Different Concentrations Of Extract Research Articles

Aktivitas Antibakteri Ekstrak Sargassum plagyophyllum terhadap Bakteri Listeria monocytogenes dan Pseudomonas aeruginosa

Bakteri Listeria monocytogenes dan Pseudomonas aeruginosa merupakan bakteri patogen yang harus dihambat atau dibunuh pertumbuhannya menggunakan senyawa antibakteri. Sargassum sp. merupakan salah satu jenis rumput laut cokelat yang memiliki komponen senyawa bioaktif yang dapat berperan sebagai antibakteri. Penelitian ini bertujuan untuk memperoleh komponen bioaktif dan aktivitas antibakteri ekstrak Sargassum plagyophyllum. Penelitian ini terdiri dari maserasi rumput laut S. plagyophyllum dan karakterisasi ekstrak yang meliputi screening fitokimia dan analisis antibakteri gram positif dan negatif pada konsentrasi ekstrak yang berbeda yaitu 2, 4, dan 6% w/v. Ekstrak metanol S. plagyophyllum memiliki rendemen 1,32±0,04% dan mengandung senyawa bioaktif alkaloid, steroid, saponin, dan fenolik. Jika dilihat dari aktivitas antibakteri, diperoleh bahwa ekstrak S. plagyophyllum memiliki zona hambat terhadap bakteri gram positif (L. monocytogenes) dan bakteri gram negatif (P. aeruginosa), namun diameter penghambatan pertumbuhan bakteri gram negatif (6,67±0,58 mm) lebih besar dibandingkan dengan bakteri gram positif (4,67±0,58 mm) pada konsentrasi ekstrak tertinggi (6%).

Spectrophotometric Studies of Indolic Compounds from Vinca Minor L.

Abstract The most medically representative plant in the Apocynaceae family is Vinca minor. Vinca minor is a perennial, herbaceous plant, commonly known as Saschiu. In the present research we aimed to isolate the indole compounds from Vinca minor L. and to carry out some physico-chemical studies on these compounds: UV-VIS spectroscopy and the determination of the polyphenols content using Folin-Ciocâlteu method. The spectrophotometric study of the alcoholic plant extracts obtained from the leaf and stem of Vinca minor was performed using the UV-VIS spectrophotometric method and a VWR UV-630PC double beam spectrophotometer. Both samples of plant alcoholic extracts obtained from the leaf and from the stem of Vinca minor L. had the specific absorption maxima detected in the range 225-350 nm and the absorbance maximum in both cases was 3.5 (u.a- absorbency units). The total content of polyphenols was determined by the Folin-Ciocâlteu method from alcoholic extracts of different concentrations: 40%, 70%, 96% (T40, T70, T96, F40, F70, F96) obtained from the leaf and stem of Vinca minor plant using the spectrophotometer model JASCO- 550 UV VIS. Regarding the alcoholic extracts obtained from the leaf of Vinca minor L., the concentrations of polyphenols were between 812.50 mg / 100g pv and 1737.50 mg / 100g pv and and in the case of alcoholic extracts obtained from the strain, the results were between 1525.00-3962.50 mg GAE / 100 g pv, results that were in accordance with the literature.

Effect of Vernonia amygdalina on phospholipase activity from Naja Mossambica (Cobra)

Background: Phospholipases are one of the numerous enzymes found in the Naja mossambica venom. They play a major role in snakebite envenomation, and also responsible for the hydrolysis of a phospholipid, disrupting the membrane integrity. In this study, we evaluated the effect of Vernonia amygdalina on Phospholipase activity from Naja mossambica (Cobra) Results: Partially purified phospholipase had maximal velocity (Vmax) and Michaelis Menten constant (Km) of 7.6 × 10-5 mol/min and 1.7mg/ml, while the crude phospholipase had Vmax and Km of 9.4 × 10-5mol/min and 2.5mg/ml respectively. Inhibition study of aqueous extracts of Vernonia amygdalina leaf shows that the extract is a potent inhibitor of crude phospholipase in a dose-dependent pattern. The different doses of extract 15 %, 10 % and 5% produced percentage inhibition of 74.04 %, 78.6 % and 86.63% respectively. The kinetic binding constant (Ki) values of crude phospholipase for different concentrations of extracts 5%, 10% and 15% were 0.21mg/ml, 0.29mg/ml and 0.39mg/ml, while the partially purified phospholipase for different concentrations of extracts 5%, 10% and 15% were were 0.48mg/ml, 0.42mg/ml and 0.41mg/ml respectively. It can be deduced from the results that the extract inhibits the phospholipase activity in an uncompetitive manner. Conclusion: Aqueous extract of Vernonia amygdalina leaves may contain some bioactive agents that could serve as potent inhibitors of phospholipase from Naja mossambica venom.

