Free Range Research Articles

Possible Outbreak of Newcastle Virus Infection in the Face of Increasing Trend of Backyard Poultry Farm Management Interface in Nigeria: A Public Health and Food Security Threat

Introduction: Newcastle disease is a highly contagious illness and one of the "notifiable illnesses" on the World Health Organization list for Animal Health (OIE) Outcome. There are constantly new cases being reported as new NDV isolates from around the world. Newcastle disease poses a significant global economic threat to the poultry sector, causing highly contagious and transmissible diseases in birds with huge food security threat among the weak and vulnerable developing and underdeveloped countries Aim: The aim of this article borders on bringing to light the risk of possible Newcastle disease outbreak in Nigeria due to increasing poor management of poultry farms and poultry waste product. Furthermore, it will also underscore and outline various ways which can be adopted to prevent this poultry disease which is of great public health, economic and food security protection importance. Method/Methodology: This is a systematic review of published research articles focusing on Newcastle disease, epidemiology, pathogenicity, transmission and factors that can play important roles in the possible outbreak of the disease in poultries. Studies reviewed comprise of cross-sectional, prospective, longitudinal and observational studies. The study approach follows the systematic review of peer-reviewed published articles as well as online publications and articles derived from various databases with search keywords relevant to the topic of discourse. Findings and Results: The findings in this study highlight the endemicity of Newcastle virus disease in various part of the country, and how the severity of the disease is influenced by host characteristics, viral properties, and environmental factors. The study pointed out critical evidence-based possible reasons that could promote a new round of Newcastle disease epidemic outbreak as a result of poor poultry management practices such as high bird densities, poor poultry infrastructure, lack of quarantine of sick birds, mixed species rearing, free range system of poultry, poor waste disposal, lack of expertise, and insufficient biosecurity outcomes. However, the lack of education and awareness among farmers exacerbates the problem, hindering preventive measures. The complexity is increased by difficulties with vaccination, unrestrained bird movement through trading with neighboring countries and interstate, and a lack of diagnostic facilities. The consequences of an outbreak extends beyond financial losses to potential disruptions in the poultry supply chain, food security risks, and public health concerns. Conclusion and Recommendation: The growing difficulties in poultry management methods increases the serious public health concern posed by a possible Newcastle Virus epidemic outbreak in Nigeria. A paradigm shift in poultry farming practices is necessary to address this problem. A comprehensive strategy that incorporates infrastructural development, regulatory frameworks, and education in order to strengthen the poultry industry is needed, so as to address and prevent the looming public health and food security crises that may break out from the above scenario

Modification of Humeral Component Results in Increased Impingement Free Range of Motion in a Reverse Shoulder Arthroplasty Model.

Shoulder arthroplasties have been demonstrated to provide reliable pain relief as well as functional benefits. The advent of the reverse shoulder arthroplasty allowed for expanded indications for shoulder replacement. Several studies comparing the outcomes of anatomic and reverse total shoulder arthroplasties have demonstrated decreased range of motion in the reverse arthroplasty cohort, especially in internal rotation. The authors hypothesized that slight modifications to the humeral component of a reverse shoulder arthroplasty could result in increased impingement free range of motion without significant sacrifices to stability. A reverse shoulder arthroplasty model was fashioned to mimic a setting of anterior mechanical impingement after replacement. Sequential resections were taken from the anterior aspect of the polyethylene up to a resection of 10 mm. A solid modeling software was utilized to compare the experimental group to the control group with regard to impingement free motion. Finite element analysis was subsequently utilized to assess stability of the construct in comparison to the nonmodified polyethylene. Impingement free internal rotation increased minimally at 3 mm of resection but considerably at each further increase in resection. A resection of 10 mm resulted roughly 30% improvement in impingement free internal rotation. Instability in this model increased with modifications beyond 7 mm. Slight alterations to the geometry of the humeral tray and polyethene components can result in improvements in impingement-free internal rotation without substantial increased instability in this model. Further work is needed to determine in vivo implications of modifications to the humeral tray and polyethylene.

Crop Residue as Optimum Feed Sourcing for Livestock Kept Under Free Range Conditions Within the Blue Nile Basin of Sudan

Crop Residue as Optimum Feed Sourcing for Livestock Kept Under Free Range Conditions Within the Blue Nile Basin of Sudan

Identification and bioinformatic analysis of invA gene of Salmonella in free range chicken.

