a more uniform flow pattern. It could be shown that this different behavior cannot be linked to parameters commonly used to describe shear effects on cells such as the mean energy dissipation rate or the Kolmogorov length scale, even if this concept is extended by locally resolved hydrodynamic parameters. Alternatively, the hydrodynamic heterogeneity was statistically quantified by means of variance coefficients of the hydrodynamic parameters fluid velocity, shear rate, and energy dissipation rate. The calculated variance coefficients of all hydrodynamic parameters were higher in the setup with three impellers than in the single impeller setup, which might explain the rather stable process behavior in multiple impeller systems due to the reduced hydrodynamic heterogeneity. Such comprehensive insights lead to a deeper understanding of the bioprocess.

Обоснование математической модели многослойного сдвигового течения бетонной смеси по наклонной поверхности откоса канала

In assessing the hydrodynamic parameters of concrete mixtures placed in monolithic channel linings, the most important point is the development of mathematical models that describe the processes of concrete mixture slippage, taking into account the dynamics of speed reduction and increase in the viscosity of the concrete mixture over time. The main regularities of the hydrodynamic multilayer shear flow of a concrete mixture on the surface of a flat channel slope, where a multilayer plane-parallel viscous flow moves under the action of gravity, are investigated. The boundary value problem of a two-layer flow of a concrete mixture is solved, a system of two layers of viscous incompressible liquids is considered with the properties of each, respectively, the thickness of the bottom and upper layers, taking into account the sliding velocity of particles of a viscous liquid located one above the other. The obtained analytical solution of the problem in the stationary regime of a two-layer shear flow of two different immiscible viscous fluids with different properties makes it possible to formulate the main laws of such flows and describe the processes of sliding of a multilayer concrete mixture, taking into account the dynamics of speed reduction and increase in the viscosity of the concrete mixture over time. A mathematical model has been developed where the dynamic and kinematic structures of a multilayer shear flow in both viscous layers are presented separately.

Development of a Multi-target Protein Biomarker Assay for Circulating Tumor Cells.

We report a highly sensitive and selective CNT-switch liquid biopsy platform that detects and quantifies protein biomarker expressions from circulating tumor cells in blood for early detection of metastatic breast cancer and its relapse. This platform first isolates and enriches more than 99% of tumor cells with an off-chip micro-size membrane filtration technique and then conducts on-chip detection of the membrane and internal protein biomarkers of the tumor cells with high sensitivity and selectivity. High sensitivity is achieved with complete association of the antibody-antigen-antibody (Ab-Ag-Ab) complex by precisely and rapidly assembling carbon nanotubes (CNTs) across two parallel electrodes via sequential DC electrophoresis and dielectrophoresis (DEP) deposition. Each bridged CNT acts as a switch that connects the electrodes and closes the circuit to generate an electrical signal. The high selectivity is achieved with a critical hydrodynamic shear rate that irreversibly removes non-target linkers of the aligned CNTs. At present, we are able to detect the protein biomarkers from 5 spiked breast cancer tumor cells of different types within 7.5ml of human blood samples. This demonstrates the potential of this platform as an inexpensive and noninvasive alternative to MRI scans and tissue biopsies currently used to detect early metastatic breast cancer and its relapse.

Shear-induced migration of confined flexible fibers.

We report an experimental study of the shear-induced migration of flexible fibers in suspensions confined between two parallel plates. Non-Brownian fiber suspensions are imaged in a rheo-microscopy setup, where the top and the bottom plates counter-rotate and create a Couette flow. Initially, the fibers are near the bottom plate due to sedimentation. Under shear, the fibers move with the flow and migrate towards the center plane between the two walls. Statistical properties of the fibers, such as the mean values of the positions, orientations, and end-to-end lengths of the fibers, are used to characterize the behaviors of the fibers. A dimensionless parameter Λeff, which compares the hydrodynamic shear stress and the fiber stiffness, is used to analyze the effective flexibility of the fibers. The observations show that the fibers that are more likely to bend exhibit faster migration. As Λeff increases (softer fibers and stronger shear stresses), the fibers tend to align in the flow direction and the motions of the fibers transition from tumbling and rolling to bending. The bending fibers drift away from the walls to the center plane. Further increasing Λeff leads to more coiled fiber shapes, and the bending is more frequent and with larger magnitudes, which leads to more rapid migration towards the center. Different behaviors of the fibers are quantified with Λeff, and the structures and the dynamics of the fibers are correlated with the migration.

