Sugarcane white grubs (Coleoptera: Scarabeidae) account for 75% of all insect pest losses to sugarcane in Australia. They damage roots causing lodging, reduced plant growth, and death of the cane plant. From the 10-12 major economic white grub species in Queensland, Antitrogus par&us is important in the Bundaberg-Isis districts. Both Mocap and SuSCon, the two insecticides used generally for white grub control, are less effective against A. parvulus than against other species, mainly because of the high (53-74%) clay content of the soil. Damage by A. parvulus occurs mostly in the second (final) year of the life cycle, with major impact from the third instar between September and February. Wild-type isolates of the fungus Metarhizium anisopliae were found to be highly pathogenic in the laboratory toward A. parvulus and other white grub species (K. D. Z. Samuels, D. E. Pinnock, N. E. Featherstone, and R. M. Bull, unpublished). A field trial was established to monitor the effect of field conditions on M. anisopliae spore applications and to assess crop protection potential. We report here significant field control of A. parvulus following M. anisopliae spore applications. Spores of M. anisopliue strain WF2X isolated from Lepidiota negatoria (Scarabaeidae: Melolonthinae) from Bundaberg, Australia, were mass produced on wheat, rice, or maize. Two-kilogram batches of sterile grain in autoclavable bags were each inoculated with 0.5 liter of a 5-day-old liquid culture of WF2X, and through which sterile air was passed for up to 1 month until heavily sporulated. The resultant culture on grain was applied to lOOto 125-mm-deep furrows along each side of the row of 6month-old first ratoon sugarcane var. CP44-101, then the soil was replaced. Doses of the unprocessed culture on grain were applied on 4 March 1986 at 1 x 10” spores/ha to 15-m-long plots of three cane rows each, set out in two 45-m columns (three treatments per column) separated by a three-row untreated control. Four replicates constituted culture on rice (A-D) with one each on wheat (E) and maize (F). Differences between means were tested by Student’s t test while goodness of tit was tested by x2 analysis, as indicated in the tables. Immediately prior to treatment, a series of 10 cane stools (45 x 45 x 30 cm deep) were dug by spade at random across the trial area and a population of 20 (2 10) larvae found beneath each. All three instars were found in approximately equal numbers. Soil samples were collected on eight occasions to October 1988 to monitor spore survival, while A. parvulus populations were periodically assessed from live 300mm cubes of soil dug from each plot (Table 1). No visible effect on crop vigor was detected in the first or second ratoon, despite A. parvulus larvae being present, with yields for both years of 90 tonnes cane/ha. The young third ratoon exhibited strong growth responses in the treated plots, while the untreated had retarded growth and yellow foliage (Table 2). Population assessment confirmed all treated plots (except C) had lower numbers of A. parvulus larvae by December 1987, with a number of infected cadavers being recorded prior to this during 1987 (Table 1). Treatment C inoculum was contaminated by a Penicillium sp. at application, which may have initially affected infectivity of the M. anisopliae spores. However, by March 1988, 3.88 x lo5 M. anisopliae spores/g soil were recorded in this plot. Two infected cadavers were found in
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