Fusarium Wilt Of Tomato Research Articles

Biocontrol Activity of Aspergillus terreus ANU-301 against Two Distinct Plant Diseases, Tomato Fusarium Wilt and Potato Soft Rot.

To screen antagonistic fungi against plant pathogens, dual culture assay (DCA) and culture filtrate assay (CFA) were performed with unknown soil-born fungi. Among the different fungi isolated and screened from the soil, fungal isolate ANU-301 successfully inhibited growth of different plant pathogenic fungi, Colletotrichum acutatum, Alternaria alternata, and Fusarium oxysporum, in DCA and CFA. Morphological characteristics and rDNA internal transcribed spacer sequence analysis identified ANU-301 as Aspergillus terreus. Inoculation of tomato plants with Fusarium oxysporum f. sp. lycopersici (FOL) induced severe wilting symptom; however, co-inoculation with ANU-301 significantly enhanced resistance of tomato plants against FOL. In addition, culture filtrate (CF) of ANU-301 not only showed bacterial growth inhibition activity against Dickeya chrysanthemi (Dc), but also demonstrated protective effect in potato tuber against soft rot disease. Gas chromatography-tandem mass spectrometry analysis of CF of ANU-301 identified 2,4-bis(1-methyl-1-phenylethyl)-phenol (MPP) as the most abundant compound. MPP inhibited growth of Dc, but not of FOL, in a dose-dependent manner, and protected potato tuber from the soft rot disease induced by Dc. In conclusion, Aspergillus terreus ANU-301 could be used and further tested as a potential biological control agent.

Bacillus velezensis supernatant mitigates tomato Fusarium wilt and affects the functional microbial structure in the rhizosphere in a concentration-dependent manner

This study aimed to test the hypothesis whether the soil-application of Bacillus velezensis Bs006 and its supernatant affect the rhizospheric microbial community of tomato-Fusarium oxysporum f. sp. lycopersici pathosystem. The relationship between the functional response of the microorganisms measured through use of carbon sources and the disease development was determined under a dose-response test between Fusarium wilt development and Bs006-based treatments (spores at 1 × 107 and 1 × 109 cfu ml−1 and supernatant at 1 and 10%). Use of carbon sources by the microbial community in the rhizosphere at 3 and 30 days after treatment were determined through the Ecoplate Biolog® microplate assays. The efficacy against disease development was dependent on the concentrations of biocontrol treatments, with low concentrations (Supernatant-1% and Bs006-107 cfu ml−1) significantly reducing the incidence of tomato vascular wilt compared to the effect of the highest concentrations (Supernatant-10% and Bs006-109 cfu ml−1). The use of carbon sources expressed as the average metabolic response (AMR), by the rhizosphere microbial community was higher in the less effective treatments of biocontrol than the absolute control and the plants treated with low concentration of biocontrol treatments. The high AMR was related with low functional microbial diversity, expressed as the number of carbon sources consumed, which was also low in the rhizosphere of the negative control. On the contrary, the AMR was lower, and the functional microbial diversity was higher in the most effective treatments against Fusarium wilt. Functional microbial groups consuming carbohydrates, polymers and amines were associated with the less effective treatments, while the use of amino acids and carboxylic acids was associated with the most effective treatments. We conclude that treatments containing Supernatant-10% changed the rhizosphere microbial behavior for carbon sources consumption and it was stablished as a factor that promotes the tomato Fusarium-wilt development. This study contributes to the aspects to be considered in the generation of use directions of fungicides based on Bacillus spp.

Perception of Biocontrol Potential of Bacillus inaquosorum KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis.

