Treatment of nerve cancer with the green synthesis of CuO NPs

Abstract

With technological advances in molecular techniques and bioinformatics, many information has been gained that will help in the cancer early diagnosis. Studying and investigating agents of natural origin, such as plants nanocompounds is one of the most important research goals in the cancer treatment field. In the recent study, copper nanoparticles were synthesized according to green chemistry rules using the Boswellia thurifera aqueous extract. After doing the clinical trial studies, the recent nanoparticles may be used as an anti-carcinoma supplement in humans. The properties of copper nanoparticles against common human nerve carcinoma cell lines i.e. were determined. The green-synthesized copper nanoparticles were characterized using different techniques such as X-ray diffraction (XRD), energy dispersive X-ray (EDX), fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), and ultraviolet–visible spectroscopy (UV–Vis). The FE-SEM results confirm spherical morphology for the nanoparticles with size of 14.1 to 37.9 nm. The IC50 of the copper nanoparticles was 163 and 245 against S462 Human Peripheral Nerve Sheath Tumor and BL1391 cancer cells, respectively. Copper nanoparticles induce cell apoptosis, accompanied by the pro-apoptotic markers upregulation (cleaved caspase-8 and Bax) and anti-apoptotic marker downregulation, Bcl-2. Also, copper nanoparticles prevent colony formation compared to their matched control. More significantly, the molecular pathway analysis of copper nanoparticles-treated cells indicated that copper nanoparticles raises p53 expression, while preventing the expression of total and phosphorylated Signal Transducer and Activator Of Transcription 3 (STAT3) in cell lines, offering p53 and STAT3 are the main key players behind the biological events provoked by the extract in human cancer cells.