Salmonella is a serious cause of the health issues in human and animal worldwide. Salmonella has been isolated from different biological samples and it considers as the key role in induction of inflammation of gastrointestinal tract which in turn cause diarrhoea in different species. To further understand the involvement of Salmonella in contaminating and infecting fresh eggs and meat of free-range chicken. This study aimed to establish the microbiological and molecular detections of Salmonella in the cloaca of the free-range chicken and to identify predicted biological functions using Kyoto Encyclopedia of Gene and Genomic (KEGG) pathways and protein-protein interaction. Cloacal swabs were collected from free range chicken raised in the local farm in Duhok city. The isolates were cultured and biochemical test performed using XLD and TSI, respectively. Molecular detection and functional annotation of invA gene was carried out using Conventional PCR and bioinformatics approaches. The present study found that Salmonella was detected in 36 out of 86 samples using microbiological methods. To confirm these findings, invA gene was utilised and 9 out of 36 Salmonella isolates have shown a positive signal of invA by agarose gel. In addition, bioinformatic analysis revealed that invA gene was mainly associated with bacterial secretion processes as well as their KEGG terms and Protein-Protein Interaction were involved in bacterial invasion and secretion pathways. These findings suggested that invA gene plays important role in regulating colonization and invasion processes of Salmonella within the gut host in the free range chicken.

Enhancing CO2 Hydrogenation Using a Heterogeneous Bimetal NiAl-Deposited Metal-Organic Framework NU-1000: Insights from First-Principles Calculations.

The hydrogenation of CO2 to high-value-added liquid fuels is crucial for greenhouse gas emission reduction and optimal utilization of carbon resources. Developing supported heterogeneous catalysts is a key strategy in this context, as they offer well-defined active sites for in-depth mechanistic studies and improved catalyst design. Here, we conducted extensive first-principles calculations to systematically explore the reaction mechanisms for CO2 hydrogenation on a heterogeneous bimetal NiAl-deposited metal-organic framework (MOF) NU-1000 and its catalytic performance as atomically dispersed catalysts for CO2 hydrogenation to formic acid (HCOOH), formaldehyde (H2CO), and methanol (CH3OH). The present results reveal that the presence of the NiAl-oxo cluster deposited on NU-1000 efficiently activates H2, and the facile heterolysis of H2 on Ni and adjacent O sites serves as a precursor to the hydrogenation of CO2 into various C1 products HCOOH, H2CO, and CH3OH. Generally, H2 activation is the rate-determining step in the entire CO2 hydrogenation process, the corresponding relatively low free energy barriers range from 14.5 to 15.9 kcal/mol, and the desorption of products on NiAl-deposited NU-1000 is relatively facile. Although the Al atom does not directly participate in the reaction, its presence provides exposed oxygen sites that facilitate the heterolytic cleavage of H2 and the hydrogenation of C1 intermediates, which plays an important role in enhancing the catalytic activity of the Ni site. The present study demonstrates that the catalytic performance of NU-1000 can be finely tuned by depositing heterometal-oxo clusters, and the porous MOF should be an attractive platform for the construction of atomically dispersed catalysts.

IDADA: towards a multimethod methodological framework for PhD by publication underpinned by critical realism

PhD by publication, a route of attaining a ‘doctorate’, is growing in adoption, yet has not gained universal acceptance. Whilst doctoral students are expected to free range, be independent in their study, and contribute to knowledge, there is a tendency to be schooled or mainly assessed along the lines of positivism and interpretivism. This paper, in part the author’s situational reflexivity and noting Liezel Frick’s “PhD by publication – panacea or paralysis?”, carried out a documentary analysis of academic regulations of 101 universities in the UK, South Africa, Ireland, Australia, and examined the literature for demi-regularities supporting or inhibiting critical realism in doctoral studies. It followed a (multimethod) plural approach. The examination revealed that critical realism is sparse in doctoral studies, and universities do not cohere in nomenclature and ‘form’ of PhD by publication. In filling the ‘free range’ gap, this paper contributes to the ongoing discourse on PhD by publication by introducing the IDADA (‘identify’, ‘define’, ‘analyse’, ‘develop’ and ‘apply’) methodological framework. The framework evolved from the demi-regularities understudied. IDADA is a practical solution-centric, methodological approach to PhD by publication. The paper calls on researchers and practitioners to be open to structured and ‘alternate’ approaches to research whilst ensuring ontological, epistemological, and axiomatic rigour. Researchers need not approach research religiously — as that of a particular paradigm sect; but rather embrace ‘practical adequacy’. Doctoral students should be encouraged to produce research output with more significant impact through interrogation and unearthing phenomena rather than just “observing, outlining and discussing findings” or conforming to ‘writing style’.