Simple SummaryBacillus is a bacterial genus that is widely used as a promising alternative to chemical pesticides due to its protective activity toward economically important plant pathogens. Fusarium wilt of tomato is a serious fungal disease limiting tomato production worldwide. Recently, the newly isolated B. inaquosorum strain KR2-7 considerably suppressed Fusarium wilt of tomato plants. The present study was performed to perceive potential direct and indirect biocontrol mechanisms implemented by KR2-7 against this disease through genome and chemical analysis. The potential direct biocontrol mechanisms of KR2-7 were determined through the identification of genes involved in the synthesis of antibiotically active compounds suppressing tomato Fusarium wilt. Furthermore, the indirect mechanisms of this bacterium were perceived through recognizing genes that contributed to the resource acquisition or modulation of plant hormone levels. This is the first study that aimed at the modes of actions of B. inaquosorum against Fusarium wilt of tomatoes and the results strongly indicate that strain KR2-7 could be a good candidate for microbial biopesticide formulations to be used for biological control of plant diseases and plant growth promotion.Tomato Fusarium wilt, caused by Fusarium oxysporum f. sp. lycopersici (Fol), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against Fol was investigated through integrated genome mining and chemical analysis. Strain KR2-7 was identified as B. inaquosorum based on phylogenetic analysis. Through the genome mining of strain KR2-7, we identified nine antifungal and antibacterial compound biosynthetic gene clusters (BGCs) including fengycin, surfactin and Bacillomycin F, bacillaene, macrolactin, sporulation killing factor (skf), subtilosin A, bacilysin, and bacillibactin. The corresponding compounds were confirmed through MALDI-TOF-MS chemical analysis. The gene/gene clusters involved in plant colonization, plant growth promotion, and induced systemic resistance were also identified in the KR2-7 genome, and their related secondary metabolites were detected. In light of these results, the biocontrol potential of strain KR2-7 against tomato Fusarium wilt was identified. This study highlights the potential to use strain KR2-7 as a plant-growth promotion agent.

Effect of endophytic Bacillus and arbuscular mycorrhiza fungi (AMF) against Fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici

BackgroundFusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici (FOL) is a serious disease that causes significant economic losses in tomato production. Seventeen endophytic Bacillus isolates from tomato roots of Meghalaya were tested for antagonistic and plant growth promotion activities. Dominating arbuscular mycorrhiza fungi (AMF) spores were isolated from the rhizosphere soils of tomato grown in Meghalaya. The effect of different combinations of AMF and endophytic Bacillus on Fusarium wilt severity and growth of tomato plant under pot and field conditions was studied.ResultsThe endophytic Bacillus isolates ERBS51 and ERBS10 showed a maximum inhibition against FOL, with 58.43 and 55.68%, respectively, in a dual culture experiment. ERBS51 and ERBS10 were identified as Bacillus velezensis and Bacillus sp., respectively, based on 16s rRNA sequencing. Both isolates were found positive for iturin A, surfactin, bacillomycin D, protease, cellulase, pectinase, alpha-amylase, siderophore, ammonia production and ZnCO3 solubilization. Funneliformis mosseae and Glomus fasciculatum were the dominating AMF species in tomato rhizosphere of Meghalaya. The result of pot and field experiments revealed that out of all the treatments, combination of Funneliformis mosseae + Glomus fasciculatum + Bacillus velezensis + Bacillus sp. was shown to be the best in reducing the severity of Fusarium wilt to 77.44 and 66.74%, respectively. F. mosseae + G. fasciculatum + B. velezensis + Bacillus sp. also recorded the highest in most growth attributes and yield.ConclusionsEndophytic Bacillus (B. velezensis and Bacillus sp.) and AMF (F. mosseae and G. fasciculatum) were safe and effective biocontrol agents against Fusarium wilt of tomato.

Novel Sources of Resistance to Fusarium oxysporum f. sp. lycopersici Race 3 Among Solanum pennellii Accessions

Fusarium wilt of tomato (Solanum lycopersicum), caused by fungal pathogen Fusarium oxysporum f. sp. lycopersici (Fol), is one of the most important diseases in tomato production. Three races of the pathogen are described, and race-specific resistance genes have been applied in commercial tomato cultivars for controlling the disease. Race 3 (Fol3) threatens tomato production in many regions around the world, and novel resistance resources could expand the diversity and durability of Fol resistance. The wild tomato species, Solanum pennellii, is reported to harbor broad resistance to Fol and was the source of two known Fol3 resistance genes. In this study, we evaluated 42 S. pennellii accessions for resistance to each fusarium wilt race. F1 plants, developed from crossing each accession with the Fol3 susceptible line ‘Suncoast’, were evaluated for Fol3 resistance, and BC1F1 plants were screened to determine the likelihood that Fol3 resistance was based on a novel locus (loci). Nearly all accessions showed resistance to Fol3, and many accessions were resistant to all races. Evaluation of F1 plants indicated a dominant resistance effect to Fol3 from most accessions. Genetic analysis indicated 24 accessions are expected to contain one or more novel Fol3 resistance loci other than an allele near the I-3 locus. To investigate genetic structure of the S. pennellii accessions used in this study, we genotyped all 42 accessions using genotyping by sequencing. Approximately 20% of the single nucleotide polymorphism (SNP) loci were heterozygous across accessions, likely due to the outcrossing nature of the species. Genetic structure analysis at 49,120 unique SNP loci across accessions identified small but obvious genetic differentiations.