The Effectiveness of Ethyl Acetate Extract From Breadfrui (Artocarpus Altilis) Leaves to Inhibit Diarrhea-Causing Bacteria

Diarrhea is a health problem that commonly occurs in developing countries. Bacteria that cause diarrhea are among others Escherichia coli, Salmonella typhosa and Staphylococcus aureus. This research aimed to investigate the diameters of inhibition zones of breadfruit leaf (A. altilis) ethyl acetate extract in different concentrations against the growth of Escherichia coli, Salmonella typhosa and Staphylococcus aureus. The research applied an experimental laboratory by using a post-test control group design. This research was performed at the Bacteriology Laboratory of STIKES Nasional by using the diffusion disk method. The research showed the radical zone diameters against Escherichia coli with the concentrations of 20%, 40%, 60%, 80%, and 100%, were 6.16 mm, 6.41 mm, 6.74 mm, 7.49 mm, and 7.79 mm, respectively. The inhibition zones against Staphylococcus aureus were 8.15, mm 9.43 mm, 10.29, 10.38 mm and 11.42 mm, while against Salmonella typhosa were 7.94 mm, 8.87 mm, 10.15 mm, 10.26 mm, and 11.23 mm, respectively. The results of the ANOVA test showed the p-value=0.00 and the results of the LSD test revealed the differences in the inhibition effects of A. altilis leaf extract against the growth of E. coli, S. typhosa and S. aureus. This study concludes that concentration variations of Artocarpus altilis leaf ethyl acetate extract can inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhosa.

Botanicals as Selective Pesticides for the Integrated Pest Management in Vegetables: A Review

The over-dependence on chemical pesticides for the management of insect pest have an adverse effect on the environment and have been a matter of concern, so plant extracts which are also known as green pesticides can be an alternative source of chemical pesticides due to their safety, eco-friendly and many other properties. The current study aimed to study the different botanicals which are effective against some pest of important vegetables. The different types of plant extract used as biocides such as neem, garlic, tobacco, lantana, pongamia, khuksha, ginger and many others are being utilized to control and manage the pest or disease of different plants. The neem extract of different concentrations was found effective in controlling pest of vegetables like brinjal, okra, chilli and cabbage. Botanical like Eupatorium, the mixture of kerosene emulsion and pyrethrum dust, Pongamia extract, Lantana leaf extract, Tobacco extract and Garlic extract, seed extract of Annona squamosa act as a potential insecticide to vegetable pests and play an important role in the Integrated Pest Management for sustainable agriculture.

Cytotoxic effect of define concentration of Yarrow (Achillea millefolium) extract used in Iranian traditional medicine on AGS human gastric cancer cell-line

Gastric cancer is one of the main cancer-related death causes in the world threatening almost 12 million human lives by 2020. Achillea millefolium L. (yarrow) with domestic Iranian name of Bumadaran, has been as a curative plant for several medical conditions for a long time. Its antimicrobial and wound healing effects have been reported and in this study we aimed to survey the in-vitro cytotoxic and anti-cancer effects of this plant. After taxonomically identification of Achillea millefolium L., its hydroalcoholic extract was extracted and the AGS gastric cancer and L-929 normal fibroblastic cell-lines with treated by the different concentrations of extract in 3 time periods (24, 48, and 72 h). MTT assay was performed for the evaluation of cytotoxic effects. The 24 h treatment did not affect cell survival notably, while the concentrations of 64 and 16 µg/ml were determined as IC50 concentrations at 48 and 72 h incubation times respectively. The 72 h incubation time with 16 µg/ml showed the best effectiveness on cancerous cell-line while being safe for normal cell-line. The long-term treatment of AGS cancer cell-line by low concentrations of yarrow extract could be useful for the cytotoxicity upon this type of cancerous cells.