Hydrodynamically driven self-assembly of lignin bowls and spheres by line-type micro-mixer

We report a strategy to continuously prepare lignin bowls and spheres using hydrodynamic flow in a simple line-type micro-mixer device. The kinetics of the non-equilibrium self-assembly of lignin was controlled by hydrodynamic shearing forces. It indicates that the convective-diffusive mixing and mass transfer of lignin macromolecules at the boundary of THF and water lamellar fluids are critical for the self-assembly of well-defined lignin nanoparticles. The shearing force of water on THF decreased with the decreasing of flow rate ratio (FRR) of water to THF from 30 to 7.5, resulting in an increase in lignin bowls yield from 20.9% to 54.7%. Furthermore, the diameter of lignin nanoparticles can be controlled from 100 to 550 nm by changing the flow rate. In addition, the diffusion coefficient of solvent also plays an imperative part in the self-assembly process. Under the same hydrodynamic conditions, solvents with higher diffusion coefficients generate more nanospheres.

A TECHNIQUE OF PANELS CUTTING FOR MODIFICATION OF HULL GEOMETRY HYDRODYNAMIC MODELS

A panel cutting technique is developed for automatic modification of an initial mesh of a ship hull used for hydrodynamic computations leading to improved meshes for the prediction of wave induced vertical load effects. The technique can provide a model with divided panels in any defined position regardless of the initial discretization of the body. The applications of the provided technique include panel distinction and division in predetermined positions, generation of finer mesh based on the initial coarser model of meshes and improvement of vertical load prediction in predetermined positions. The method is applied for case studies of a barge, shuttle tanker and frigate to depict various applications. Finally, the hydrostatic and hydrodynamic vertical shear forces are calculated for two models of initial and modified panels of well-known frigate 5415. The results are compared for the sections alongside the ship and accuracy of load integration is shown for predetermined sections.

Slippery liquid-infused microphase separation surface enables highly robust anti-fouling, anti-corrosion, anti-icing and anti-scaling coating on diverse substrates

Slippery liquid-infused porous surface (SLIPS) strategy has demonstrated great potential in the field of both biomedical and industrial applications. However, the fabrication of large-area SLIPS with a combined competence of strong bonding to diverse substrates as well as robust oil-locking ability especially those with special shape and structure remains highly challenging. Herein, SLIPS was facilely constructed on various substrates and shapes in bulk via a step-by-step deposition strategy. Collective results demonstrated that the facile and feasible formation of SLIPS coating implemented the combination of multiple excellent properties, including anti-fouling, anti-corrosion, anti-icing, anti-scaling, and readily self-healing. In particular, the spontaneous microphase separation on the surface induced by the prominent polar differences between the superhydrophobic fluorinated segments and hydrophilic hydroxyl and amino groups enabled ultra-stable oil-locking ability, ensuring high robustness under conditions of blood flow, in vivo transplantation, hydrodynamic shear stress even accompanied with boiling and ultrasound, and mechanical wear. Therefore, such desirable properties will endow this SLIPS coating strategy promising practical applications in surgical equipment, medical implants, marine and industrial facilities.

Exposure of Von Willebrand Factor Cleavage Site in A1A2A3-Fragment under Extreme Hydrodynamic Shear.

Von Willebrand Factor (vWf) is a giant multimeric extracellular blood plasma involved in hemostasis. In this work we present multi-scale simulations of its three-domains fragment A1A2A3. These three domains are essential for the functional regulation of vWf. Namely the A2 domain hosts the site where the protease ADAMTS13 cleavages the multimeric vWf allowing for its length control that prevents thrombotic conditions. The exposure of the cleavage site follows the elongation/unfolding of the domain that is caused by an increased shear stress in blood. By deploying Lattice Boltzmann molecular dynamics simulations based on the OPEP coarse-grained model for proteins, we investigated at molecular level the unfolding of the A2 domain under the action of a perturbing shear flow. We described the structural steps of this unfolding that mainly concerns the -strand structures of the domain, and we compared the process occurring under shear with that produced by the action of a directional pulling force, a typical condition of single molecule experiments. We observe, that under the action of shear flow, the competition among the elongational and rotational components of the fluid field leads to a complex behaviour of the domain, where elongated structures can be followed by partially collapsed melted globule structures with a very different degree of exposure of the cleavage site. Our simulations pose the base for the development of a multi-scale in-silico description of vWf dynamics and functionality in physiological conditions, including high resolution details for molecular relevant events, e.g., the binding to platelets and collagen during coagulation or thrombosis.

Electrical Propulsion and Cargo Transport of Microbowl Shaped Janus Particles.

