Rules Of Green Chemistry Research Articles

Synthesis and investigation of photo-physical properties of fluorescent dyes obtained by the Knoevenagel condensation of mono-, di- or tri-formyl aromatic aldehydes and various CH-acid derivatives

Fluorescent dyes were constructed using the Knoevenagel condensation of the appropriate aldehydes and CH-acid derivatives. Aromatic aldehydes containing one, two or three formyl groups were used as substrates. Cyanoacetamide, ethyl cyanoacetate, benzoylacetonitrile and indan-1,3-dione play the role of CH-acid derivative. The appropriate aldehydes were obtained by the Duff formylation reaction. The dyes synthesis procedure was very simple. The condensation was carried out in water in the presence of NaOH, as the basic catalyst at room temperature, and the dyes were obtained in yields between 58-93%. The work-up procedure was very simple, and the products do not require any further purification. The great advantages of this synthetic procedure are its simplicity, cheapness, compliance with the rules of green chemistry and the fact that it efficiently leads to structurally complex fluorescent dyes. Twenty-three new compounds were obtained, eleven of which exhibited fluorescence in solution and four in the solid state. Taking into account the fluorescence quantum yield and the emission maximum value close to the NIR range, the dye constructed by condensation of indan-1,3-dione and methyl 3,5-diformyl-4-hydroxybenzoate meets the above-mentioned requirements (Φ=16%, λem= 660 nm) best. The dye with the highest value of Φ = 25% in solid is a derivative of cyanoacetamide and 3,4,5-trimethoxybenzaldehyde.

CaAl1.5Fe0.5O4/6%Ni-ZnO nanocomposites: Synthesis, characterization and effective photocatalyst for the photocatalytic degradation of methylene blue dye

This study focuses on synthesizing and analyzing properties of calcium aluminate photocatalytic nanoparticles using the microwave (MW) combustion method by following the rules of green chemistry and investigates their effectiveness in the adsorption of methylene blue (MB) dye which is a crucial contaminant that enters water resources. The synthesized nanoparticles, including CaAl2O4 (CAO), CaAl1.5Fe0.5O4 (CAFO), 6 %Ni-ZnO (NZO), and CaAl1.5Fe0.5O4/6%Ni-ZnO (CAFONZO) composites, were characterized using various techniques. XRD analysis confirmed the formation of spinel structures in all samples. The crystallite size and lattice constants were determined, showing a reduction in Crystallite size of CAO from 45.98 nm to 37.55 nm after Fe3 + doping. Then, with the formation of CAFONZO Crystallite size 13.99 nm was obtained. FT-IR analysis revealed the chemical interactions between atoms in the spinel structures Nitrogen adsorption/desorption isotherms were used to determine the specific surface area of nanoparticles, pore volume, and pore size. Specific surface area of CAO, CAFO, and CAFONZO are 1.637, 1.745, and 9.903 (m2/g), respectively. SEM and TEM images provided insight into the morphology and size of the nanoparticles. According to TEM images, after Fe3 + doping, the size of particles decreased from 85 to 32 nm. UV-DRS was performed to determine the optical band gap energy of the photocatalysts. The calculated band gap values of pure CAO, CAFO, and NZO nanoparticles were 3.2 eV, 2.5 eV, and 2.7 eV, respectively. Mott-Schottky analysis indicated the type of semiconductors and the energy levels of the conduction and valence bands. Valence band (VB) values of CAFO and NZO are 2.86 eV and 2.03 eV, respectively. The photocatalytic activity of the nanoparticles was evaluated by degrading MB under visible light illumination. The adsorption studies were conducted by varying the dosage, pollutant concentration, and pH. The results showed that CAFONZO nanoparticles exhibited the highest photocatalytic efficiency, achieving 96 % degradation of MB within 120 min. The combined photocatalyst demonstrated improved efficiency compared to the individual components.