Assessment of Antimicrobial Activity of Pomegranate, Cranberry, and Black Chokeberry Extracts against Foodborne Pathogens.

Herbal and plant extracts are being applied to a wide range of foods against different types of foodborne pathogens. In the present study, ethanolic and aqueous extracts of different concentrations (5% v/v, 10% v/v, and 20% v/v) from cranberry (Vaccinium macrocarpon), black chokeberry (Aronia melanocarpa), and pomegranate (Punica granatum L.) plants were applied in five concentrations (62.5 to 1000 mg/mL) against foodborne strains of Staphylococcus aureus, Escherichia coli, and Streptococcus pyogenes. The results revealed that a low concentration of solvents (5% v/v) did not exhibit decreased antimicrobial activity in comparison with higher solvent concentrations (10% and 20% v/v). Additionally, both aqueous and ethanolic extracts were highly effective against pathogens even in their low concentrations (62.5 mg/mL and 125 mg/mL). Likewise, the extracts exhibited promising results (aqueous extracts of pomegranate, cranberry, and black chokeberry in a food-compatible concentration of 2% w/v) were applied to raw pork meatball production, and their antimicrobial activity was recorded versus Enterobacteriaceae, total mesophilic bacteria (TMB), lactic acid bacteria (LAB), Staphylococcus spp., Pseudomonas spp., and yeasts/molds. The outcome demonstrated that meatballs that contained aqueous extracts of pomegranate were more resistant to spoilage compared to all of the other samples, as it was preserved for more days. Likewise, these extracts of a plant origin could be used as natural preservatives in meat products, even in their low concentrations.

Pengaruh Perbedaan Konsentrasi Ekstrak Karapas Udang Terhadap Sifat Fisikokimia Kaldu Bubuk yang Dihasilkan

<em>Shrimp carapace is one of the fisheries waste products that can be a potential alternative for natural flavoring because it contains a lot of glutamic acid that create umami taste in dishes by turning it into a powdered broth. Powdered broth is a product that has been known as a food additive that is obtained from boiling meat or more commonly known as a seasonings. The purpose of this study is to know how the effect and best treatment of different concentrations of extract from shrimp carapace on the quality of the resulting powder broth. This research was conducted at the Food Engineering Laboratory, Chemistry Laboratory Semarang university and the Chemix Pratama Laboratory Yogyakarta. The design of the experiment used was a completely randomized design with 5 treatments and 4 replications, which gives the concentration of shrimp carapace, which is 3%; 3.5%; 4%; 4.5%; And 5%. The test parameters were water levels, fat levels, protein levels, glutamate levels, and hedonic tests include taste and aroma attributes. To find out the difference between treatment, data was tested with Duncan multiple range test (DMRT) at 5% level. The results have shown that differences in shrimp carapace extract have a significant effect (p <0.05) on water levels, fat levels, protein levels, glutamate levels, and hedonic scores. This study recommends P3 treatment as the best alternative treatment considering water level (4,17%), fat content (1,29%), protein (15,05%), glutamate (10,76%), and being the most preferred by panelists. with the taste attribute score (5.50), and aroma (4.77).</em>

Effectiveness of Anonidium mannii seed extracts against Colletotrichum lindemuthianum and Fusarium solani, agents responsible for anthracnose and fusariose of common beans (Phaseolus vulgaris L.) in the Centre region-Cameroon