The efficacy of various fungicides against tomato Fusarium wilt (Fusarium oxysporum f. sp. lycopersici)

The tomato is one of the most popular vegetables farmed worldwide. One of the most harmful tomato disease is Fusarium wilt, which is a disease that the fungus Fusarium oxysporum f.sp. lycopersici causes. Disease causes about 30% to 40% losses in yield. The poisoned food technique was used to assess the fungitoxic activity of the fungicides Blitox 50, Aliette, Roko, Amistar, Avtar, and Cabrio Top at the plant pathology department of the college of agriculture at LPU, Punjab, throughout the years 2021-2022. Six different fungicides were tested in the lab at various concentrations such as 50, 100, and 150 ppm. Thiophanate Methyl (Roko) show the best result in laboratory. Same fungicides were checked at field. Among this fungicides Copper Oxychloride (Blitox 50) shows the best result in field condition.

Management of tomato wilt caused by Fusarium oxysporum f. sp. lycopersici through organic amendments

The present study explores the potential of different organic amendments i.e. vermicompost, spent compost mushroom, calotropis leaf extract, panchgavya and Trichoderma against tomato fusarium wilt caused by Fusarium oxysporum f. sp. lycopersici in pots under greenhouse conditions. The population of F oxysporum f. sp. lycopersici was significantly reduced after application of organic amendments. Drenching of soil with Trichoderma asperellum @10 g-1 + Chitosan @ 0.1% (T3) recorded lowest percent disease index (34.40 %) and was at par with chemical control with carbendazim @ 0.1%. Treatment (T1) soil application of FYM + Trichoderma asperellum @ 2% w/w and (T2) vermicompost @ 2 % w/w were also effective in minimizing the disease and PDI recorded at 43.30 and 46.63, respectively. Panchgavya @ 30 per cent was also at par with vermicompost recording 49.96 per cent PDI. Maximum PDI was recorded in control (73.30%).

Evaluation of Fungicides against Fusarium oxysporum f.sp. lycopersici the Cause of Fusarium Wilt of Tomato

Tomato is very significant and full of nutrition vegetable crop. It is enriched with vitamin a and C, lycopene, and protects from prostate cancer, cardiovascular diseases. Tomato wilt disease (TWD) caused by F. oxysporum f.sp. lycopersici (Fol) is a serious threat to tomato crop in Pakistan and spreading to those areas in which it was not previously present. The current research was planned to evaluate the fungi-toxic activity of fungicides against F. oxysporum in vitro and under greenhouse conditions. Four fungicides which include Carbendazim, Benomyl, Curzate and Nativo, at 500ppm, 750ppm and 1000ppm concentrations, were evaluated using food poison technique. The overall results showed that all fungicides significantly (P < 0.05) inhibited mycelial growth of F. oxysporum, however, Nativo proved to be best followed by Carbendazim, Benomyl at all concentrations. The reduction in colony growth of pathogen gradually increased by increasing the fungicide concentration. Two fungicides, Nativo and Carbendazim and their concentrations 750 and 1000 ppm, which proved to be effective in laboratory conditions, also evaluated under greenhouse conditions. Both fungicides effectively controlled TWD under greenhouse conditions. The present research revealed that Nativo and Carbendazim at 750 and 1000 ppm concentrations are effective against Fol, thus may be included in management strategy of TWD.

Prevalence of Fusarium Wilt of Tomato in Major Tomato Growing Areas of Punjab, Pakistan

Tomato is an essential vegetable in the world due to its nutritional importance. It has vitamin A C and lycopene, which reduce the prostate cancer, and heart and age-related diseases. Tomato wilt disease (TWD) is a potential threat and spreading rapidly in tomato fields in Pakistan. The survey on the disease prevalence of Fusarium wilt (Fusarium oxysporum f.sp. lycopersici (Fol)) of tomato was conducted in the major tomato growing areas of Punjab, viz., Hafizabad, Pakpattan, Khushab, Sargodha and Faisalabad districts during the cropping season 2018. Tomato wilt disease incidence was higher in district Sargodha (94%) followed by Faisalabad (92%) and Khushab (65%). Two districts, Pakpattan and Hafizabad showed 86% and 73% incidence, respectively. The survey revealed that tomato disease incidence was prevalent with varying intensities in all surveyed districts of Punjab. Moreover, the variation in disease prevalence of tomato wilt disease in different locations was due to the environmental conditions of that area and the varieties being cultivated.