New thermal insulating polyurethane biofoams based on cherry seed oil

AbstractCherry fruits were used in clean technologies for preparation of hydroxyl components applied in polyurethane foams. Polyphenols from cherry fruits helped to obtain a stable suspension of nanosilver particles dispersed in diethylene glycol, while cherry seeds constituted a renewable source of oil, which was transesterified into biopolyol. Such biocomponents containing nanosilver particles were applied to obtain open cell heat insulating polyurethane foams resistant to a fungus strain Candida albicans. The influence of nanosilver concentration in diethylene glycol, reaction temperature, and catalyst concentration on the biopolyols and the properties of the final foams were subjected to a statistical analysis. It was found that the following process conditions were crucial in obtaining the most desired foams (in terms of their physical and mechanical properties): the catalyst content—0.225%, the concentration of DEG with nanoAg—25%, temperature—188 °C. Such an approach allowed us to obtain open cell polyurethane foams in line with Green Chemistry rules. Graphical abstract

Effect of various aging conditions and treatment methods on thermal degradation of coffee waste/epoxy composites

In composites containing lignocellulosic fillers, the chemical composition of the filler mostly determines the heat resistance. Based on the rules of green chemistry, new bio-based composites were developed from the waste of industrial crop product coffee (CW), and their thermal stability in different aging environments was examined by thermogravimetric analysis (TGA). Alkali (NaOH), microwave (MW), ultrasound (US), and alkali pretreatment followed by microwave (NaOH+MW) were applied to modify the CW. The effect of various applied treatment methods and environmental conditions such as UV radiation, water sorption, seawater, and hydrothermally agings on the thermal properties of the composites was investigated. Fourier-transform infrared spectroscopy (FTIR) and Field emission scanning electron microscopy (FE-SEM) were used in the characterization of composites. Among the environmental factors tested, seawater and hydrothermal aging affected the thermal resistance of the composites the most, while NaOH+MW affected it most among the processing methods. Although the thermal stability of unaged and UV-exposed MW-CW composites was found to be the highest, it was more affected by aging in seawater, pure water, and hydrothermal environments. As a result, MW was found to be more effective among the treatment methods in terms of thermal properties under UV conditions, confirming that the adoption of environmentally friendly methods and surface modification of the filler can provide superior composites.

Extraction and green template-free synthesis of high-value mesoporous γ-Al2O3 and SiO2 powders from coal gangue

The study successfully demonstrated an eco-friendly approach for synthesizing mesoporous alumina and mesoporous silica from coal gangue, in line with green chemistry rules. By employing natural breakdown and increased dissolution of coal gangue through specific chemical treatments, along with optimal leaching conditions, this study achieved a dissolution efficiency of over 98% for aluminum. This process, which includes leaching, separation, simultaneous precipitation, selective dissolution, gel formation, and calcination, produced mesoporous alumina and mesoporous silica without the need for templates. The results presented that the synthesized mesoporous alumina and silica had purities of 98.81% and 99.08%, respectively. Additionally, the synthesized mesoporous alumina and silica had specific surface areas of 340.15 and 392.85 m2/g, and average pore sizes of 7.27 and 3.25 nm, confirming the desirable physical properties of the synthesized γ-Al2O3 and SiO2. These products offer a sustainable solution to environmental issues caused by coal accumulation, with promising applications across various industries.

Panax ginseng root aqueous extract mediated biosynthesis of silver nanoparticles under ultrasound condition and investigation of the treatment of human lung adenocarcinoma with following the PI3K/AKT/mTOR signaling pathway

In the current study, ultrasound-assisted biosynthesis of silver nanoparticles was described according to green chemistry rules by the aqueous extract of root’s Panax ginseng. The biosynthesized Ag NPs were characterized using different techniques, like FE-SEM, UV–Vis, EDS, elemental mapping and TEM. The shape, morphology and elemental composition of the prepared silver nanoparticles were studied by the FE-SEM and EDS data. The TEM image shows the monodispersed and spherical nanoparticles of size ranging from 20 to 40 nm. Colorimetric DPPH tests were done to assess the antioxidant capacity of Ag NPs and the MTT method to investigate the cytotoxicity against PC14 lung adenocarcinoma cell line as well as the normal HUVEC cell linein vitro. The IC50 of the Ag NPs nanocomposite was 157 µg/mL against PC14 cancer cell line. The Ag NPs nanocomposite increased the LDH release, apoptosis and ROS levels and regulated the PI3K/AKT/mTOR signaling pathway in the PC14 cells.