Anthracnose and Fusariose of beans caused by Colletotrichum lindemuthianum and Fusarium solani respectively are two dreaded diseases that can lead to yield losses of 30 to 100% in the field. The present study was carried out to evaluate the antifungal activities of seed extracts of Anonidium mannii (Annonaceae) on the in vitro growth of C. lindemuthianum and F. solani. Extracts of A. mannii seeds obtained from acetone, water and methanol solvents, were used for phytochemical screening and for the preparation of solutions with concentrations of 12.5, 25 and 50 µl/ml. To evaluate radial growth, explants of 0.7 cm diameter each were obtained from two strains of C. lindemuthianum and two strains of F. solani. They were placed each at the centre of sterile Petri dishes containing the PDA medium supplemented with the different concentrations of extracts and incubated at 23°C for 6 days. The results of the phytochemical screening revealed the presence of alkaloids, phenols, saponins in the A. mannii seed extracts and this depended on the solvent used. The minimum inhibitory concentrations (MIC50 and MIC90) were also calculated. At a concentration of 50µl/ml each, acetone extract inhibited the growth of strain 2 of C. lindemuthianum (2.33 cm) more than the methanol extract (3.26 cm) in six days of incubation. Similarly, the growth of strain 2 of F. solani was strongly inhibited by methanol extract (1.98 cm). The MIC50 of the aqueous extract gave 27.38 µl/ml with F. solani and 32.24 µl/ml for the acetone extract with C. lindemuthianum. Due to their antifungal power, after purification with bioactive compounds, the aqueous, acetone and methanol extracts revealed by the phytochemical screening could be used as synthetic fungicides for the control of anthracnose and fusariose of common bean.

Antimicrobial activity of Spondias mombin L. aqueous and hydroethanolic extracts on Enterococcus faecalis and Pseudomonas aeruginosa - an in vitro study

Objective: To chemically characterize Spondias mombin L. extracts and to evaluate the in vitro antimicrobial activity of these extracts on Enterococcus faecalis and Pseudomonas aeruginosa. Material and methods: Both extracts were submitted to phytochemical prospecting and thin layer chromatography (TLC) analysis prior to determination of Minimum Inhibitory Concentration (MIC), Minimum Inhibitory Adhesion Concentration (MIAC) and Bactericidal Kinetics (BK). The Student’s t-test was used to compare MIC results at the 5% significance level. The chemical characterization, MIAC and BK data were analyzed descriptively. Results: Phenolic compounds, flavonoids, tannins and saponins were detected in the extracts. The hydroethanolic extracts in different concentrations showed statistically superior inhibitory bacterial growth activity (p <0.05) compared to the control against the two investigated microorganisms, whereas the aqueous extracts (also in different concentrations) was statistically superior (p <0.05) to the control only against Pseudomonas aeruginosa. The anti-adherent effect of both extracts was superior to that of the control for P. aeruginosa, and similar to that for E. faecalis. In their crude and diluted concentrations, aqueous and hydroethanolic extracts showed bactericidal action within the first two to four hours of contact with the bacteria. Conclusion: Spondias mombin L. extracts in different concentrations showed superior antimicrobial activity than 0.12% chlorhexidine digluconate against the microorganisms with this activity associated to the chemical characterization of these extracts.

Potencial alelopático do extrato aquoso de Prosopis juliflora (Sw.) DC sobre a germinação de Bidens pilosa L.

RESUMO: A algaroba (Prosopis juliflora) é uma espécie que possui alto valor econômico na alimentação animal, destacando-se principalmente o consumo de suas vagens. Além disso, tem se mostrado como alternativa para o controle de plantas invasoras, favorecendo a redução tanto no uso de defensivos químicos com no risco de contaminação. O objetivo da pesquisa foi investigar o potencial alelopático de diferentes concentrações do extrato proveniente de folíolos de P. juliflora sobre a germinação da espécie invasora B. pilosa. Foram utilizadas cinco concentrações, 0 (controle); 25; 50; 75 e 100%, do extrato de P. juliflora, com quatro repetições de 25 sementes cada. Avaliou-se a porcentagem; a velocidade; o índice de velocidade e o tempo médio de germinação. O extrato de algaroba não afeta negativamente o processo germinativo de sementes de picão preto, não sendo indicado, portanto, para o controle desta planta invasora.
 PALAVRAS-CHAVE: Alelopatia, germinabilidade, picão-preto.