Interfacial thermal conductance of multilayer graphene/MoS2 heterostructure

We investigated the Interfacial thermal conductance (ITC) with a heterogeneous structure of a bilayer of MoS2 embedded in the middle of four graphene layers by non-equilibrium molecular dynamics (NEMD). Surprisingly, the ITC of MoS2/MoS2 (M/M) is significantly higher than that of the graphene/MoS2 (G/M). Interestingly, changes in the cross-sectional dimensions do not affect the ITC of the G/M and M/M, as the constant interface spacing preserves their structural characteristics so that phonon mean free range remains unchanged. Intriguingly, the interfacial strain affects the ITC notably. A 10% compression strain increases the G/M ITC by 100%, while a 10% tension strain reduces it by 70%. Unexpectedly, increasing defect concentration near the G/M interface leads to a decrease in thermal conductance. We find that graphene plays a dominant role in heat conduction within the heterostructure, while defects primarily enhanced low-frequency phonon vibrations of graphene, with MoS2 vibrations remaining largely unaffected. Due to the increase in defect rate, the out-of-plane phonon coupling is weakened, thus leading to a decrease in G/M ITC. These findings provide concise insights into the heat conduction of G/M heterostructures.

Reasonable mechanical model on shallow tunnel excavation to eliminate displacement singularity caused by unbalanced resultant

When considering initial stress field in geomaterial, nonzero resultant of shallow tunnel excavation exists, which produces logarithmic items in complex potentials, and would further lead to a unique displacement singularity at infinity to violate geo-engineering fact in real world. The mechanical and mathematical reasons of such a unique displacement singularity in the existing mechanical models are elaborated, and a new mechanical model is subsequently proposed to eliminate this singularity by constraining far-field ground surface displacement, and the original unbalanced resultant problem is converted into an equilibrium one with mixed boundary conditions. To solve stress and displacement in the new model, the analytic continuation is applied to transform the mixed boundary conditions into a homogenerous Riemann-Hilbert problem with extra constraints, which is then solved using an approximate and iterative method with good numerical stability. The Lanczos filtering is applied to the stress and displacement solution to reduce the Gibbs phenomena caused by abrupt change of the boundary conditions along ground surface. Several numerical cases are conducted to verify the proposed mechanical model and the results strongly validate that the proposed mechanical model successfully eliminates the displacement singularity caused by unbalanced resultant with good convergence and accuracy to obtain stress and displacement for shallow tunnel excavation. A parametric investigation is subsequently conducted to study the influence of tunnel depth, lateral coefficient, and free surface range on stress and displacement distribution in geomaterial.

Shape and fractures of carina sterni in chicken genotypes with different egg deposition rates reared indoor or free-range

Commercial laying hens have high frequency of damage to the keel bone (KB), which causes negative effects on health and welfare. KB damage may consist in fractures (KBF) and deviations (KBD). The aim of the present study was to compare the KB shape, by means of Geometric Morphometric, and the occurrence of fractures in different chicken genotypes reared either free-range (FR) or in enriched cages. Moreover, the relationship between KB shape, genotype and rearing system was analysed. Sixty birds/genotype (2 Italian local breeds, Bionda Piemontese and Robusta Maculata, their crossbreeds with Sasso and Lohmann Brown) were used. All the hens fed the same commercial feed throughout the trial. Body weight, egg production, feed intake and mortality were recorded from 25 to 66 weeks of age. Ca intake (IN) and output (OUT) were estimated and Ca OUT/IN was calculated. FR affected the occurrence of KB deviations but not the shape, whereas the fractures were mainly affected by genotype. Local breeds had a lower prevalence of KBF with similar level of KBD but with different shapes. Crossbreeds seemed to be a suitable compromise between egg deposition rate and occurrence of KB damages.