Polyfluorinated alcohol as a reusable media promoted catalyst-free green synthesis of 1H-pyrazolo[1,2-b]phthalazine-5,10-dione derivatives

A green way to catalyst-free one-pot production of 1H-pyrazolo[1,2-b]phthalazine-5,10-dione derivatives is described. It uses the Knoevenagel-Michael cyclic condensation method and polyfluorinated alcohol as a reusable promoting medium. This method follows the rules of green chemistry. Carrying out the reactions takes much less time while obtaining products with outstanding yields. This eco-friendly method uses safe reactions, doesn't need to be separated with column chromatography, is easy to produce and work-up, doesn't need a catalyst, is affordable and simple, and is cost-effective. Moreover, green 2,2,2-trifluoroethanol (TFE) is very stable and can be used again for 4 times without any major changes or loss of effectiveness. This makes it very useful for protecting the environment during industrial activities.

Design, preparation and characterization of magnetic nanoparticles functionalized with chitosan/Schiff base and their use as a reusable nanocatalyst for the green synthesis of 1H-isochromenes under mild conditions.

In this study, a Schiff base complex magnetic nanocatalyst was designed and prepared. The structure of the Fe3O4@CS-SB-CaMgFe2O4 nanocatalyst was characterized using FT-IR spectroscopy, XRD, VSM, FE-SEM, EDX, elemental mapping, BET, and TGA techniques. The synthesis of 1H-isochromene compounds has attracted the attention of chemists due to their biological and medicinal properties. The 1H-isochromene derivatives were synthesized in the presence of the Fe3O4@CS-SB-CaMgFe2O4 nanocatalyst with excellent efficiency and short reaction time as well as according to the rules of green chemistry. This reaction was carried out using Fe3O4@CS-SB-CaMgFe2O4 as a catalyst to develop a simple method with low activation energy at room temperature under optimal conditions. This catalyst provides a promising route for the synthesis of 1H-isochromene multiple times through its recyclability without significant loss of catalytic activity. This nanocatalyst possesses several advantages, including cost-effectiveness, facile separation, environmental friendliness, and recyclability, for the efficient production of 1H-isochromenes. The obtained compounds were further analyzed using spectroscopic techniques, such as melting point, FT-IR, 1H NMR, and 13C NMR analyses, to confirm their structures. The spectra of the synthesized compounds were recorded and analyzed, and a plausible mechanism for their synthesis was proposed. The characterization results and structural elucidation provide valuable insights into the preparation of these compounds.

Preparation and formulation of Ag NPs for the anticancer activities and its protective effects on cardiac function, heart rate variability, and cardiac mitochondria in obese insulin-resistant rats

Panax ginseng as an ethnomedicinal plant has many therapeutic effects in the traditional medicine. This medicinal plant, having antioxidant substances, can protect body cells from various damages and prevent cancer. Also, folic acid in this plant helps the body to produce new cells and reduces the possibility of heart disease and colon cancer. In the recent years, the silver nanoparticles green-mediated by ethno medicinal plants have been used for the treatment of several kinds of cancers and heart disease. Now, we investigated the silver nanoparticles green-synthesized according to green chemistry rules using the aqueous extract of Panax ginseng leaf for the treatment of lung carcinoma and protective effects on cardiac function, heart rate variability, and cardiac mitochondria in obese insulin-resistant rats. The green-synthesized silver nanoparticles were characterized using different techniques such as FE-SEM, UV–Vis, XRD, and TEM. The FE-SEM and TEM results confirm spherical morphology for the nanoparticles with size of 12.11 to 43.33 nm. The IC50 of the silver nanoparticles was 311, 293, 375, and 250 against EKVX, HOP-62, A549 and NCI-H460, respectively. In the in vivo part of this study, after treating 12 weeks with high-fat-diet, then 28 days with Ag NPs (20 and 40 µg/kg/day, respectively), the metabolic parameters, heart rate variability (HRV), cardiac function, and cardiac mitochondrial function were determined. Silver nanoparticles at both concentrations significantly decreased the plasma insulin, total cholesterol, homeostasis model assessment index, and oxidative stress levels and restored the HRV, cardiac function, and cardiac mitochondrial function. We concluded that silver nanoparticles at both concentrations exerted cardioprotective effects against mitochondrial dysfunction and cardiac dysfunction in obese insulin-resistant rats.