The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa

Abstract. Nugroho LH, Dewatisari WF, Retnaningrum E, Purwestri YA. 2021. The potency of Sansevieria trifasciata and S. cylindrica leaves extracts as an antibacterial against Pseudomonas aeruginosa. Biodiversitas 22: 408-415. Sansevieria trifasciata and Sansevieria cylindrica are the major herbs in Indonesia, which contain several bioactive compounds as potential sources of antibacterial agents. This study aims to evaluate the antibacterial activity of S. trifasciata and S. cylindrica leaves extract and fraction against Pseudomonas aeruginosa and to identify its bioactive compounds. Crude ethanolic extract of S. trifasciata and S. cylindrica leaves were tested for their antimicrobial activity by disk diffusion method against P. aeruginosa. S. trifasciata showed strong antibacterial activity with an inhibition zone of 18.3 mm compared to S. cylindrica. Different concentrations of extract i.e. 4 mg/mL, 8 mg/mL, 16 mg/mL, 32 mg/mL, 64 mg/mL, 128 mg/mL and 256 mg/mL were tested for their minimum inhibitory concentrations (MIC). The MIC results showed that the S. trifasciata extract was able to inhibit bacterial growth at a concentration of 32 mg/mL. Results of vacuum liquid chromatography (VLC) and thin-layer chromatography (TLC) revealed that only fraction 3 showed the highest antibacterial activity at 16 mg/mL. In TLC bioautography analysis, fraction 3 showed a clear zone at Rf 0.93. The phytochemical analysis showed that terpenoid, phenolic, triterpenoid, and flavonoid compounds were found in S. trifasciata extract that were associated with antibacterial activity.

Uticaj ekstrakta Ambrosia artemisiifolia na klijanje i rast klijanaca Pisum sativum L. i Phaseolus vulgaris L.

Previous research has shown that there are allelopathic interactions between plants, releasing allelochemicals for which it is still unknown whether they are randomly generated or transmitted from generation to generation. In this paper, the influence dried ragweed leaves (Ambrosia artemisiifolia L.) aqueous extract of different concentrations: concentrated extract (10%), extract diluted to 1/2 (5%); 1/4 (2.5%) and 1/8 (1.25%) on germination and growth of peas and beans was monitored. The results showed that the increased concentration of ragweed extract inhibited germination, prolonged germination time and decreased the germination index of both test plant species compared to the control. The ragweed extract (concentrated and 1/2) reduced the growth of shoots and roots compared to the control (by more than 50%), with the inhibitory effect being more visible on young pea plants compared to beans. The results of the research showed that the presence of ragweed as a weed in the field significantly reduces the germination and growth of cultivated plants, which is why care should be taken to suppress and remove it in a timely manner.

In Vitro and In Vivo Evaluation of Nanostructured Biphasic Calcium Phosphate in Granules and Putty Configurations.

Synthetic biphasic calcium phosphate (BCP) granules and powder are biocompatible biomaterials with a well-known capacity for osteoconduction, presenting very satisfactory clinical and histological results. It remains unanswered if the putty configuration impacts the biological response to the material. In this study, we aimed to compare the cytocompatibility and biocompatibility of nanostructured BCP in the putty configuration (moldable nanostructured calcium phosphate, MnCaP) on the healing of critical-sized bone defects (8 mm) in rat calvaria. Cytocompatibility was determined through the viability of fibroblast cells (V-79) to the extracts of different concentrations of MnCaP. Forty-five Wistar rats were randomly divided into three groups (n = 15)—clot, MnCaP, and commercial biphasic calcium phosphate in granules configurations (Nanosynt®)—and subdivided into three experimental periods (1, 3, and 6 months). Histological, histomorphometric, and microtomographic analyses allowed the evaluation of newly formed bone, residual biomaterial, and connective tissue. The in vitro evaluation showed that MnCaP was cytocompatible. The histomorphometric results showed that the Nanosynt® group granted the highest new-formed bone values at six months (p < 0.05), although the biomaterial volume did not differ between groups. The putty configuration was easier to handle, and both configurations were biocompatible and osteoconductive, presented similar biosorption rates, and preserved the calvaria architecture.

Evaluation of antioxidant, cytotoxic, antibacterial effects and mineral levels of Verbascum lasianthum Boiss. ex Bentham.

