The main objective of this simulation was to estimate fluid characteristics due to the presence of holes in the perforated helical type static mixer using Computational Fluid Dynamic (CFD) during the process of biodiesel production with static mixing reactor. The conventional helix compared to the perforated helix with three scenarios of holes, namely with one hole each side of the helix as 1st scenario, two holes each side of the helix as 2nd scenario and three holes each side of the helix as 3rd scenario. Simulated CFD of biodiesel production showed the mixing process with the help of perforated helical type static mixers provided a better mixing impact compared to conventional helix. The concentration of fatty acid methyl ester (FAME) using perforated helical type (as much as 86.26%) was higher than conventional helical type (as much as 82.79%). In other word the number of FAME concentration was increased by 4.06% due to the presence of perforated helix. While, the viscosity was decreased by 15.33%.