Treatment of nerve cancer with the green synthesis of CuO NPs

With technological advances in molecular techniques and bioinformatics, many information has been gained that will help in the cancer early diagnosis. Studying and investigating agents of natural origin, such as plants nanocompounds is one of the most important research goals in the cancer treatment field. In the recent study, copper nanoparticles were synthesized according to green chemistry rules using the Boswellia thurifera aqueous extract. After doing the clinical trial studies, the recent nanoparticles may be used as an anti-carcinoma supplement in humans. The properties of copper nanoparticles against common human nerve carcinoma cell lines i.e. were determined. The green-synthesized copper nanoparticles were characterized using different techniques such as X-ray diffraction (XRD), energy dispersive X-ray (EDX), fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), and ultraviolet–visible spectroscopy (UV–Vis). The FE-SEM results confirm spherical morphology for the nanoparticles with size of 14.1 to 37.9 nm. The IC50 of the copper nanoparticles was 163 and 245 against S462 Human Peripheral Nerve Sheath Tumor and BL1391 cancer cells, respectively. Copper nanoparticles induce cell apoptosis, accompanied by the pro-apoptotic markers upregulation (cleaved caspase-8 and Bax) and anti-apoptotic marker downregulation, Bcl-2. Also, copper nanoparticles prevent colony formation compared to their matched control. More significantly, the molecular pathway analysis of copper nanoparticles-treated cells indicated that copper nanoparticles raises p53 expression, while preventing the expression of total and phosphorylated Signal Transducer and Activator Of Transcription 3 (STAT3) in cell lines, offering p53 and STAT3 are the main key players behind the biological events provoked by the extract in human cancer cells.

Nickel ferrite nanoparticles doped on hollow carbon microspheres as a novel reusable catalyst for synthesis of N-substituted pyrrole derivatives

Pyrroles are widely spread worldwide because of their critical applications, especially pharmacology. An expedition method for one-pot synthesis of N-substituted pyrrole derivatives has been presented by a reaction between 2,5-dimethoxytetrahydrofuran and various primary aromatic amines in the presence of NiFe2O4 anchored to modified carbon hollow microspheres (NiFe2O4@MCHMs) as a recoverable reactive catalyst. The Classon-Kass method has been used to synthesize the pyrroles in excellent yields and short reaction times in the same direction with green chemistry rules. This reaction was carried out by employing NiFe2O4@MCHMs as a catalyst to make a simple procedure with short activation energy in water as an accessible, non-toxic, and biodegradable solvent. This catalyst provides a promising pathway to synthesize N-substituted pyrroles several times in a row through the recyclability without remarkable loss of its catalytic activity. The NiFe2O4@MCHMs nanocatalyst was characterized by applying FT-IR, XRD, FE-SEM, TEM, EDS, BET, TGA, VSM, and elemental mapping techniques. Also, the synthesized N-substituted pyrrole derivatives were identified using melting point, FT-IR, and 1H NMR analyses.

Allium ampeloprasum leaf aqueous extract green-formulated Ag nanoparticles: Determination of anti-human lung cancer and antioxidant effects

Allium ampeloprasum as an ethnomedicinal plant has many therapeutic effects in the traditional medicine. In the recent years, the silver nanoparticles green-mediated by ethno medicinal plants have been used for the treatment of several kinds of cancers. Now, we investigated the silver nanoparticles green-synthesized according to green chemistry rules using the aqueous extract of Allium ampeloprasum leaf for the treatment of lung carcinoma. The properties of silver nanoparticles against common human lung cancer cell lines i.e. HT144, SKMEL2, WM266-4, and IPC-298 were evaluated. The green-synthesized silver nanoparticles were characterized using different techniques such as FE-SEM, UV-Vis, and TEM. The FE-SEM and TEM results confirm spherical morphology for the nanoparticles with size of 11.9–46.7 nm. The IC50 of the silver nanoparticles was 125, 164, 180 and 149 µg/ml against HT144, SKMEL2, WM266-4, and IPC-298, respectively. After doing the clinical trial studies, the recent nanoparticles may be used as an anti-lung supplement in humans.

Deep Eutectic Solvents Comprising Organic Acids and Their Application in (Bio)Medicine.