The present study aimed to determine the antibacterial, antioxidant, cytotoxic activities and element levels of Verbascum lasianthum Boiss. ex Bentham. The free radical scavenging activity, total phenolic content, total antioxidant capacity, total oxidant capacity levels were analyzed as the antioxidant parameters. Seven bacteria and one yeast strains were used to determine the antimicrobial activity. The cytotoxic effects of plant extracts were determined using A549, MCF-7, HepG2 and SH-SY5Y cell lines. The findings demonstrated that the antioxidant activity increased with an increase in the phenolic content of extracts. This species is rich in bio-elements such as Fe, Cu, Mn, Zn, and Mg. Different concentrations of extracts could have antibacterial activity. This plant had an apparent cytotoxic effect only in the A549 cell line and increased the proliferation in other cell lines. The findings demonstrated that plant could be used alone or as a supplement to the current treatment protocols in diseases due to their antioxidant, antibacterial and cytotoxic effects. However, it is recommended that Verbascum L. species intended for use in therapy should be procured from areas where there is no soil pollution or organic farming is preferred.

An Overview of Phytochemical and Biological Activities: Ficus deltoidea Jack and Other Ficus spp.

ABSTRACTFicus deltoidea Jack (Moraceae) is a well-known medicinal plant used in customary medication among the Malay people to reduce and mend sicknesses such as ulcers, psoriasis, cytotoxicity, cardioprotective, inflammation, jaundice, vitiligo, hemorrhage, diabetes, convulsion, hepatitis, dysentery injuries, wounds, and stiffness. Ficus deltoidea contains a wide variety of bioactive compounds from different phytochemical groups such as alkaloids, phenols, flavonoids, saponins, sterols, terpenes, carbohydrates, and proteins. The genus Ficus has several hundreds of species, which shows excellent therapeutic effects and a wide variety of helpful properties for human welfare. Searching information was collected by using electronic databases including Web of Science, Science Direct, Springer, SciFinder, PubMed, Scopus, Medline, Embase, and Google Scholar. This review is, therefore, an effort to give a detailed survey of the literature on its pharmacognosy, phytochemistry, phytochemical, and pharmacological properties of Ficus and its important species. This summary could be beneficial for future research aiming to exploit the therapeutic potential of Ficus and its useful medicinal species.

Biological activity of geopropolis produced by Partamona cupira (Meliponinae, Apidae) in the semiarid of the Brazilian northeast

Research on the chemical composition and pharmacological activities of geopropolis produced by stingless bees (Hymenoptera, Apidae, Meliponini) may contribute to expand its use of propolis-based formulations in the clinical context. Thus he study aimed to evaluate the chemical composition and biological activity of the hydroethanolic extract (HEG) of the geopropolis of Partamona cupira, obtained in the semiarid region of northeast Brazil. Chemical analyses of HEG were carried out using HPLC-DADESI-MS/MS. The antioxidant activity of extracts was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay method and cytotoxic activity by the in vitro MTT method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The antibacterial activity of the HEG was evaluated through the disc-diffusion test on agar and measurement of the promoted by the extract in different concentrations. The genoprotective potential of the HEG was evaluated through the comet assay on fibroblasts of L929, co-treated with the extract and submitted to genotoxicity induction with H2O2. We also investigated the healing effect of the cream containing geopropolis (10%) on experimental skin wounds in Wistar rats. The HEG presented in its composition phenolic compounds of high biological activity, as well as revealed high antioxidant activity and promoted genoprotective effect by reducing DNA damage from L929 fibroblasts. The HEG presented antimicrobial activity promoting inhibition of S. aureus, S. pyogenes, E. coli and E. aerogenes. The topical use of the cream containing geopropolis promoted wound closure and faster reepithelialization in relation to the control group, in addition to a less intense inflammatory reaction, increased fibroblastic proliferation and collagen deposition.

Antimicrobial Effect of Arrabidaea chica Polyphenolic Extract Used as Dentin Pre-treatment against Cariogenic Microbiota