Herein the effective electrical propulsion, cargo trapping, and transport capabilities of microbowl-shaped Janus particles (JPs) are demonstrated and evaluated. These active JPs are made by deposition of Au and Ti layers onto sacrificial spherical polystyrene particles, followed by oxidation of the Ti to TiO2 . In contrast to the commonly studied spherical JP, the dual broken symmetry of both geometrical and electrical properties of the microbowl renders a strong dependence of its mobility and cargo loading on the order of the layering of Au and TiO2 . Specifically, an opposite direction of motion is obtained for interchanged layers of Au and TiO2 , using only electrical propulsion as the sole mechanism of motion. The concave side of the microbowl exhibits a negative dielectrophoretic trap of large size wherein trapped cargo is protected from hydrodynamic shearing, leading to an enhanced cargo loading capacity compared to that obtained using common spherical JP. Such enhanced cargo capability of the microbowl along with the ease of engineering it by interchanging the order of the layers are very attractive for future in vitro biological and biomedical applications.

Effect of hydrodynamic breakage on floc evolution and turbidity reduction in flocculation and sedimentation processes

Abstract Coagulation and sedimentation process is one of the most popular processes in drinking water treatment. Hydrodynamic breakage has a significant impact on the evolution of floc characteristics and the efficiency of turbidity removal. In this work, the effects of hydrodynamic breakage on floc size, fractal dimension, and floc morphology were investigated with an in-situ recognition system. The experiments were conducted in a continuous flocculation and sedimentation reactor equipped with perforated plates to provide different hydrodynamic breakage conditions. The experimental results indicated that the hydrodynamic conditions significantly influenced the floc destabilization and restructuring processes. A low hydrodynamic shear force provided by P1 led to the increase of both bigger sized flocs but accompanied with small particles (0–10 μm). Excessive velocity gradient provided by P3 produced smaller and looser flocs. An appropriate velocity gradient (i.e., the flow velocity through the perforated plate P2 at 18.9 × 10−3m s−1) was conducive for the formation of larger and more compact structures, with higher average floc size and fractal dimension. This flocculation condition in turn resulted in effective improvements in the turbidity removal efficiency. Floc evolution models were described based on the mechanism of the breakage and restructuring process.

Application of Coagulation-Membrane Rotation to Improve Ultrafiltration Performance in Drinking Water Treatment.

The combination of conventional and advanced water treatment is now widely used in drinking water treatment. However, membrane fouling is still the main obstacle to extend its application. In this study, the impact of the combination of coagulation and ultrafiltration (UF) membrane rotation on both fouling control and organic removal of macro (sodium alginate, SA) and micro organic matters (tannic acid, TA) was studied comprehensively to evaluate its applicability in drinking water treatment. The results indicated that membrane rotation could generate shear stress and vortex, thus effectively reducing membrane fouling of both SA and TA solutions, especially for macro SA organics. With additional coagulation, the membrane fouling could be further reduced through the aggregation of mediate and macro organic substances into flocs and elimination by membrane retention. For example, with the membrane rotation speed of 60 r/min, the permeate flux increased by 90% and the organic removal by 35% in SA solution, with 40 mg/L coagulant dosage, with an additional 70% increase of flux and 5% increment of organic removal to 80% obtained. However, too much shear stress could intensify the potential of fiber breakage at the potting, destroying the flocs and resulting in the reduction of permeate flux and deterioration of effluent quality. Finally, the combination of coagulation and membrane rotation would lead to the shaking of the cake layer, which is beneficial for fouling mitigation and prolongation of membrane filtration lifetime. This study provides useful information on applying the combined process of conventional coagulation and the hydrodynamic shear force for drinking water treatment, which can be further explored in the future.

Diffusion of Lipid Nanovesicles Bound to a Lipid Membrane Is Associated with the Partial-Slip Boundary Condition.

During diffusion of nanoparticles bound to a cellular membrane by ligand–receptor pairs, the distance to the laterally mobile interface is sufficiently short for their motion to depend not only on the membrane-mediated diffusivity of the tethers but also in a not yet fully understood manner on nanoparticle size and interfacial hydrodynamics. By quantifying diffusivity, velocity, and size of individual membrane-bound liposomes subjected to a hydrodynamic shear flow, we have successfully separated the diffusivity contributions from particle size and number of tethers. The obtained diffusion-size relations for synthetic and extracellular lipid vesicles are not well-described by the conventional no-slip boundary condition, suggesting partial slip as well as a significant diffusivity dependence on the distance to the lipid bilayer. These insights, extending the understanding of diffusion of biological nanoparticles at lipid bilayers, are of relevance for processes such as cellular uptake of viruses and lipid nanoparticles or labeling of cell-membrane-residing molecules.