Over the last years, we observed a significant increase in the number of published studies that focus on the synthesis and characterization of deep eutectic solvents (DESs). These materials are of particular interest mainly due to their physical and chemical stability, low vapor pressure, ease of synthesis, and the possibility of tailoring their properties through dilution or change of the ratio of parent substances (PS). DESs, considered as one of the greenest families of solvents, are used in many fields, such as organic synthesis, (bio)catalysis, electrochemistry, and (bio)medicine. DESs applications have already been reported in various review articles. However, these reports mainly described these components' basics and general properties without focusing on the particular, PS-wise, group of DESs. Many DESs investigated for potential (bio)medical applications comprise organic acids. However, due to the different aims of the reported studies, many of these substances have not yet been investigated thoroughly, which makes it challenging for the field to move forward. Herein, we propose distinguishing DESs comprising organic acids (OA-DESs) as a specific group derived from natural deep eutectic solvents (NADESs). This review aims to highlight and compare the applications of OA-DESs as antimicrobial agents and drug delivery enhancers-two essential fields in (bio)medical studies where DESs have already been implemented and proven their potential. From the survey of the literature data, it is evident that OA-DESs represent an excellent type of DESs for specific biomedical applications, owing to their negligible cytotoxicity, fulfilling the rules of green chemistry and being generally effective as drug delivery enhancers and antimicrobial agents. The main focus is on the most intriguing examples and (where possible) application-based comparison of particular groups of OA-DESs. This should highlight the importance of OA-DESs and give valuable clues on the direction the field can take.

The solid state synthesis of novel pyrazolyl-5-amino-pyrazole-4-carbonitrile derivatives using Fe3O4@SP@TA nanocomposites as a novel, green, and magnetically separable catalyst

Tannic acid‐functionalized silica‐coated Fe3O4 nanocomposites (Fe3O4@SP@TA) were synthesized and characterized by transmission electron microscope (TEM), field emission scanning electron microscope (FE-SEM), X-ray powder diffraction (XRD), X-ray spectroscopy (EDX), vibrating sample magnetometry (VSM) and Fourier transform infrared spectroscopy (FT-IR). Fe3O4@SP@TA nanocomposite supplies an environmentally friendly procedure for the synthesis of some novel pyrazolyl-5-amino-pyrazole-4-carbonitrile through a three-component mechanochemical reaction of synthetized pyrazolecarbaldehydes, malononitrile, and phenylhydrazine according to green chemistry rules. These compounds were obtained in high yields and short reaction times. The catalyst could be easily recovered and reused for six cycles with almost consistent activity. The structures of the synthesized pyrazolyl-5-amino-pyrazole-4-carbonitrile compounds were confirmed by 1H NMR, 13C NMR, and FTIR spectral data and elemental analyses.

White & grey biotechnologies for shaping a sustainable future

The developments in enzymology and biocatalytic processes designed as per the rules of green chemistry and green engineering are the keys to the concepts of biorefinery and bioeconomy which in turn enable sustainable development.

Green composites from vanillin-based benzoxazine and silane surface modified chopped carbon fibers

By following the rules of green chemistry, a novel composite is developed from a renewable and ecofriendly resource, namely, vanillin. The latter was used as a phenolic precursor for the microwave synthesis of a bio-based benzoxazine resin (Va-BZ). Afterward, high-performance green composites were developed by reinforcing Va-BZ with various amounts of chopped silane surface modified carbon fibers (CFs). The chemical structure of the Va-BZ monomers was confirmed by 1H NMR and Fourier transform infrared spectroscopy. The grafting of the silane moiety on the CF surface was assessed by FTIR and TGA analyses. The autocatalytic ring opening polymerization of the Va-BZ monomers was confirmed by DSC analysis. The mechanical performances of the developed green composites were studied by flexural and tensile investigations. The findings suggested that the maximum amount of 20 wt. CFs afforded the best results, with flexural and tensile strengths of 450 and 462 MPa, respectively. The SEM was used to study the fractured tensile surfaces and elucidated the toughening mechanism. Meanwhile, the TGA showed that the introduction of the CFs markedly improved the thermal stability of the benzoxazine matrix. Overall, this study confirmed that greener approaches can also result in high-performance composites satisfying the needs of exigent applications.

Mechanical and thermal properties of fully green composites from vanillin-based benzoxazine and silane surface modified chopped basalt fibers