Fusarium wilt and Southern Blight Diseases of Tomatoes: Trichoderma spp. as Antagonists of the Causative Organisms

Fusarium wilt ( Fusarium oxysporum f. sp . lycopersici ) and southern blight ( Sclerotium rolfsii ) are two of the soil borne diseases limiting tomato cultivation globally and are difficult to manage. This study evaluated biocontrol agents, Trichoderma harzianum and T. viride for antagonistic efficacy against these pathogens in vitro . The dual culture technique was adopted using two approaches; namely, pre-inoculation of Trichoderma spp. into culture medium at 24 and 48 hours ahead of the pathogen and simultaneous inoculation of Trichoderma spp. and pathogen. The Trichoderma spp. were evaluated singly against each pathogen. The sole culture of each pathogen was the control. Each treatment was replicated thrice and laid out in a completely randomized design. Inoculated Petri plates were incubated at 27 o C ± 2 o C. Data collected were subjected to statistical analysis and mean separation using Minitab software. Results showed that the inhibition of the mycelial growth of both pathogens by the biocontrol agents were days dependent. Furthermore, prophylactic evaluations gave significantly higher inhibition of the mycelial growth of the pathogens as against therapeutic evaluation. Additionally, F. oxysporum was more susceptible to antagonism by both biocontrol agents as higher percentages of mycelial growth inhibition were recorded for it in all treatments. Finally, T. harzianum was a better antagonist of the two pathogens, recording 84.62% and 69.01% inhibition of mycelial growth of F. oxysporum and S. rolfsii, respectively at 9 days after inoculation. Trichoderma spp . maybe exploited as biocontrol agents for the management of these pathogens.

Breeding for Resistance to Fusarium Wilt of Tomato: A Review.

For over a century, breeders have worked to develop tomato (Solanum lycopersicum) cultivars with resistance to Fusarium wilt (Fol) caused by the soilborne fungus Fusarium oxysporum f. sp. lycopersici. Host resistance is the most effective strategy for the management of this disease. For each of the three Fol races, resistance has been introgressed from wild tomato species, predominately in the form of R genes. The I, I-2, I-3, and I-7 R genes have each been identified, as well as the corresponding Avr effectors in the fungus with the exception of Avr7. The mechanisms by which the R gene protein products recognize these effectors, however, has not been elucidated. Extensive genetic mapping, gene cloning, and genome sequencing efforts support the development of tightly-linked molecular markers, which greatly expedite tomato breeding and the development of elite, Fol resistant cultivars. These resources also provide important tools for pyramiding resistance genes and should support the durability of host resistance.

The bioprotective effect of root endophytic Serendipita herbamans against Fusarium wilt in tomato and its impact on root traits are determined by temperature

Environmental conditions such as temperature can be important determinants for the success of biocontrol agents in field situations. On the one hand temperature affects biocontrol strains on the other plant–pathogen dynamics. In order to understand these dynamics, driving factors behind these interactions need to be fully characterized. Here, we i) investigated the effect of two temperature treatments on the biocontrol activity of the root endophyte Serendipita herbamans against the soil-borne pathogen Fusarium oxysporum f. sp. lycopersici (Fol) in tomato and ii) assessed the impact of this biocontrol system on root morphological traits. The results of this study indicate that inoculation with S. herbamans stimulated shoot length and enhanced the shoot and root dry mass in tomato plants across both temperatures. Furthermore, S. herbamans inoculation decreased disease incidence from 67% in the Fol only treatment to 22% at lower temperatures (24 °C). At 28 °C, disease incidence was around 41% and no reduction could be seen; however, root morphological traits such as root length, root surface area and root volume were not affected at 24 °C but severely reduced under 28 °C upon Fol inoculation. A reduction of root morphological parameters was not observed in S. herbamans + Fol treated plants at 28 °C indicating a protective effect against Fol. Moreover, root morphological traits appear to be more influenced by the different temperatures than root dry weights. In additional in-vitro experiments, growth promoting effects of S. herbamans on root traits without direct contact were evident indicating the involvement of volatile organic compounds in growth promotion. In conclusion, S. herbamans provides protection against the temperature-dependent damage Fol inflicts on the tomato plants by reducing disease development under 24 °C and by mitigating reduction in root morphological parameters under 28 °C in the early weeks of interaction.