A 102.1-dB SNDR oversampling merge-mismatch-error-shaping SAR ADC in 180 nm CMOS

In this paper, we propose a merge-mismatch-error-shaping (M-MES) noise shaping SAR ADC as a solution to address the limitation of SNDR caused by the non-linearity resulting from DAC mismatch. The proposed M-MES technique is based on MES with the introduction of capacitive merging to mitigate the degradation of dynamic range (DR) in ADCs. In addition, to meet the high signal-to-noise ratio (SNR) and low power requirements of the ADC, cascaded integrator feed-forward (CIFF) noise shaping ADC in this paper is a first-order filter based on a closed-loop amplifier. The proposed ADC was simulated and designed in a 180-nm CMOS process, which consumes 449 μW of power when operating at 1 MS/s sampling frequency. According to the post-simulation results, under a 3.3-V supply and at an oversampling ratio (OSR) of 500, the proposed ADC achieves Schreier FoMS of 168.5 dB with 102.1 dB signal to noise and distortion ratio (SNDR) and 102.2 dB spurious free dynamic range (SFDR).

Experimental Analysis on Performance of Speech Utterance recognition using AI Models

In Automated speech recognition of the system performance is crucial and important to satisfy multiple requirements of HMI and, more recently, even in IoT-related applications as well. Concurrently, there has been an increase in demand for detecting strong critical features derived from speech utterances. This paper presents a performance of the developed machine learning algorithms with respect to audio digit speech recognition and classification. The prepared dataset contains a free range of words (from 1 to 10) from speakers of different age groups. The Audacity software used for preprocessing the audio files that includes removal of noise included in the signal and trimming the silence on either side of the word utterance. audio signal sampled at fs = 48kHz.We have developed four AI Models to recognise the word utterances. Audio signals are processed separately and derived two unique feature sets that includes statistical features set and singular values by performing SVD related to word utterances. The cepstral values for each utterance are obtained from state-of-the-art MFCC. Variance-covariance matrix is calculated from the generated MFCC matrix. The diagonal values which form the variance are recorded and denoted as feature set-1 for the word utterance and inputted to the machine learning algorithms. Performance matrices of the developed models are recorded. To keep the computational bottleneck associated with the use of feature sets to minimum, dimensionality reduction is carried out by applying singular value decomposition to the extracted MFCC matrix. The derived set of singular values ​​considered as feature set-2 is used to train and test the developed AI models with a ratio of 70:30. We presented and discussed the performance and results produced by MLP, KNN, SVM, Random Forest algorithms. In comparison, MLP and Random Forest were found to show excellent performance on both feature sets with 100% training accuracy and 99% test accuracy.

Isolation, identification, and molecular characterization of probiotic bacteria from locally selected Ethiopian free range chickens gastrointestinal tract

The poultry industry is facing continuous challenges with regard to increased feed costs and loss due to infectious disease. To overcome this challenge, several antibiotics have been used along with chicken feeds to promote growth. Nevertheless, the use of antibiotics as growth promoter has been banned in many countries, due to the concerns associated with potential risks of emerging and horizontal transfer of multidrug resistant genes to bacteria in animal tissues. The objective of this study was to identify and characterize potential probiotic bacteria strains from the gastrointestinal tract of free-range locally selected chickens. The bacterial isolates were screened, purified and characterized based on morphological, biochemical and molecular characteristics from 12 well adopted free-range healthy young chickens. Low pH and bile salt tolerance, antagonistic activity, antibiotic activity, hemolysis activity, adhesion to the chicken intestine and carbohydrate fermentation tests was conducted to identify potential probiotic bacteria. Twelve bacterial isolates were screened based on their ability for their tolerance to low pH and bile salt. The isolates were identified by using 16S rRNA gene partial sequencing method. All screened isolates showed great survival percentage at low pH, i.e. (89.2±0.75 to 97.1±0.64) survived at 3hrs and (83.6±0.75 to 95.2±0.63) at 6hrs challenge at pH2. Isolate GCM112 was the least tolerant strain in 6.0% salt concentration at 12 and 24hrs exposure time (82.1±1.28 and 79.9±1.96%) respectively. The result revealed no strain tests in this study exhibited α- and β-hemolytic activity when cultured in sheep blood agar. Most isolated strains showed best growth at 37°C temperature and up to 4% NaCl concentration. Based on the reported result from in vitro data, GCH212 and GCM412 isolates were recognized as best potential probiotic bacteria for chicken against pathogens but further studies are needed on in vivo assessment on the health benefits in the real life situation.