By following the rules of green chemistry, a novel composite is developed from a renewable and ecofriendly resource, namely the vanillin. The latter was used as a phenolic precursor for the microwave synthesis of a bio-based benzoxazine resin (Va-BZ). Afterwards, high performance green composites were developed by reinforcing the Va-BZ with various amounts of chopped silane surface modified basalt fibers (BFs). The chemical structure of the Va-BZ monomers was confirmed by 1H NMR and FTIR spectroscopy. The grafting of the silane moiety on the BFs surface was assessed by FTIR and TGA analyses. The autocatalytic ring opening polymerization of the Va-BZ monomers was confirmed by DSC analysis. The mechanical performances of the developed green composites were studied by flexural and tensile investigations. The findings suggested that the maximum amount of 20 wt. BFs afforded the best results, with flexural and tensile strengths of 447 and 460 MPa, respectively. The SEM was used to study the fractured tensile surfaces and elucidated the toughening mechanism. Meanwhile, the TGA showed that the introduction of the BFs markedly improved the thermal stability of the benzoxazine matrix. Finally, the gamma rays shielding effectiveness was studied and revealed the highly benefic role of the BFs. For instance, a 1 cm thick Va-BZ polymer only showed a 6% gamma rays screening ratio, the latter was improved to 18.4% for the composite made of 20 wt.% of treated BFs. Overall, this study confirmed that greener approaches can also result in high performance composite satisfying the needs of exigent applications.

One-pot three component synthesis of pyrido[2,3-d]pyrimidines and benzo[4,5]imidazo[1,2-a]-pyrimidine-3-carbonitrile catalyzed by acidic ionic liquid immobilized on nanoporous TiO2

In the present study, a new as a heterogeneous, efficient, green and recyclable nano catalyst was synthesized via the immobilization of N,N-bis(3-(trimethylsilyl)propyl)octan-1-aminium hydrogen sulfate ionic liquid on nonporous TiO2 (TiO2-[bipocam]-HSO4) and was characterized by different analysis methods such as XRD, FESEM, TGA and FTIR. In continue, it was used in the three-component one pot synthesis of pyrido[2,3-d]pyrimidines and benzo[4,5]imidazo[1,2-a]-pyrimidine-3-carbonitriles derivatives. The main advantages of this method are compatibility with green chemistry rules, short reaction times (4-60 min.), high isolated yields (97-98%), easy product separation, use of non-toxic and safe catalyst, solvent-free conditions in reactions and reusability of the catalyst for five times without significant reduction in its catalytic activity.

Green synthesised CuNPs using Alhagi maurorum extract and its ability to amelioration of Mycoplasma pneumoniae infected pneumonia mice model

In this study, copper nanoparticles were synthesised according to green chemistry rules using the aqueous extract of Alhagi maurorum. The green-synthesised CuNPs were characterised using different techniques such as EDX, FE-SEM, XRD and FT-IR. The FE-SEM results confirm spherical morphology for the nanoparticles with a size of 42.68–68.87 nm. In vivo, the Mycoplasma pneumonia injection was applied for inducing pneumonia in the BALB/c mice, also the treatment was with CuNPs. The serum parameters such as inflammatory mediators (IL-6, TNF-α, IL-8, TGF and IL-1), total cell counts, and total protein content levels were evaluated. CuNPs regulated the levels of the inflammatory mediators in the infected mice. The cellular arrangements at the histopathological images were ameliorated with the administration of CuNPs. At the antioxidant test, the CuNPs and BHT removed 50% of free radicals at the concentrations of 181 and 110 µg/ml. In conclusion, the results revealed the ameliorative property of CuNPs green-formulated by A. maurorum aqueous extract on M. pneumoniae infected pneumonia mice model.

Anti-human ovarian cancer and cytotoxicity effects of nickel nanoparticles green-synthesized by Alhagi maurorum leaf aqueous extract

Alhagi maurorum (camelthorn) is one of the popular medicinal plants. The plant has many pharmaceutical uses in traditional medicine. In this study, nickel nanoparticles were synthesized according to green chemistry rules using the aqueous extract of A. maurorum. The green-synthesized NiNPs were characterized using different techniques such as EDX, FE-SEM, XRD, UV–vis and FT-IR. The FE-SEM results confirm spherical morphology for the nanoparticles with size of 20.56–36.63 nm. In the oncological part of the present study, the treated cells with NiNPs were assessed by MTT assay for 48 h about the cytotoxicity and anti-human ovarian cancer properties on normal (HUVEC) and ovarian cancer cell lines i.e. OVCAR-3, ES-2, TOV-21G, OV-90 and UWB1.289. The viability of malignant ovarian cell lines reduced dose-dependently in the presence of NiNPs. The IC50 of NiNPs was 191, 312, 250, 396 and 241 µg/mL against OVCAR-3, ES-2, TOV-21G, OV-90 and UWB1.289 cell lines respectively.