RNAi-mediated silencing of PEX6 and GAS1 genes of Fusarium oxysporum f. sp. lycopersici confers resistance against Fusarium wilt in tomato.

The online version contains supplementary material available at 10.1007/s13205-021-02973-8.

Effect of carbon-based nanomaterials on Fusarium wilt in tomato

Fungal diseases often cause significant losses in tomato cultivation. Fusarium oxysporum is responsible for substantial losses in the yields of tomato crops growing in open fields and in greenhouses. Carbon-based nanomaterials (CNMs) have been widely used in numerous applications in agriculture and are proposed as a potential alternative for disease control. In the present study, two carbon-based nanomaterials were evaluated on the incidence and severity of F. oxysporum and on components of the antioxidant defense system in tomato plants. The results showed that the application of CNMs reduced the severity of F. oxysporum, and increased the yield of tomato fruits, the content of photosynthetic pigments, ascorbic acid and flavonoids, and the activity of the antioxidant enzymes in the leaves of plants inoculated with this pathogen. These results suggest the application of CNMs may be a viable option for the control of diseases such as F. oxysporum in tomato crops.

Biotic elicitor induced nitric oxide production in mitigation of Fusarium wilt of tomato

Biotic elicitor induced nitric oxide production in mitigation of Fusarium wilt of tomato

Effects of mixed culture fermentation of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the biocontrol of tomato Fusarium wilt.

To study the effects of mixed culture fermentation (MCF) of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the application values for agricultural production, with the intention of developing efficient and environmentally friendly biocontrol agents. In this study, an in vitro antifungal growth experiment showed that the inhibitory rate of the MCF broth on pathogenic fungi (Fusarium oxysporum f. sp. lycopersici, Botrytis cinerea, Trichothecium roseum and Colletotrichum gloeosporioides) was less than that of B. amyloliquefaciens culture fermentation (BCF). Moreover, the content and gene expression of lipopeptide antibiotics were also lower than that in the BCF group. However, the pot experiments based on irrigation with appropriately diluted fermentation broth showed that the biocontrol effect of MCF on tomato Fusarium wilt was significantly higher than that of TCF (T. longibrachiatum culture fermentation) and BCF, and was approximately 15.79% higher than that of the BTF group which made by mixing equivalent amounts of BCF and TCF. In MCF broth, two micro-organisms antagonized and coexisted, and the growth of T. longibrachiatum was inhibited. Using transcriptomic analysis, we speculated that MCF can upregulate the expression of genes related to carbon and nitrogen metabolism, oxidation-reduction activity, sporulation, environmental information response and chemotaxis, and biosynthesis of secondary metabolites of B. amyloliquefaciens, which might enhance the nutrient substances metabolism and competitiveness, survival ability, colonization and adaptability to the environment to increase its biocontrol potential. Mixed culture fermentation could promote the more reasonable and effective utilization of biocontrol micro-organisms though improving biocontrol effect, enhancing strains survival and competitiveness, increasing beneficial metabolites, combined with resistance induction or synergistic control. Using MCF agronomically utilizes biocontrol agents in an efficient way, which has a good potential for commercial implementation and could reduce production costs.

Suppressive effect of secondary metabolites from Streptomyces plumbeus isolate F31D against Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt of tomato

Suppressive effect of secondary metabolites from Streptomyces plumbeus isolate F31D against Fusarium oxysporum f. sp. lycopersici, the causal agent of Fusarium wilt of tomato

RETRACTED ARTICLE: Effects of vermicompost on tomato Fusarium wilt and soil microbial community structure

This article has been retracted from publication in the Taylor & Francis journal, Acta Agriculturae Scandinavica, Section B — Soil & Plant Science. Following publication, concerns were raised by multiple third-parties around the content of the special issue and the decision-making process. Following an investigation by the Taylor & Francis Publishing Ethics & Integrity team in full cooperation with the Editor-in-Chief, it was confirmed that this article included in Special Issue titled “ Envisage Computer Modelling and Statistics for Agriculture”, guest edited by Gunasekaran Manogaran was not peer-reviewed appropriately, in line with the Journal's peer review standards and policy. As the stringency of the peer review process is core to the integrity of the publication process, the Editor and Publisher have decided to retract all of the articles within the above-named Special Issue. The journal has not confirmed if the authors were aware of this compromised peer review process. The journal is committed to correcting the scientific record and will fully cooperate with any institutional investigations into this matter. The authors have been informed of this decision. We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines.