Establishing the relations of characteristic aroma precursors and volatile compounds for authenticating Tibetan pork.

Tibetan pork has been favored for its unique aromas, which originate from chemical reactions between characteristic precursors in cooking. The precursors (e.g., fatty acids, free amino acids, reducing sugars, and thiamine) of Tibetan pork ((semi-) free range) from different regions in China, comprising Tibet, Sichuan, Qinghai, and Yunnan, and commercial pork (indoor reared) were compared in this study. Tibetan pork was characterized by higher ω-3 polyunsaturated fatty acids (i.e., C18:3n3), higher essential (i.e., valine, leucine, and isoleucine), aromatic (i.e., phenylalanine), and sulfur-containing (i.e., methionine and cysteine) free amino acids, higher thiamine, and lower reducing sugars. Boiled Tibetan pork exhibited higher heptanal, 4-heptenal, and 4-pentylbenzaldehyde compared with commercial pork. The results from multivariate statistical analysis revealed that precursors combined with volatiles exhibited discriminating capability for characterizing Tibetan pork. The precursors in Tibetan pork exerted a certain effect on characteristic aroma generation, probably arising from promoting chemical reactions in cooking.

Health status, antioxidants and milk quality of goats reared under stall-fed and free range grazing system

The present study was conducted to determine health status and milk quality of goats reared under stall fed and free range grazing system. The research areas reported higher THI throughout the summer. Respiration rate (RR) and heart rate (HR) was significantly higher during summer in both the groups. Both groups had significantly increased neutrophils and eosinophils during the summer and increased Hb, PCV, and lymphocyte during the winter. Stall fed goats had significantly higher MDA during summer. The free range group had significantly higher levels of SOD and GSH in the summer and winter, respectively.Vitamins C and E levels declined during the summer. In both the groups, summer levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and plasma alkaline phosphatase (ALP) were significantly higher but remained within the reference range. During the summer, both groups of goats showed a significant decrease in plasma glucose, triglycerides, and cholesterol. Total proteins (TP) and Albumin both significantly declined during the winter in both the groups. Total immunoglobulins significantly increased during the winter in the free range group. Throughout the winter and the summer, respectively, the fat percentage increased in the milk of the stall-fed and free-range groups. Moreover, solid not fat (SNF) significantly declined in stall fed group during the winter. Also, there was no discernible difference in the amount of milk proteins between the groups; however, during the summer, it was discovered that stall-fed goats had more of them. The milk lactose significantly increased in both the groups in the time of summer though the increase was remarkable in stall fed goats. The present study indicated that stall fed and free range reared goats in winter and summer exhibit physiological variation in their health state, milk production, and composition.

Occurrence Characteristics of Chang 7 Shale Oil from the Longdong Area in the Ordos Basin: Insights from Petrology and Pore Structure

Organic geochemistry experiments, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), N2 adsorption, CO2 adsorption, and two-dimensional nuclear magnetic resonance (2D NMR) were performed on ten Chang 7 shale samples (Longdong area, Ordos Basin) to elucidate their pore structure and the characteristics of oil occurrence. Moreover, the factors influencing free oil were discussed, and an occurrence model was established. FE-SEM analysis reveals that the pore types include interparticle pores, intraplatelet pores within clay aggregates, rare fracture pores, and organic matter (OM) pores. The pores are predominantly slit-shaped. The development of micropores was mainly contributed to by OM. Quartz and clay minerals influence the development of macropores and mesopores, feldspar mainly controls macropore development, and pyrite most strongly affects micropore development. Micropores and mesopores constitute the main total pore specific surface area, while mesopores and macropores are the main contributors to the total pore volume. Pores > 2 nm are the main storage spaces for shale oil, and free oil mainly occurs in pores > 20 nm. Adsorbed oil and free oil were assessed by NMR T1–T2 mapping. The adsorbed oil signal intensities range from 7.5–23.4 a.u. per g of rock, and the free oil signal intensities range from 4.4–23.2 a.u. per g of rock. The free oil proportions are 15.9–70.6% (average of 44.2%). The free oil proportion is negatively correlated with the clay mineral content and total organic carbon (TOC) content but positively correlated with the saturated hydrocarbon content and volume of pores > 20 nm. The results of this study could help optimize favorable shale oil target areas.

