Diffusion of Lipid Nanovesicles Bound to a Lipid Membrane Is Associated with the Partial-Slip Boundary Condition.

Erik Olsén,Adrián González,Paul Joyce,Silver Jõemetsa,Daniel Midtvedt,Fredrik Höök,Vladimir P Zhdanov

doi:10.1021/acs.nanolett.1c02092

Abstract

During diffusion of nanoparticles bound to a cellular membrane by ligand–receptor pairs, the distance to the laterally mobile interface is sufficiently short for their motion to depend not only on the membrane-mediated diffusivity of the tethers but also in a not yet fully understood manner on nanoparticle size and interfacial hydrodynamics. By quantifying diffusivity, velocity, and size of individual membrane-bound liposomes subjected to a hydrodynamic shear flow, we have successfully separated the diffusivity contributions from particle size and number of tethers. The obtained diffusion-size relations for synthetic and extracellular lipid vesicles are not well-described by the conventional no-slip boundary condition, suggesting partial slip as well as a significant diffusivity dependence on the distance to the lipid bilayer. These insights, extending the understanding of diffusion of biological nanoparticles at lipid bilayers, are of relevance for processes such as cellular uptake of viruses and lipid nanoparticles or labeling of cell-membrane-residing molecules.

Highlights

During diffusion of nanoparticles bound to a cellular membrane by ligand−receptor pairs, the distance to the laterally mobile interface is sufficiently short for their motion to depend on the membrane-mediated diffusivity of the tethers and in a not yet fully understood manner on nanoparticle size and interfacial hydrodynamics
In the case of nanoparticles near biological interfaces, nanoparticle diffusivity can be used to estimate both the nature of the interfacial interactions and nanoparticle size.[1−3] confined nanoparticle diffusion is significantly influenced by hydrodynamical boundary conditions in general and especially when the distance between the nanoparticle and an interface is shorter than the size of the particle,[1,4] which naturally occurs during the initial interaction between biological nanoparticles and cellular membranes.[5]
Since the hydrodynamics around hydrophilic interfaces often is well-described by the no-slip boundary condition,[10] this boundary condition is typically employed for biological interfaces, as they often consist of lipid bilayers with hydrophilic headgroups facing the surrounding fluid

Summary

Corresponding Authors

Silver Jõemetsa − Department of Physics, Chalmers University of Technology, SE-41296 Göteborg, Sweden; orcid.org/ 0000-0001-7957-8750. Adrián González − Department of Physics, Chalmers University of Technology, SE-41296 Göteborg, Sweden; orcid.org/0000-0002-0965-0822. Paul Joyce − Department of Physics, Chalmers University of Technology, SE-41296 Göteborg, Sweden; UniSA: Clinical and Health Sciences, University of South Australia, 5000 Adelaide, Australia; orcid.org/0000-0003-3619-7901. Zhdanov − Department of Physics, Chalmers University of Technology, SE-41296 Göteborg, Sweden; Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia; orcid.org/0000-0002-01678783. Daniel Midtvedt − Department of Physics, University of Gothenburg, SE-41296 Göteborg, Sweden; orcid.org/ 0000-0003-4132-4629.

■ ACKNOWLEDGMENTS

■ REFERENCES