Biological Effects of Trichoderma spp. Isolated from Tomato Rhizosphere and Trichomax-HV Biofungicide against Tomato Fusarium Wilt in Vitro

پژمردگی فوزاریومی گوجه‏ فرنگی از مهم‌ترین و شایع‏ترین بیماری‏های گوجه ‏فرنگی در مناطق زیر کشت این گیاه در سراسر جهان می‏باشد. کنترل بیولوژیک بیماری های گیاهی با استفاده از میکروارگانیسم های غیر بیماری‌ زا توجه بسیاری از پژوهشگران را به خود جلب نموده است. از موفق‏ترین و پرکاربردترین میکروارگانیسم ‏هایی که باعث جلوگیری از خسارت قارچ‏ها در گیاهان می‏شوند، گونه‏های مختلف جنس Trichoderma می‏باشند. در این تحقیق اثر دو جدایه Trichoderma harzianum و Trichoderma virens و سم بیولوژیک Trichomax-HV علیه Fusarium oxysporum f. sp. lycopersici عامل بیماری پژمردگی فوزاریومی گوجه فرنگی در آزمایشگاه و گلخانه مورد ارزیابی قرار گرفت. فعالیت آنتاگونیستی این جدایه ها علیه این بیمارگر در آزمایشگاه به روش کشت متقابل مورد مطالعه قرار گرفت. به ‌منظور بررسی اثر این جدایه های آنتاگونیست در گلخانه، ابتدا مایه تلقیح قارچ بیمارگر به یک‌سوم تحتانی خاک گلدان‏ها افزوده شد و به‌ محض انتقال دادن گیاهچه‏ ها به گلدان، آنتاگونیست‏ های قارچی به گلدان‌ها اضافه شد و پس از 60 روز برهم‏کنش بین بیمارگر و آنتاگونیست، مورد ارزیابی قرار گرفت. نتایج بررسی‏ های آزمایشگاهی نشان داد که T. harzianum به میزان 9/48 درصد، Trichomax- HV به میزان 6/45 درصد و T. virens به میزان 36/22 درصد از رشد بیمارگر (در مقایسه با شاهد) جلوگیری کردند و باعث کلونیزه کردن پرگنه‏ های بیمارگر شدند. عوامل آنتاگونیست در آزمایش‏های گلخانه‏ ای موجب افزایش ارتفاع ساقه، وزن تر و خشک اندام های هوایی و ریشه در غیاب بیمارگر شدند. فقط T. harzianum در برهم‏کنش‏ با بیمارگر باعث افزایش ارتفاع ساقه، وزن تر و خشک اندام های هوایی و ریشه نسبت به شاهد آلوده شد.

Mycoparasitic nature of Egyptian Trichoderma isolates and their impact on suppression Fusarium wilt of tomato

BackgroundPlant disease administration is difficult due to the soil-borne nature of the phytopathogens. Biological control of plant disease is a safe mode to avoid the problems related to fungal diseases that affect crops productivity.ResultsTwenty-three Trichoderma isolates were isolated from soil, surrounding healthy tomato roots from different regions in the Egyptian Governorate of Menoufia. Using a dual culture method to test the efficiency of Trichoderma isolates, the most effective isolate identified as Trichoderma atrovirde with percentage inhibition against Fusarium oxysporum f. sp. lycopersici (92.11%) and scanning electron microscope examination documented the mycoparasitic nature of T. atrovirde to F. oxysporum. Treatments with 10% filtrate T. atrovirde improved the growth aspects of tomato plants than the control plants or infected only, as well as the increase in phenol content (15.09 ug. g-1dry weight) and decreased disease incidence percentage (8%) than the plants infected only (60%).ConclusionsThis study clearly demonstrated that T. atrovirde had a significant inhibition against F. oxysporum. Greenhouse assays displayed the protective role of T. atrovirde inoculation directly against pathogen or indirectly related to the defense mechanism in the plant. So, this study recommends using T. atrovirde for biological control of wilt disease in tomato plants.