Design and analysis of 4-bit low-power, high-performance current steering DAC implemented using CNT-GDI logic

This work proposes a 4-bit unary weighted current steering digital to analog converter (CS-DAC) with minimal integral nonlinearity (INL) error, less power consumption, and low glitch area. The input to the differential switch is through the thermometer decoder designed using GDI (Gate Input diffusion) logic, which lowers the power consumption and glitch area (also known as glitch energy) to 7.7nW and 0.0038 pVs respectively. Additionally, this unary decoder is used in the digital block of the proposed CS-DAC to lower the overall space and power consumption. The proposed circuitry employs 32 nm technology CNTFET technology. The comparative analysis of the CNTFET based proposed unary decoder design has shown a significant improvement of various performance measuring parameters. The power consumption, latency, and PDP has got substantially reduced by ∼94 %, ∼86 %, and ∼99 % in comparison to the conventional MOSFET based circuitry. In addition, it has been observed that in the proposed CNTFET GDI logic-based CS-DAC the INL, and differential non linearity (DNL) have got decreased by ∼89 % and ∼92 % respectively in comparison to the conventional MOS-based CS-DAC.Further, the dynamic performance measuring parameter like Spurious Free Dynamic Range (SFDR) has got increased by 41.7 % in comparison to the conventional CS-DAC. With a single 0.9 V supply voltage, the proposed DAC dissipates ∼100 % less power as compared to conventional CMOS-based CS-DAC.

Wide Range Si3N4 Optical Sensor Using Cascaded Mach-Zehnder Interferometer Coupled Ring Resonator

Abstract A cascaded MZI-coupled ring resonator (CMCR) optical sensor based on silicon nitride waveguide is proposed. The sensor is composed of a large size microring and a bus waveguide coupled with the microring for four times. The three-segment bus and the microring waveguide between the four coupling regions form three MZI of the same size, forming a three-stage cascaded MZI. The resonant effect of the cascaded MZI transforms the smooth comb spectrum of the microring into a V-shaped comb spectrum, expands the effective free spectral range of the ring resonator, and realizes the cascaded MZI-coupled microring sensor with a large measurement range. For the cascade MZI-coupled microring with a radius of 100μm, the effective free spectrum range is 53.7nm, which is more than 24 times larger than that of the all-pass microring of the same size. The refractive index sensitivity is 339.49 nm RIU/and the detection limit is 2.95 × 10−5 RIU. these excellent properties prove the feasibility of cascaded MZI-coupled microring structure for large measurement range sensing, and are expected to be used in large refractive index range sensing applications in the future.

Gradual loaded exercise of knee extension muscles using an orthosis after wide resection of a femoral sarcoma and quadriceps muscle: a case report

BackgroundDetails of improved gait ability after wide resection of soft tissue sarcomas that necessitate removal of portions of the quadricep muscle have not yet been reported. We describe a patient with improved gait ability following a rehabilitation program after wide resection of a soft tissue sarcoma that included four components of the quadricep muscle.Case presentationAn 85-year-old Japanese man underwent wide resection of an undifferentiated pleomorphic sarcoma that included portions of the quadriceps femoris muscle. The rectus femoris, vastus medialis, sartorius, and vastus intermedius were separated in the maximally bulging region of the tumour. Three weeks postoperatively, gait exercise was initiated using a rigid knee orthosis with a dual-adjustable lock knee. The contraction loading of the knee extension muscle was controlled by adjusting the hinge motion range of the orthosis as follows: fully extended, fixed knee 0°–30°, and free range. Under this regimen, he could walk independently without a rigid orthosis within 5 weeks postoperatively but could not sit on his heels during daily living activities. At six months, there was no clinical evidence of recurrent tumours or complications.ConclusionsPostoperative gait ability might be affected by not only the number of resected muscles but also by the function of the separated muscles and the cross-sectional area of the remaining muscle. Gradually loaded exercise of the knee extension muscles using an orthosis could result in an improved gait motion for patients who undergo wide resection of a sarcoma that includes four components of the quadriceps femoris.