Aims: This study tested the antimicrobial effect of Arrabidaea chica (Ac) polyphenolic extract in different concentrations incorporated into an aqueous solution or into a primer of an adhesive system used for cavities pre-treatment against Streptococcus mutans (Sm) and Lactobacillus casei (Lc).&#x0D; Study Design: In vitro experimental study design to evaluate the minimum inhibitory concentration (MIC) of different solutions containing Ac against Sm and Lc.&#x0D; Methodology: Aqueous solution and primer (from a self-etching adhesive system - Clearfil SE Bond/Kuraray) were prepared containing Ac extract in six different concentrations: 0.0% (null); 0.25%; 0.5%; 1.0%; 1.5%; 2.0%; 2.5%. To determine the MIC, tubes with the inoculates of Sm and Lc were added with the solutions described above. Serial dilutions were inoculated in plates with culture medium (n=3). The colony forming unit count (CFU/mL) was carried out after 48 hours. Negative controls (aqueous or primer solutions without Ac incorporation) were used in both tests.&#x0D; Results: Regression model showed that all Ac concentrations inhibited Sm growth (p&gt;0.05) in aqueous solution. Negligible or no Sm growth was observed in the presence of primer in Ac concentrations above 0.25% (p&lt;0.05). Negligible or no Lc growth was found in aqueous solution for all Ac concentrations, and in the presence of primer in Ac concentrations above 1% (p&gt;0.05). All concentrations of Ac tested here maintained the acidic characteristics of the solutions.&#x0D; Conclusion: Arrabidaea chica extract into an aqueous solution shows antimicrobial effect against Sm and Lc in all concentrations tested. When incorporated into the primer, the antimicrobial activity is observed in concentrations above 1%.

Application of Plant Extracts to Control Postharvest Gray Mold and Susceptibility of Apple Fruits to B. cinerea from Different Plant Hosts

Sustainable plant protection can be applied on apples against fungal pathogens such as Botrytis cinerea (which is responsible for gray mold)—a significant global postharvest disease. This pathogen can affect a wide range of hosts; and fruits may have variable susceptibilities to B. cinerea from different plant hosts. New possibilities to control gray mold in food production are under demand due to the emergence of resistance against antifungal agents in fungal pathogens. Cinnamon, pimento, and laurel extracts were previously assessed for antifungal activities under in vitro conditions and were found to have the potential to be effective against postharvest gray mold. Therefore, this study aimed to investigate the antifungal activity of cinnamon, pimento, and laurel extracts in vitro and against postharvest gray mold on apples to determine the susceptibility of apple fruits to B. cinerea from different plant hosts, and to analyze the chemical composition of the extracts. Apples (cv. “Connell Red”) were treated with different concentrations of extracts and inoculated with B. cinerea isolates from apple and strawberry followed by evaluation of in vitro antifungal activity. The results reveal that most of the concentrations of the extracts that were investigated were not efficient enough when assessed in the postharvest assay, despite having demonstrated a high in vitro antifungal effect. Apples were less susceptible to B. cinerea isolated from strawberry. To conclude, cinnamon extract was found to be the most effective against apple gray mold; however, higher concentrations of the extracts are required for the efficient inhibition of B. cinerea in fruits during storage.

Antigenotoxic and Antimutagenic Effects of Andrographis paniculata , a Traditional Medicinal Herb against Genotoxicity of Cyclophosphamide: An In Vitro Study on Human Peripheral Lymphocytes

Andrographis paniculata (family: Acanthaceae) is a medicinal herb—used in Indian system of medicine (Ayurveda, Siddha, and Unani), traditional and folk systems to treat various illnesses. This study examined the phytochemical constituents of ethanol extract from A. paniculata and its protective effect against genotoxicity caused by cyclophosphamide (CPA). Phytochemical screening and estimation of total phenolic content were analyzed using standard methods. The bioactive components from the ethanol extract of A. paniculata (EAP) were analyzed using gas chromatography-mass spectrometry. To investigate the protective effect of EAP against CPA-induced genotoxicity, human peripheral lymphocyte cultures were used. To test the antigenotoxic and antimutagenic effects of EAP, lymphocytes were treated with different concentrations of extract (50∼250 mg/mL) alone and co-treated along with CPA+EAP for 48 h. The cells were analyzed for structural chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) in control, CPA treated, and CPA+ EAP co-treated lymphocytes. Results of the study revealed that the lymphocyte cultures which had 48 h continuous exposure to EAP (50∼250 mg/mL) did not show any significant changes in CAs and SCE frequencies. These results substanti-ate the antimutagenic nature of the extract. Furthermore, the lymphocytes co-treated with CPA along with extract showed a significant reduction in CAs (reduced from 26.50±2.50% to 11.00±1.00%) and SCEs (reduced from 9.92±0.63 per cell to 4.56±0.18 per cell). These results suggest that A. paniculata is protective against CPA induced genotoxicity and put forward its possible use as a supplement with chemotherapeutic drugs.