Critical Analysis of Methods Adopted for Evaluation of Mixing Efficiency in an Anaerobic Digester

The effect of slurry mixing in an anaerobic digester on biogas production was intensively studied in the last few years. This subject is still debatable due to fact that this process involves three phases, solid-gas-liquid, along with the involvement of microbes during biochemical reactions, which are highly vulnerable to changes in hydrodynamic shear stresses and mixing conditions. Moreover, the complexity in the direction of optimization of mixing magnifies due to the implication of both fluid mechanics and biochemical engineering to study the effect of mixing in anaerobic digestion (AD). The effect of mixing on AD is explored using recent literature and theoretical analysis, concentrating on the multi-phase and multi-scale aspects of AD. The tools and methods available to experimentally quantify the function of mixing on both the global and local scales are summarized in this study. The major challenge for mixing in an anaerobic digester is to minimize dead zones and maintain uniform distribution of viscosity and shear at low mixing intensities without disrupting the microbial flocs and syntrophic relationships between the bacteria during the AD process. This study is a critical analysis of various techniques and approaches adopted by researchers to evaluate the effectiveness of mixing regimes and mixing equipment. Most studies describe biogas production performance and hydrodynamic characteristics of the digesters separately, but the evaluation of mixing requires interdisciplinary experts, which include mechanical engineers, microbiologists and hydrodynamic experts. Through this review, the readers will be guided through intensive literature regarding agitation, the best possible way to scrutinize the agitation problems and the approach to answering the question “why is the optimization of mixing in an anaerobic digester still a debatable subject?”.

Hydrodynamic dispersion relations at finite coupling

By using holographic methods, the radii of convergence of the hydrodynamic shear and sound dispersion relations were previously computed in the mathcal{N} = 4 supersymmetric Yang-Mills theory at infinite ’t Hooft coupling and infinite number of colours. Here, we extend this analysis to the domain of large but finite ’t Hooft coupling. To leading order in the perturbative expansion, we find that the radii grow with increasing inverse coupling, contrary to naive expectations. However, when the equations of motion are solved using a qualitative non-perturbative resummation, the dependence on the coupling becomes piecewise continuous and the initial growth is followed by a decrease. The piecewise nature of the dependence is related to the dynamics of branch point singularities of the energy-momentum tensor finite-temperature two-point functions in the complex plane of spatial momentum squared. We repeat the study using the Einstein-Gauss-Bonnet gravity as a model where the equations can be solved fully non-perturbatively, and find the expected decrease of the radii of convergence with the effective inverse coupling which is also piecewise continuous. Finally, we provide arguments in favour of the non-perturbative approach and show that the presence of non-perturbative modes in the quasinormal spectrum can be indirectly inferred from the analysis of perturbative critical points.

Hydrodynamic shear dissipation and transmission in lipid bilayers

Vital biological processes, such as trafficking, sensing, and motility, are facilitated by cellular lipid membranes, which interact mechanically with surrounding fluids. Such lipid membranes are only a few nanometers thick and composed of a liquid crystalline structure known as the lipid bilayer. Here, we introduce an active, noncontact, two-point microrheology technique combining multiple optical tweezers probes with planar freestanding lipid bilayers accessible on both sides. We use the method to quantify both fluid slip close to the bilayer surface and transmission of fluid flow across the structure, and we use numerical simulations to determine the monolayer viscosity and the intermonolayer friction. We find that these physical properties are highly dependent on the molecular structure of the lipids in the bilayer. We compare ordered-phase with liquid disordered-phase lipid bilayers, and we find the ordered-phase bilayers to be 10 to 100 times more viscous but with 100 times less intermonolayer friction. When a local shear is applied by the optical tweezers, the ultralow intermonolayer friction results in full slip of the two leaflets relative to each other and as a consequence, no shear transmission across the membrane. Our study sheds light on the physical principles governing the transfer of shear forces by and through lipid membranes, which underpin cell behavior and homeostasis.

Motility Response to Hydrodynamic Stress During the Growth Cycle in Active Fluid Suspensions

Abstract In this work, we focus on the motility behavior of two model micro-organisms widely used in the study of active fluids: Chlamydomonas reinhardtii micro-alga and Synechocystis sp. cyanobacterium. Understanding the physiological responses of micro-organisms under variable environmental conditions is essential for bioreactor engineering. Yet, most of the previous studies focused on the observation of cellular motility regardless of the growth process. Here, we measure the motility of Chlamydomonas reinhardtii and Synechocystis sp. during their growth when subjected to different intensities of hydrodynamic shear stress. The results demonstrate a significant difference in the motility response of the two species against the applied hydrodynamic shear stress. Mechanical agitation appears to affect the motility of Chlamydomonas reinhardtii micro-algae by stimulating the growth process and increasing the magnitude of the cellular swimming velocity. This effect is described using an empirical model for the time variation of the motility. Synechocystis cells show a high endurance to the applied shear such that the global effect of agitation intensity on their motility is insignificant. However, it seems that the peak of the swimming velocity always occurs in the middle of exponential phase of growth.