ABSTRACT Introduction Biobanking is an emerging concept in Egypt. Medical students represent important future stakeholders in the research community. The objective of this work was to evaluate the knowledge, attitude, and opinions of Egyptian medical students toward biobanking issues. Methods We designed a structured survey about these issues, which was communicated online with medical students at three universities in Egypt. Results A total of 315 of 364 questionnaires were completed. More than half the students have heard the term “biobanking” before. Approximately 77% had a general positive attitude toward the concept of biobanking; however, only 57.7% were willing to participate with their own or family samples. The positive attitude toward donation was significantly higher in participants with negative family history of inherited diseases (p = 0.02). Conclusion Although some students had limited knowledge of biobanking, they had a positive attitude toward the concept in general. However, this was not reflected in a similar attitude toward donating biospecimens, especially in those with a family history of inherited disease. Moreover, they expressed concerns about data protection and samples sharing with different stakeholders. Taking all these into account, educational activities about biobanking should be given to medical students to fill the knowledge gap on the subject.