Comparison of Effectiveness of Maitland’s Mobilizations at End-Range Versus Within Pain-Free Joint Range of Movement in Treatment of Patients with Frozen Shoulder RCT

Maitland’s mobilizations are an important treatment option for treating patients with Frozen shoulder or Adhesive capsulitis of shoulder joint. In this study we compared the efficacy of Maitland’s mobilization at end-range of tissue resistance with high intensity glides i.e. High Grade with low intensity glides i.e. Low grade within pain free range of joint movement. The study was conducted at OPD of Department of Physical Therapy, Mayo Hospital, Lahore. In this study total of 57 patients were taken and only 36 patients were selected after assessing on inclusion criteria. Those 36 patients were divided into 3 groups with 12 patients in individual group. With the joint close to its neutral position, translation and distraction techniques were used to begin mobilization techniques in the fundamental starting positions. Numeric Pain Rating Scale (NPRS) and SPADI scales were used for measuring pain and disability index. ROMs were measured before, during and after treatment course i.e. at Day 1, after 2 weeks and after 4 weeks respectively. Statistical analysis was done on SPSS using ANOVA. When the ranges were compared by using ANOVA on SPSS it was evident that Group A patients showed better treatment outcomes as compared to other two groups. P-value (0.000) less than 0.05 is considered significant for group A patient. Mean of demographic data also support the fact that AC is more common in women than man. As a result of this study we can conclude that all treatment options gave good results in enhancing range of motion (ROM) and decreasing pain in shoulder joint but high-grade mobilization techniques applied at end range gave a significant treatment outcome on Ranges when compared. Numeric Pain Rating Scale (NPRS) and SPADI scales

Molecular Docking, MM-GBSA, and Molecular Dynamics Approach: 5-MeO-DMT Analogues as Potential Antidepressants.

Since depression is a common mental illness affecting an estimated 5% of people worldwide, investigators are encouraged to develop effective antidepressants. According to the monoamine-deficiencyhypothesis, the underlying pathophysiology of depression is a deficiency of some neurotransmitters (serotonin, norepinephrine, or dopamine)in the central nervous system. The neurotransmitter serotonin has drawn the most attention concerning depression. As per research, 5-methoxy-N, N-dimethyltryptamine (5-MeO-DMT) elevates inter-synaptic serotonin levels when administered as a single inhalation of vapor from dried toad secretion and leads to higher life satisfaction, convergent thinking, higher ratings of mindfulness, lower ratings of depression, and anxiety. Furthermore, although 5-MeO-DMT lowers stress biomarkers such as cortisol, it is a psychedelic with hallucinogenic effects. In the present study, analogues of 5-MeO-DMT are designed with the hope that they might have better therapeutic activity and lower psychedelic side effects. The current study aimed to look at 5-MeO-DMT analogues as possible antidepressants. We used 70,000 5-MeO-DMT analogues that were sketched using Marvin to conduct a High Throughput Virtual Screening method in hopes of finding potential 5-MeO-DMT analogues against the 5-Hydroxytryptamine 1A receptor (5-HT1AR; 7E2Y.pdb) as an agonist. The prediction of the analogue-protein interaction and the evaluation of the binding affinity is accomplished by employing molecular docking. The Glide XP docking data indicated that a total of 21 compounds had Glide gscores ranging from -11.41 to -6.53 kcal/mol. When compared to the standard 5-MeO-DMT with the binding affinity of -7.75 kcal/mol, 14 compounds showed better binding affinity. Furthermore, Molecular Mechanics -Generalised Born and Surface Area solvation (MM-GBSA) indicated a binding free energy range of -63.55 to -35.37 kcal/mol, and 18 compounds showed better binding free energy than standard 5-MeO-DMT (-41.42 kcal/mol). Through ligand binding interactions with Asp116, Phe361, Phe362, Ser190, Ser199, Val117, Trp358, Ala365, Pro369, Ile189, Tyr195, Ala203, Ile167, Tyr390, Cys120, Trp358, Val364, Ala365, and Leu368, these complexes were stabilized, according to the molecular dynamic simulation of 20453/7E2Y in 100ns.